La-ongthong Vajrabhaya

Rangsit University Pathumthani,Thailand · Endodontic Section

Backgound/Objectives: This study investigated the efficacy of a herbal toothpaste containing Aloe vera (test group) compared with a sodium bicarbonate toothpaste (active control group) and a standard toothpaste (benchmark group) on periodontitis treatment outcomes. Methods: Fifty-four periodontitis patients were randomly allocated into three groups...

Background While professional dental treatments are mandatory for advance periodontitis, maintaining proper oral hygiene can significantly contribute to preventing disease progression. Some herbal toothpastes and natural plant components may have some beneficial properties. Aloe vera is a medicinal plant with active ingredients that show antioxidan...

A bstract Aim To determine the effect of an herbal toothpaste containing Little Ironweed and Java Tea, on reducing dentine permeability in vitro . Materials and Methods Dentine discs from human mandibular third molars were divided into three groups and brushed with herbal toothpaste, nonherbal toothpaste, or deionized water. Each group was immers...

The purpose of this study was to evaluate the clinical used of resin-modified glass ionomer; Embaze produced by Research Unit Herbal Medicine Biomaterial and Material for Dental Treatment, Chulalongkorn University compared to an imported commercial resin modified glass ionomer; VitrebondTM. Twenty volunteers with age range between 19-62 years old p...

Background Prevention is a preliminary focus of periodontitis treatment. Rather than giving complicated treatment to a periodontitis patient, a variety of toothpastes have been suggested to prevent periodontal disease progression. Herbal toothpastes containing natural plant components for maintaining or increasing healing might be a treatment modal...

Objectives To determine the effect of various horizontal beam angulations on radiographically separating superimposed canals in upper premolars with different external root morphologies. Materials and methods The independent variables were 1) three different external root morphologies of upper premolars (n = 30); one-root (A), fused-root (B) and t...

Abstract Background Various assays are used to evaluate the cytotoxic effect of chemicals on cultured cells. The sulforhodamine B (SRB) colorimetric assay is based on the ability of the SRB dye to bind basic amino acid residues on proteins. In contrast, the MTT (dimethylthiazol-diphenyltetrazolium bromide) colorimetric assay is based on mitochondri...

Objective To evaluate the cytotoxic and the proliferative effect of cuttlefish bone on MC3T3-E1 osteoblast cell line. Materials and Methods MC3T3-E1 cells were treated with 0.5, 1, 5, 25, 50, 100, or 200 μg/ml cuttlefish bone powder (CBP). Cytotoxicity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. This...

Objectives This is an in vitro study to develop a formulation of a hypochlorite solution for root canal irrigation that lacks a chlorinated odor. The antibacterial effect, tissue dissolution efficacy, and the cytotoxicity of the solution were assessed in cell culture and were compared with those of commercial sodium hypochlorite (NaOCl) solutions....

Objectives: The aim of this study was to evaluate dentine permeability after brushing with Twin Lotus(®), Thai herbal toothpaste by comparing with Sensodyne Rapid Relief(®), a commercial desensitizing toothpaste, and also after artificial saliva (AS) immersion or citric acid challenge. Materials and methods: Dentine discs from human mandibular t...

Objectives: The aim of this study was to compare the results of dimethylthiazol diphenyltetrazolium bromide (MTT) and neutral red uptake (NRU) assays of Clinacanthus nutans cytotoxicity. Materials and methods: Mouse fibroblast (L929) cells were exposed to 0.01%, 0.1%, 0.25%, and 0.5% (W/V) C. nutans in a 96-cluster-well-culture plate for 24 h. T...

The aim of this study was to evaluate the cytotoxicity of three dentine bonding agents (G-Bond, Clearfil S(3) Bond and Clearfil SE Bond X) in cell-culture perfusion. In this experiment, 8×10(4) TCPC SV40 cells (bovine-pulp-derived cells transfected with simian virus 40 large T-antigen) in MEM-alpha media, 20%FCS were seeded on mesh in a 6-well plat...

This study evaluated the cytotoxicity of 3 self-etching bonding systems: Optibond Solo Plus SE primer, Xeno III, and i Bond. The test materials were applied on the dentine discs of dentine barrier models in the same way as in the clinical procedures recommended by each manufacturer. 3-D cell culture of Bovine pulp-derived cells transfected with Sim...

The objective of this study was to evaluate the cytotoxicity of furcal perforation repair materials, GI and MTA, using cell culture technique. The extract of ProRoot MTA and Ketac Molar were treated on PDL cells in a 96-well tissue-culture plate. Cell proliferation after an incubation period of 3 days was determined by using MTT assay. The growth o...

The purpose of this study was to compare the cytotoxicity of 2 gutta-percha solvents, chloroform and GP-Solvent, on cell line L929. 2 gutta-percha solvents were diluted into the concentrations of 1:100, 1:400, and 1:800. The experiment was done in a 96-well tissue-culture plate. Cell viability of L929 was determined after each gutta-percha solvent...

This study evaluated the cytotoxicity of four single component dentin bonding agents: Syntac Single Component, Prime & Bond 2.1, Single Bond and One Up Bond F. The test materials were applied on dentin discs of dentin barrier models in the same way as in the clinical procedures recommended by each manufacturer. Cell viability of L 929 after exposur...

Samples of a conditioned medium (CM), obtained from human ginvigal fibroblasts culture, were kept at 4 degrees C each for 2 weeks, 1, 2, 3 and 6 months. Their effectiveness in maintaining cell survival for their contact with L929 cells in 96 well tissue culture plates for 6 and 10 h at 4 degrees C was compared with Hank's Balanced Salt Solution (HB...

An evaluation of the accuracy of the "Root ZX" apex locator was conducted on 20 single-rooted teeth that had been extracted because of severe disease or orthodontic treatment. After the pulp chamber had been accessed and the tooth length determined using the Root ZX apparatus, the file was fixed to the tooth with light-cured composite resin. After...

The aim of this study was to evaluate Sulphorhodamine-B (SRB) staining against 51Cr-release in cytotoxicity tests of six endodontic sealers, namely, MU sealer (Mahidol University) ROCANAL 2, ROCANAL 3, Apexit, Endomethasone, and AH-26. Monolayers (5 x 10(5) cells/ml) of the mouse cell line Mu-mu-1 were used as test cells. Following incubation at 37...

The aim of this study was to evaluate the response of a multilayer compared with a monolayer cell culture using six root canal sealers. Both monolayer and multilayer of MU-mu-1 (Mahidol University mouse cell line 1) were cultured in separate 96-well plates. Following incubation at 37 degrees C in 5% CO2 for 4 h in the presence of each sealer, cells...

A case of "double" dens in dente with an open apex and periapical lesion is reported. Nonsurgical endodontic treatment was performed. The canals were medicated for 5 months with calcium hydroxide before obturation with combined sectional and thermoplasticized warm gutta-percha. Follow-up revealed an asymptomatic patient and a reduction in size of t...

A case report of endodontic surgery of maxillary left lateral incisor with external root resorption which perforated the root canal was presented. The cause of this abnormality was due to chronic inflammation. The patient had the history of pain and swelling. After root canal filling by thermoplasticized warm gutta-percha technique, the resorption...