Kumar Pranaw

Kumar Pranaw
University of Warsaw | UW · Department fo Environment Microbiology and Biotechnology

Ph.D. Biotechnology

About

34
Publications
15,236
Reads
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263
Citations
Additional affiliations
February 2019 - July 2020
Jan Evangelista Purkyně University
Position
  • PostDoc Position
January 2017 - January 2019
Indian Institute of Technology Delhi
Position
  • Research Associate
October 2015 - December 2016
Criyagen Agri & Biotech Pvt. Ltd.
Position
  • Senior Researcher
Education
March 2011 - April 2012
Indian Law Institute
Indian Law Institute
Field of study
  • Intellectual Property Rights Law
March 2010 - August 2014
National Institute of Technology, Durgapur
Field of study
  • Biotechnology, Applied Microbiology
July 2006 - July 2008
VIT University
Field of study
  • Applied Microbiology

Publications

Publications (34)
Article
Full-text available
Proteases produced by Xenorhabdus are known to play a significant role in virulence leading to insect mortality. Present study was undertaken to purify and characterize protease from Xenorhabdus indica; an endosymbiant of nematode Steinernema thermophilum and deciphering its role in insect mortality and its efficacy to control Helicoverpa armigera....
Article
Full-text available
Dimethyl phthalate (DMP) a common environmental pollutant is well known for its endocrine disrupting activities. Wide spread use of the plastics and pesticides in agriculture have resulted in DMP pollution in soils. An endosymbiotic bacteria Xenorhabdus indica isolated from entomopathogenic nematode Steinernema thermophilum was investigated for bio...
Article
Full-text available
Use of plant growth-promoting bacteria (PGPB) for cultivation of the biofuel crop Miscanthus × giganteus (Mxg) in post-military and post-mining sites is a promising approach for the bioremediation of soils contaminated by metals. In the present study, PGPB were isolated from contaminated soil and screened for tolerance against abiotic stresses caus...
Article
Full-text available
The second-generation energy crop Miscanthus x giganteus (M. xgiganteus) is a perspective plant for phytor-emediation of contaminated lands and the production of biomass. The excellent quality biomass can be ensured by adding soil amendments or inoculation of the plant by plant growth-promoting bacteria (PGPB). The main goal of the current study wa...
Chapter
Climate change, environmental security, and sustainability of petrochemical resources along with depletion of fossil fuels are the current immerging issues all over the world. The industrial production of commodity chemicals from agricultural biomass provides a noteworthy contribution to solving the above mentioned environmental problems. The use o...
Article
Full-text available
The phytoremediation of industrial crops is becoming popular for the revitalization of land contaminated by trace elements (TEs). This approach combines biomass production with the improvement of soil health. To implement phytoremediation and derive sufficient dry biomass, crop production must be adequately supported by agricultural practices, incl...
Article
Full-text available
Colors with their attractive appeal have been an integral part of human lives and the easy cascade of chemical catalysis enables fast, bulk production of these synthetic colorants with low costs. However, the resulting hazardous impacts on the environment and human health has stimulated an interest in natural pigments as a safe and ecologically cle...
Article
Full-text available
Chitinases are a large and diversified category of enzymes that break down chitin, the world’s second most prevalent polymer after cellulose. GH18 is the most studied family of chitinases, even though chitinolytic enzymes come from a variety of glycosyl hydrolase (GH) families. Most of the distinct GH families, as well as the unique structural and...
Article
Full-text available
Combating the consequences of climate change is extremely important and critical in the context of feeding the world’s population. Crop simulation models have been extensively studied recently to investigate the impact of climate change on agricultural productivity and food security. Drought and salinity are major environmental stresses that cause...
Article
Lactic acid is an intermediate-volume specialty chemical, used in the production of biodegradable polymers and other chemicals. Although lactic acid production process is well established, however, the cost of production is very high. Therefore, in this study; starchy biomass (cassava) was hydrolyzed with in-house enzyme cocktail prepared from Aspe...
Article
Lignocellulosic biomass are promising renewable resources for production of biofuels and platform chemicals. The present study encompasses the screening and isolation of lactic acid bacteria (LAB), stable in the presence of ionic liquids ([EMIM][OAc], [BMIM][MeSO4], and [BMIM][Cl]) and lignocellulosic inhibitors (HMF and furfural), and explores the...
Chapter
Nanomaterials have found extensive applications in a wide array of life and industrial processes. The potential uses and benefits of nanotechnology in agriculture are important because the production of food increases with minimum cost and energy. Recently, the researcher has implemented nanomaterials in agriculture for the improvement of the crop...
Chapter
Asparaginase (E.C.3.5.1.1), an important enzyme, is broadly disseminated in all the three domains of life and responsible for catalysing conversion of asparagine into aspartic acid and ammonia. It is one of the most utilized clinical enzymes used in the treatment of different types of cancers. However, there has been a renewed interest in other app...
Article
Background: Although materials with nano-sized particles have always been prevalent in nature, lately the production and use of synthetic nanoparticles (NPs) in the industry are on the rise. This has led to a concomitant increase in their release into the environment. In the assessment of risks posed to human health it has emerged that plants are t...
Article
Full-text available
The thermophilic fungus Malbranchea cinnamomea NFCCI 3724 produced significant level of β-mannanase when grown on copra meal 131 U/gds. In solid state fermentation, parameters namely pH, moisture content and carbon supplement were optimized by response surface methodology. Optimized conditions suggested by rotatable central composite design were: p...
Article
β-glucosidase is an essential part of cellulase enzyme system for efficient and complete hydrolysis of biomass. Psychrotolerant Pseudomonas lutea BG8 produced β-glucosidase with lower temperature optima and hence can play important role in bringing down the energy requirement for bioethanol production. To enhance β-glucosidase production, two stati...
Article
The filamentous bacteria Streptomyces spp. produces diverse extracellular enzymes and other secondary metabolites. Proteomic analysis of the secretome of holocellulolytic Streptomyces sp. ssr-198 was done by tandem mass spectrometry using an Orbitrap Velos hybrid mass spectrometer. A wide range of hydrolytic enzymes, including glycoside hydrolases...
Article
Full-text available
Production of β-mannanase from thermotolerant Aspergillus terreus FBCC 1369 was optimized by response surface methodology (RSM) using plam kernel cake as substrate. Under unoptimized conditions i.e. 2 mm particle size of PKC, moisture level 1:1 and pH 6.5 fungus produced only 41 U/gds. Initial optimum conditions i.e. 0.5 particle size of PKC, locus...
Article
Full-text available
A novel thermotolerant Streptomyces sp. ssr-198 with capacity to produce multicomponent holocellulolytic enzymes was isolated and identified using 16S rDNA sequencing. Important chemical components of medium were optimized by rotatable central composite design (RCCD) using response surface methodology (RSM) to achieve maximal yield of holocelluloly...
Article
Full-text available
Xenorhabdus indica KB-3, a well-known protease producer, was isolated from its entomopathogenic nematode symbiont Steinernema thermophilum. Since medium constituents are critical to the protease production, the chemical components of the selected medium (soya casein digest broth) were optimized by rotatable central composite design (RCCD) using res...
Article
Full-text available
The objective of this study was to develop a consortium of effective microorganisms to hasten the composting process and to reduce the composting period. Results An efficient microorganism (EM) consortium was developed using Candida tropicalis (Y6), Phanerochaete chrysosporium (VV18), Streptomyces globisporous (C3), Lactobacillus sp. and enriched ph...
Conference Paper
An extracellular protease was purified and characterized from culture supernatant of Xenorhabdus indica, bacterial symbionts of soil-living entomopathogenic nematodes (EPNs), Steinernema thermophilum using ammonium sulphate precipitation and ion exchange chromatography. The enzyme was 5 fold purified with 54% yield recovery. This was further charac...
Article
Full-text available
Three bacterial strains with proteolytic, lipolytic and amylolytic activities were tested for their efficiency in enhancing the biodegradation of different organic wastes. The organic material was inoculated initially with fungal inoculant (5.0 g/kg). Bacterial consortia (1% v/w) were added after thermophilic phase and composting was carried out fo...
Article
Full-text available
Xenorhabdus sp. SGchamp, mutualistically associated with insect-pathogenic nematodes of the families Steinernematidae are known to produce two colony forms, designated as primary and secondary phase. The primary form is better in its ability to provide nutrients for the nematodes in insects as well as for entomopathogenicity of nematode. The mechan...

Questions

Question (1)
Question
Miscanthus rhizome contain how much ammount of cellulose, hemicellulose and lignin?

Projects

Projects (2)
Project
The aim of the project is to develop microbial biostimulants of crop plant growth and of biocomposting as well as biological inhibitors of phytopathogen growth. Strains selected from the culture collection of the University of Warsaw will be analyzed for their ability to survive in the soil environment (including extreme conditions, e.g. low temperature). The influence of the application of microbial strains on plant biomass, plant resistance to unfavorable environmental conditions, and phytopathogen growth in the soil will also be analyzed. In the next stage, a process manual for the immobilization of selected strains on functionalized materials and on natural carriers (e.g. zeolites) will be developed. The influence of carrier conditioning on the survival and activity of immobilized microorganisms will also be investigated. Experimental verification of biopreparations on the laboratory and pilot scales (pot experiments and micro-field/biocomposter experiments, respectively) will also be carried out. In addition, the effect of biopreparation application on the structure and biodiversity of the soil microbiome (both bacterial and fungal) will be determined.
Project
Isolates LAB or their consortium for production of lactic acid and other products from starchy and lignocellulosic biomass