Kristi G Bache

Kristi G Bache
Østfold University College

PhD

About

36
Publications
3,799
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
2,959
Citations
Additional affiliations
May 2018 - present
SNLA/UiO
Position
  • Editor

Publications

Publications (36)
Preprint
BACKGROUND Time spent in the prehospital phase of acute stroke care is multifactorial and have an effect on possibilities for acute treatment. Communication between paramedics and the in-hospital stroke team directly affects time to treatment. A mutual stroke scale like National Institutes of Health Stroke Scale (NIHSS) may improve communication qu...
Article
Background National Institutes of Health Stroke Scale (NIHSS) is the most validated clinical scale for stroke recognition, severity grading, and symptom monitoring in acute care and hospital settings. Numerous modified prehospital stroke scales exist, but these scales contain less clinical information and lack compatibility with in-hospital stroke...
Article
Full-text available
Background: Trauma patients have high concentrations of circulating extracellular vesicles (EVs) following injury, but the functional role of EVs in this setting is only partly deciphered. We aimed to describe in detail EV-associated procoagulant activity in individual trauma patients during the first 12 hours after injury in order to explore thei...
Article
Full-text available
Background: Acute stroke treatment in mobile stroke units (MSU) is feasible and reduces time-to-treatment, but the optimal staffing model is unknown. We wanted to explore if integrating thrombolysis of acute ischemic stroke (AIS) in an anesthesiologist-based emergency medical services (EMS) reduces time-to-treatment and is safe. Methods: A nonra...
Article
Systemic thrombolysis with tissue plasminogen activator (tPA) improves outcome in patients with acute ischemic stroke. Twenty-five years of clinical research and experience have demonstrated that the sooner tPA is administered, the better the clinical outcomes.¹ Five years ago, clinical trials demonstrating that endovascular thrombectomy can substa...
Article
Background Biomarkers indicative of intracerebral hemorrhage (ICH) may help triage acute stroke patients in the pre-hospital phase. We hypothesized that serum concentration of glial fibrillary acidic protein (GFAP) in combination with ubiquitin carboxy-terminal hydrolase-L1 (UCH-L1), measured by a rapid bio-assay, could be used to distinguish ICH f...
Article
Background: Mobile Stroke Units (MSU) diagnose and treat stroke prehospitally to reduce time from symptom onset-to-treatment (OTT). Several MSU-models exist world wide, but the best framework for MSUs are still unknown. Many models depend on in-hospital personnel working in the prehospital field. The MSU in Treat-NASPP (Norwegian Acute Stroke Preho...
Article
Background: Biomarkers indicative of intracerebral hemorrhage (ICH) may help triage acute stroke patients in the pre-hospital phase. The objective of this study was to investigate whether serum levels of glial fibrillary acidic protein (GFAP) in combination with ubiquitin carboxy-terminal hydrolase-L1 (UCH-L1) can reliably distinguish ICH from isch...
Article
Full-text available
Background: Acute ischemic stroke (AIS) is a medical emergency. The outcome is closely linked to the time elapsing from symptom onset to treatment, and seemingly small delays can mean the difference between full recovery and physical and cognitive dysfunction. Recanalization to allow blood to reenter the affected area is most efficient immediately...
Article
Background: Cerebral revascularization in acute stroke requires robust diagnostic tools close to symptom onset. The quantitative National Institute of Health Stroke Scale (NIHSS) is widely used in-hospital, whereas shorter and less specific stroke scales are used in the prehospital field. This study explored the accuracy and potential clinical ben...
Article
Full-text available
Background and purpose: In acute stroke, thromboembolism or spontaneous hemorrhage abruptly reduces blood flow to a part of the brain. To limit necrosis, rapid radiological identification of the pathological mechanism must be conducted to allow the initiation of targeted treatment. The aim of the Norwegian Acute Stroke Prehospital Project is to de...
Article
Full-text available
Contributing reviewers The editors of Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine would like to thank all our reviewers who have contributed to the journal in volume 23 (2015).
Article
Full-text available
Contributing reviewers The Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine—which received its first Impact Factor in 2013—is extremely grateful for the time, hard work and support of its highly-qualified peer reviewers. The editors of the Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, the Norwegian Air Am...
Data
FYVE-CENT and Beclin 1 expression in HCC-1395 and HCC-1954 breast cancer cells. (A) Whole cell lysates from HCC-1395 and HCC-1954 (FYVE-CENT R1945Q mutant) cell lines were analyzed by immunoblotting with the indicated antibodies. Equal amounts of cell lysates (as measured by protein content) from control and mutant cells were loaded in triplicates....
Data
Back-transfection of R1945Q FYVE-CENT mutant C terminus (1807–2539) transgene does not rescue cytokinesis arrest caused by siRNA compared to FYVE-CENT C terminus (1807–2539). Hela cells were tranfected with myc- FYVE-CENT C terminus (1807–2539) and siRNA against FYVE-CENT (A), or myc- FYVE-CENT C terminus (1807–2539) R1945Q mutant and siRNA against...
Data
Positive hits from yeast two-hybrid screening with the C-terminus of FYVE-CENT. A list of the interacting proteins with the C-terminus (residues 2120–2539) of ZFYVE26 (FYVE-CENT) were identified in a two-hybrid screen of a human T cells RP1 (CEMC7) cell library. The data are from Hybrigenics S.A, Paris, France. (XLS)
Data
A FYVE-CENT R1945Q mutant breast cancer cell line exhibits an increased number of cells arrested in cytokinesis as well as bi- and multinuclear cells. (A–B) Confocal micrographs of HCC-1395 and HCC-1954 breast cancer cells stained with the Aurora B and Hoechst (A), and HCC-1954 breast cancer cells stained with the α-tubulin, Alexa Fluor® 594 phallo...
Article
Full-text available
The tumor suppressor activity of Beclin 1 (BECN1), a subunit of class III phosphatidylinositol 3-kinase complex, has been attributed to its regulation of apoptosis and autophagy. Here, we identify FYVE-CENT (ZFYVE26), a phosphatidylinositol 3-phosphate binding protein important for cytokinesis, as a novel interacting protein of Beclin 1. A mutation...
Article
Full-text available
The endosomal sorting complexes required for transport, ESCRT-I, -II, and -III, are thought to mediate the biogenesis of multivesicular endosomes (MVEs) and endosomal sorting of ubiquitinated membrane proteins. Here, we have compared the importance of the ESCRT-I subunit tumor susceptibility gene 101 (Tsg101) and the ESCRT-III subunit hVps24/CHMP3...
Article
Alix/AIP1 is a proline-rich protein that has been implicated in apoptosis, endocytic membrane trafficking and viral budding. To further elucidate the functions of Alix, we used RNA interference to specifically suppress its expression. Depletion of Alix caused a striking redistribution of early endosomes from a peripheral to a perinuclear location....
Article
Full-text available
Ubiquitination serves as a key sorting signal in the lysosomal degradation of endocytosed receptors through the ability of ubiquitinated membrane proteins to be recognized and sorted by ubiquitin-binding proteins along the endocytic route. The ESCRT-II complex in yeast contains one such protein, Vps36, which harbors a ubiquitin-binding NZF domain a...
Article
Full-text available
The biogenesis of multivesicular bodies and endosomal sorting of membrane cargo are driven forward by the endosomal sorting complexes required for transport, ESCRT-I, -II, and -III. ESCRT-I is characterized in yeast as a complex consisting of Vps23, Vps28, and Vps37. Whereas mammalian homologues of Vps23 and Vps28 (named Tsg101 and hVps28, respecti...
Article
Full-text available
Most growth factors control cellular functions by activating specific receptor tyrosine kinases (RTKs). While overactivation of RTK signalling pathways is strongly associated with carcinogenesis, it is becoming increasingly clear that impaired deactivation of RTKs may also be a mechanism in cancer. A major deactivation pathway, receptor downregulat...
Article
Full-text available
To investigate the degradation mechanism of misfolded membrane proteins from the cell surface, we used mutant cystic fibrosis transmembrane conductance regulators (CFTRs) exhibiting conformational defects in post-Golgi compartments. Here, we show that the folding state of CFTR determines the post-endocytic trafficking of the channel. Although nativ...
Article
Full-text available
Hrs and the endosomal sorting complexes required for transport, ESCRT-I, -II, and -III, are involved in the endosomal sorting of membrane proteins into multivesicular bodies and lysosomes or vacuoles. The ESCRT complexes are also required for formation of intraluminal endosomal vesicles and for budding of certain enveloped RNA viruses such as HIV....
Article
Full-text available
STAM1 and STAM2, which have been identified as regulators of receptor signaling and trafficking, interact directly with Hrs, which mediates the endocytic sorting of ubiquitinated membrane proteins. The STAM proteins interact with the same coiled-coil domain that is involved in the targeting of Hrs to endosomes. In this work, we show that STAM1 and...
Article
The hepatocyte growth factor-regulated tyrosine kinase substrate Hrs is an early endosomal protein that is thought to play a regulatory role in the trafficking of growth factor/receptor complexes through early endosomes. Stimulation of cells with epidermal growth factor (EGF) rapidly leads to phosphorylation of Hrs, raising the question whether the...
Article
Full-text available
After endocytosis, some membrane proteins recycle from early endosomes to the plasma membrane whereas others are transported to late endosomes and lysosomes for degradation. Conjugation with the small polypeptide ubiquitin is a signal for lysosomal sorting. Here we show that the hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, is...
Article
Full-text available
The hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, has been implicated in intracellular trafficking and signal transduction. Hrs contains a phosphatidylinositol 3-phosphate-binding FYVE domain that contributes to its endosomal targeting. Here we show that Hrs and EEA1, a FYVE domain protein involved in endocytic membrane fusion,...
Article
Full-text available
The hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, becomes tyrosine-phosphorylated upon the binding of various growth factors and cytokines to their receptors. This protein is essential for ventral folding morphogenesis, and it shares structural similarity with Vps27p, which is involved in vacuolar protein sorting in yeast. Sinc...