• Home
  • Kunal Bhattacharya
Kunal Bhattacharya

Kunal Bhattacharya
Patanjali Research Institute · Nano-Ayur BioSciences (NABS)

M.Sc. Ph.D. (Dr. rer nat.) M.B.A.

About

72
Publications
14,744
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,993
Citations
Introduction
Systemic toxicology and bioremediation of nanomaterials in human and environment.
Additional affiliations
September 2018 - August 2019
Harvard School of Public Health, Harvard University
Position
  • Researcher
September 2018 - present
Patanjali Research Institute
Position
  • Group Leader
October 2017 - August 2018
Harvard School of Public Health, Harvard University
Position
  • Research Associate
Education
September 2005 - October 2008
University of Duisburg-Essen
Field of study
  • Toxicology
July 1998 - June 2000
University of Lucknow
Field of study
  • Life Science
July 1995 - June 1998
University of Lucknow
Field of study
  • Life Science

Publications

Publications (72)
Article
Full-text available
The prevalence of nonalcoholic steatohepatitis (NASH), characterized by fatty liver, oxidative injury, and inflammation, has considerably increased in the recent years. Due to the complexity of NASH pathogenesis, compounds which can target different mechanisms and stages of NASH development are required. A robust screening model with translational...
Article
Ethnopharmacological relevance Asthma is the leading inflammatory disease of the airways with inadequate therapeutic options. ‘Malla Sindoor’ (MS) is a metal-based ethnomedicinal formulation that has been prescribed in the ancient traditional medicinal system for treating chronic inflammations. Aim of the study Here, we validated the anti-inflamma...
Article
Full-text available
Purpose: Atopic dermatitis (AD) is a chronic inflammatory disease that varies in signs and symptoms in different individuals. General symptoms include dryness of the skin, itching, and development of red to brownish-gray patches. Divya-Kayakalp-Vati (DKV) and -Oil (DKO) are Indian polyherbal compositions prescribed for treating inflammatory skin d...
Article
Background Obesity is a modern-day epidemic affecting human lifestyle worldwide and originates from high fat-content food consumption. Traditional Ayurveda-based polyherbal medicine ‘Divya-Medohar-Vati’ (DMV) has been recommended as an anti-obesity formulation. Methods In the present study, we explored the anti-obesity activity of DMV using bioche...
Article
Full-text available
Most cell culture models are static, but the cellular microenvironment in the body is dynamic. Here, we established a microfluidic-based in vitro model of human bronchial epithelial cells in which cells are stationary, but nutrient supply is dynamic, and we used this system to evaluate cellular uptake of nanoparticles. The cells were maintained in...
Article
Full-text available
Background and aim Psoriasis (Ps) is a chronic skin inflammatory disorder, that progresses to scaly-red dermal plaque formations associated with inflammation. Divya-Kayakalp-Vati (DKV) and Divya-Kayakalp-Oil (DKO) are traditional Ayurveda herbo-mineral formulations, that are prescribed for the treatment of inflammatory dermal ailments. In the prese...
Article
Full-text available
Background Pathological stress can lead to the development of cardiac hypertrophy (CH) through induction of oxidative stress and tissue remodeling. Initiation of CH is associated with increase in cardiac myocyte size, generation of reactive oxygen species and expressional changes in fetal cardiac genes. Ayurveda has prescribed several herbo-mineral...
Article
Background Frequent alcohol consumption leads to development of alcoholic liver steatosis. Onset of steatosis is caused by alcohol metabolite ‘acetaldehyde’ causing intracellular accumulation of lipid bodies due to abnormal metabolism. Livogrit is a tri-herbal Ayurveda formulation prepared using Boerhavia diffusa L., Phyllanthus niruri L., and Sola...
Article
Full-text available
Steatosis is characterized by excessive triglycerides accumulation in liver cells. Recently, application of herbal formulations has gained importance in treating complex diseases. Therefore, this study explores the efficacy of tri-herbal medicine Divya Sarva-Kalp-Kwath (SKK; brand name, Livogrit) in treating free fatty acid (FFA)-induced steatosis...
Article
Full-text available
Zebrafish is an elegant vertebrate employed to model the pathological etiologies of human maladies such as cardiac diseases. Persistent physiological stresses can induce abnormalities in heart functions such as cardiac hypertrophy (CH), which can lead to morbidity and mortality. In the present study, using zebrafish as a study model, efficacy of th...
Article
Full-text available
Divya Sarva-Kalp-Kwath (SKK) is a poly-herbal ayurvedic medicine formulated using plant extracts of Boerhavia diffusa L. (Nyctaginaceae), Phyllanthus niruri L. (Euphorbiaceae), and Solanum nigrum L. (Solanaceae), described to improve liver function and general health. In the present study, we have explored the hepatoprotective effects of SKK in ame...
Article
Full-text available
Psoriasis is a chronic inflammatory skin disease characterized by circumscribed, red, thickened plaques with overlying silvery white scales. It is associated with the release of pro-inflammatory mediators that lead to the development of edema and distress. Here we show the anti-inflammatory and anti-psoriatic efficacies of a neutraceutical sea buck...
Conference Paper
Humans are exposed to a variety of physiological and psychological stress due to the fast pace of modern society. This results in the induction of cardiac hypertrophy and associated morbidity, which under chronic conditions may lead to mortality. In the present study, using isoproterenol (ISP), a nonselective beta-androgenic agonist, cardiac hypert...
Conference Paper
Cardiac hypertrophy is the abnormal thickening of the interventricular wall due to the enlargement of cardiomyocytes along with the altered cellular sarcomeric structures. The disease is associated with several heart diseases such as hypertension, heart failure and ischemia, and valve diseases. In the present study, using isoproterenol (ISP) – a no...
Article
Full-text available
Rheumatoid arthritis (RA) is defined as a chronic autoimmune inflammatory disorder that causes damage to limb joints and progressive injuries to secondary organs. Medical practitioners prescribe Methotrexate (MTX) as standard care medicine for treating RA. However, the long-term application of MTX has shown to have adverse health-related effects. D...
Article
Engineered nanomaterials (ENMs) used in toners to improve their performance are released in the air during laser printer use. ENMs play an important catalytic role in the breakdown of the toner polymer and subsequent rearrangement of organic compounds as well as in the formation of reactive oxygen species (ROS). Cellular, animal, and human occupati...
Article
Cellulose is widely used as a thickener and filler in foods and drugs. It has been designated “generally regarded as safe” (GRAS). Nanocellulose (NC) has many additional potential applications designed to improve food quality and safety, but has not yet been designated as GRAS. Here we present results of toxicological studies of ingested NC in phys...
Article
Full-text available
Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disorder that affects joints of hands and feet and introduces injury in secondary organs such as cardiac tissue. In the present study, we induced RA in male Balb/c mice (CAIA) using collagen-antibody cocktail (C-Ab) and lipopolysaccharide intraperitoneal injections. Induction of RA in t...
Article
The rapid adoption of nanocellulose-based engineered nanomaterials (CNM) by many industries generates environmental health and safety (EHS) concerns. This work presents the development of fluorescently tagged CNM which can be used to study their interactions with biological systems. Specifically, cellulose nano-fibrils and cellulose nano-crystals w...
Book
Full-text available
The Institute of Environmental Medicine (IMM), a department at Karolinska Institutet in Sweden, has published a report on nanotoxicology. The report is a collaboration between scientists from several different units at IMM, and the work has been coordinated by professor Bengt Fadeel, head of the unit of molecular toxicology and chair of SweNanoSafe...
Article
Food matrix effects impact the bioavailability and toxicity of pharmaceuticals, nutraceuticals, pesticides, and engineered nanomaterials (ENMs). However, there are currently no standardized food models to test the impact of food matrix effects using in vitro gastrointestinal models. The purpose of this study was to establish a standardized food mod...
Article
Polyethylene glycol (PEG)-modified carbon nanotubes have been successfully employed for intra-articular delivery in mice without systemic or local toxicity. However, the fate of the delivery system itself remains to be understood. In this study 2 kDa PEG-modified single-walled carbon nanotubes (PNTs) are synthesized, and trafficking and degradation...
Article
Neutrophils were previously shown to digest oxidized carbon nanotubes through a myeloperoxidase (MPO)-dependent mechanism, and graphene oxide (GO) was found to undergo degradation when incubated with purified MPO, but there are no studies to date showing degradation of GO by neutrophils. Here we produced endotoxin-free GO by a modified Hummers’ met...
Article
Engineered nanomaterials (ENMs) are being produced for an increasing number of applications. Therefore, it is important to assess and categorize ENMs on the basis of their hazard potential. The immune system is the foremost defence against foreign bodies. Here we performed cytokine profiling of a panel of nineteen representative ENMs procured from...
Conference Paper
We used atomic force microscopy (AFM) to study the morphology and development of mineralization-competent matrix vesicles (MVs) secreted by chondrocytes isolated from WT and Phospho1−/− mice in order to validate the role of PHOSPHO1 in MV mineralization. All MVs appeared as flattened globular features either individually dispersed or connected to a...
Article
Full-text available
We report a detailed computational and experimental study of the interaction of single-walled carbon nanotubes (SWCNTs) with the drug-metabolizing cytochrome P450 enzyme, CYP3A4. Dose-dependent inhibition of CYP3A4-mediated conversion of the model compound, testosterone, to its major metabolite, 6β-hydroxy testosterone was noted. Evidence for a dir...
Article
Full-text available
We have previously shown that ablation of either the Phospho1 or Alpl gene, encoding PHOSPHO1 and tissue-nonspecific alkaline phosphatase (TNAP) respectively, lead to hyperosteoidosis but that their chondrocyte- and osteoblast-derived matrix vesicles (MVs) are able to initiate mineralization. In contrast, the double ablation of Phospho1 and Alpl co...
Article
Full-text available
Unlabelled: Carbon-based nanomaterials including carbon nanotubes, graphene oxide, fullerenes and nanodiamonds are potential candidates for various applications in medicine such as drug delivery and imaging. However, the successful translation of nanomaterials for biomedical applications is predicated on a detailed understanding of the biological...
Article
Unlabelled: Cartilage undergoes drastic structural changes during the development of osteoarthritis and cannot heal itself due to a defective chondrocyte response. Thus, much effort has been invested in the development of disease modifying drugs able to block key mediators within the cartilage matrix and biochemical pathways inside chondrocytes. H...
Article
Nanomaterials are small and the small size and corresponding large surface area of nanomaterials confers specific properties, making these materials desirable for various applications, not least in medicine. However, it is pertinent to ask whether size is the only property that matters for the desirable or detrimental effects of nanomaterials? Inde...
Article
Full-text available
Metal and metal-oxide nanoparticles are an important class of materials with numerous applications. Understanding how such nanoparticles interact with living systems is of considerable relevance both from a toxicological and biomedical perspective. The physicochemical features of nanoparticles are sometimes referred to as the synthetic identity,...
Article
Full-text available
Single-walled carbon nanotubes (SWCNTs) coated or functionalized with PEG chains of different molecular weight were assessed for their propensity to undergo biodegradation under in vitro conditions using recombinant myeloperoxidase (MPO) or ex vivo using freshly isolated primary human neutrophils. Our findings suggest that under natural conditions,...
Article
Full-text available
The composition and mode of synthesis of nanoparticles can affect interaction with bacterial and human cells differently. The present work describes the ability of β-cyclodextrin (β-CD) capped silver nanparticles to inhibit biofilm growth and reduce cytotoxicity. Biofilm formation of Staphylococcus epidermidis CSF41498 was quantified by a crystal v...
Article
Neutrophils extrude neutrophil extracellular traps (NETs) consisting of a network of chromatin decorated with antimicrobial proteins to enable non-phagocytic killing of microorganisms. Here, utilizing a model of ex vivo activated human neutrophils, we present evidence of entrapment and degradation of carboxylated single-walled carbon nanotubes (SWC...
Article
Carbon nanotubes have gained tremendous interest in a wide range of applications due to their unique physical, chemical, and electronic properties. Needless to say, close attention to the potential toxicity of carbon nanotubes is of paramount importance. Numerous studies have linked exposure of carbon nanotubes to the induction of inflammation, a c...
Article
The Policy Forum “End the deadlock on governance of geoengineering research” (E. A. Parson and D. W. Keith, 15 March, p. [1278][1]) advances proposals for governmental regulation of geoengineering, the use of technologies to alter the climate in an attempt to mitigate the impacts of global
Article
In biological systems, water takes up to 80% of the volume inside a cell. This water solubilises the biological macromolecules such as the DNA, proteins and lipids. Recent advancements have shown that the water at the interface of a lipid is structured, five layers of structured water have been found at this solvent cage. Steady state spectroscopy,...
Article
Increasing utilization of engineered nanoparticles in the field of electronics and biomedical applications demands an assessment of risk associated with deliberate or accidental exposure. Metal based nanoparticles are potentially most important of all the nanoparticles in terms of health risks. Microporous alumino-silicates and pure silicates named...
Article
Microbial colonisation on clinical and industrial surfaces is currently of global concern and silane based sol-gel coatings are being proposed as potential solutions. Sol-gels are chemically inert, stable and homogeneous and can be designed to act as a reservoir for releasing antimicrobial agents over extended time periods. In the present study, si...
Article
Full-text available
The specific properties of nanoscale particles, large surface-to-mass ratios and highly reactive surfaces, have increased their commercial application in many fields. However, the same properties are also important for the interaction and bioaccumulation of the nonbiodegradable nanoscale particles in a biological system and are a cause for concern....
Article
Full-text available
Due to its high lateral resolution, Raman microspectrsocopy is rapidly becoming an accepted technique for the subcellular imaging of single cells. Although the potential of the technique has frequently been demonstrated, many improvements have still to be realised to enhance the relevancy of the data collected. Although often employed, chemical fix...
Article
PNIPAM nanoparticles, with and without a covalently linked fluorescent label, were prepared by a free radical polymerisation technique. The cyto- and genotoxicity of PNIPAM nanoparticles were analysed in two representative mammalian cell lines, SW480, a colon, and HaCaT, a dermal cell line. Physical characterisation in terms of particle size and ze...
Article
Full-text available
Three dimensional collagen gels have been used as matrices for the imaging of live cells by Raman spectroscopy. The study is conducted on a human lung adenocarcinoma (A549) and a spontaneously immortalized human epithelial keratinocyte (HaCaT) cell line. The lateral resolution of the system has been estimated to be <1.5 μm making it possible to acc...
Article
Scitation is the online home of leading journals and conference proceedings from AIP Publishing and AIP Member Societies
Article
Full-text available
Three dimensional collagen gels are evaluated as matrices for the study of live cells by Raman spectroscopy. The study is conducted on a human lung adenocarcinoma (A549) and a spontaneously immortalized human epithelial keratinocyte (HaCaT) cell line. It is demonstrated, using the Alamar Blue assay, that both cell models exhibit enhanced viability...
Article
Full-text available
Nanomaterials are extensively used in industry and daily life, but little is known about possible health effects. An intensified research regarding toxicity of nanomaterials is urgently needed. Several studies have demonstrated that nanoparticles (NPs; diameter < 100 nm) can be transported to the central nervous system; however, interference of NPs...
Article
Full-text available
Titanium dioxide (TiO2), also known as titanium (IV) oxide or anatase, is the naturally occurring oxide of titanium. It is also one of the most commercially used form. To date, no parameter has been set for the average ambient air concentration of TiO2 nanoparticles (NP) by any regulatory agency. Previously conducted studies had established these n...