Kyria Boundy-Mills

Kyria Boundy-Mills
University of California, Davis | UCD · Department of Food Science and Technology

PhD, Biochemistry

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83
Publications
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Publications

Publications (83)
Article
Proline is a predominant amino acid in grape must, but it is poorly utilized by the yeast Saccharomyces cerevisiae in wine-making processes. This sometimes leads to a nitrogen deficiency during fermentation and proline accumulation in wine. In this study, we clarified that a glucose response is involved in an inhibitory mechanism of proline utiliza...
Article
Full-text available
Yeasts are ubiquitous in temperate forests. While this broad habitat is well‐defined, the yeasts inhabiting it and their life cycles, niches, and contributions to ecosystem functioning are less understood. Yeasts are present on nearly all sampled substrates in temperate forests worldwide. They associate with soils, macroorganisms, and other habitat...
Article
Full-text available
The unambiguous application of fungal names is important to communicate scientific findings. Names are critical for (clinical) diagnostics, legal compliance, and regulatory controls, such as biosafety, food security, quarantine regulations, and industrial applications. Consequently, the stability of the taxonomic system and the traceability of nome...
Article
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The olive fruit fly (Bactrocera oleae Rossi) is the primary insect pest in all olive-growing regions worldwide. New integrated pest management (IPM) techniques are needed for B. oleae to mitigate reliance on pesticides used for its control which can result in negative environmental impacts. More effective lures for monitoring olive flies would help...
Conference Paper
Full-text available
The Phaff Yeast Culture Collection at University of California, Davis, allows researchers a unique opportunity to use yeasts that may be unavailable in any other case. The collection is one of the largest public compendiums of wild yeasts in the world, with over 7,500 strains belonging to 1,000 different species. The collection contains upwards of...
Article
The valorization of lignin is critical for the economic viability of the bioeconomy. Microbial metabolism is advantageous for handling the myriad of aromatic compounds resulting from lignin chemical or enzymatic depolymerization. Coupling aromatic metabolism to fatty acid biosynthesis makes possible the production of biofuels, oleochemicals, and ot...
Article
The olive fruit fly, Bactrocera oleae (Rossi), is one of the most damaging insect pests of olives worldwide, requiring the use of insecticides for fruit protection in many orchards. Olive fruit flies are attracted to volatile composunds, including a female-produced pheromone, and host-plant and bacterial volatiles. Preliminary laboratory bioassays...
Article
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Collections of microorganisms are a crucial element of life science research infrastructure but are vulnerable to loss and damage caused by natural or man‐made disasters, the untimely death or retirement of personnel, or the loss of research funding. Preservation of biological collections has risen in priority due to a new appreciation for discover...
Article
Lignocellulose pretreatment with ionic liquids (IL) enables release of fermentable sugars at yields suitable for biofuel production. However, despite extensive washing, residual IL such as 1-ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) is harmful to microbes and enzymes involved in downstream processes. Ionic liquid-pretreated switchgrass hydr...
Article
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Many adult Chrysoperla comanche (Stephens) green lacewings were caught in traps baited with live yeast cultures during tests designed to catch olive fruit flies. All 13 yeast species tested were more attractive than the industry-standard dried torula yeast (Cyberlindnera jadinii; syn. Candida utilis). Live C. jadinii culture attracted significantly...
Article
BACKGROUND Cassava leaves are an abundant global agricultural residue because the roots are a major source of dietary carbohydrates. Although cassava leaves are high in protein, the protein is not bioavailable. This work aimed to convert cassava leaves to a bioavailable protein‐rich animal feed ingredient using high‐protein yeasts. RESULTS The str...
Article
Full-text available
Five methylotrophic strains (UCDFST 71-1024T, UCDFST 54-11.16, UCDFST 54-11.141, UCDFST 68-967.1 and UCDFST 74-1030) from the Phaff Yeast Culture Collection (University of California Davis, USA) that were originally designated as Pichia pastoris were found to represent a novel Komagataella species. Strains of Komagataella mondaviorum sp. nov. UCDFS...
Article
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Genomic information is essential for taxonomic, phylogenetic and functional studies to comprehensively decipher the characteristics of microorganisms, to explore microbiomes through metagenomics, and to answer fundamental questions of nature and human life. However, large gaps remain in the available genomic sequencing information published for bac...
Article
Extracellular fungal glycolipid biosurfactants have attracted attention because productivities can be high, cheap substrates can be used, the molecules are secreted into the medium and the downstream processing is relatively simple. Three classes of extracellular fungal glycolipid biosurfactants have provided most of the scientific advances in this...
Article
Full-text available
Pretreatment with ionic liquids (IL) such as 1-ethyl-3-methylimidazolium chloride or acetate is an effective method for aiding deconstruction of lignocellulosic biomass; however, the residual IL remaining in hydrolysates can be inhibitory to growth of ethanologenic or oleaginous yeasts that have been examined in the literature. The aim of this stud...
Patent
Provided are methods and yeast cultures for producing polyol lipids and polyol lipid compositions.
Patent
Provided are methods and yeast cultures for producing polyol lipids and polyol lipid compositions. (https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2017184884&recNum=1&maxRec=&office=&prevFilter=&sortOption=&queryString=&tab=PCT+Biblio)
Article
Full-text available
The U.S. Culture Collection Network held a meeting to share information about how culture collections are responding to the requirements of the recently enacted Nagoya Protocol on Access to Genetic Resources and the Fair and Equitable Sharing of Benefits Arising from their Utilization to the Convention on Biological Diversity (CBD). The meeting inc...
Chapter
There are many well-known public yeast repositories as well as a large number of smaller, less-known collections worldwide; most of these are with the primary goal to preserve the yeast biodiversity in a specific region and the strains from a range of species that are important environmental strains, food spoilage organisms, or strains that play a...
Article
Microbial oils have been analyzed as alternatives to petroleum. However, just a handful of microbes have been successfully adapted to produce chemicals that can compete with their petroleum counterparts. One of the reasons behind the low success rate is the overall economic inefficiency of valorizing a single product. This study presents a lab-scal...
Article
Polyol esters of fatty acids (PEFA) are amphiphilic glycolipids produced by yeast that could play a role as natural, environmentally friendly biosurfactants. We recently reported discovery of a new PEFA-secreting yeast species, Rhodotorula babjevae, a basidiomycetous yeast to display this behavior, in addition to a few other Rhodotorula yeasts repo...
Article
Full-text available
In this study, we examined Sicilian-style green olive fermentations upon the addition of Saccharomyces cerevisiae UCDFST 09-448 and/or Pichia kudriazevii UCDFST09-427 or the lactic acid bacteria (LAB) Lactobacillus plantarum AJ11R and Leuconostoc pseudomesenteroides BGM3R. Olives containing S. cerevisiae UCDFST 09-448, a strain able to hydrolyze pe...
Article
Many scientists may not be aware of International Treaties impacting on their work. This article addresses the impact of the Nagoya Protocol on Access and Benefit Sharing on microbiology
Article
A multiplatform mass spectrometry-based approach was used for elucidating extracellular lipids with biosurfactant properties produced by the oleaginous yeast Rhodotorula babjevae UCDFST 04-877. This strain secreted 8.6 ± 0.1 g/L extracellular lipids when grown in a benchtop bioreactor fed with 100 g/L glucose in medium without addition of hydrophob...
Article
The twenty-first century has brought new opportunities and challenges to yeast culture collections, whether they are long-standing or recently established. Basic functions such as archiving, characterizing and distributing yeasts continue, but with expanded responsibilities and emerging opportunities. In addition to a number of well-known, large pu...
Article
Of 1600 known species of yeasts, about 70 are known to be oleaginous, defined as being able to accumulate over 20 % intracellular lipids. These yeasts have value for fundamental and applied research. A survey of yeasts from the Phaff Yeast Culture Collection, University of California Davis was performed to identify additional oleaginous species wit...
Article
The United States Culture Collection Network was formed in 2012 by a group of culture collection scientists and stakeholders in order to continue the progress established previously through efforts of an ad hoc group. The network is supported by a Research Coordination Network grant from the US National Science Foundation (NSF) and has the goals of...
Article
High-throughput, ‘next-generation’ sequencing tools offer many exciting new possibilities for food research. From investigating microbial dynamics within food fermentations to the ecosystem of the food-processing built environment, amplicon sequencing, metagenomics, and transcriptomics present novel applications for exploring microbial communities...
Article
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The sparse descriptions of the stick insect (Phasmatodea) digestive system as reported/provided in the literature are highly contradictory. This paper describes the digestive systems of several families of Phasmatodea (Timematidae, Heteropterygidae, Diapheromeridae, Pseudophasmatidae, and Phasmatidae) plus the gut microbiome of these and one other...
Article
Full-text available
The mission of the United States Culture Collection Network (USCCN, http://usccn.org) is "to facilitate the safe and responsible utilization of microbial resources for research, education, industry, medicine, and agriculture for the betterment of human kind." Microbial culture collections are a key component of life science research, biotechnology,...
Article
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This report summarizes the activities and outcomes of the second workshop of the US Culture Collection Network, formally an activity of the US National Science Foundation sponsored Research Coordination Network for a Community of ex situ Microbial Germplasm Repositories. The workshop included presentations on topics as diverse as permitting for gen...
Article
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Production of biodiesel from edible plant oils is quickly expanding worldwide to fill a need for renewable, environmentally-friendly liquid transportation fuels. Due to concerns over use of edible commodities for fuels, production of biodiesel from non-edible oils including microbial oils is being developed. Microalgae biodiesel is approaching comm...
Article
Lignocellulosic plant biomass is the target feedstock for production of second-generation biofuels. Ionic liquid (IL) pretreatment can enhance deconstruction of lignocellulosic biomass into sugars that can be fermented to ethanol. Although biomass is typically washed following IL pretreatment, small quantities of residual IL can inhibit fermentativ...
Article
Full-text available
Microbial oil is a potential alternative to food/plant-derived biodiesel fuel. Our previous screening studies identified a wide range of oleaginous yeast species, using a defined laboratory medium known to stimulate lipid accumulation. In this study, the ability of these yeasts to grow and accumulate lipids was further investigated in synthetic hyd...
Article
Full-text available
Conversion of lignocellulosic hydrolysates to lipids using oleaginous (high lipid) yeasts requires alignment of the hydrolysate composition with the characteristics of the yeast strain, including ability to utilize certain nutrients, ability to grow independently of costly nutrients such as vitamins, and ability to tolerate inhibitors. Some combina...
Article
ABSTRACT In recent years attention has been focused on the utilization of microorganisms as alternatives for industrial and nutritional applications. Considerable research has developed techniques for growth, extraction and purification of high value lipids such as biofuels, biosurfactants, as well as high value metabolites for nutrition and health...
Chapter
Collections of living microbes exist in a number of scales and with different degrees of permanence and formality. Many organizations have an interest in the activities and success of these collections. Modern genome data are both dependent on culture collections and transforming the fundamental nature of the collections at the same time. The abili...
Chapter
Full-text available
Cold-adapted (psychrophilic and psychrotolerant) yeasts have been isolated from a variety of substrates, using a variety of cultivation methods. Yeasts able to grow at as low as 0 °C have been isolated from cold substrates such as glaciers, snow, and deep-sea sediment, but also from temperate and tropical climates. A broad diversity of media and cu...
Article
Full-text available
A rapid and inexpensive method for estimating lipid content of yeasts is needed for screening large numbers of yeasts samples. Nile red is a fluorescent lipophilic dye used for detection and quantification of intracellular lipid droplets in various biological system including algae, yeasts and filamentous fungi. However, a published assay for yeast...
Conference Paper
Background / Purpose: The purpose of these studies was to determine whether eusocial wasps were orienting to volatiles produced by a ubiquitous microbial species. We also wanted to determine whether wasps vectored the microbes they were attracted to. Main conclusion: Wasps were attracted to fungal isolates in the field, and to synthetic chemic...
Article
Full-text available
Drosophila melanogaster adults and larvae, but especially larvae, had profound effects on the densities and community structure of yeasts that developed in banana fruits. Pieces of fruit exposed to adult female flies previously fed fly-conditioned bananas developed higher yeast densities than pieces of the same fruits that were not exposed to flies...
Data
Evidence that Candida californica , C. zemplinina and Pichia kluyveri are “substrate-generalists”. (DOCX)
Data
Larval fecal pool size and size at emergence as a function of fruit diet. (DOCX)
Data
Larval processing decreased the variability of yeast communities on banana fruits. Multivariate dispersion was measured as the mean distance to the centroid of a treatment group in principal component space. Both the larvae and the larvae and adult treatments were significantly different from the control group (p<0.001). (TIF)
Data
Additional information on isolines. (DOCX)
Data
Ordination of yeast community composition on the first two principal coordinates (PCoA1 and PCoA2). Each open symbol or cross represents a yeast community. The colored polygons represent the ordination hull encompassing each of the four treatment groups: green = adults, blue = no flies, violet = larvae, and orange = adults and larvae. The centroids...
Data
Yeast species richness for four treatment groups. Flies tended to reduce variability in the species richness of yeast communities. With adult and larvae present, the majority of yeast communities contained three yeast species. (TIF)
Article
Full-text available
Microbes are ubiquitous on plant surfaces. However, interactions between epiphytic microbes and arthropods are rarely considered as a factor that affects arthropod behaviors. Here, volatile emissions from an epiphytic fungus were investigated as semiochemical attractants for two eusocial wasps. The fungus Aureobasidium pullulans was isolated from a...
Article
Full-text available
A rich history of investigation documents various Drosophila-yeast mutualisms, suggesting that Drosophila suzukii similarly has an association with a specific yeast species or community. To discover candidate yeast species, yeasts were isolated from larval frass, adult midguts, and fruit hosts of D. suzukii. Terminal restriction fragment length pol...
Article
Full-text available
The importance of selecting optimal yeast strains for research or industrial applications is often underestimated. For example, utilizing a strain background that already provides the desired stress tolerance or nutrient utilization profile can eliminate costly strain optimization. Yeast culture collections can provide not only the yeast strains bu...
Chapter
This chapter studies the genus Saccharomycodes. In the determination of the asexual reproduction it is seen that cells are lemon-shaped (apiculate) and divide by bipolar budding on a broad base (bud-fission). Pseudohyphae are absent or poorly developed. In sexual reproduction it is found that the ascospores are spherical; a narrow subequatorial led...
Chapter
Full-text available
This chapter studies the genus Cyniclomyces. In the determination of asexual reproduction, cells are long-ovoid to cylindrical and divide by budding at the poles or at the shoulders of the parent cell. In liquid media, cells occur in branched chains, but on solid media the cells occur mainly singly or in pairs. In sexual reproduction it is seen tha...
Article
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A high-throughput (HT) paradigm generating LC-MS-UV-ELSD-based natural product libraries to discover compounds with new bioactivities and or molecular structures is presented. To validate this methodology, an extract of the Indo-Pacific marine sponge Cacospongia mycofijiensis was evaluated using assays involving cytoskeletal profiling, tumor cell l...
Conference Paper
Full-text available
The spotted wing drosophila, Drosophila suzukii Matsumura (Diptera: Drosophilidae), is a native of Southeast Asia and a pest of berry and stone fruit crops. In 2008, following its identification in North America, caneberries had total revenue losses of $89.8 million dollars, more than any other Californian host. A closely related species Drosophila...
Conference Paper
Previous research has shown that microbes are associated with bee pollinators in brood provisions, nest material, larval bee cadavers, and on the tongues of foraging adult bees. Less understood, however, is if the microbial communities associated with solitary bees change over time, or if these communities are consistently present throughout the ac...
Article
Rapid Identification and Classification of Wine Microbes by MALDI-TOF Mass Spectrometry Michael S. Ramsey†, Nannan Tao, Gongyi Shi, Santiago Ruiz-Moyano, C.M. Lucy Joseph, Kyria Boundy-Mills and David A. Mills* + Author Affiliations Department of Viticulture and Enology, University of California, Davis, CA 95616 (damills@ucdavis.edu) Matrix-as...
Article
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Terminal restriction fragment length polymorphism (TRFLP), used most often to describe bacterial communities, presents a high-throughput, low-cost solution for analyzing mixed yeast communities in wine and other fermentations. In this study, a TRFLP approach was developed for the identification and discrimination of yeasts and used to construct a T...
Article
Full-text available
A novel species of ascomycetous yeast, Pichia insulana sp. nov., is described from necrotic tissue of columnar cacti on Caribbean islands. P. insulana is closely related to and phenotypically very similar to Pichia cactophila and Pichia pseudocactophila. There are few distinctions between these taxa besides spore type, host preference and locality....
Article
The teleomorphic basidiomycetous yeast Xanthophyllomyces dendrorhous is important as a commercial source of astaxanthin, which is a component of feeds for mariculture. Phaffia rhodozyma is the anamorphic state of Xanthophyllomyces; however, there are conflicting reports in the literature concerning the presence of a sexual cycle in P. rhodozyma. Th...
Article
Full-text available
Refractory periods at the onset of dispersal occur when individuals initially do not settle in response to cues from naturally occurring habitats. The current paper focuses on relationships between refractory periods and habitat selection behaviour when natal dispersers use sequential search tactics. We first show how individual differences in acce...
Article
Ten different versions of the D1/D2 divergent domain of the large-subunit ribosomal DNA were identified among interbreeding members of the yeast species Clavispora lusitaniae. One major polymorphism, located in a 90-bp structural motif of the D2 domain, exists in two versions that differ by 32 base substitutions. Three other polymorphisms consist o...
Chapter
A 21.5-kilobase EcoRI genomic DNA fragment from Pseudomonas sp. strain ADP, designated pMD1, was shown to encode atrazine degradation activity in Escherichia coli DH5α. Atrazine degradation was demonstrated by a zone-clearing assay on agar medium containing crystalline atrazine and by chromatographic methods. A gene conferring the atrazine clearing...
Article
Full-text available
We previously reported the isolation of a 21.5-kb genomic DNA fragment from Pseudomonas sp. strain ADP, which contains the atzA gene, encoding the first metabolic step for the degradation of the herbicide atrazine (M. de Souza, L. P. Wackett, K. L. Boundy-Mills, R. T. Mandelbaum, and M. J. Sadowsky, Appl. Environ. Microbiol. 61:3373-3378, 1995). In...