L. Hillejan

• Niels-Stensen-Kliniken, Krankenhaus St. Raphael, Ostercappeln

Simple Summary Lung cancer is the leading cause of cancer related deaths and non-small cell lung cancer (NSCLC) is its most relevant subtype. Here, we present data on p53 co-playing 5′-Nucleotidase Domain-Containing Protein 2 (NT5DC2) protein- and gene-expression and its clinical implication and prognostic value. NT5DC2 overexpression was associate...

Non-small cell lung cancer (NSCLC) is the leading cause of cancer death worldwide underlining the urgent need for new biomarkers and therapeutic targets for this disease. Long noncoding RNAs are critical players in NSCLC but the role of small RNA species is not well understood. In the present study, we investigated the role of H/ACA box small nucle...

Background: T cell infiltration in non-small cell lung cancer (NSCLC) is essential for the immunological response to malignant tissue, especially in the era of immune-checkpoint inhibition. To investigate the prognostic impact of CD4+ T helper cells (Th), CD8+ cytotoxic (Tc) and FOXP3+ regulatory T (Treg) cells in NSCLC, we performed this analysis...

Background: Epithelial-to-mesenchymal transition (EMT) is a crucial step in lung cancer pathogenesis. Among others, cancer-associated fibroblasts (CAFs) are reported to regulate this process. Objectives: To investigate the prognostic and clinical impact, we analyzed CD34+ and SMA+ CAFs in non-small cell lung cancer (NSCLC). Methods: Retrospect...

Objectives PSMA (prostate-specific membrane antigen) is overexpressed in prostate cancer cells and is reported to be a promising target for antibody-based radioligand therapy in patients with metastasized prostate cancer. Since PSMA expression is not restricted to prostate cancer, the underlying study investigates PSMA expression in non-small cell...

Correlation of clinicopathological variables with PSMA expression in NSCLC patients depending on tumor histology and grading. (DOCX)

[This corrects the article DOI: 10.1371/journal.pone.0177146.].

Background Aminopeptidase N (CD13) is a zinc-binding protease that has functional effects on both cancerogenesis and tumor angiogenesis. Since CD13 is an antigen suitable for molecular targeted therapies (e.g. tTF-NGR induced tumor vascular infarction), we evaluated its impact in NSCLC patients, and tested the effects of the CD13-targeted fusion pr...

Mouse trial: A549 Xenotransplants (tTF-NGR vs. PBS; raw data). (PDF)

Representative example for CD31 expression in NSCLC microvessels. (EPS)

The prognostic impact of CD13 in lung cancer with regard to univariate analyses. (PDF)

FACS analysis (A 549; raw data). (PDF)

NSCLC study collective (CD13 expression; raw data). (PDF)

Objectives: Several blood group-related carbohydrate antigens are prognosis-relevant markers of tumor tissues. A type 3 (repetitive A) is a blood group antigen specific for A1 erythrocytes. Its potential expression in tumor tissues has so far not been examined. Material and methods: We have evaluated its expression in normal lung and in lung can...

A lung abscess is an infectious pulmonary disease characterised by the presence of a pus-filled cavity within the lung parenchyma. The content of an abscess often drains into the airways spontaneously, leading to an air-fluid level visible on chest X-rays and CT scans. Primary lung abscesses occur in patients who are prone to aspiration or in other...

Epigenomic changes are an important feature of malignant tumors. How tumor aggressiveness is affected by DNA methylation of specific loci is largely unexplored. In genome wide DNA methylation analyses we identified the KCa 3.1 channel gene (KCNN4) promoter to be hypomethylated in an aggressive non-small cell lung carcinoma (NSCLC) cell line and in...

Pneumothorax is defined as an accumulation of air between the parietal and visceral pleura followed by lung collapse. Whereas primary pneumothorax occurs without an appa- rent cause and in the absence of significant lung disease, secondary pneumothorax is associated with underlying lung disease (for example COPD, lung fibrosis or malignent tumor)....

Background Sleeve lobectomy (SL) is considered a valid therapeutic option in untreated, centrally located non-small cell lung cancer (NSCLC) even in patients “fit” for pneumonectomy (PN). Nevertheless, SL feasibility and long-term results after induction therapy (IT) have been only rarely investigated. We herein report the results of a multicenter...

e18511 Background: Neoadjuvant platinum-based chemotherapy in stage III NSCLC offers the chance to eradicate occult metastases and decrease tumor volume, thus improving curative surgical resection rates. A remission after induction therapy is correlated with prolonged survival. However, only 40-50% of patients respond to therapy since there is stil...

Alterations of Eph receptor tyrosine kinases are frequent events in human cancers. Genetic variations of EPHB6 have been described but the functional outcome of these alterations is unknown. The current study was conducted to screen for the occurrence and to identify functional consequences of EPHB6 mutations in non-small cell lung cancer. Here, we...

The aim of this study was to evaluate the safety and efficacy of an original technique of single-port video-assisted thoracoscopy (S-VATS) for the minimally invasive treatment of pleural empyema in fibrinopurulent stage. Single-port video-assisted thoracoscopy was performed under general anesthesia and single-lung ventilation using a 2-cm incision...

Zielsetzung: Ziel der vorliegenden Studie war die Evaluierung der Durchfuhrbarkeit, des Risikoprofils sowie der Effektivitat der Single-Port videoassistierten Thorakoskopie (S-VATS) zur minimal-invasiven Behandlung des Pleuraempyems im Stadium I-II. Methode: Es handelt sich um eine retrospektive Analyse der Daten 61 konsekutiver, einer S-VATS unter...

Objective: To investigate the prognostic significance of location and extent of lymph node metastasis in resected non-small cell lung cancer (NSCLC), and to weigh up the influence of treatment modalities on survival. Patients and method: On exploratory analysis, patients were grouped according to location and time of diagnosis of nodal metastasis:...

The functions of large noncoding RNAs (ncRNAs) have remained elusive in many cases. Metastasis-Associated-in-Lung-Adenocarcinoma-Transcript-1 (MALAT-1) is an ncRNA that is highly expressed in several tumor types. Overexpression and RNA interference (RNAi) approaches were used for the analysis of the biological functions of MALAT-1 RNA. Tumor growth...

The aim of the present study was to evaluate the feasibility and diagnostic value of repeat mediastinoscopy as part of the response-evaluation protocol of 2 phase II multimodality studies for either stage IIIA/B non-small cell lung cancer or small cell lung cancer. From January 1991 through December 1998, 104 patients (79 men and 25 women) with sta...

Using semi-quantitative microarray technology, almost every one of the approximately 30 000 human genes can be analyzed simultaneously with a low rate of false-positives, a high specificity, and a high quantification accuracy. This is supported by data from comparative studies of microarrays and reverse-transcription PCR for established cancer gene...

Repeat cervical mediastinoscopy is a diagnostic surgical procedure for preoperative nodal staging in patients with insufficient first mediastinoscopy, with recurrent or second primary lung neoplasms, and following induction chemotherapy or chemo-/radiotherapy for locally advanced lung cancer. The aim of this study was to critically analyse indicati...

Patients with N1 non-small cell lung cancer represent a heterogeneous population with varying long-term survivals. Prognosis and pattern of recurrence seem to be particularly affected by the level of lymph node involvement. From 1990 to 1995, a total of 1954 consecutive patients underwent surgical resection for non-small cell lung cancer: 549 (28%)...

The locally advanced (T3 - 4) non-small cell lung cancer with pulmonary lymph node metastases (N1) is a mixture of different subgroups of disease with varying pattern of tumor extension and long-term survival rates. We retrospectively reviewed hospital records and follow-up data of 181 patients operated on between 1990 and 1995 with pathological st...

Hintergrund: Das lokal fortgeschrittene (T3 - 4) nicht-kleinzellige Bronchialkarzinom mit pulmonalen Lymphknotenmetastasen (N1) beinhaltet unterschiedliche Tumormanifestationen, die verschiedenen lymphatischen Metastasierungsmustern und möglicherweise Prognosen entsprechen. Patienten und Methode: Retrospektiv wurden die Krankenblätter und Verlaufsu...

Pulmonary tuberculosis has become a rare indication for surgical intervention in all industrial nations. Over a period of 10 years we overview 193 patients who were suffering this disease and underwent thoracotomy. Main indication (79.8 %) was pulmonary nodules, of unknown origin. In this cases wedge resection was performed. Expanded resectional te...

Operationen wegen einer Lungentuberkulose sind auch in thoraxchirurgischen Zentren zunehmend seltener. Im Verlauf eines 10-Jahres-Zeitraums wurden in unserer Klinik 193 Patienten operiert. Hauptindikation (79,8 %) ist heute der anders nicht auszuraumende Karzinomverdacht bei Rundherden und lokal begrenzten Infiltraten. Uberwiegend erfolgen hierbei...

Overall prognosis of patients with locally advanced non-small-cell lung cancer (LAD-NSCLC) is still unfavourable. Different attempts to improve treatment results have been made using combinations of chemotherapy and radiotherapy. The aim of this pilot phase I/II investigation was to test the feasibility and toxicity of a definitive multimodality pr...

Lung volume reduction surgery (LVRS) is effective in the short and intermediate term for the improvement of pulmonary function and subjective symptoms in selected patients with advanced emphysema. The purpose of this study was to examine the long-term functional results of LVRS and to investigate which subgroups would benefit in terms of long-term...

There are few current reports of surgical management for bronchiectasis because of its decline in prevalence, and it remains controversial as to which subgroups of patients would benefit from surgical management. We reviewed the medical records of all patients who underwent surgical resection for bronchiectasis between January 1, 1990, and December...

Completion pneumonectomy is reported to be associated with high morbidity and mortality, especially when done in patients with benign disease. We review our 9 years of experience with this operation to evaluate the postoperative outcome and long-term results of various indications. Between January 1990 and December 1998, 66 consecutive patients und...

Patients with unfavorable stages of lung cancer are rarely cured with local treatment modalities alone. Aim of our phase II trial was to investigate the effectivity of a multimodality treatment. Ninety-four patients with NSCLC (stage IIIA/IIIB) were treated preoperatively with chemoradiotherapy (cisplatin and etoposide, 45 Gy hyperfractionated acce...

All patients being considered for pulmonary resection require a careful evaluation. The radiographic imaging procedure basically includes the native x-ray of the chest and computer tomography scanning (CT). Magnetic resonance imaging (MRI) as well as angiography and positron emission tomography (PET) apear to improve the accuracy of staging, but sh...

Patients with unfavorable stages of lung cancer are rarely cured with local treatment modalities alone. Aim of our phase II trial was to investigate the effectivity of a multimodality treatment. Ninety-four patients with NSCLC (stage IIIA/IIIB) were treated preoperatively with chemoradiotherapy (cisplatin and etoposide,45 Gy hyperfractionated accel...

Preoperative chemoradiotherapy is feasible for selected patients with non-small cell lung cancer stage IIIb. The aim of this investigation was to analyze long-term results after this multimodality approach and to identify subgroups with improved long-term prognosis. From March 1991 to June 1996, 56 patients were entered. Three cycles of cisplatin (...

From 1991 to 1993 110 patients with dislocated fractures of the distal radius were treated at the Marienhospital Herne (Ruhr-University Bochum). 86 patients were reexamined for a follow-up of 22 (5-51) months at average. Regarding the anatomical results and wrist function nearly all patients with type A2 and C1 fracture according AO-classification...

Despite modern diagnostic methods and appropriate treatment, pleural empyema remains a serious problem. Our purpose was to assess the feasibility and efficacy of the video-assisted thoracoscopic surgery in the management of nontuberculous fibrinopurulent pleural empyema after chest tube drainage treatment had failed to achieve the proper results. W...

From January 1990 to December 1995 79 patients with mediastinal lesions were seen for parasternal mediastinoscopy at the Ruhrlandklinik Essen. Diagnosis was achieved in 91.1%. In 7 cases (8.9%) the diagnosis was not established. Six intraoperative complications occurred following mediastinoscopy: minor bleeding in five instances from the internal m...

Torsion of the gallbladder with onset of gangrene is a rare condition which is generally due to an abnormal anatomical variation: loss of the fixation of the organ to the inferior margin of the liver with the presence of a long mesocyst. This case shows that ultrasound is able to make the right diagnosis which was so often confirmed not before lapa...

Etiology of primary varicose veins remains uncertain. Pathophysiological mechanisms of varicosis bases on lack of smooth muscle tone as well as incompetence of the sapheno-femoral valve and the perforating veins. In order to conserve as many healthy veins as possible for future vein grafting only varicose veins should be selected for stripping. Pre...

Hip arthroplasty using a bipolar prosthesis was performed on 143 patients between 1988 and 1993. The average patient age at surgery was 81 years; 13 percent died during their hospital stay. By sending questionnaires to the patients and making inquiries of the General Practitioner we received information about related complications and patients' reg...

Baker's cysts have to be regarded as a secondary phenomenon of knee-joint diseases. In the treatment of Baker's cysts, therefore, arthroscopy should be done before extirpation. In our study we wanted to look at the results of this therapeutic concept. In the surgical clinic within the Ruhr University of Bochum 34 patients with Baker's cysts underwe...

The combination of sarcoidosis of the thyroid gland with hyperthyroidism is extremely rare. We report on two patients who were operated on by an autoimmuno-thyroiditis in association with Graves' disease. Histology discovered sarcoidosis of the thyroid gland. Diagnostic measures, therapeutic procedures and follow-up are discussed with a review of t...