Lareb Dean

• University of Southampton

Background Airborne fine particulate matter with diameter < 2.5 μm (PM2.5), can reach the alveolar regions of the lungs, and is associated with over 4 million premature deaths per year worldwide. However, the source-specific consequences of PM2.5 exposure remain poorly understood. A major, but unregulated source is car brake wear, which exhaust emi...

Introduction: Airborne fine particulate matter (diameter <2.5μm; PM2.5) is associated with incidence and exacerbation of chronic respiratory diseases. Vehicle exhaust emissions have decreased but emissions of poorly characterised non-exhaust PM persist, the main source of which is brake wear (BWPM). However, source-specific effects of PM2.5 are poo...

Regulation of cutaneous immunity is severely compromised in inflammatory skin disease. To investigate the molecular crosstalk underpinning tolerance versus inflammation in atopic dermatitis, we utilise a human in vivo allergen challenge study, exposing atopic dermatitis patients to house dust mite. Here we analyse transcriptional programmes at the...

Introduction Airborne fine particulate matter (diameter <2.5μm; PM2.5) is a leading risk factor for ill health and premature death. Road vehicle technology is decreasing exhaust-derived PM emissions but poorly characterised, unregulated non-exhaust PM persists. This study aimed to characterise the toxicology of brakewear PM2.5 (BWPM) from 4 brakepa...

In this review, the Basic and Translational Science Assembly of the European Respiratory Society provides an overview of the 2022 International Congress highlights. We discuss the consequences of respiratory events from birth until old age regarding climate change related alterations in air quality due to pollution caused by increased ozone, pollen...

In this review the Basic and Translational Science Assembly of the European Respiratory Society (ERS) provides an overview of the 2022 international conference highlights. We discuss the consequences of respiratory events from birth until old age regarding climate change-related alterations in air quality due to pollution caused by increased ozone,...

Respiratory diseases account for over 5 million deaths yearly and are a huge burden to health-care systems worldwide. Murine models have been of paramount importance to decode human lung biology in vivo, but their genetic, anatomical, physiological and immunological differences with humans significantly hamper successful translation of research int...

Introduction: Airborne fine particulate matter (diameter <2.5μm; PM2.5) is a leading risk factor for lung disease, but differential effects of PM2.5 from different sources are poorly understood. Improvements to vehicle exhaust technology have neglected poorly characterised, unregulated “non-exhaust” PM, predominantly brakewear (BWPM). This study ai...

Atrazine (ATZ), a commonly used agricultural herbicide, is potentially harmful to animals and humans. Nanoencapsulation of ATZ (NC–ATZ) within non-toxic, biodegradable poly(ε-caprolactone) (PCL) improves the herbicidal activity of ATZ 10-fold and moderates its environmental persistence, possibly reducing off-target ecological effects. These compoun...

Extracellular matrix (ECM) stiffening with downstream activation of mechanosensitive pathways is strongly implicated in fibrosis. We previously reported that altered collagen nanoarchitecture is a key determinant of pathogenetic ECM structure-function in human fibrosis (Jones et al., 2018). Here, through human tissue, bioinformatic and ex vivo stud...

Accurate regulation of cutaneous immunity is fundamental for human health and quality of life but is severely compromised in inflammatory skin disease. To investigate the molecular crosstalk underpinning tolerance vs inflammation in human skin, we set up a human in vivo allergen challenge study, exposing patients with atopic dermatitis (AD) to hous...

Extracellular matrix (ECM) stiffening with downstream activation of mechanosensitive pathways is strongly implicated in fibrosis. We previously reported that altered collagen nanoarchitecture is a key determinant of pathogenetic ECM structure-function in human fibrosis (Jones et al., 2018). Here, through human tissue, bioinformatic and ex vivo stud...

Angiotensin-converting enzyme 2 (ACE2) is the main entry point in airway epithelial cells for SARS-CoV-2. ACE2 binding to the SARS-CoV-2 protein spike triggers viral fusion with the cell plasma membrane, resulting in viral RNA genome delivery into the host. Despite ACE2’s critical role in SARS-CoV-2 infection, full understanding of ACE2 expression,...

Angiotensin-converting enzyme 2 (ACE2) is the main entry point in the airways for 47 SARS-CoV-2. ACE2 binding to SARS-CoV-2 protein Spike triggers viral fusion with the cell membrane, resulting in viral RNA genome delivery into the host. Despite ACE2’s critical role in SARS-CoV-2 infection, an understanding of ACE2 expression, including in response...

Angiotensin-converting enzyme 2 (ACE2) is the main entry point in the airways for SARS-CoV-2. ACE2 binding to SARS-CoV-2 protein Spike triggers viral fusion with the cell membrane, resulting in viral RNA genome delivery into the host. Despite ACE2’s critical role in SARS-CoV-2 infection, an understanding of ACE2 expression, including in response to...

Background Nanomaterial inhalation represents a potential hazard for respiratory conditions such as asthma. Cerium dioxide nanoparticles (CeO2NPs) have the ability to modify disease outcome but have not been investigated for their effect on models of asthma and inflammatory lung disease. The aim of this study was to examine the impact of CeO2NPs in...