L. S. O’Donoughue

L. S. O’Donoughue
Centre de Recherche sur les Grains Inc. (CÉROM) | CEROM · Oilseeds genetics

About

49
Publications
7,607
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1,803
Citations
Citations since 2016
19 Research Items
717 Citations
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
Additional affiliations
October 2010 - present
Centre de Recherche sur les Grains Inc. (CÉROM)
Position
  • Senior Researcher

Publications

Publications (49)
Article
Full-text available
Genetic linkage maps are used to localize markers on the genome based on the recombination frequency. Most often, these maps are based on the segregation observed within a single biparental population of limited size (n<300) where relatively few recombination events are sampled and in which some genomic regions are monomorphic because both parents...
Article
Full-text available
The SoyaGen project was a collaborative endeavor involving Canadian soybean researchers and breeders from academia and the private sector as well as international collaborators. Its aims were to develop genomics-derived solutions to real-world challenges faced by breeders. Based on the needs expressed by the stakeholders, the research efforts were...
Article
Maximizing yield is very important when developing new cultivars. However, yield must usually be improved jointly with other key traits, which can prove challenging when they are unfavorably correlated. Genomic predictions can facilitate the selection of promising lines among the progeny of crosses, but it may also help in selecting crosses that ar...
Article
Lodging resistance is an important objective for soybean [Glycine max (L.) Merr.] breeding, but selection for this trait has been difficult since the resistance is controlled by multiple genes and these genes interact with the environment. One hundred thirty of 139 lines constituting a soybean GWAS panel were phenotyped for stem pushing resistance,...
Article
Full-text available
Here we describe a worldwide haplotype map for soybean (GmHapMap) constructed using whole‐genome sequence data for 1,007 Glycine max accessions and yielding 14.9 million variants as well as 4.3M tag single nucleotide polymorphisms (SNPs). When sampling random subsets of these accessions, the number of variants and tag SNPs plateaued beyond approxim...
Article
Full-text available
The soybean cyst nematode (SCN) generates more damage to soybean than any other parasite in most soybean‐producing countries. The use of SCN‐resistant cultivars remains the most effective method to limit losses caused by SCN. The SCN‐resistant accession PI 88788 has been used almost exclusively to control SCN over the past decades, inducing a shift...
Article
Full-text available
Core ideas: A gene-centric approach for haplotype definition was developed and implemented in R. The tool allows for allelic characterization at given loci in germplasm collections. Allelic status at four maturity genes is predicted on the basis of marker genotyping data. Assessing the allelic diversity within a germplasm collection and identifyin...
Article
Full-text available
Soybean (Glycine max (L.) Merr.) is the most important legume in the world(Dorff, 2007; Shoemaker et al., 1996). However, the rapid expansion of its cultivated areas, has created new ecological niches for many pathogens. Among them, Phytophthora sojae (Kaufmann and Gerdemann) ‐ ranks as one of the most damaging soybean pests in the world. The most...
Article
Full-text available
A molecular linkage map of cultivated oat composed of 561 loci has been developed using 7 1 recombinant inbred lines from a cross between Avena byzantina cv. Kanota and A. sativa cv. Ogle. The loci are mainly restriction fragment length polymorphisms detected by oat cDNA clones from leaf, endosperm, and root tissue, as well as by barley leaf cDNA c...
Article
Full-text available
A molecular linkage map of cultivated oat composed of 561 loci has been developed using 7 1 recombinant inbred lines from a cross between Avena byzantina cv. Kanota and A. sativa cv. Ogle. The loci are mainly restriction fragment length polymorphisms detected by oat cDNA clones from leaf, endosperm, and root tissue, as well as by barley leaf cDNA c...
Preprint
Full-text available
Here we describe the first worldwide haplotype map for soybean (GmHapMap) constructed using whole-genome sequence data for 1,007 Glycine max accessions and yielding 15 million variants. The number of unique haplotypes plateaued within this collection (4.3 million tag SNPs) suggesting extensive coverage of diversity within the cultivated germplasm....
Article
Un inventaire des maladies racinaires du soya causées par des champignons et des oomycètes a été réalisé en 2014 et 2015 dans 80 champs situés en Montérégie et 4 au Centre-du-Québec. Douze plantules au stade de deux feuilles trifoliées ont été prélevées par champ pour évaluer l’état sanitaire de leurs racines (indice racinaire) selon une échelle de...
Article
Full-text available
Background: To continue to meet the increasing demands of soybean worldwide, it is crucial to identify key genes regulating flowering and maturity to expand the cultivated regions into short season areas. Although four soybean genes have been successfully utilized in early maturity breeding programs, new genes governing maturity are continuously b...
Poster
Full-text available
(Poster in french) Lors de fortes infestations, le puceron du soya, Aphis glycines Matsumura (Hemiptera: Aphididae), peut affecter le rendement des cultures de soya, tant en termes de quantité que de qualité, en raison de transmissions virales lors de sa prise alimentaire. Les semenciers désirant obtenir des lignées exemptes de virus, la résistance...
Poster
Full-text available
Electropenetrography (EPG) is a method largely used to study the feeding behaviour of piercing-sucking insects, notably aphids and cicadellids. It allows to record specific electrical signals (waveforms) when an insect inserts its mouthparts in the plant tissues. Depending on the type of signal, their frequency and their duration, it is possible to...
Article
Full-text available
Next-generation sequencing (NGS) and bioinformatics tools have greatly facilitated the characterization of nucleotide variation; nonetheless, an exhaustive description of both SNP haplotype diversity and of structural variation remains elusive in most species. In this study, we sequenced a representative set of 102 short-season soybeans and achieve...
Article
Full-text available
Soya bean is a major source of edible oil and protein for human consumption as well as animal feed. Understanding the genetic basis of different traits in soya bean will provide important insights for improving breeding strategies for this crop. A genome-wide association study (GWAS) was conducted to accelerate molecular breeding for the improvemen...
Article
Full-text available
In eastern Canada, earliness is an important trait for soybean given the short growing season. The aim of this work was to develop tools for breeders to rapidly identify alleles present in their germplasm at the recently cloned maturity locus E3 (GmPhyA3). The tremendous throughput of modern DNA sequencing technology has allowed the use of genotypi...
Article
There is an increased interest of bedding plant growers and consumers for environmentally friendly agricultural practices. Recently, products called organic-based fertilizers have appeared on the market. These products, in addition to the organic matter, contain a source of soluble nitrogen making them interesting to use in a fertilization program....
Article
Full-text available
Recombinant inbred lines from the oat (Avena sativa L.) population Kanota × Ogle were analyzed in order to detect quantitative trait loci for Barley Yellow Dwarf resistance. Plants were scored for BYD symptoms in three locations (Urbana-Illinois, Ithaca-New York, and a greenhouse), and in four years (1992, 1993, 1994, and 1995). All plants were ino...
Article
An interspecific hybridization program was undertaken involving the nonshattering Lotus species L. conimbricensis and L. ornithopodioides, and species belonging to the Lotus corniculatus group, L. alpinus, L. japonicus, and L. burttii. Out of 12 cross combinations, 8 were successful. Two of these, L. alpinus × L. conimbricensis and L. burttii × L....
Article
Full-text available
A restriction fragment length polymorphism (RFLP) map for the A genome of Avena has been developed using F3 families from the cross A. atlantica × A. hirtula. The main source of markers were an oat cDNA and a barley cDNA library. A total of 194 RFLP markers was used, 192 of which were mapped or assigned to linkage groups. Seven main linkage groups,...
Article
A morphological study of hybrids in an interspecific hybridization program involving the nonshattering Lotus species L. conimbricensis and L. ornithopodioides and species belonging to the Lotus corniculatus group, L. alpinus, L. japonicus, and L. burttii, was undertaken. Two hybrids (L. alpinus × L. conimbricensis and L. burttii × L. ornithopodioid...
Article
Full-text available
Molecular markers for the crown rust resistance genes Pc38, Pc39, and Pc48 in cultivated oat (Avena sativa L.) were identified using near-isogenic lines and bulked segregant analysis. Six markers for Pc48, the closest being 6 cM away, were found in a Pendek-39 Pendek-48 (Pendek3948) population, but none was found in a Pendek-48 Pendek-38 (Pendek483...
Article
Full-text available
Molecular markers for the crown rust resistance genes Pc38, Pc39, and Pc48 in cultivated oat (Avena sativa L.) were identified using near-isogenic lines and bulked segregant analysis. Six markers for Pc48, the closest being 6 cM away, were found in a 'Pendek-39' x 'Pendek-48' (Pendek3948) population, but none was found in a 'Pendek-48' X 'Pendek-38...
Article
tide sequence analysis of clones derived from susceptible cultivar CDC Teal and resistant lines indicated the presence of three genes served domains in the gene(s) coding for the catalytic coding for the catalytic subunit of AHAS. Using a collection of T. subunit of AHAS. Point mutations resulting in proline aestivum cv. Chinese Spring aneuploid an...
Article
Full-text available
In spring-type oat ( Avena sativa L.), quantitative trait loci (QTLs) detected in adapted populations may have the greatest potential for improving germplasm via marker-assisted selection. An F(6) recombinant inbred (RI) population was developed from a cross between two Canadian spring oat varieties: 'Terra', a hulless line, and 'Marion', an elite...
Article
Full-text available
Molecular mapping of cultivated oats was conducted to update the previous reference map constructed using a recombinant inbred (RI) population derived from Avena byzantina C. Koch cv. Kanota x Avena sativa L. cv. Ogle. In the current work, 607 new markers were scored, many on a larger set of RI lines (133 vs. 71) than previously reported. A robust,...
Article
Miniature inverted-repeat transposable elements or MITEs represent a large superfamily of transposons that are moderately to highly repetitive and frequently associated with plant genes. These attributes were exploited in the development of a powerful marker technology called Inter-MITE polymorphism, or IMP, which involves the amplification between...
Chapter
Full-text available
The genus Avena is organized into 14 taxa (8 diploid, 5 tetraploid, and 1 hexaploid) classified on the basis of chromosome number, genome, diaspore (unit of dispersal), flower morphology, and cross fertility (Ladizinsky 1989). Based on chromosome pairing and structure, diploid, tetraploid, and hexaploid species were given the genomic designations A...
Article
Full-text available
 Oat groats are unique among cereals for the high level and the embryo-plus-endosperm localization of lipids. Genetic manipulation of groat quality traits such as oil is desired for optimizing the value of oat in human and livestock diets. A locus having a major effect on oil content in oat groats was located on linkage group 11 by single-factor an...
Article
Full-text available
Genetic progress through selection is directly related to the amount of variability present in the population and the quality of genes contributed by the parents. Genetic relationships between lines were studied using DNA marker-based estimates of genetic similarity. A statistical methodology using the width of a confidence interval was developed t...
Article
Oat (Avena sativa L.) genotypes differ in their patterns of growth and development in response to vernalization (cold temperatures applied to germinating seeds). Genomic regions controlling vernalization response in heading date, plant height, and tiller number were mapped in a recombinant inbred (RI) population derived from the cross of oat cultiv...
Article
Molecular markers have been identified in cultivated oat for the Pg9 and Pg13 loci conferring resistance to different races of the stem rust pathogen, Puccinia graminis f. sp. avenae. Near-isogenic lines and bulked segregant analysis were used to identify putative restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA...
Article
Full-text available
The development of RFLP linkage maps in hexaploid and diploid oat allows us to study genetic relationships of these species at the DNA level. In this report, we present the extension of a previously developed diploid oat map (Avena atlantica x A. hirtula) and its molecular-genetic relationships with wheat, rice and maize. Examination of 92–99% of t...
Article
A molecular linkage map of cultivated oat composed of 561 loci has been developed using 71 recombinant inbred lines from a cross between Avena byzantina cv. Kanota and A. sativa cv. Ogle. The loci are mainly restriction fragment length polymorphisms detected by oat cDNA clones from leaf, endosperm, and root tissue, as well as by barley leaf cDNA cl...
Article
Full-text available
Daylength insensitive accessions of Avena sativa L. are being used to develop cultivars that will flower normally when grown under short or long photoperiods. Field data indicate that the insensitivity trait is under the control of a single dominant gene, designated Di1. The random amplified polymorphic DNA (RAPD) technique and bulk segregant analy...
Article
While anther culture or pollinations with Hordeum bulbosum have provided suitable methods for haploid production in bread wheat, they have been largely unsuccessful in durum wheat. Pollinations with maize were used in an attempt to produce haploid seedlings and, from these, fertile doubled haploids of durum wheats. Moreover, the effect of various c...
Article
Ten different tetraploid wheat (Triticum turgidum) genotypes were pollinated with maize (Zea mays). Fertilization was achieved in all ten genotypes and no significant difference in fertilization frequency between the tetraploid wheat genotypes was detected. A mean of 41.1% of pollinated ovaries contained an embryo. All these crosses were characteri...
Article
Interspecific Hybridization (wide-crossing) is a valuable technique both in fundamental plant genetics and in practical plant breeding programs. It has been particularly successful in cereal species, where its applications can be considered under two broad headings. Firstly, karyotypically stable crosses, which give rise to hybrid plants, can be us...
Chapter
The genus Avena is organized into 14 taxa, each representing a biological species (8 diploid, 5 tetraploid, and 1 hexaploid taxa) classified on the basis of chromosome number, genome, diaspore (unit of dispersal), flower morphology, and cross fertility (Ladizinsky 1989). Based on chromosome pairing and structure, diploid, tetraploid and hexaploid s...
Chapter
“The view generally entertained by naturalists is that species, when intercrossed, have been specially endowed with the quality of sterility, in order to prevent the confusion of all organic forms.” With these words Charles Darwin opens his chapter on hybridism in The Origin of Species. Darwin, however, goes on to argue that the degree of sterility...
Conference Paper
Full-text available
In Eastern Canada, earliness is an important trait given the short growing season. The aim of this work was to develop markers/haplotypes for breeders to rapidly assess the alleles present in their germplasm at the four recently cloned maturity genes (E1 to E4). The tremendous throughput of modern DNA sequencing technology, has allowed the use of g...

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