Kurnia Agustini

• Dr. M.Sc. Apt.
• Researcher at National Research and Innovation Agency

Aerva sanguinolenta (AS), also called Sambang colok in Indonesian, is a historically utilized herbal treatment in Tawangmangu, Central Java, Indonesia. The herb is claimed to regulate menstrual cycles, inhibit tumor growth, and reduce fever. This study aimed to characterize the phytochemical profile of AS via Liquid Chromatography High-Resolution M...

This study examined the effects of ethanol and methanol as solvents on the antioxidant properties, total phenolic content (TPC), total flavonoid content (TFC), and αglucosidase inhibition in Aerva sanguinolenta samples from Solo, Indonesia. Antioxidant activity was evaluated using the DPPH assay, while the plant's chemical profile was determined th...

The development of science has found a way to prevent skin aging in menopausal women. One of the therapies is estrogen replacement therapy (17β estradiol). Skin aging in menopausal women is associated with decreased ERα expression. Leunca fruit (Solanum nigrum L) is known to contain phytoestrogens and has the potential as an antiaging agent. The pu...

This study aimed to evaluate the anti-inflammatory activity of the combination of Ardisia humilis Vahl. and Curcuma xanthorrhiza Roxb. (AC) extract in monosodium iodoacetate (MIA)-induced osteoarthritis (OA) rat model. AC was administered orally to OA rats (240, 480, and 960 mg/kg bw) for three weeks. The control and model groups comprised OA rats...

Background: Malaria remains a significant global health burden, with drug resistance posing a major challenge to its control. The emergence of resistance to antimalarial drugs represents a critical issue in malaria management, as it heightens the likelihood of morbidity and mortality associated with the disease. There is an urgent requirement for a...

Breast cancer (BC) is a multifactorial disease involving many pathways and target molecules. Multi-target therapy through multi-compound herbal medicines is an alternative strategy to treat BC. In the present study, we elucidate the molecular mechanism of Ocimum basilicum (OB) as an anticancer agent using system bioinformatic approach and investiga...

Objective: Hyperlipidemia is increasing lipids in a body that are risk factors for cardiovascular disease that increased last over 30 years. Natural products have a large portion as pharmacological agents, particularly in disease therapies. The pharmacological activity of natural product remedies has been thoroughly screened using high approaches i...

Curcuma xanthorrhiza Roxb, Phyllanthus niruri L., and Morinda citrifolia L. are Indonesian medicinal herbs used empirically as traditional therapeutics for maintaining health. The cocktail extract of these three plants (CECPM) had been developed and demonstrated immunostimulant activity in rats. This study aimed to evaluate the acute and subchronic...

Hyperglycaemia is one condition related to inflammation leading to insulin signalling impairment. This study was conducted to investigate the insulin sensitivity improvement of Sambiloto (Andrographis paniculata (Burm. f.)) Nees extract in insulin resistance-induced HepG2 (IR-HepG2) cells by stimulating insulin sensitivities and inhibiting inflamma...

Citation: Agustini K, Kusumaningrum S, Firdayani, Sulfianti A, Wink M (2023) Estrogenic activity of Bryonia dioica Jacq. through in silico and in vitro studies on pS2 gene expression in the breast cancer cell line MCF-7. Pharmacia 70(4): 951-958. https://doi. Abstract Bryonia dioica is a member of the Cucurbitaceae that produces bioactive secondary...

It is unclear how estrogen receptor beta (ER) affects prognosis in breast cancer that is in the early stages. To determine the predictive value of ER in patients with early-stage breast cancer, we conducted a systematic review and meta-analysis. We looked for studies that evaluated the ER status in breast cancer patients article between 2010 and 20...

Herbal medicines are multi-component and can exhibit synergistic effects in the treatment of diseases. Sechium edule, Syzigium polyanthum, and Curcuma xanthorrhiza have been used in traditional medicine to reduce serum lipid levels. However, the molecular mechanism was not described clearly, especially as a mixture. Thus, we performed a network pha...

Anredera cordifolia (Ten.) Stennis, also known as Binahong (B), is an Indonesian plant used to treat diabetes. The purpose of this study was to determine the best extragent for preparing Binahong extract as an antidiabetic agent using different concentrations of ethanol (50%, 70%, and 96%), labelled as BE50%, BE70%, and BE96%. An alpha-glucosidase...

Pokok Bahasan Peningkatan kualitas dan daya saing produk obat tradisional/obat herbal Indonesia perlu didampingipemerintah, terutama dari sisi kebijakan ketahanan nasional di bidang obat. Masih minimnya jumlah produk fitofarmaka yaitu obat herbal yang memenuhi persyaratan keamanan,mutu dan manfaat serta harga terjangkau. Pemerintah perlu mendoro...

The Fim-C-S typhi recombinant protein is a protein developed from the surface of the Salmonella typhi bacteria. This protein has been known to cause a good immune response. The protein has the potential as a raw material for immuno chromatography-based with gold nano particles as rapid detection kits. Various alternatives are taken to obtain pure p...

Typhoid fever caused by Salmonella typhi is an endemic disease in Asia, including Indonesia. Based on data from the World Health Organization (WHO), there are 81% per 100,000 people in Indonesia infected with typhoid. Based on this, development of typhoid detection kit that is simple, fast, specific and sensitive is still needed. One of the develop...

Salmonella typhi is gram-negative bacteria that caused typhoid fever in humans; prevention of the disease is currently through vaccination. The development of disease detection tools is also being carried out so that the detection process is faster and more accurate. In line with the development of typhoid detection devices, prior studies have mana...

Hematology Profile of Sprague-Dawley Male Rats (Rattus norvegicus)Â Aged 7 and 10 WeeksCurrently, the normal hematology profile has not been yet available for outbred rats in Indonesia. Most of the researchers use the data of hematology profile from references outside Indonesia in which the breeding environment is different. This study aimed to obt...

Labu siam (Sechium edule (Jacq.) Sw.) merupakan tanaman sayur yang dapat digunakan untuk obat tradisional dan memiliki aktivitas sebagai antikolesterol, antihipertensi, antidiabetes maupun antioksidan. Penelitian ini bertujuan untuk menetapkan mutu ekstrak buah labu siam sebagai bahan baku obat yang berasal dari bahan alam. Standardisasi ekstrak bu...

Present investigation shows that the extract of C. aeruginosa attenuates DMBA-induced spleen carcinogenesis in Wistar rats. Three-week female Wistar rats were treated with three different C. aeruginosa extract doses (CA1: 40 mg/200 g body weight, BW; CA2: 80 mg/200 g BW; CA3: 160 mg/200 g BW) and were induced with DMBA after one-week administration...

Background: Typhoid fever is caused by Salmonella typhi infection, commonly occurs in developing countries from bad sanitation and living conditions. Vaccination against the fever has been developed by means of induction of antibody through introduction of Fim-C, one of the virulence factor proteins on the cell surface of the bacterium. Recently, w...

The quality of a medicinal plant is influenced by agronomic conditions and harvesting time. Objective: This study aimed to evaluate the effect of planting method (openair (OA) and shedding house (SH)) and harvesting time (2, 4, 6 months) of Lampeni (Ardisia humilis Vahl.) toward the inhibitory activity of alpha-glucosidase. Methods: The Lampeni see...

Typhoid fever is an endemic disease in Indonesia. Prevention of typhoid fever can be done by administering vaccines. It is known that one of the raw materials for vaccines is a recombinant protein. This study aims to obtain information on the optimum conditions for purification of Salmonella typhi Fim-C recombinant protein with Ni-NTA resin as vacc...

Typhoid fever is a world health problem, with 200,000 recorded death annually in developing countries. The discovery of new drug discovery and detection methods for typhoid is still continuing. In previous research, the 31 kilo Dalton (kDa)recombinant protein Fim-C-S typhi was successfully expressed. It was also reported that the recombinant protei...

Objective: The objective of this study is to investigate the estrogenic and antiestrogenic activity of Fenugreek seeds, Trigonella foenum-graecum L. in the estrogen-dependent breast cancer cell line, MCF-7, including its effect on the expression of estrogen-dependent presenilin 2 (pS2) gene.Methods: An activity guided fractionation was carried out...

Typhoid fever is a world health problem and often occurs in developing countries, including Indonesia. In a previous study, the UNJ Salmonella team successfully isolated, cloned, expressed, and purified inclusion bodies Fim-C S. typhi recombinant protein sized 31 Kilo Dalton (KDa). Furthermore, these proteins have been used as antigen in immunogeni...

Recombinant protein Fim-C S.typhi is a potential protein that can be used as an alternative typhoid vaccine and produced on a large scale. However, before entering into a large-scale stage, laboratory optimum data on the factors that affect the production process of Fim-C Salmonella typhi proteins are required. This study aims to obtain information...

Dracaena arborea (Willd.) Link is known to be used in traditional medicine for the treatment of various diseases. Because it possesses steroidal saponins and flavonoids which display a variety of biological actions including anti-carcinogenic, it might exhibit anti-cancer effect. However, the anti-cancer effect of D. arborea is not well known. Ther...

In the past few decades, attention and research in the field of stem cell are progressing very rapidly. Hospitals in Indonesia have been using stem cells as an alternative to cure some illnesses like diabetes, heart disease, fractures and joints, dental implants, etc. Currently, adult stem cells can be obtained not only from the spinal cord and per...

Fennel fruit (Foeniculum vulgare Mill.) is one of traditional plant medicine known having effects as anti-infl ammatory, analgesic and antioxidant. It also has been reputed to increase milk secretion, promote menstruation, facilitate birth, alleviates the symptoms of the male climacteric andincrease libido. Fennel fruit contains mainly with essenti...

Hiperuresemia prevalence tends to increase in society. A combined extract of Uncaria gambir (gambir) and Caesalinia sappan (secang), had been proven to reduce blood uric acid level in vivo. This study aimed to evaluate the subchronic toxicity of this combination in male and female Sprague Dawley rat strain. Animals were randomly grouped into four g...

Indonesian medicinal herbs have so many biological activity, including anticancer activity. This assay aims to evaluate three Indonesian medicinal plants (Trigonella foenum-graecum L. (TFG), Aglaia elliptica Blume. (AE) and Foeniculum vulgare Mill. (FV)) which have potency in anticancer activity. The experiment was done by MTT assay to observe the...

Trigonella foenum-graecum (TFG) is one of medicinal plants containing several steroidal sapogenins, such as diosgenin, yamogenin, gitogenin, tigogenin and trigoneoside, and also alkaloid trigonellin and some flavonoids such as vitexin, isovitexin, orientin, isoorientin, which has many activities, such as antidiabetic, estrogenic, and also anticance...

Salmonella typhimurium, that cause typhoid fever in mice was used as a model to study typhoid fever in humans. This study is aimed to determine the immunogenicity of recombinant protein of Fim-C-S. typhimurium and specificity of antibody anti-recombinant protein of Fim-C-S. typhimurium in ddY mice. The immunogenicity studies by ELISA indicated that...

Trigonella foenum-graecum (TFG) is one of medicinal plants contains some steroidal sapogenin such as diosgenin, yamogenin, gitogenin, tigogenin and trigoneoside, also alkaloid trigonellin, which is have many activity as antidiabetic, estrogenic and also as anti cancer. This experiment was done to explore the activity of some extract of TFG on some...

Biji Klabet (Trigonella foenum-graecum L.) mengandung sejumlah sapogenin steroid, seperti diosgenin, yamogenin, tigogenin dan trigoneosida, yang diduga memiliki potensi sebagai fitoestrogen. Penelitian ini dilakukan terhadap model hewan uji tikus putih galur Wistar betina prepubertal berusia 19 hari yang belum memiliki siklus hormonal. Perlakuan se...