Kylie Bucalo

Kylie Bucalo
Atlanta Botanical Garden · Conservation Research

Masters of Science

About

15
Publications
2,582
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338
Citations
Additional affiliations
January 2004 - January 2012
Georgia Institute of Technology
Position
  • Laboratory Manager

Publications

Publications (15)
Article
Full-text available
Orchidaceae is among the largest plant families in the world; that is why it is notoriously difficult to identify species using traditional taxonomic methods. This problem is especially apparent in the Andean region of South America, a global hot-spot for orchid diversity. Plant DNA barcoding has been proposed to help with orchid species discrimina...
Article
Full-text available
Rhus michauxii is a perennial rhizomatous shrub native to the southeastern United States that is found mainly in sunny, dry, open rocky or sandy woodlands. Moreover, it is found on ridges or river bluffs in the inner coastal plane and lower piedmont of Virginia, Georgia, and the Carolinas. Habitat conversion to agriculture, suppression of fires, an...
Article
Full-text available
Xerophyllum asphodeloides (Xerophyllaceae), known as eastern turkeybeard, is an herbaceous perennial found in eastern North America. Due to decline and destruction of its habitat, several states rank X. asphodeloides as “Imperiled” to “Critically Imperiled”. Protocols for seed cryopreservation, in vitro germination, sustainable shoot micropropagati...
Article
Full-text available
Aims Several Sarracenia species are endangered due to habitat destruction. Knowledge of their microbiomes may better inform reintroduction efforts. We characterized bacterial communities within rhizomes of three species with an emphasis on diazotrophs. Methods Microbiomes of wild and greenhouse-grown Sarracenia were characterized using cultivation...
Article
Full-text available
Single nucleotide polymorphic (SNP) markers were used to characterize the genetic structure of Symphyotrichum georgianum (Alexander) G.L. Nesom, known more commonly as the Georgia aster, from 26 populations across the species' range. This species is considered vulnerable (G3) and was a candidate for listing under the Endangered Species Act. We samp...
Article
Full-text available
Fraser fir (Abies fraseri [Pursh] Poir.) is a coniferous species native to the Southern Appalachian Mountains of the eastern United States. The species has high economic and recreational value but is vulnerable to extinction due to introduced pests and global warming. Somatic embryogenesis technology may assist in the clonal production of desired l...
Article
Full-text available
A major barrier to the commercialization of somatic embryogenesis technology in loblolly pine (Pinus taeda L.) is recalcitrance of some high-value crosses to initiate embryogenic tissue (ET) and continue early-stage somatic embryo growth. Developing initiation and multiplication media that resemble the seed environment has been shown to decrease th...
Article
Background: Habitat loss and over collection have caused North American pitcher plants to become rare, including U.S. federally endangered Sarracenia alabamensis and S. oreophila, and S. leucophylla, S. psittacina and S. purpurea spp. venosa, endangered in several states. Objective: To develop reliable seed cryopreservation protocols for endange...
Article
The shift from vegetative to embryogenic growth requires tissue to enter a radically different program of development and can be studied in vitro through the development of somatic embryos. From an applied perspective somatic embryogenesis (SE) is expected to play an important role in increasing productivity, sustainability, and uniformity of futur...
Article
Torreya taxifolia Arn., an ancient evergreen tree, is on the brink of extinction from attack by a fungal disease, recently reported to be caused by a novel isolate of Fusarium. We report the development of a somatic embryogenesis tissue culture system that can be used for cryogenic storage of T. taxifolia cultures and subsequent plant regeneration....
Article
The genus Sarracenia forms a group of carnivorous pitcher plants native to North America. Habitat destruction and overcollection have caused pitcher plants to become rare, including U.S. federally endangered S. oreophila as well as S. leucophylla and S. purpurea spp. venosa (Raf.) Wherry, both listed as endangered in several states. Protocols for i...
Article
Somatic embryogenesis (SE) has the potential to be the lowest-cost method to rapidly produce large numbers of high-value somatic seedlings with desired characteristics for plantation forestry. At least 24 of the 115-120 known Pinus species can undergo SE. Initiation for most species works best with immature megagametophytes as starting material, al...
Article
Somatic embryogenesis (SE) is expected to play an important role in the future of US forests by providing increased productivity, sustainability, and uniformity. For broad scale implementation to occur, SE technology must work with a variety of genetically diverse trees. Douglas fir (Pseudotsuga menziesii (Mirb) Franco) is the dominant tree in the...
Article
Full-text available
A major barrier to the commercialization of somatic embryogenesis technology in loblolly pine (LP, Pinus taeda L.) is recalcitrance of some high-value crosses to initiate embryogenic tissue and to continue early-stage somatic embryo growth. Developing initiation and multiplication media that resemble the seed environment may decrease this recalcitr...

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