About
27
Publications
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Introduction
My research delves into understanding host-microbe interactions and bacterial cell biology during stress and differentiation using bacterial proteins and their interaction partners.
Currently, working as post-doctoral Scientist at LUH, Hannover, Germany on understanding cell biology of S. venezuelae using protein biochemistry, molecular biology, CRISPR/Cas9 and immuno- fluorescence microscopy.
Additional affiliations
July 2011 - November 2016
ICAR-National Dairy Research Institute
Position
- PhD Student
Description
- I showed proof-of-concept and the binding mechanism of a probiotics surface adhesin (mucus binding protein) to develop it as a potential nutraceutical and its efficacy in protection of human host cells from Enterotoxigenic E. coli, an important enteric pathogen.
May 2019 - June 2020
Position
- Researcher
Description
- My research involves search for novel antibiotic drug candidates against Psueodomonas aeruginosa using: • Characterization of proteins involved in P. aeruginosa infection using Protein Structural biology. • CRISPR/Cas9 based gene editing, Molecular microbiology, Biochemistry. • Galleria infection models and novel antibiotic drug candidates.
May 2017 - March 2019
Position
- PostDoc Position
Description
- This 2 year postdoctoral fellowship (NPDF) grant from Govt. of India was used to generate the first sewage microbiome of India (across 22 states) and estimate the status of antimicrobial resistance in India using next gen. sequencing and Mass spec based antibiotic estimation. My research led to generation of pan-India antibiotic resistance map, India’s first sewage microbiome and their potential as human population-level disease predictors against anemia, obesity and diabetes.
Publications
Publications (27)
Abstract Surface adhesins of pathogens and probiotics strains are implicated in mediating the binding of microbes to host. Mucus-binding protein (Mub) is unique to gut inhabiting lactic acid bacteria; however, the precise role of Mub proteins or its structural domains in host-microbial interaction is not well understood. Last two domains (Mubs5s6)...
Streptomyces are our principal source of antibiotics, which they generate concomitant with a complex developmental transition from vegetative hyphae to spores. c-di-GMP acts as a linchpin in this transition by binding and regulating the key developmental regulators, BldD and WhiG. Here we show that c-di-GMP also binds the glycogen-debranching-enzym...
Sewage wastewater pollutes water and poses a public health issue but it could also prove useful in certain research domains. Sewage is a complex niche relevant for research concerning ‘one-health’, human health, pollution and antibiotic resistance. Indian gut microbiome is also understudied due to sampling constraints and sewage could be used to ex...
Genome technologies have generated an unparalleled quantity of resources to contribute to innovation, such as environmental sustainability, food security, alternative energy sources, and human health. It allows us to identify and categorize genetic heritage, responsiveness to environmental factors, and prospective therapeutic approaches. In this ch...
Antimicrobial resistance is raging, but large size of India limits comprehensive exploration. This demands a sample like sewage, which could represent a large population and is often reported to harbor resistant microbes. Here, we did pan-India sewage sampling and studied the antibiotic resistance pattern in the microbial community. We used culture...
Here we report on the rampant state of antibiotic resistance in culturable microbes across 22 states of India and its independence with the prevailing concentration of antibiotics in the samples. This complements our recent publication on Indian sewage microbiome (https://doi.org/10.1016/j.scitotenv.2022.160178) and is important in the context that...
Probiotic surface layer proteins (Slps) have multiple functions and bacterial adhesion to host cells is one of them. The precise role of Slps in cellular adhesion is not well understood due to its low native protein yield and self-aggregative nature. Here, we report the recombinant expression and purification of biologically active Slp of Lactobaci...
The growing consumer awareness towards healthy and safe food has reformed food processing strategies. Nowadays, food processors are aiming at natural, effective, safe, and low-cost substitutes for enhancing the shelf life of food products. Milk, besides being a rich source of nutrition for infants and adults, serves as a readily available source of...
This book provides an overview of cutting-edge and next-generation research and technologies for combating antimicrobial resistance. Antibiotic-resistant infections are projected to cause 10 million deaths annually by the year 2050. A sustainable, unified approach by researchers, practitioners, policymakers, and the public is urgently needed to ste...
Sewage wastewater pollutes water and poses a public health issue but it could also prove useful in certain research domains. Sewage is a complex niche relevant for research concerning ‘one-health’, human health, pollution and antibiotic resistance. Indian gut microbiome is also understudied due to sampling constraints and sewage could be used to ex...
Staphylococcus aureus is a major pathogen of public
health concern with dominant role in food poisoning outbreaks,
nosocomial and community-acquired infections, and also in
bovine mastitis. Milk being a rich nutritious source for growth
and proliferation of pathogenic species is prone to bacterial
contamination, which can lead to spoilage and food...
Antimicrobial resistance is a global threat that kills at least 75,000 people every year worldwide and causes extended hospital stays. In the coming 10 years, antimicrobial resistance is projected to have huge health and economic burden on countries, and the scarcity of available antibiotics further worsens the situation. Antimicrobial resistance r...
Human milk is elixir for neonates and is a rich source of nutrients and beneficial microbiota required for infant growth and development. Its benefits prompted research into probing the milk components and their use as prophylactic or therapeutic agents. Culture-independent estimation of milk microbiome and high-resolution identification of milk co...
Bacteria employ many molecular mechanisms to communicate and adapt the behavior according to their surrounding environment. They produce and sense small diffusible signaling molecules called autoinducers which regulate gene expression involved in virulence,
biofilm formation, production of siderophores and protease. This mechanism of communication...
The human gastrointestinal (GI) tract has been bestowed with the most difficult task of protecting the underlying biological compartments from the resident commensal flora and the potential pathogens in transit through the GI tract. It has a unique environment in which several defense tactics are at play while maintaining homeostasis and health. Th...
The deaths caused due to drug-resistant microbes exceed 50,000 per year worldwide and antimicrobial resistance is now being considered as one of the biggest threat to human health. The emergence and spread of antimicrobial resistance warrants immediate attention from health professionals and political heads alike. The complex and multifactorial nat...
Diabetes and other lifestyle disorders have been recognized as the leading cause of morbidity and mortality globally. Nuclear factor kappa B (NF-κB) is a major factor involved in the early pathobiology of diabetes and studies reveal that hyperglycemic conditions in body leads to NF-κB mediated activation of several cytokines, chemokines and inflamm...
The history, present and future of Saccharomyces boulardii: the probiotic yeast.
Widely perceived earlier as a future medicine, Gene Therapy has progressed at a pace never before and has become today's reality. With earlier conception of limited applicability in genetic diseases only, this technique has now being explored for metabolic and physiological disorders too. More than a dozen diseases are being targeted with this appr...
