L. N. Borshchevskaya

State Research Institute of Genetics and Selection of Industrial Microorganisms | Genetika · Research Institute for Genetics and Selection of Industrial Microorganisms

The isolation, heterologous gene expression, and characteristics of a new β-glucanase from Bacillus pumilus is described. The bgl1 gene from the Bacillus pumilus Bg57 VKPM В-13195 strain, which consists of 729 nucleotides, encodes a secreted endo-β-l,3(4)-D-glucanase (EC 3.2.1.6) containing 214 amino acids and 28 residues of the putative signal pep...

Endo-1,4-β-xylanases from Bacillus pumilus VKPM В-7975, Paenibacillus polymyxa VKPM В-3015, Thermomyces lanuginosus VKPM F-224, and Schizophyllum commune VKPM F-433, which are members of a family of 11 glycohydrolases, have been obtained in the expression system of Pichia pastoris. Technologically valuable characteristics of the recombinant hydrola...

Phytases are widely used to improve the properties of mixed fodder. Due to its high specific activity, PhyА-Cf phytase from Citrobacter freundii is of special interest for agrobiotechnology. In order to decrease the thermal sensitivity of the enzyme during fodder granulation at 60–80°C, the thermal stability of phytase was enhanced by site-directed...

In¹ order to increase the expression level of gene for recombinant phytase in Pichia pastoris, a synthetic sequence of the phyOp-mod gene, which encodes phytase from Obesumbacterium proteus, has been designed and optimized by its codon composition. The GC content was adjusted to 48.33%, and AT-rich segments were eliminated. The main characteristics...

The expression of four genes of bacterial endo-β-l,3(4)-D-glucanases from B. pumilus Bg57 (VKPM В-13195), Paenibacillus polymyxa Bg23 (VKPM В-4056), Bacillus subtilis Bg11 (VKPM B-13178), and Bacillus amyloliquefaciens Bg76 (VKPM B-13184), which are members of the family of GH16 glycohydrolases, in the expression system of Pichia pastoris are compa...

A DNA sequence from Kosakonia sacchari that according to automated computer analysis is believed to correspond to a gene for histidine acid phytase has been selected from the GenBank database. The sequence was optimized for codon composition, synthesized, cloned and expressed in Pichia pastoris. Main characteristics of the purified recombinant enzy...

A Pichia pastoris yeast strain producing endo-l,4-β-xylanase from Paenibacillus brasilensis with an activity of 54,400 U/mL after 140 h of fermentation in a laboratory fermenter has been obtained. A number of approaches were used to increase the level of the xylanase production in this strain: optimization of the target gene codon composition, mult...

Effect of overexpression of the HAC1 genes from Pichia pastoris and Saccharomyces cerevisiae on the production of heterologous enzymes, Escherichia coli phytase and Paenibacillus brasilensis xylanase, in P. pastoris cells has been studied. Codon composition of the phytase and xylanase encoding genes was optimized, and the genes were expressed in P....

A method is proposed for the rapid identification of the taxa of five industrially valuable yeast groups: Pichia pastoris, Yarrowia lipolytica, Schizosaccharomyces pombe, Saccharomyces cerevisiae, and Kluyveromyces lactis. The specific primers selected based on nucleotide sequences of genes encoding rRNA were used in PCR. The method was effective i...

A stepwise screening of bacterial xylanase producers isolated from forest soil in Moscow oblast has been performed, and the taxa of the microorganisms have been identified based on an analysis of the 16S RNA gene sequences. An approach to the selection of the most active producers is suggested, and this selection has been carried out, resulting in...

Comparative analysis of the efficiency of the expression of the Escherichia coli, Obessumbacterium proteus, and Citrobacter freundii phytase genes in Pichia pastoris methylotrophic yeast has been carried out. A simple model with a genetic construct based on the pPICά vector and the plate test with a modified diagnostic medium containing methanol (i...

The expression of the genes for endo-1,4-β-xylanase from Bacillus pumilus VKPM â-7975, Paenibacillus polymyxa VKPM â-3015, Thermomyces lanuginosus VKPM F-224, and Schizophyllum commune VKPM F-433, members of the family of 11 glycohydrolases, in the expression system of Pichia pastoris has been assessed. Such technologically valuable characteristics...

In order to increase the level of expression of recombinant phytases in the Pichia pastoris yeast, a synthetic sequence of the phyOp-mod gene that encodes phytase from Obesumbacterium proteus has been developed and optimized by codon composition. The GC content was adjusted to 48.33, and the AT-rich segments were eliminated. The main characteristic...

The isolation, heterological gene expression and characteristics of a new β-glucanase from Bacillus pumilus has been described for the first time. A gene bgl1 from the Bacillus pumilus Bg57 VKPM В-13195 consisting of 729 nucleotides encodes a secreted endo- β-l,3(4)-d-glucanase ( EC 3.2.1.6) that contains 214 amino acids and 28 residues of the puta...

В экспрессионной системе Pichia pastoris проведено сравнение четырех бактериальных эндо-β- l,3(4)-D-глюканаз из Bacillus pumilus Bg57 ВКПМ В-13195, Paenibacillus polymyxa Bg23 ВКПМ В-4056, Bacillus subtilis Bg11 ВКПМ B-13178 и B. amyloliquefaciens Bg76 ВКПМ B-13184, относящихся к семейству GH16 гликозилгидролаз. Были исследованы такие технологическ...

Фитазы широко используются для улучшения питательных свойств комбикормов. Благодаря высокой удельной активности фитаза PhyА-Cf из Citrobacter freundii представляет особый интерес для агробиотехнологии. Для снижения инактивации фермента во время гранулирования корма при 60-80 °С была увеличена термостабильность фитазы PhyА-Cf методом сайт-направленн...

A stepwise screening of bacterial producers of xylanases isolated from the forest soil in Moscow oblast, and also the taxonomic identification of the microorganisms based on the analysis of the 16S RNA gene sequences have been performed. An approach to the selection of the most active producers was suggested, and this selection was carried out resu...

A method for a rapid taxonomy identification of five industrially valuable yeast groups, Pichia pastoris, Yarrowia lipolytica, Schizosaccharomyces pombe, Saccharomyces cerevisiae and Kluyveromyces lactis, has been suggested. The specific primers that were selected on the basis of nucleotide sequences of genes encoding rRNA were used in PCR. The met...

A comparative analysis of the efficiency of the Escherichia coli, Obessumbacterium proteus and Citrobacter freundii phytase gene expression in a Pichia pastoris methylotrophic yeast has been carried out. The level of the expression was assessed using a suggested for the first time simple model using a genetic construct based on the pPICά vector and...

The expression of L-lactate dehydrogenase genes ldh1 (Bos taurus), ldhA (Homo sapiens), ldhA (Rhizopus oryzae), ldh1 (Lactobacillus plantarum), and ldh1 (Lactobacillus pentosus) in the cells of yeast Schizosaccharomyces pombe VKPM U-3106 has been investigated. The catalytic characteristics of the enzymes encoded by these genes have been compared, a...

An efficient and inexpensive spectrophotometric method has been developed for the determination of lactic acid in the individual state and in food and biological and cultural liquids. The method is based on the spectrophotometric determination of the colored product of the reaction of lactate ions with iron(III) chloride at 390 nm. The optimum cond...

Two Bacillus subtilis strains isolated from the fruiting body of a basidiomycete fungus Pholiota squarrosa exhibited a broad range of antibacterial activity, including those against methicillin-resistant Staphylococcus aureus INA 00761 (MRSA) and Leuconostoc mesenteroides VKPM B-4177 resistant to glycopeptide antibiotics, as well as antifungal acti...

A method for the taxonomic identification of seven closely related bacterial species of the Bacillus subtilis group (B. subtilis, B. amyloliquefaciens, B. licheniformis, B. vallismortis, B. atrophaeus, B. sonorensis, and B. mojavensis) using specific primers selected on the basis of the gyrA gene sequences was developed. The effectiveness of this m...