Lanyi Szabolcs

Sapientia Hungarian University of Transylvania · Department of Bioengineering

Citation: Vincze, É.-B.; Becze, A.; Salamon, R.V.; Lányi, S.; Mara, G. Role of the Pseudomonas koreensis BB2.A.1 and Serratia liquefaciens BB2.1.1 Abstract: Plant-growth-promoting rhizobacteria (PGPR), in addition to their well-known direct effects on plant growth and development, have been reported to be effective in plant abiotic (trace metal, dr...

Metabolic engineering has shown that optimizing metabolic pathways’ fluxes for industrial purposes requires a methodical approach. Accordingly, in this study, in silico metabolic modeling was utilized to characterize the lesser-known strain Basfia succiniciproducens under different environmental conditions, followed by the use of industrially relev...

In our current work we studied the expression of the Cowpea chlorotic mottle virus (CCMV) capsid protein in E. coli host cell. The CCMV proteins DNA was cloned into a vector containing a His-tag and a ubiquitin fusion partner. The gene was transformed in E. coli BL21(DE3) Rosetta cells and expressed. The expression process was optimized and shake f...

Succinic acid production through biological fermentation led to new pathways in the integration of renewable feedstock from different industries into biosynthesis. In this article, we investigate the population growth dynamics and succinic acid production potential of the recently isolated natural succinic acid producer, Basfia succiniciproducens,...

The effect of populin extract from black poplar (Populus nigra) on seven different late blight strains was tested under laboratory and field conditions. The growth rate of hyphae was found to be significantly lower in vitro after 3 and 4 v/v% populin applications. Stain M16 was resistant to populin treatment under lab conditions, however. Both 5% a...

Bio-based, environmentally benign production of commodity chemicals such as 1,4-butanediol (BDO) from renewable feedstocks is highly challenging due to the lack of natural synthesis pathways. Herein, we present a systematic model-driven evaluation of the production potential for Escherichia coli to produce BDO from renewable carbohydrates (glucose,...

Bio-based, environmentally benign production of commodity chemicals such as 1,4-butanediol (BDO) from renewable feedstocks is highly challenging due to the lack of natural synthesis pathways. Herein, we present a systematic model-driven evaluation of the production potential for Escherichia coli to produce BDO from renewable carbohydrates (glucose,...

1,4-butanediol (BDO) is an important commodity molecule that is used as a platform chemical for the production of polybutylene terephthalate (PBT), elastic fibres (Spandex) and other materials. The homologous enzyme of E. coli, succinyl-CoA synthetase (sucCD) and the heterologous malonyl-CoA reductase from Chloroflexus aurantiacus (mcr) are key enz...

A part of rhizospheric bacteria are considered plant growth-promoting bacteria (PGPB) due to their positive effect on the plant growth and development. Plant growth-promoting bacteria based on their metabolic activity can be grouped as biofertilizers, fitostimulants, or biopesticides. These efficient bacteria due to various direct or indirect effec...

Development of systems biology, genome engineering and metabolic engineering tools enabled the biosynthesis of chemicals from sustainable raw materials: industrial-scale processes include microbial production of succinic acid, lactic acid, amino acids and other metabolites [1,2]. Biology-based production of 1,4-butanediol is one of the industry's m...

This paper presents the evolution of ozone concentration for the 2007-2016 decade and a comparison with key values related to human and vegetation health. As temperature is one of the main factors influencing ozone concentration in this area, the most significant changes of air temperature and extreme temperature indices for the 2007 - 2016 decade...

According to EU Strategy for the Baltic Sea Region, Lithuania obligates to ensure sustainable growth, gain and maintain good condition of marine environment until 2020. In accordance with the sustainability approach, every potential cost and energy cutting as well as social sustainability measure for wastewater treatment should be explored. Nonethe...

Recent developments in the field of metabolic engineering made possible production of high added-value chemicals form sustainable raw materials: as examples of industrial applications microbial production of succinic acid, lactic acid, amino acids and other metabolites can be mentioned. Bio-technological production of 1,4-butanediol represents a ma...

Present work highlights cloning, heterologous expression, purification and chemical labelling of proteomimetic SMAC peptides. Genes of active part (AVPI binding motif) of Second Mitochondria-Derived Activator of Caspases (SMAC) protein was cloned into pET-19b ubiquitin fusion partner containing expression vector. Successful shake flask and large-sc...

Bio-succinateproducedbymicroorganismisavaluableplatformchemical,whileBasfiasucciniciproducens,asarecentlydescribednaturalsuccinicacidproducerisapromisinghostforapplicationinbiotechnologies,andwiththedevelopinggenomeengineeringmethodsofthisstrainitsapplicationspectrumisextending.HerewereportastudyregardinginvestigationoffermentationconditionsforB.su...

New proteins as molecular targets in development of therapies are discovered every day. However, study of their interactions with other proteins or binding partners in complex cellular environments has its limits. Therefore, high-yield production of these proteins in heterologous systems is a valid necessity, while obtaining these proteins linked t...

Recently, several approaches have been published in order to develop a functional biosynthesis route for the non-natural compound 1,4-butanediol (BDO) in E. coli using glucose as a sole carbon source or starting from xylose. Among these studies, there was reported as high as 18 g/L product concentration achieved by industrial strains, however BDO p...

Studia Universitatis Babes Bolyai Seria Chemica, 55, Special Issue (2010): 81-88.

The main aim of this study was to create mutant strains with lambda Red recombination system from an Escherichia coli strain which was isolated from animal faeces. E. coli Ter8/1 strain was genetically modified using lambda Red recombination system and two mutant strains were obtained, TerP01, in which the gene of pyruvate formate lyase was inactiv...

A total number of 246 lactic acid bacteria were isolated from naturally fermented traditional foods and feed. These isolates were phenotypically characterized, classified and identified using 16S ribosomal DNA sequencing. 13 different species were detected from cheeses and from fermented plant materials. The isolates belonged to four genera: Lactob...

The histidine-modified EGFP was characterized as a sensing element that preferentially binds nanomolar concentrations of Cu2+ in a reversible manner (Kd = 15 nM). This research aims to determine the causes of nanomolar-affinity of this mutant by investigating significant structural and energetic alterations of the chromophore in the presence of dif...

This work investigates the effect of technical parameters on the anaerobic digestion of the corn-DDGS in laboratory-scale bioreactors under mesophilic conditions. The experimental results show that both pH-control and stirring have a significant influence on the methane production. With respect to the conditions without stirring and without pH-cont...

Naturally occurring and anthropogenic petroleum hydrocarbons are potential carbon sources for many bacteria. The AlkB-related alkane hydroxylases, which are integral membrane non-heme iron enzymes, play a key role in the microbial degradation of many of these hydrocarbons. Several members of the genus Rhodococcus are well-known alkane degraders and...

The bio-based conversion of glycerol and glucose to valuable chemicals, such as succinate, by commonly used microorganisms, like Escherichia coli is of major interest. The petrochemically based succinate production can be replaced by a new sustainable and environmental friendly one and the glycerol resulted from biodiesel industry can be therefore...

This study investigated the individual and interactive effects of three factors — temperature, inoculum/substrate ratio (ISR) and inoculum typology — on the anaerobic digestion of corn ethanol distillery wastewater. Biochemical methane potential assays planned with factorial design with two independent quantitative variables on three levels (ISR: 1...

The low level of selenium in soils from different Romanian areas indicates a need for its supplementation. Biofortification through treatments applied to crops has the advantage of supplementation of food chain with controlled levels of selenium compounds, with high bioavailability. We present a biotechnological approach of biofortification, adapte...

Succinic acid is currently used in the agricultural, food and pharmaceutical industries, is one of the key basic chemicals used in the preparation of biodegradable polymers. The first step in the fermentative production of succinic acid is the screening of bacterial strains. Therefore we were isolated a number of 65 E. coli strains from different a...

Indole-3-acetic acid (auxin) is a well known phytohormone which is involved in the regulation of plant growth and development. A high number of plant growth promoting rhizobacteria (PGPR) produce auxin, thus play major role in plant development. In this study we characterized 25 bacterial strains by their auxin producing ability. The growth promoti...

Our society is merely based on petroleum, however its availability and the impact on environment makes this energy source unsustainable. Hence, search for new renewable energy sources is of major interest. Biodiesel as biofuel is a promising source and intensively studied for the substitution of fossil fuels. However, during the production process...

Industrial biotechnology is an important tool for the production of many commodity and specialty chemicals using microorganisms. Many microorganisms are able to produce industrially important organic compounds, but there are some barriers in the economical production. Metabolic engineering can be used to overcome these problems. The λ-Red recombina...

Succinic acid is identified to have a great economical potential in a biobased economy. To improve the production in Escherichia coli metabolic engineering should be carried out. For new mutant strain design is important to in silico simulate and analyze the metabolic changes, network of a cell under different environmental and/or genetic perturbat...

BACKGROUND The aim of this study was to investigate biopolymers and extracellular enzymes in whole activated sludge flocs originating from a full-scale wastewater treatment plant, by means of confocal laser scanning microscopy (CLSM) techniques. Both sectioned and whole activated sludge floc samples have been stained using specific fluorochromes an...

Two major problems of the rapidly expanding corn bioethanol industry are the fate of the massive by-product amounts, and the high energy demand of by-product processing. Traditional by-product processing methods consist mostly of the evaporation and drying of distillery wastewater, hence they are very energy-intensive, worsening the already modest...

A mutant strain of the anaerobic purple sulfur bacterium Thiocapsa roseopersicina, containing only nitrogenase as a functionally active enzyme for H2 generation was utilized to study the production of H2 from organic acids (acetate, pyruvate and succinate). Two types of potential substrates for H2 production, thiosulfate and salts of various organi...

Nowadays, because of substantial use of petroleum-derived fuels the number and extension of hydrocarbon polluted terrestrial ecosystems is in growth worldwide. In remediation of aforementioned sites bioremediation still tends to be an innovative, environmentally attractive technology. Although huge amount of information is available concerning the...

The glucocorticoid-induced tumor necrosis factor receptor (GITR) is a member of the tumor necrosis factor receptor superfamily. Attachment of GITR to its ligand (GITRL) regulates diverse biological functions, including cell proliferation, differentiation, and survival. In this study, the extracellular region of human GITRL (hGITRL) was cloned, expr...

The thermophilic and mesophilic bacteria are a rich source of pullulan degrading enzymes. The gene encoding pullulanase enzyme from the thermophilic Fervidobacterium pennivorans was cloned, and its heterologous expression in E. coli was achieved. The pullulanase gene encoding 544 amino acids has overexpressed under the control of a strong promoter....

Transferrin-iron(III)-anion complexes internalization and cytotoxic effects were determinedonglioblastoma cells. The Tf-complexes cellular uptake was followed by fluorescent labelling of the protein. Cellular incorporation testshows the highest efficacy of transferrin-iron-bicarbonatecomplex. The induced cell membrane demageand the decrease of mito...

a b s t r a c t Plant growth promoting bacteria can enhance and promote plant growth and development in different ways. These mechanisms include solubilization of phosphorus, nitrogen fixation and biocontrolling effects on phytopathogenic microorganisms. Suppression of phytopathogenic fungi by 47 different bacteria, isolated from different monocoty...

The main aim of our study is the development of bacterial biopreparates based on organic nitrogen and phosphorus mobilizing microorganisms. In this study we analyzed the nutrient mobilizing ability (cellulase, phytase, lecithinase, alkaline phosphatase and alkaline protease enzyme activities) from organic material of the soil bacteria isolated from...

The nitrogen and phosphorus management in agricultural systems leads to an increased input of plant nutrients and sustains the soil fertility. Microbes contribute through different mechanisms to nutrient cycles, transforming it available for plants. Isolation of 18 Azospirillum and 18 Azotobacter bacterial isolates was effectuated from the rizosphe...

In this study it was realized an efficient refolding of the human GITRL produced in E. coli as inclusion bodies utilizing different recombinant vectors (pET20b-hGITRL, pETM52-hGITRL). The insoluble proteins were solubilized in guanidine hydrochloride and then were refolded by rapid dilution into a red-ox buffer composed of GSSG-GSH. Efficiency of t...

Glicoproteina transferina serică umană (Tf) are două loburi. Fiecare lob are un situs de legare a ionilor de fier trivalent. În urma legării ionilor de metale şi a diferitelor anioni (oxalat, aziridin-carboxilat, bicarbonat) la acest situs se pot obţine patru forme moleculare a transferinei (holo-Tf, diferri Fe N TfFe C , şi monoferri TfFe N , TfFe...

Uscarea prin pulverizare este o metodă frecvent folosită la încapsularea microorganismelor probiotice. În prezentul studiu a fost utilizat duză ultrasonică de 120 kHz pentru a obţine granule foarte fine, care pot fi folosite în produse lactate probiotice. Celule de bacterii lactice au fost încapsulate în alginat şi maltodextrină prin atomizare cu u...

Granzyme H belongs to the family of serine proteinases, which plays an important role as part of the natural immune system. This enzyme is produced by natural killer cells and the T lymphocytes, inducing apoptosis in target cells. The precise role of the enzyme is not yet clarified, its crystal structure is unknown. Granzyme H was cloned by RT-PCR...

The metal sensitivity and selectivity of two fluorescent proteins was investigated. The studied proteins are mutant forms of the red fluorescent protein (DsRed); according to our results, both proteins showed sensitivity to the presence of copper[II] and nickel ions. The fluorescence intensity of the proteins has decreased significantly in the pres...

Since bioremediation is an environmentally friendly, cost effective approach to treat hydrocarbon-contaminated soils, in this study our aim was to develop a bacterial soil inoculant applicable in elimination of aliphatic, simpleand polyaromatic hydrocarbons from soils. To reach our goal two bacterial strains, Pseudomonas fluorescens BBN1 and Rhodoc...

More of the newest developments in volatile oils extraction from vegetal materials by hydro distillation (HD) are represented by the different variants with microwave heating of the samples. In this paper the microwave assisted hydrodistillation (MWHD) was compared with conventional technique, hydrodistillation (HD), in order to characterize the ki...

Human serum transferrin has a potential for drug-delivery systems. Oxalate and aziridine-carboxylate was conjugated to serum transferrin in order to transport into the targeted cancer cells via transferrin-receptor mediated endocytosis. Capillary zone electrophoresis and capillary isoelectric focusing were used to analyze the effectiveness of compl...

The aim of this work is the study of activated sludge floc samples stained with different dyes and imaged by Confocal Laser Scanning Microscopy (CLSM). Activated sludge flocs were stained for lipids, sugars and esterase enzyme producing bacteria. Images were acquired for different samples to analyze the structural characteristics of the bioaggregat...

Most of the bacteria present in the rhizosphere establish a beneficial relationship with the plants in their environment. The growth-promoting abilities of two bacterial strains on seed germination and seedling growth and development was assayed in corn (Zea mays), pea (Pisum sativum) and bean (Phaseolus vulgaris) under laboratory conditions. The s...

Due to antigen recognition, effector and regulatory function, lymphocytes have pivotal function in many disease pathways. Immune response towards foreign or altered self antigens has a considerable complexity, the fine modulation of the immune response being delivered by the interaction between costimulatory molecules, on which the final outcome of...

Indigenous hydrocarbon-degrading bacteria play an important role in natural attenuation process of hydrocarbon contaminated soils. Through enzyme systems they catalyze the mineralization of contaminants into non toxic forms, into CO2 and H2O. In order to assess the intrinsic bioremediability is essential to know the microbial community structure, a...

ABSTRACT. Granzyme H belongs to the family of serin proteases, which plays an important role in fighting infections as part of the natural immune system. This enzyme is produced by natural killer cells and the T lymphocytes, inducing apoptosis in target cells. The precise role of the enzyme is not yet clarified; its crystal structure is unknown. Th...

The aim of our study was the isolation and screening of plant and soil bacteria with multiple beneficial effects on plants in order to contribute to the application of these isolates as biofertilizers, an alternative strategy to avoid the application in excess of mineral fertilizers. The beneficial microbes play an important role in the transformat...

Borsáros is a nutrient poor oligotrophic raised Bog, where organic materials accumulate and are stored. Microorganisms play important role in the degradation processes, thus contributing to the local circulation of nutrients1. The aim of our study is to analyze Bryophyte associated bacterial strains isolated from Borsáros raised bog. Plant growth p...

The aim of the present study was to describe the beneficial health effects of probiotic bacteria, manifested by inhibition of growth of pathogen microorganism strains and that of their colonization of the gastrointestinal tract of humans. The primordial mode of action by which a probiotic eradicates a pathogen can be facilitated by the production o...

In the course of the research we determined selenium and dry-matter content of 35 wheat grasses and 35 wheat seeds. The selenium content of the preparation plant samples was measured by spectrofluorimetric determination (ʎexcitation=380 nm, ʎemission=519 nm) of the resulted piaz-selenol complex. It was established that between the selenium content...

This study presents the result of a screening of symbiotic nitrogen fixing bacteria from a specific mountainous grassland habitat lying at 1200 m above sea level in the Ciuc Mountains. A number of 50 bacterial isolates from rhizosphere and from the nodules of 14 specific leguminous plant species are presented and characterized. Pure cultures were o...