K.s. Ling

K.s. Ling
United States Department of Agriculture | USDA · Agricultural Research Service (ARS)

Ph.D.

About

136
Publications
31,881
Reads
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3,284
Citations
Citations since 2017
58 Research Items
1991 Citations
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20172018201920202021202220230100200300400
20172018201920202021202220230100200300400

Publications

Publications (136)
Article
Full-text available
Tomato is an important vegetable in the United States and around the world. Recently, tomato brown rugose fruit virus (ToBRFV), an emerging tobamovirus, has impacted tomato crops worldwide and can result in fruit loss. ToBRFV causes severe symptoms, such as mosaic, puckering, and necrotic lesions on leaves; other symptoms include brown rugose and m...
Article
Full-text available
Tomato brown rugose fruit virus (ToBRFV) is an emerging tobamovirus infecting tomato and pepper crops. First identified in 2014 in the Middle East, ToBRFV has spread rapidly around the world. Being seed-borne, resistance breaking and easy mechanical transmission, ToBRFV can spread quickly in a greenhouse through plant handling. Thus, selecting an e...
Article
Full-text available
Tomato yellow leaf curl virus (TYLCV), a monopartite begomovirus in the family Geminiviridae , is efficiently transmitted by the whitefly, Bemisia tabaci , and causes serious economic losses to tomato crops around the world. TYLCV-infected tomato plants develop distinctive symptoms of yellowing and leaf upward cupping. In recent years, excellent pr...
Article
Watermelon (Citrullus lanatus) is an important vegetable fruit crop, widely grown in many countries in the tropical and subtropical regions around the world. The narrow genetic base of cultivated watermelon makes it susceptible to many pests and diseases, especially viruses. However, genetic sources of resistance are available in wild relatives tha...
Preprint
Full-text available
Tomato yellow leaf curl virus (TYLCV), a monopartite begomovirus in the family Geminiviridae , is efficiently transmitted by the whitefly, Bemisia tabaci , and causes serious economic losses to tomato crops around the world. TYLCV-infected tomato plants develop distinctive symptoms of yellowing and leaf upper cupping. In recent years, excellent pro...
Article
Tomato (Solanum lycopersicum L.) is one of the most important vegetables in the world. However, tomato is also susceptible to many viral diseases. Several tobamoviruses, including tomato mosaic virus (TMV), tomato mottle mosaic virus (ToMMV) and tomato brown rugose fruit virus (ToBRFV), are highly contagious pathogens, which could result in signifi...
Article
Full-text available
Sweet potato leaf curl virus (SPLCV) threatens global sweet potato production. SPLCV is transmitted by Bemisia tabaci or via infected vegetative planting materials; however, SPLCV was suggested to be seed transmissible, which is a characteristic that is disputed for geminiviruses. The objective of this study was to revisit the validity of seed tran...
Article
Full-text available
Background Tobamoviruses, including tomato brown rugose fruit virus (ToBRFV) on tomato and pepper, and cucumber green mottle mosaic virus (CGMMV) on cucumber and watermelon, have caused many disease outbreaks around the world in recent years. With seed-borne, mechanical transmission and resistant breaking traits, tobamoviruses pose serious threat t...
Article
Full-text available
The whitefly Bemisia tabaci (Gennadius) is a notorious insect vector that transmits hundreds of plant viruses, affecting food and fiber crops worldwide, and results in the equivalent of billions of U.S. dollars in crop loss annually. To gain a better understanding of the mechanism in virus transmission, we conducted deep sequencing of small RNAs on...
Article
Full-text available
The complete genome sequence of a U.S. isolate of a Tomato brown rugose fruit virus (ToBRFV) (CA18-01) was obtained through Illumina and MinION sequencing. The U.S. ToBRFV isolate shared a high nucleic acid sequence identity (>99%) with known ToBRFV isolates. Phylogenetic analysis revealed a tight cluster for ToBRFV isolates throughout the world, s...
Article
Full-text available
Key message A Citrullus amarus mapping population segregating for resistance to Fusarium oxysporum f. sp. niveum race 2 and Papaya ringspot virus was used to identify novel QTL, important for the improvement in watermelon disease resistance. Abstract Multiple disease screens of the USDA Citrullus spp. germplasm collection have highlighted the valu...
Article
Full-text available
Background: The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is among the 100 worst invasive species in the world. As one of the most important crop pests and virus vectors, B. tabaci causes substantial crop losses and poses a serious threat to global food security.
Article
Full-text available
Genomic decay is a common feature of intracellular bacteria that have entered into symbiosis with plant sap-feeding insects. This study of the whitefly Bemisia tabaci and two bacteria (Portiera aleyrodidarum and Hamiltonella defensa) cohoused in each host cell investigated whether the decay of Portiera metabolism genes is complemented by host and H...
Data
Genomic decay is a common feature of intracellular bacteria that have entered into symbiosis with plant sap-feeding insects. This study of the whitefly Bemisia tabaci and two bacteria (Portiera aleyrodidarum and Hamiltonella defensa) cohoused in each host cell investigated whether the decay of Portiera metabolism genes is complemented by host and H...
Article
Full-text available
Tomato chlorotic spot tospovirus is a species of the genus Orthotospovirus, family Tospoviridae. One of the causal agents of tomato spotted wilt, tomato chlorotic spot virus (TCSV) was first detected in tomato and bell pepper in south Florida in 2012 and is considered an emerging virus to the region. It has caused significant losses to tomato growe...
Article
Full-text available
Tomato spotted wilt tospovirus (TSWV), one of the most important plant viruses, causes yield losses to many crops including tomato. The current disease management for TSWV is based mainly on breeding tomato cultivars containing the Sw-5 locus. Unfortunately, several Sw-5 resistance-breaking strains of TSWV have been identified. Sw-7 is an alternati...
Article
The whitefy Bemisia tabaci, a species complex consisting of many morphologically indistinguishable species divided into distinct clades, is one of the most globally important agricultural pests and plant virus vectors. Cassava-colonizing B. tabaci transmits viruses that cause cassava mosaic disease (CMD)and cassava brown streak disease (CBSD). Half...
Article
Full-text available
Years of selection for desirable fruit quality traits in dessert watermelon (Citrullus lanatus) has resulted in a narrow genetic base in modern cultivars. Development of novel genomic and genetic resources offers great potential to expand genetic diversity and improve important traits in watermelon. Here we report a high‐quality genome sequence of...
Article
Full-text available
The whitefly Bemisia tabaci MEAM1 is a notorious vector capable of transmitting many plant viruses, resulting in serious crop loss and food shortage around the world. To investigate potential sRNA-mediated regulatory mechanisms in whiteflies that are affected by virus acquisition and transmission, we conducted small RNA (sRNA) deep sequencing and p...
Data
Distribution of piRNAs in different genomic regions. Percentage of piRNAs mapping to different genomic features. Red color denotes piRNAs derived from coding sequences (CDS), green color denotes piRNAs derived from repeat elements, and blue denotes piRNAs derived from non-coding sequences including intergenic regions, introns and UTRs. 24 h-H, 48 h...
Data
Summary of reads in 18 small RNA libraries. (DOCX)
Data
Predicted piRNAs target genes involved in diverse growth, developmental and metabolic pathways. (XLSX)
Data
piRNA clusters commonly expressed across all time points in whiteflies fed on TYLCV-infected or uninfected tomato. (XLSX)
Data
Distribution of piRNAs in different genomic regions. Representative piRNA clusters identified by the proTRAC program. It provides detailed information about the piRNA clusters including the genomic coordinates, read coverage, genes and repeat elements falling within the cluster. (TIFF)
Data
piRNAs targeting different classes of repeat elements. (XLSX)
Data
Pearson's correlation coefficients for three biological replicates and Clusters of piRNAs at each of the three different time points and treatments. (XLSX)
Data
Predicted genes targeted by suppressed piRNA clusters. (DOCX)
Data
Transposable elements targeted by suppressed piRNA clusters. (DOCX)
Article
Full-text available
Cucumber green mottle mosaic virus (CGMMV), an emerging tobamovirus, has caused serious disease outbreaks to cucurbit crops in several countries, including the United States. Although CGMMV is seed-borne, the mechanism of its transmission from a contaminated seed to germinating seedling is still not fully understood, and the most suitable seed heal...
Article
Virus-like symptoms of curled and crumpled leaves, bright yellow chlorosis, and marginal leaf necrosis typical of begomovirus infection were observed on watermelon, summer squash, and muskmelon in three counties in South Carolina in August 2017. Genus-specific primers for begomovirus-A and begomovirus-B components produced amplicons of the expected...
Article
Full-text available
The Cucurbitaceae family (cucurbit) includes several economically important crops, such as melon, cucumber, watermelon, pumpkin, squash and gourds. During the past several years, genomic and genetic data have been rapidly accumulated for cucurbits. To store, mine, analyze, integrate and disseminate these large-scale datasets and to provide a centra...
Article
Full-text available
The Agricultural Research Service (ARS) is the intramural research agency of the United States Department of Agriculture (USDA) which addresses basic scientific questions and develops applied solutions to a range of agricultural problems, and in doing so protects national food security and supports international trade. The damage to agricultural co...
Article
Full-text available
Tomato chlorotic spot orthotospovirus (TCSV) is an emerging orthotospovirus that can cause severe disease on tomato plants. There are at least four orthotospoviruses infecting tomato, and mixed infection of two or more orthotospoviruses in a single tomato plant is quite common in the field. With similarity in the symptomatology and cross serologica...
Article
Full-text available
The whitefly Bemisia tabaci can transmit hundreds of viruses to numerous agricultural crops in the world. Five genera of viruses, including Begomovirus and Crinivirus, are transmitted by B. tabaci. There is little knowledge about the genes involved in virus acquisition and transmission by whiteflies. Using a comparative transcriptomics approach, we...
Article
Bottle gourd (Lagenaria siceraria) is an important vegetable crop as well as a rootstock for other cucurbit crops. In this study, we report a high-quality 313.4-Mb genome sequence of a bottle gourd inbred line, USVL1VR-Ls, with a scaffold N50 of 8.7 Mb and the longest of 19.0 Mb. About 98.3% of the assembled scaffolds are anchored to the 11 pseudom...
Article
Full-text available
Background Whiteflies threaten agricultural crop production worldwide, are polyphagous in nature, and transmit hundreds of plant viruses. Little is known how whitefly gene expression is altered due to feeding on plants infected with a semipersistently transmitted virus. Tomato chlorosis virus (ToCV; genus Crinivirus, family Closteroviridae) is tran...
Article
Full-text available
Tomato mottle mosaic virus (ToMMV) was first identified in 2013 as a novel tobamovirus infecting tomatoes in Mexico. In just a few years, ToMMV has been identified in several countries around the world, including the United States. In the present study, we characterized the molecular, serological and biological properties of ToMMV and developed a s...
Article
Accurate detection of viruses in plants and animals is critical for agriculture production and human health. Deep sequencing and assembly of virus-derived small interfering RNAs has proven to be a highly efficient approach for virus discovery. Here we present VirusDetect, a bioinformatics pipeline that can efficiently analyze large-scale small RNA...
Article
Full-text available
Tomato planta macho viroid (TPMVd), including isolates previously designated as Mexican papita viroid (MPVd), causes serious disease on tomatoes in North America. Two predominant variants, sharing 93.8% sequence identity, incited distinct severe (MPVd-S) or mild (MPVd-M) symptoms on tomato. To identify virulence determinant factor, a series of chim...
Article
Full-text available
Background The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is among the 100 worst invasive species in the world. As one of the most important crop pests and virus vectors, B. tabaci causes substantial crop losses and poses a serious threat to global food security. Results We report the 615-Mb high-quality genome sequence of B. tabaci Middle E...
Article
Full-text available
The relationships between plant viruses and their vectors have evolved over the millennia and yet, studies on viruses began <150 years ago and investigations into the virus and vector interactions even more recently. The advent of next generation sequencing (NGS), including rapid genome and transcriptome analysis, methods for evaluation of small RN...
Article
Full-text available
The complete genome sequence of a Southern tomato virus (STV) isolate on tomato plants in a seed production field in Bangladesh was obtained for the first time using next-generation sequencing. The identified isolate, STV_BD-13, shares a high degree of sequence identity (99%) with several known STV isolates worldwide.
Article
Full-text available
The complete genome sequence of an isolate of tomato mottle mosaic virus (ToMMV) infecting tomatoes in New York was obtained using small RNA (sRNA) deep sequencing. ToMMV_NY-13 shared 99% sequence identity with isolates from Mexico and Florida. Broader distribution of this emerging virus is a cause for concern to the tomato industry.
Article
Full-text available
The complete genome sequence of Southern tomato virus (STV), a double-stranded RNA virus that affects tomato in China, was determined using small RNA deep sequencing. This Chinese isolate shares 99% sequence identity to other isolates from Mexico, France, Spain, and the United States. This is the first report of STV infecting tomatoes in Asia.
Article
Full-text available
Squash mosaic virus (SqMV), a seed-borne virus, belongs to the genus Comovirus in the subfamily Comovirinae of the family Secoviridae. SqMV has a bipartite single-stranded RNA genome (RNA1 and RNA2) encapsidated separately with two capsid proteins. With the recent identification of a third genotype in SqMV, a greater genetic diversity with only 88-...
Article
Full-text available
Genomic decay is a common feature of intracellular bacteria that have entered into symbiosis with plant sap-feeding insects. This study of the whitefly Bemisia tabaci and two bacteria (Portiera aleyrodidarum and Hamiltonella defensa) cohoused in each host cell investigated whether the decay of Portiera metabolism genes is complemented by host and H...
Article
Full-text available
Whiteflies of the Bemisia tabaci (Hemiptera: Aleyrodidae) cryptic species complex are among the most important agricultural insect pests in the world. These phloem-feeding insects can colonize over 1000 species of plants worldwide and inflict severe economic losses to crops, mainly through the transmission of pathogenic viruses. Surprisingly, there...
Article
Full-text available
The complete genome sequence (4,267 nt) of a Melon necrotic spot virus (MNSV) isolate (ABCA13-01) infecting greenhouse cucumber in Canada was determined through deep sequencing of small RNAs. Its genome sequence was most closely related to MNSV-N (97%) but lacked a 55-nucleotide insertion at the 3′ untranslated region for resistance breaking.
Article
Full-text available
The complete genome sequence (6,423 nucleotides [nt]) of an emerging cucumber green mottle mosaic virus (CGMMV) isolate on cucumber in North America was determined through deep sequencing of small (sRNA) and rapid amplification of cDNA ends. The virus shares 99% nucleotide sequence identity with the Asian genotype but only 90% with the European gen...
Article
Full-text available
The complete genome sequence of a new isolate of squash mosaic virus (SqMV) infecting squash plants in Spain was obtained using deep sequencing of small RNAs. The low nucleotide sequence identities, with only 87 to 88% for RNA1 and 84 to 86% for RNA2 to known SqMV isolates, suggested that this isolate belongs to a novel genotype.
Article
Full-text available
Background In recent years, a number of serious disease outbreaks caused by viruses and viroids on greenhouse tomatoes in North America have resulted in significant economic losses to growers. The objectives of this study were to evaluate the effectiveness of commercial disinfectants against mechanical transmission of these pathogens, and to select...
Article
Full-text available
Sweet potato leaf curl virus (SPLCV), which is transmitted by the sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), species complex, can severely affect yields of sweetpotatoes, Ipomoea batatas (L.) Lam. (Convolvulaceae). This virus is endemic in sweetpotato fields at the U.S. Vegetable Laboratory (USVL), Charleston, S.C. I...