Lamberto Re

Lamberto Re
Marche Polytechnic University | Università degli Studi di Ancona · Department of Life and Environmental Sciences - DiSVA

M.D.

About

114
Publications
49,163
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Introduction
Lamberto Re worked at the Department of Life and Environmental Sciences - Pharmacology, Università Politecnica delle Marche. Lamberto does research in Didactics and Public Health. Their current project is 'Ozone Research' at Medinat Clinic.
Additional affiliations
January 1995 - present
Medinat Sas
Position
  • Scientific Director
Description
  • Supervision and Protocols of Ozone Treatment and Supplementation
Education
October 1974 - July 1983
Marche Polytechnic University
Field of study
  • Medicine

Publications

Publications (114)
Article
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Currently, treatment with medical ozone (MO) is considered one of the most interesting and safe integrative options that can effectively complement many conventional medical therapies, mainly, but not exclusively, involving aging and pain [...]
Chapter
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Una panoramica sulla letteratura riguardante l'utilizzo dell'ozonoterapia in Medina Interna
Article
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Notwithstanding the use of ozone in medicine has become widespread in many countries of the world, its real pharmacological action remains not completely clarified. We know that other than its uses as disinfectant, well documented by the literature since the beginning of the past century, the more recent medical use of ozone in several pathologies...
Article
Full-text available
Notwithstanding the use of ozone in medicine has become widespread in many countries of the world, its real pharmacological action remains not completely clarified. We know that other than its uses as disinfectant, well documented by the literature since the beginning of the past century, the more recent medical use of ozone in several pathologies...
Article
Full-text available
Ozone is not nowadays used in a single medical branch, due to its immune and metabolic effects. This molecule is considered as a pro-drug which can induce at certain non-toxic doses a rearrangement of the biochemical pathways with the activation of a second messenger in a cascade with a multiple system action. Nonetheless, its difference from a dru...
Article
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Despite the fact that levodopa has proven its effectiveness in treating the symptoms of Parkinson's disease (PD), increasing concerns have emerged about its possible long-term toxic effects on dopamine (DA) neurons. The study investigated the possible ameliorative effect of virgin coconut oil against L-dopa-induced neurotoxicity in adult rats. A to...
Article
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Despite various opinions and healthy controversy on Ozone Therapy (OT), the practices of this therapy have increased worldwide. Main areas of study with consistent scientific outcomes are the topical treatment of both disk herniation and periodontal disease. On the other hand, there is a net dissociation of the scientific resonance concerning syste...
Article
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The aim of the multicentre study promoted by Nuova FIO is to evaluate the beneficial effects of the systemic Oxygen-Ozone (O2O3) therapy in patients suffering from SARS COV-2 disease in the early phases of the disease, before worsening, up to the need of tracheal intubation. The study is based on the rationale on that the systemic oxygen-ozone trea...
Article
Full-text available
Ozone therapy is widely used in many countries since many years. Recently, the increasing widespread of this complementary therapy has been accomplished by an increased number of basic and clinic papers published on international journals. This lecture will deal on the first approach by a pharmacological point of view in the aim to characterize the...
Article
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In recent years, emphasis and attention have been focused on the use of medical ozone (O3). Despite its huge diffusion, certain confusion still persists concerning its potential toxicity as a strong oxidant. Although its use as safety remedy in the medical field has been described since the last century, this confusion still represents a major fact...
Article
Background: No satisfactory treatment exists for chronic rejection (CR) after lung transplantation (LT). Our objective was to assess whether ozone (O3) treatment could ameliorate CR. Methods: Male Sprague-Dawley inbred rats (n = 36) were randomly assigned into four groups: (1) control (n = 6), (2) sham (n = 6), (3) LT (n = 12), and (4) O3-LT (n =...
Article
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Received: December 21, 2015 Accepted: December 21, 2015 Published online: February 27, 2016 Issue release date: May 2016
Article
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Ozone therapy is widely used in many countries since many years. Recently, the increasing widespread of this complementary therapy has been accomplished by an increased number of basic and clinic papers published on international journals. Many of the basic mechanisms of the ozone action are now well outlined. In addition, the modulation of interle...
Article
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Atherosclerosis is a major cause of death in the Western World. It is known that Lipofundin 20% induces atherosclerotic lesions, while ozone at low doses has been satisfactorily used in the prevention of oxidative stress-associated pathologies, such as coronary artery diseases. The aim of the present work was to evaluate the effects of ozone therap...
Article
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Coronary artery disease (CAD) is the most common cause of sudden death, and death of people over 20 years of age. Because ozone therapy can activate the antioxidant system and improve blood circulation and oxygen delivery to tissue, the aim of this study was to investigate the therapeutic efficacy of ozone in patients with CAD, treated with antithr...
Article
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The aim of the present review is to clarify some of the basic mechanisms underlying ozone therapy. Indeed, after its empiric use started at the beginning of the last century, science is now ready to give a chance to the more and more medical doctors working in this field. Unfortunately, the lack of a full recognition by the health authorities and s...
Article
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Resumen Las primeras evidencias sobre el uso clínico de los aceites ozonizados aparecen en la literatura científica desde 1859. Para la realización de este trabajo se tuvieron en cuenta los aspectos generales y las principales aplicaciones clínicas de los aceites ozonizados que aparecen en las base de datos entre 1859 y 2011. Los productos de la ox...
Article
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Controlled ozone administration has been shown to promote an adaptation to oxidative stress by increasing endogenous antioxidant systems. The present study investigated the effects of O2-O3 administration either prophylactically or therapeutically on the alterations of oxidant status in adjuvant-induced arthritis in rats. Seven groups of rats were...
Article
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The rectal administration of ozone is one of the oldest systemic and local forms of application. The biological effects of the Rectal Insufflations of Ozone (RIO3) has been demonstrated extensively either experimentally or clinically. Furthermore, preclinical studies demonstrated its low toxicity. RIO3 has been now extended to treat many diseases a...
Article
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Coronary artery disease is considered a major cause of death in the western world and its primary pathological manifestation is myocardial damage due to ischemia-reperfusion phenomena. Ozone has been used as a therapeutic agent and beneficial effects against the damage induced by renal or hepatic ischemia-reperfusion have been observed in vivo. The...
Article
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Redox environment involves a broad network of pro-oxidant and antioxidant components. Health benefit or damage can be induced as a consequence of an adaptive cellular stress response. A consequence of hormetic amplification is an increase in the homeodynamic space of a living system in terms of an increased defense capacity and a reduced load of da...
Article
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To address the clinical evidence that may support the fact that subcutaneous administration of ozone (O₃) has anti-hyperalgesic effects probably acting by modifying specific pain targets. Fifty-two patients attending the Medinat Clinic, Camerano, Ancona, Italy between October 2004 to October 2009 were eligible to participate in the study. Panoramic...
Article
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Basic fibroblast growth factor (bFGF) is a pleiotropic mitogen which plays an important role in cell growth, differentiation, migration and survival in different cells and organ systems. Application of bFGF has been shown to promote cellular proliferation and collagen synthesis in vivo. FGF is markedly up-regulated following bone or tendon injury a...
Article
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The in vivo genotoxic effect of ozone/oxygen mix (O2-O 3) was studied in leukocytes and exfoliated colorectal cells of SD rats using the Comet assay (single cell gel electrophoresis assay, SCGE). O 2-O3 (O3 final dose 42 g/kg b.w.) was applied for four days by colorectal insufflation simulating human ozone therapy. Bleomycin (15 mg/kg b.w., i.p.) w...
Article
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Hypertensive disorders are one of the commonest medical diseases affecting pregnancy. The present study determined the effect of ozone therapy, in the form of rectal insufflations on foetoplacental blood flow in hypertensive pregnant women. Adult volunteer (30) primigravidae and multipara hyper-tensive pregnant women at 24 weeks gestation were grou...
Article
Aflatoxins are highly hazardous contaminants of common food and feed. Aflatoxin B1 in particular, the most predominant among aflatoxins, was thoroughly demonstrated to be highly toxic, mutagenic, teratogenic and carcinogenic in many animal species. Besides its established targets and effects, this work investigates on the possible direct interactio...
Article
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-Mitochondria-produced reactive oxygen species (ROS) are thought to contribute to cell death caused by a multitude of pathological conditions. However, a low physiologically relevant concentration of reactive oxygen species could be useful and essential to regulate a variety of key molecular mechanisms. Rotenone exposure has been reported to produc...
Article
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-The efficacy of topical OLEOZON ® against tinea pedis has already been demonstrated. The aim of the present study was to assess the adverse reactions associated with topical OLEOZON ® in pa-tients with tinea pedis. A multicenter, open, phase IV clinical trial was carried out. An adverse drug reaction report form specifying the reactions most commo...
Article
Full-text available
Ozone therapy has been widely used in many countries for many years and has recently continued to spread in the wake of an increasing number of basic and clinical papers published in international journals. Many of the basic mechanisms of ozone action are now well outlined. In addition, the modulation of interleukin productions and of some biochemi...
Article
Full-text available
Ozone has recently been subjected to criticism and emphasis in relation to clinical efficacy and toxicity, respectively. Without a doubt, ozone, in common with oxygen itself, is one of the most potent oxidants. Ozone is considered one of the major pollutants in urban areas. Nevertheless, increasingly widespread use lately has highlighted the potent...
Article
The liver is damaged by sustained ischemia in liver transplantation, and the reperfusion after ischemia results in further functional impairment. Ozone oxidative preconditioning (OzoneOP) protected the liver against ischemia/reperfusion (I/R) injury. The aim of this study was to investigate the role of A(1) adenosine receptor on the protective acti...
Article
Full-text available
Two presynaptic phospholipases A(2) (PLA(2)), neuwieditoxin-I (NeuTX- I) and neuwieditoxin-II ( NeuTX-II), were isolated from the venom of Bothrops neuwiedi pauloensis (BNP). The venom was fractionated using molecular exclusion HPLC (Protein-Pak 300SW column), followed by reverse phase HPLC (mu Bondapak C18 column). Tricine-SDS-PAGE in the presence...
Article
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Evidence indicates that oxidative stress refers to a condition where cells are subjected to excessive levels of reactive oxygen species (ROS). Overall vascular function is dependent upon a fine balance between oxidant and antioxidant mechanisms which is required, at least in part, for proper functioning of the endothelium. Considerable experimental...
Article
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During the process of energy production in aerobic respiration, vascular cells produce reactive oxygen species (ROS). A growing body of evidence indicates that oxidative stress refers to a condition in which cells are subjected to excessive levels of ROS. Overall vascular function is dependent upon a fine balance of oxidant and antioxidant mechanis...
Article
Cellular prion protein (PrP(c)), the normal isoform of the pathogenic peptide (PrP(sc)) responsible of the transmissible spongiform encephalopaties (TSEs), is present in many neural tissues, including neuromuscular junctions (NMJ). To analyze if this protein could influence the synaptic transmission, we performed an electrophysiological approach to...
Article
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Oxidative stress is suggested to have an important role in the development of complications in diabetes. Because ozone therapy can activate the antioxidant system, influencing the level of glycemia and some markers of endothelial cell damage, the aim of this study was to investigate the therapeutic efficacy of ozone in the treatment of patients wit...
Article
The liver is damaged by sustained ischaemia during liver transplantation, and the reperfusion after ischaemia results in further functional impairment. Ozone oxidative preconditioning (OzoneOP) protected the liver against ischaemia/reperfusion (I/R) injury through different mechanisms. The aim of this study was to investigate the influence of the i...
Article
Full-text available
The application of oxygen ozone therapy in muscle and joint injuries has been neglected in the past both for the presence of consolidated pharmacological principles like FANS, corticosteroids, and for the presence of instrumental and rehabilitative techniques endowed with a certain and safe effect. Our experience with ozone derives from different b...
Article
Many studies indicate that oxygen free-radical formation after reoxygenation of liver may initiate the cascade of hepatocellular injury. It has been demonstrated that controlled ozone administration may promote an oxidative preconditioning or adaptation to oxidative stress, preventing the damage induced by reactive oxygen species and protecting aga...
Article
The St. John's Wort (Hypericum perforatum) extract (Hp) represents one of the most useful natural therapeutic agents in the treatment of moderate and mild depression. The antidepressant effects of Hp are different, by a molecular mechanism point of view, when compared to those of other antidepressant drugs and, we think, a further pharmacological c...
Article
It has been demonstrated that ozone, probably through a mechanism of oxidative preconditioning (OP), protected the liver against the damage mediated by reactive oxygen species. Taking into account that iscehmic preconditioning (IschP) is also a protective mechanism, a comparative study between both preconditioning settings was performed in order to...
Article
The effect of Mangifera indica L. extract (Vimang) on treatment of injury associated with hepatic ischaemia/reperfusion was tested. Vimang protects from the oxidative damage induced by oxygen-based free radicals as shown in several in vitro test systems conducted. The ability of Vimang to reduce liver damage was investigated in rats undergoing righ...
Article
Many studies indicate that oxygen free-radical formation after reoxygenation of liver may initiate the cascade of hepatocellular injury. It has been demonstrated that controlled ozone administration may promote an oxidative preconditioning or adaptation to oxidative stress, preventing the damage induced by reactive oxygen species (ROS) and protecti...
Article
Ozone has been used as a therapeutical agent and beneficial effects have been observed. However so far only a few biochemical and pharmacodynamic mechanisms have been elucidated. We demonstrate that controlled ozone administration may promote an oxidative preconditioning or adaptation to oxidative stress, preventing the damage induced by reactive o...
Article
We compared the protective abilities of Mangifera indica L. stem bark extract (Vimang) 50-250 mgkg(-1), mangiferin 50 mgkg(-1), vitamin C 100 mgkg(-1), vitamin E 100 mgkg(-1)and beta -carotene 50 mgkg(-1)against the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced oxidative damage in serum, liver, brain as well as in the hyper-production of react...
Article
Full-text available
Linalool is a monoterpene compound reported to be a major component of essential oils in various aromatic species. Several linalool-producing species are used in traditional medical systems. Among these is Aeolanthus suaveolens G. Dom (Labiatae) which is used as an anticonvulsant in the Brazilian Amazon. Psychopharmacological in vivo evaluation of...
Article
Natural extracts have been proved to be useful in different human pathological conditions. The scientific consideration of the therapeutic potential of plant extracts is still inappropriate due to the lack of both pharmacological and epidemiological basic studies. Here, we started from an electrophysiological point of view, a study on the effects o...
Article
The use of a mixture of low concentrations of ozone (O3) with oxygen (O2) have been proved to be useful in different human pathological conditions. Owing to a lack of both pharmacological and epidemiological basic studies, the scientific consideration of this therapeutic potential is still inappropriate. Here, we started, from an electrophysiologic...
Article
The existence of a modulatory system controlling the acetylcholine (ACh) release was first proposed for the nicotinic subtype in 1962. Following the first observation of a possible positive feedback loop activated by the released Ach, many studies were oriented in the investigation of the involved presynaptic autoreceptors. Most of the data have be...
Article
Full-text available
Glycine was applied at a range of different concentrations to test possible effects at the neuromuscular junction of the mouse. The presynaptic control of acetylcholine (ACh) release and the postsynaptic activation of the nicotinic receptor have been analysed by means of extracellular recording with an EPC7 Patch Clamp amplifier. The results indica...
Article
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Analytical and exploratory in vitro, in situ and in vivo, physio-pharmacotoxicology, from enzymology to population epidemiology, now embraces those approaches that correlate complex dynamic multisubstrate kinetics through conventional and more recent non-invasive quantitative methodologies. Basically, substrates may be classed as pertaining to fund...
Article
Functional studies were performed to evaluate the effects of methoctramine at the neuromuscular junction of the mouse. The presynaptic control of acetylcholine release and the postsynaptic activation of the nicotinic receptor have been analysed by means of the extracellular recording with an EPC7 Patch Clamp amplifier. This electrophysiological met...
Article
1. Functional studies were performed to evaluate the effects of some muscarinic agents at the neuromuscular junction of the mouse. 2. The presynaptic control of acetylcholine release and the postsynaptic activation of the nicotinic receptor have been analyzed by means of extracellular recording. The amplitude of spontaneous and of evoked acetylchol...
Article
Xenopus laevis heart was studied by 31P NMR using a 200 MHz proton spectrometer; hearts were perfused, at pH 7.35 and room temperature, with normal oxygenated or K(+)-enriched Ringer. Solution was later added with creatinol-O-phosphate (COP), phosphocreatine (PCr), adenosine triphosphate (ATP), fructose-1,6-diphosphate (FDP) and ouabain. NMR spectr...
Conference Paper
Since the QT interval changes with time under the influence of heart rate and autonomic input, it needs to be analyzed on a long-term basis. The authors present a method for automatic QT measurement based on linear regression analysis, by which the end of the T wave is determined as the crossing point between the baseline and the tangent to the las...
Article
Some techniques to block muscular nerve evoked contraction involve pharmacological approaches using synaptic blocking agents. Such methods interfere with normal synaptic transmission, and could introduce artifacts making difficult the experimental interpretation. The method based on the use of formamide pre-treatment should not interfere with synap...
Article
Full-text available
The low energy laser irradiation (LELI) has been reported to affect excitability in living tissues. In order to study the possible modification induced at the molecular level, we employed an electrophysiological technique on an in vitro mouse diaphragm preparation. The analysis was carried out either on physiological or in KCl stressed preparation....
Article
Skin extracts of the Australian frog Pseudophryne coriacea (PsC) potentiate and prolong the bioelectric activity of various excitable tissues. When studied by electrophysiological means in a preparation of mouse diaphragm, PsC skin extracts did not affect the spontaneous acetylcholine (ACh) release. However, the indirect stimulation of the preparat...
Article
Spontaneous fluctuations in heart rate were analyzed by spectral analysis in the ether anesthetized and pithed adult rat. In order to investigate the changes in the spectral pattern of the fluctuations, the selective 5HT-2 and -3 isoreceptor blockers ritanserin and BRL 43694A were used. In both the experimental conditions, ritanserin blockade led t...
Article
The mouse diaphragm muscle fiber was studied using the loose patch clamp technique. The voltage gated sodium currents were evoked by step pulses from a holding potential of about - 70 mV. Following the activation of the sodium current, a very large and fast outward current was evoked. The sensitivity of this current to 4-aminopyridine and tetraethy...
Article
The endothelium-dependent acetylcholine, metacholine, carbachol, betanechol, and furtrethonium relaxation values have been measured in vitro for the rabbit aorta, in presence of a high concentration of hexamethonium. The produced EPMR values complete the values that were obtained in a previous experiment under analogous conditions for rat bladder,...
Article
A new and automated on-line analysis of the parameters related to the neuromuscular activity is shown. The procedure used to obtain in real time the statistical evaluation of the recorded electrical signals is presented and discussed. The hardware is composed of a PDP-11/73 microcomputer equipped with an analog input/output board. The software is w...
Article
Zipeprol, a piperazine ethanol derivative, is a non-essential but widely used (paediatric) antitussive, which is not legally considered as being capable of creating dependence or abuse liability. A first group of experimental results was obtained assaying the displacement of 1 nM [3H]naloxone by zipeprol vs morphine on the rat brain homogenate frac...
Article
The effects of some histamine H2-receptor antagonists on the cholinergic system have been evaluated at the mouse end-plate. Previous data revealed interactions at both pre- and postsynaptic sites for cimetidine and ranitidine. The present work shows the effects of the same two drugs, and of famotidine, on some parameters related to the neuromuscula...
Article
The sartorius muscle of Rana esculenta was fixed and dehydrated by different methods and cross-sections of sarcomeres of different lengths were examined by electron microscopy. The area within the outlines of the thin filaments in negatives was measured using a high-resolution television system and a graphics tablet or by using a microdensitometer...