Larry L Barton

Larry L Barton
University of New Mexico | UNM · Department of Biology

Ph D

About

122
Publications
33,680
Reads
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3,453
Citations
Citations since 2017
13 Research Items
1168 Citations
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
Additional affiliations
May 2008 - August 2016
University of New Mexico
Position
  • Professor Emeritus
August 1972 - May 2008
University of New Mexico
Position
  • Professor (Full)
August 1972 - present
University of New Mexico
Position
  • Professor (Asst. Assoc, Full, Emeritus)
Description
  • Directed Microbiol. Teaching Labs ; was one of the teachers for Gen. Microbiol. Additional course offed included Microbial Physiol. and seminars: Agents of Bioterrorism, Microbial Biotechnol. , Environ. Microbiol.

Publications

Publications (122)
Article
Full-text available
Historically, sulfate-reducing bacteria (SRB) have been considered to be strict anaerobes, but reports in the past couple of decades indicate that SRB tolerate exposure to O2 and can even grow in aerophilic environments. With the transition from anaerobic to microaerophilic conditions, the uptake of Fe(III) from the environment by SRB would become...
Article
Selenium (Se) respiration in bacteria was revealed for the first time at the end of 1980s. Although thermodynamically-favorable, energy-dense and documented in phylogenetically-diverse bacteria, this metabolic process appears to be accompanied by a number of challenges and numerous unanswered questions. Selenium oxyanions, SeO4²⁻ and SeO3²⁻, are re...
Article
Full-text available
Bacillus sp. Abq, belonging to Bacillus cereus sensu lato, was isolated from an aquifer in New Mexico, USA and phylogenetically classified. The isolate possesses the unusual property of precipitating Pb(II) by using cysteine, which is degraded intracellularly to hydrogen sulfide (H2S). H2S is then exported to the extracellular environment to react...
Article
Full-text available
Sulfate-reducing bacteria have been suggested to have an etiological role in the development of inflammatory bowel diseases and ulcerative colitis in humans. Traditionally. bismuth compounds have been administered to alleviate gastrointestinal discomfort and disease symptoms. One mechanism by which this treatment occurs is through binding bacterial...
Conference Paper
Lead (Pb) ranks as a major anthropogenic pollutant because it is used extensively by industry and it has no known biological function. The toxic effects of Pb(II) include inhibition of protein synthesis, alteration of the osmotic balance, enzyme inhibition, nucleic acid damage, disruption of membrane functions and oxidative phosphorylation, and is...
Book
Diversity of sulfate-reducing bacteria, their substrates in the intestine, metabolism of molecular hydrogen, and their trophic relationship with other microorganisms were characterized. Mucosal bacteria of the colon and the role of intestinal microbiota in disease development were described. Microbial communities and hydrogen sulfide concentration...
Article
Full-text available
Agriculture, food industry, and manufacturing are just some of the areas where anaerobic technology can be used. Currently, anaerobic technologies are mainly used for wastewater treatment, solid waste treatment, or for the production of electrical and thermal energy from energy crops processing. However, a clear trend is towards more intensive use...
Chapter
Selenium (Se) is transformed by phylogenetically diverse bacteria following several basic strategies which include: (1) satisfying a trace element requirement for bacterial synthetic machinery (assimilatory metabolism), (2) cellular energy production coupled to oxidation/reduction reactions (dissimilatory metabolism), and (3) detoxification process...
Article
Full-text available
In this review, we focus on the activities transpiring in the anaerobic segment of the sulfur cycle occurring in the gut environment where hydrogen sulfide is produced. While sulfate-reducing bacteria are considered as the principal agents for hydrogen sulfide production, the enzymatic desulfhydration of cysteine by heterotrophic bacteria also cont...
Article
Background: Hydrogen sulfide (H2 S) serves as a mammalian cell-derived gaseous neurotransmitter. The intestines are exposed to a second source of this gas by sulfate-reducing bacteria (SRB). Bismuth subsalicylate binds H2 S rendering it insoluble. The aim of this study was to test the hypothesis that SRB may slow intestinal transit in a bismuth-re...
Conference Paper
Full-text available
Sulfate-reducing bacteria (SRB) have been suggested to have an etiological role in the development of inflam-matory bowel diseases (IBD) and ulcerative colitis in humans and rodents [1]. Studies with mice have also revealed that learning and memory are also impaired by hydrogen sulfide produced by SRB in the gut [2]. Triple therapy with bismuth sub...
Article
Full-text available
Sulfate-reducing bacteria have been implicated in inflammatory bowel diseases and ulcerative colitis in humans and there is an interest in inhibiting the growth of these sulfide-producing bacteria. This research explores the use of several chelators of bismuth to determine the most effective chelator to inhibit the growth of sulfate-reducing bacter...
Chapter
Full-text available
The bacteria and archaea that reduce sulfate to sulfide can transform a variety of metal(loids). The latter include metalloids (As, Se and Te), transition metals (Au, Co, Cr, Fe, Hg, Mo, Mn, Ni, Pb, Pd, Pt, Re, Rh, Tc, V, and Zn), and actinides (Pu and U). The conversions are achieved via (1) use of metal-specific enzymes, (2) cometabolism, i.e., u...
Chapter
Full-text available
Microorganisms produce a wide variety of metallo-nanoparticles. These are deposited in the cytoplasm, periplasm, extracellular area, or on the surface of the cell. These nanoparticles may be the result of energy-conserving dissimilatory metal reduction processes, cell-building assimilatory activities, or co-metabolism. This formation of metal or me...
Article
Full-text available
Sulfur is an essential element for the synthesis of cysteine, methionine, and other organo-sulfur compounds needed by living organisms. Additionally, some prokaryotes are capable of exploiting oxidation or reduction of inorganic sulfur compounds to energize cellular growth. Several anaerobic genera of Bacteria and Archaea produce hydrogen sulfide (...
Article
Bacterial cells do not have many cytoplasmic structures but they do have several nanostructures that have considerable molecular organization. Associated with the plasma membrane of bacteria are the structures that drive the rotation of bacterial flagella and the synthesis of adenosine triphosphate (ATP). These nanostructures perform work for the c...
Book
This book is devoted to nanomicrobiology and the nanosystems of bacteria. The initial chapter discusses some of the controversies in the geochemical and biomedical fields associated with the reports of nanobacteria in the environment. Current knowledge of several internal and surface structures of bacteria is addressed in this book. Included are ch...
Article
Fluorescence activity has been used to identify Desulfovibrio and has been termed the 'desulfoviridin test'. This fluorescence is attributed to the prosthetic group of bisulfite reductase, a key enzyme in dissimilatory sulfate reduction. We have pursued the use of fluorescence measurements to quantify sulfate-reducing bacteria. Cells of D. desulfur...
Article
Full-text available
As determined by transmission electron microscopy (TEM), the reduction of selenate and selenite by Desulfovibrio desulfuricans, a sulfate-reducing bacterium, produces spherical (Se, S) sub-micro particles outside the cell. The particles are crystalline or amorphous, depending on medium composition. Amorphous-like Se-rich spherical particles may als...
Article
Dissimilatory sulfate and sulfur reduction evolved billions of years ago and while the bacteria and archaea that use this unique metabolism employ a variety of electron donors, H(2) is most commonly used as the energy source. These prokaryotes use multiheme c-type proteins to shuttle electrons from electron donors, and electron transport complexes...
Article
Central ThemesIntroductionSampling and Sample StorageMicroscopyCultivation of MicroorganismsMolecular PhylogeneticsCulturing Versus Molecular Techniques: Comparisons from Soil StudiesCommunity Fingerprinting Methods Metagenomics: A New Tool for Answering Community Ecology QuestionsEnvironmental ProteomicsStable-Isotope StudiesSummaryDelving Deeper:...
Chapter
Half TitleTitleCopyrightDedicationContentsPrefaceGlossary
Article
Full-text available
Iron metabolism in plants is essential to maintain optimal growth and iron nutrition is dependent on uptake of iron from the environment and movement of iron in the plant tissues. We have examined the translocation of iron in plant leaves following foliar application of FeEDTA to Vicia faba and Zea mays. Using radiolabeled iron, we observed that ir...
Article
Full-text available
Cultures of Wolinella succinogenes were adapted to grow in the presence of 1 mM or 10 mM . Both selenium salts were reduced to red, amorphous, elemental selenium but only after the culture reached the stationary growth phase. Bacterial cells taken from a culture actively reducing selenium were examined by transmission electron microscopy and were f...
Article
Furniture contaminated with uranium will be disposed of, together with U-bearing waste, in a geological repository such as the WIPP site in New Mexico. It is important to understand the effect of the wooden furniture on the migration of uranium in order to predict long-term behavior of uranium in a geological repository environment. In this paper,...
Book
This book is an interdisciplinary review of recent developments in topics including origin of life, microbial-mineral interactions, and microbial processes functioning in marine and terrestrial environments. A major component of this book addresses molecular techniques to evaluate microbial evolution and assess relationships of microbes in complex,...
Article
Full-text available
Molybdate is an essential trace element required by biological systems including the anaerobic sulfate-reducing bacteria (SRB); however, detrimental consequences may occur if molybdate is present in high concentrations in the environment. While molybdate is a structural analog of sulfate and inhibits sulfate respiration of SRB, little information i...
Article
Full-text available
Chemolithotrophic bacteria that use sulfate as terminal electron acceptor (sulfate-reducing bacteria) constitute a unique physiological group of microorganisms that couple anaerobic electron transport to ATP synthesis. These bacteria (220 species of 60 genera) can use a large variety of compounds as electron donors and to mediate electron flow they...
Chapter
We are at the discovery stage for determining the ability of various bacteria to reduce metals and nonessential compounds. Mechanisms for these reductions generally have not yet been established, and it is apparent that much is unknown. A number of questions pertaining to reduction are raised: Which elements and compounds are reduced at the cell su...
Article
In bacteria, the intracellular metal content or metallome reflects the metabolic requirements of the cell. When comparing the composition of metals in phytoplankton and bacteria that make up the macronutrients and the trace elements, we have determined that the content of trace elements in both of these microorganisms is markedly similar. The trace...
Article
Full-text available
We investigated the influence of the trivalent scandium (Sc), chromium (Cr), gallium (Ga), yttrium (Y) and lanthanum (La) on both the function and activity of ferric chelate reductase (FCR) in cucumber (Cucumis sativus L.) roots. Cucumber seedlings were grown for 1week in a nutrient solution without Fe or in some experiments with 10microM FeEDTA. I...
Chapter
BN bonds;BH Bonds;sodium tetrahydridoborate;sodium hydride;ethylene glycol
Article
INTRODUCTION Toxic heavy metals and metalloids constitute an international pollution problem that not only impacts public health but also is of environmental and economic importance. Prokaryotes with the physiological activity of sulphate reduction are found in a number of environmental sites containing toxic metals and these microorganisms have de...
Book
The sulphate-reducing bacteria (SRB) are a large group of anaerobic organisms that play an important role in many biogeochemical processes. Not only are they of early origins in the development of the biosphere, but their mechanisms of energy metabolism shed light on the limits of life processes in the absence of oxygen. They are widely distributed...
Chapter
Nitrogen-fixing bacteria require iron for rhizospheric growth as well as for plant-Associated symbiotic activities. One of the iron uptake systems involves siderophores and bacteria produce a great diversity of ferric ion chelators in iron-limited environments. This review examines some of the free-living nitrogen-fixing bacteria, including Azotoba...
Book
This book uses an interdisciplinary approach to provide a comprehensive review on the status of iron nutrition in plants. International scientists discuss research on acquisition of iron by strategy I and strategy II plants. These reviews summarize a variety of plant species and include both laboratory and field observations. Topics covered in this...
Article
Full-text available
An experimental study on reduction of U (VI) by anaerobic bacteria, Shewanella putrefaciens, is first reported here in China. The experimental conditions were: 35°C and pH= 7.0-7.4, corresponding to a physicochemical environments in which the sandstone-hosted interlayer oxidation-zone type uranium deposit formed in Northwest China’s Xinjiang. Bacte...
Article
Abstract A simulated experimental reduction of UVI and the synthesis of uraninite by a sulfate-reducing bacteria, Desulfovibrio desulfuricans DSM 642, are first reported. The simulated physicochemical experimental conditions were: 35°C, pH=7.0–7.4, corresponding to the environments of formation of the sandstone-hosted interlayer oxidation-zone type...
Article
This new textbook on bacterial physiology is aimed at senior level students pursuing a one-semester course in the biology or microbiology curriculum. The text takes a balanced view of prokaryotic physiology, discussing both bioenergetics and bacterial metabolism in a way that establishes general principles and concepts and emphasizes throughout the...
Article
Full-text available
Roots of the Fe-efficient tomato (Lycopersicon esculentum Mill., cultivar Floradel) were cultured in an inorganic medium supplemented with glycine, thiamine, pyridoxine, and nicotinic acid, with sucrose as an energy and carbon source. Iron was supplied as ferric hydroxyethylethylenediaminetriacetic acid (FeHEDTA) and the initial PH was 5.5. Root gr...
Article
The polycyclic aromatic hydrocarbons (PAH) that contaminate soils at many industrial and government sites are resistant to natural biotic and abiotic degradation processes. The recalcitrant nature of these compounds may require aggressive chemical treatment to effectively remediate these sites. This study was conducted to assess the viability of pe...
Article
Anaerobes in the Recyling of Elements in the Biosphere.- The Diversity of Energy Sources of Microorganisms.- Mechanism of Hydrogen Activation.- Reductive Activation of Aerobically Purified Desulfovibrio vulgaris Hydrogenase: Characterization of the Catalytic H Cluster.- Iron-Sulfur Cluster Biosynthesis.- Genes and Proteins Involved in Nickel Depend...
Article
We have assessed iron acquisition by Laccaria laccata ATCC 42217 growing in defined medium with adequate levels of iron to support growth. A series of enzyme tests were conducted with mycelium to establish optimum conditions for ferric citrate reductase activity and to assess enzyme ihibition by divalent cations and by redox‐active metal oxyanions....
Article
This research was initiated to examine the influence of metal cations on ferric chelate reducing capacity of alfalfa grown in hydroponic culture under conditions of iron sufficient or iron limited conditions. In the absence of added metal ions, ferric chelate reductase was greater in plants cultivated in iron limited conditions than in plants where...
Article
As determined by transmission electron microscopy, the reduction of uranyl accetate by immobilized cells of Desulfovibrio desulfuricans results in the production of black uraninite nanocrystals precipitated outside the cell. Some nanocrystals are associated with outer membranes of the cell as revealed from cross sections of these metabolically acti...
Article
Ethylenediaminetetraacetic acid (EDTA) is a chelating agent that has been extensively used to enhance the solubilization of heavy metal cations and release of EDTA contributes to environmental problems. EDTA is recalcitrant to microbial metabolism and chemical oxidation is considered a possible method of remedial treatment. The use of the commercia...
Article
Aphanizomenon flos-aquae, a cyanobacterium that is marketed as a health food supplement, is harvested from natural blooms in Klamath Lake (Oregon) that are occasionally contaminated by Microcystis spp. Regulatory agencies in several countries are developing regulations to control the amount of microcystin in drinking water and other products, inclu...
Article
In this letter, a linear free energy relationship developed by Sverjensky and Molling is used to predict the Gibbs free energies of formation of crystalline phases of M4+O2 and M4+(OH)4 from the known thermodynamic properties of aqueous tetravalent cations (M4+). The modified Sverjensky and Molling equation for tetravalent cations is expressed as Δ...
Article
Uranium and its fission product Tc in aerobic environment will be in the forms of UO22+ and TcO4−. Reduced forms of tetravalent U and Tc are sparingly soluble. As determined by transmission electron microscopy, the reduction of uranyl acetate by immobilized cells of Desulfovibrio desuifuricans results in the production of black uraninite nanocrysta...
Article
Intact cells of Desulfovibrio desulfuricans were immobilized in polyacrylamide gel and used to remove soluble U and Mo from water by enzymatically mediated reduction reactions in column reactors. Formate or lactate served as the electron donor and oxidized U(VI) and Mo(VI) species served as electron acceptors. Greater than 99% removal efficiencies...
Article
Intact cells of Desulfovibrio desulfuricans, immobilized in polyacrylamide gel, removed Cr, Mo, Se and U from solution by enzymatic-mediated reduction reactions. Lactate or H2 served as the electron donor and the oxidized Cr(VI), Mo(VI), Se(VI) and U(VI) served as electron acceptors. Reduction of the oxidized metal species resulted in the precipita...
Article
The mobility of Mo in the environment is strongly dependent on its chemical oxidation state. Under oxidizing conditions, Mo occurs as highly soluble and mobile Mo(VI) and Mo(V) compounds. However, under reducing conditions Mo usually forms insoluble Mo(IV) phases. The objective of this study was to demonstrate the ability of the sulfate-reducing ba...
Article
In an earlier publicationCampylobacter rectuswas shown to grow with H2as the electron donor and fumarate as the electron acceptor and whole cells oxidized H2in the presence of fumarate or elemental sulfur. Relatively little is known about the metabolism ofCampylobacter rectusand our studies were to address physiological issues of H2metabolism. We r...
Article
 Previously it was demonstrated that bacteria are capable of transforming soluble uranyl ion, U(VI), to insoluble uraninite, U(IV); however, the rate for this transformation has not been determined. We report the kinetic coefficients for Desulfovibrio desulfuricans DSM 1924 grown in a continuous-flow chemostat where pyruvate was the electron donor...
Article
This research was initiated to examine the significance of the high‐affinity iron (Fe) transport system in the nitrogen (N)‐fixing activity of Rhizobium meliloti bacteroides in nodules of alfalfa, Medicago sativa L. Iron was supplied as Fe‐chelate at a concentration of 10μM for nodulation and plant growth. Nitrogen‐fixation measurements by the acet...
Article
Chromium must be removed or made immobile to reduce its toxicity in contaminated soil. The objectives of this study were (i) to compare Cr (III) and Cr (VI) sorption in three soils, (ii) to determine the potential of Cr-tolerant microorganisms to sorb Cr (III and VI) or to reduce Cr (VI) to Cr (III), and (iii) to determine the effect of organic ame...
Conference Paper
Cultures of Wolinella succinogenes and Desulfovibrio desulfuricans were grown in the presence of 10 mM selenate or 0.1 mM selenite. As the cultures grew, red elemental selenium was present in the extracellular culture fluid, indicating that selenate and selenite were transformed. The bacterial cell density was insufficient to provide an adequate qu...
Article
The mobility of uranium in groundwater is a problem of considerable magnitude. One approach would be to control the distribution of uranium by converting the water-soluble uranium ion to one that is less soluble. This study focuses on the use of Desulfovibrio gigas, D. baculatus, D. vulgaris, D. desulfuricans, Pseudomonas putida, a denitrifying Pse...
Article
Formate dehydrogenase in Desulfovibrio gigas was measured by following the release of 14CO2 from radiolabeled formate. Experiments with whole cells using sulfate as the electron acceptor revealed optimal formate dehydrogenase activity at pH 7.0 and formate utilization followed saturation kinetics. While formate dehydrogenase was constitutively prod...
Article
Full-text available
Desulfovibrio desulfuricans (DSM 1924) can be adapted to grow in the presence of 10 mM selenate or 0.1 mM selenite. This growth occurred in media containing formate as the electron donor and either fumarate or sulfate as the electron acceptor. As determined by electron microscopy with energy-dispersive X-ray analysis, selenate and selenite were red...
Chapter
Full-text available
The sulfate-reducing bacteria (SRB) are a unique physiological group of procaryotes because they have the capability of using sulfate as the final electron acceptor in respiration. Initially, these bacteria were treated as biological curiosities and little research effort was devoted to them. An appreciation of the SRB grew, in part, from an intere...
Article
Full-text available
Biosensors in the natural environment could have tremendous utility in the reduction of hazardous waste proliferation in subsurface and aquatic ecosystems. Uses directly related to waste management could be the early detection of pollutants entering the vadose zone in arid geographies and groundwater from other point sources of pollution. Microorga...
Article
This chapter discusses pyruvic acid phosphoroclastic system. Pyruvic acid is considered an important carbon source for sulfate-reducing bacteria (SRB). The bacterial metabolism of pyruvic acid produces either acetyl phosphate, H2, and CO2 or acetyl phosphate and formic acid. Reactions involving the production of acetyl phosphate from pyruvic acid a...
Chapter
Full-text available
Bacteria and other microorganisms have developed unique metabolic systems that provide resistance to toxic heavy metals. Attention is being directed to the exploitation of these microorganisms for the purpose of detoxifying metal-contaminated soil or water. This chapter reviews the emerging technology of bioremediation of metals by microorganisms a...
Article
The biofilm attributed to Desulfovibrio vulgaris growing in the presence of ferrous metals was examined with an environmental scanning electron microscope. This novel microscope produced images of iron sulfide colloids and other iron containing structures that had not been reported previously. A plaque composed of iron sulfide enveloped the surface...
Article
Pseudomonas maltophilia strain O-2, isolated from soil at a toxic waste site in Oak Ridge, Tennessee, catalyzed the transformation and precipitation of numerous toxic metal cations and oxyanions. When a viable inoculum (1%) of O-2 was introduced into nutrient broth containing Hg(II), Cr(VI), Se(IV), Pb(II), Au(III), Cd(II), Te(IV), or Ag(I), effect...
Article
Rhizobium meliloti 1021 and several Tn5 generated mutants were examined for physiological activities which would reflect the capacity of these strains for efficient iron metabolism. Rhizobactin production in liquid culture, as measured by CAS reactivity, was greatest in R. meliloti 1021 with lesser amounts formed by strains PRR 63 and PRR 62. Exami...
Chapter
Under appropriate conditions, certain bacterial strains can reduce selenate or selenite to colloidal Se and Pb II can be transformed to a Pb-containing colloid. The red Se colloids have a diameter of 250 to 350 nm while the gray-black Pb colloids are 170 to 180 nm in diameter. Like the Se colloids, Pb colloids are spherical, of uniform density and...
Article
The growth and siderophore production of a fluorescent Pseudomonas species isolated from soil contaminated with chromium was found to be influenced by the presence of trivalent cations. Overproduction of pseudobactin occurred when the isolate was grown in media containing 1 mM Cr(III) under iron-limited conditions but not when Fe(III) was added at...
Article
The growth and siderophore production of a fluorescentPseudomonas species isolated from soil contaminated with chromium was found to be influenced by the presence of trivalent cations. Overproduction of pseudobactin occurred when the isolate was grown in media containing 1 mM Cr(III) under ironlimited conditions but not when Fe(III) was added at 10...
Article
We have analyzed the ability of single site insertion mutants of Rhizobium meliloti 1021 defective in various components of a high-affinity iron transport system to produce nodules, fix nitogen and promote plant growth. Our results indicate that a high-affinity iron transport system may significantly increase the ability of the differentiated form...
Article
Full-text available
Cell extracts of Cenococcum graniforme have been found to contain the following hydrolytic enzymes: protease, esterase, alpha-d-galactopyranosidase, beta-d-galactopyranosidase, alpha-d-mannopyranosidase, beta-d-xylopyranosidase, alpha-d-glucopyranosidase, beta-d-glucopyranosidase, and alkaline phosphatase. Sulfatase, inorganic pyrophosphatase, and...