
Krishnakant BhargavaBarkatullah University | BU · CRIM -Management Institute
Krishnakant Bhargava
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Publications (818)
Hepatic stellate cells (HSCs) are major contributors to liver fibrosis, as hepatic injuries may cause their transdifferentiation into myofibroblast-like cells capable of producing excessive extracellular matrix proteins. Also, HSCs can modulate engraftment of transplanted hepatocytes and contribute to liver regeneration. Therefore, understanding th...
To protect bone marrow from over-irradiation, maximum permissible activity (MPA) of iodine-131 ((131)I) to treat thyroid cancer is that which limits absorbed dose to blood (as a surrogate of marrow) to < 200 cGy. The conventional approach (Method-1) requires repeated gamma-camera whole-body measurements along with blood samples. We sought to determ...
Background Raised blood pressure is an important risk factor for cardiovascular diseases and chronic kidney disease. We estimated worldwide trends in mean systolic and mean diastolic blood pressure, and the prevalence of, and number of people with, raised blood pressure, defined as systolic blood pressure of 140 mm Hg or higher or diastolic blood p...
Being taller is associated with enhanced longevity, and higher education and earnings. We reanalysed 1472 population-based studies, with measurement of height on more than 18.6 million participants to estimate mean height for people born between 1896 and 1996 in 200 countries. The largest gain in adult height over the past century has occurred in S...
Kupffer cells (KC) play major roles in immunity and tissue injury or repair. Because recapitulation of KC biology and function within liver will allow superior insights into their functional repertoire, we studied the efficacy of the cell transplantation approach for this purpose. Mouse KC were isolated from donor livers, characterized, and transpl...
Background & objectives:
Abnormal endothelial function represents a preclinical marker of atherosclerosis. This study was conducted to evaluate associations between anthropometry, cardiometabolic risk factors, and early life factors and adult measures of endothelial function in a young urban Indian cohort free of clinical cardiovascular disease.
M...
Identification by molecular imaging of key processes in handling of transition state metals, such as copper (Cu), will be of considerable clinical value. For instance, the ability to diagnose Wilson’s disease with molecular imaging by identifying copper excretion in an ATP7B-dependent manner will be very significant. To develop highly effective dia...
Xanthogranulomatous cholecystitis is a chronic inflammatory reaction of the gall bladder. Its importance lies in the difficulty frequently encountered in differentiating it from carcinoma of the gall bladder . Both presents with thickening of the gall bladder wall and the imaging appearances of both entities can be quite similar. However , a close...
Performance in intelligence tests tends to be higher among individuals breastfed as infants, but little is known about the association between breastfeeding and achieved schooling. We assessed the association of infant feeding with school achievement in five cohorts from low- and middle-income countries. Unlike high-income country settings where mo...
Unlabelled:
To optimize strategies for liver-directed cell therapy, prevention of initial transplanted cell losses is particularly important for subsequent liver repopulation. After cell transplantation in hepatic sinusoids, perturbations in hepatic microcirculation along with changes in various liver cell types are among the earliest changes. The...
Sonography is increasingly being used for diagnosing a large number of musculoskeletal disorders. Its multiple advantages include low cost, accessibility, portability and non-invasiveness. But perhaps, it the unique capabilities of real time imaging and dynamic evaluation which give it a distinct edge over other diagnostic modalities. This dynamic...
A brief review of the different imaging modalities in obstructive jaundice with the relative advantages and disadvantages are described. Comparison of the different diagnostic modalities available in obstructive jaundice based on available literature. Role of non -invasive imaging modality like ultrasound which serves as a preliminary investigation...
Congenital clubfoot is a common foot deformity reported with a prevalence of 4-7/ 1000 in various populations world over. It has four component deformities in seven possible combinations. Although, the assessment is done clinically by palpation but this clinical grading of the deformity is fraught with interobserver variability and inaccuracy due t...
In clinical practice, palpation of any organ remains the main examination to determine the consistency of the organ andthus plan for future investigation. However,it has got limitation in terms of its accessibility to deeper organs as well as its subjectivity. "palpation by imaging" i.e. Elastography is a new promising technique to determine the na...
In this pilot study, an exposure–response assessment (aged 16 to 60 years, n = 1,012) was carried out related to eye problems due to vehicular fumes during the winter of 2003. Inhalable particles <PM10 and Pb level were monitored at 12 locations of Lucknow using a Respirable Dust Sampler and EPM 2000 filter. The range of PM10 particles varied from...
Excretion of copper into bile requires the copper transporter Atp7b, which is deficient in Wilson disease. We hypothesized that a radiocopper-histidine complex would be effective for diagnosing Wilson disease by molecular imaging and tested this hypothesis in the Long-Evans cinnamon (LEC) rat model with Atp7b deficiency.
We complexed (64)Cu to l-hi...
PURPOSE
Morphine, an extensively used opiate analgesic, has both pharmacological and pathological effects. Morphine is a cationic anion and it may, therefore, affect renal tubular secretion. This study was designed to investigate the effects of morphine on 99mTc-MAG3, which is cleared by tubular secretion.
METHOD AND MATERIALS
Nine mice (ages: 8-1...
The presence of histamine, 5-hydroxytryptamine (5-HT), and acetylcholine has been demonstrated in the Indian stinging nettle, Urtica parviflora (Roxb.). In addition, the presence of a histamine-liberating substance is strongly indicated. The whole leaves of the plant were extracted in acetone. The acetone-extracted material was used to detect 5-HT...
The present study deals with the quantitative effect of vehicular emission on ambient air quality during May 2006 in urban area of Lucknow city. In this study SPM, RSPM, SO2, NOx and 7 trace metals associated with RSPM were estimated at 10 representative locations in urban area and one village area for control. Beside this, air quality index (AQI),...
The present investigation deals with the accumulation of heavy metals in fields contaminated with fly ash from a thermal power plant and subsequent uptake in different parts of naturally grown plants. Results revealed that in the contaminated site, the mean level of all the metals (Cd, Zn, Cr, Pb, Cu, Ni, Mn and Fe) in soil and different parts (roo...
The present investigation deals with the accumulation of heavy metals in fields contaminated with fly ash from a thermal power plant and subsequent uptake in different parts of naturally grown plants. Results revealed that in the contaminated site, the mean level of all the metals (Cd, Zn, Cr, Pb, Cu, Ni, Mn and Fe) in soil and different parts (roo...
A 32-year old female patient presented with the tumor of the oral cavity at the Kidwai Memorial Institute of Oncology, Bangalore, India. Clinical examination and histopathological analyses confirmed the neoplasm as malignant melanoma. This rare melanoma of the buccal mucosa expressed carcinoembryonic antigen (CEA) as well as contained cells that ex...
Mature ovarian dermoid cysts are common lesions, accounting for up to 10-25% of all ovarian neoplasms. Uncomplicated dermoid cysts are often asymptomatic and are relatively easy to diagnose on imaging and to treat. Symptoms develop once complications set in and these may cause diagnostic dilemmas. Torsion (16%) is the most common complication, whil...
Thymosin β9, a 41 residue thymic polypeptide, has been synthesized by a solid phase method. A modification of the low HF method was used to deprotect and cleave the peptide from the resin. Thymosin β9 was then obtained in analytically pure form by a one-step purification procedure in 32% yield. The activity of thymosin β9 in the terminal deoxynucle...
People burn crackers world over on different occasions in different countries to express their happiness. Fireworks in large amounts aggravate the level of air pollutants and cause significant short-term air quality degradation with possible impact on human health. Fine particles (PM2.5 < or = 2.5 microm), which may pose detrimental effects on huma...
We investigated labeling human leukocytes [white blood cells (WBCs)] in vitro with copper-64 (Cu) comparing labeling efficiency, viability and stability of Cu-WBCs with (111)In-oxine (In) WBCs and (18)F-FDG (FDG) WBCs.
Leukocytes from 10 volunteers were labeled with Cu, In and FDG. Forty milliliters of venous blood was collected and leukocyte separ...
Unlabelled:
The organic anion (99m)Tc-N-[2-[(3-bromo-2,4,6-trimethylphenyl)-amino]-2-oxoethyl]-N-(carboxymethyl)-glycine ((99m)Tc-mebrofenin) and its analogs are widely used for hepatobiliary imaging. Identification of the mechanisms directing bile canalicular transport of these agents will provide insights into the basis of their hepatic handling...
The present study was directed on the assessment of the response of treatment-naive oral cancer cells to methotrexate (MTX) in vitro: and clinical response to MTX therapy.
A pilot study of in vitro: evaluation of MTX response of oral cancer cells from 10 patients was conducted using a cell viability assay to determine the sensitivity/resistance to...
Retained surgical textile is an infrequent but serious complication of abdominal surgery. Diagnosis is often delayed owing to non-specific clinical symptoms and inconclusive imaging features. Medicolegal implications further complicate the issue. We report a case of a 30-year-old woman who had previously undergone caesarean section and who presente...
Gastric volvulus is a rare entity, which when acute can be potentially life threatening. On the other hand, chronic volvulus may be detected incidentally or may result in intermittent epigastric pain and abdominal fullness following meals. Organo axial variety is twice as common than mesenteroaxial variety. We report a case of an uncommon mesentero...
The Condensation of 3,4-disubstituted phenylethylamine and benzaldehyde furnished l-phenyl-6,7-disubstituted-1,2,3,4-tetrahydroisoquinolines l. which on reaction with 1,3-dibromopropane gave l-phenyl-6,7-disubstituted-2-(3-bromoprophyl)-1,2,3,4-tetrahydroisoquinolines 2. The reaction of 2 with different secondary amines resulted in the synthesis of...
Abstract Withania somnifera (L.) Dunal was evaluated for its tumor-preventing activity against urethane-induced lung adenomas in adult male albino mice. Administration of urethane in a dose of 125 mg/kg given subcutaneously biweekly for 7 months induced lung adenomas in 100% of animals. Urethane was also found to cause significant decrease in body...
A bridge modified 4-tert-butylthiacalix[4]arene (I) has been employed as electroactive material in the preparation of cobalt selective sensor. Polyvinyl chloride (PVC)-based membranes of (I) using sodium tetraphenylborate (NaTPB) as anion discriminator and bis(2-ethylhexyl) sebacate (DOS), chloronaphthalene (CN), tri-n-butylphosphate (TBP), o-nitro...
Imaging agents capable of providing cell compartment-specific information will facilitate studies of pathophysiological mechanisms, natural history of diseases, and therapeutic development. To demonstrate the effects of liver injury on the disposal of the organic anion mebrofenin, we performed animal studies.
Acute liver injury was induced in Fisch...
Mammographic density is a function of abundance of epithelial and connective tissue in breast. It has been identified as an independent risk factor for breast cancer in studies in western populations. We conducted a case control study to evaluate the role of mammographic density as risk factor for the development of breast cancer in Indian patients...
Forty patients with variceal bleeding due to non-cirrhotic portal fibrosis have been treated by repeated endoscopic injection sclerotherapy using intravariceal polidocanol (1%). This has decreased re-bleeding as evidenced by a decrease in mean bleeding risk factor (BRF) and transfusion requirement; difference between presclerotherapy and postsclero...
The present study deals with the effect of fireworks on ambient air quality during Diwali Festival in Lucknow City. In this study, PM10, SO2, NOx
and 10 trace metals associated with PM10 were estimated at four representative locations, during day and night times for Pre Diwali (day before Diwali) and Diwali day. On Diwali day 24 h average concentra...
Urban populations are exposed to a high level of fine and ultrafine particles from motor vehicle emissions which affect human health. To assess the hourly variation of fine particle (PM2.5) concentration and the influence of temperature and relative humidity (RH) on the ambient air of Lucknow city, monitoring of PM2.5 along with temperature and RH...
The present study deals with the assessment of ambient air quality with respect to respirable suspended particulate matter (RSPM or PM10 < or = 10 microm) and trace metals (Fe, Zn, Cu, Cr Ni, Cd, Mn and Pb) concentrations in RSPM at five locations of Renukoot, an industrial area of Eastern Uttar Pradesh. The 24 hr mean concentrations of PM10 ranged...
The sonographic spectrum of abdominal tuberculosis was studied in twenty seven proven cases. The diagnosis was based on the ultrasound (US) guided FNAC, laparoscopy and histopathology and response to antitubercular chemotherapy in patients with strong clinical suspicion of abdominal tuberculosis. Tubercular lymphadenopathy was the most common manif...
The objectives of this retrospective investigation were to determine the accuracy of 99mTc-fanolesomab, an antigranulocyte antibody, for diagnosing prosthetic vascular graft infection, ascertain optimum imaging times for this indication, and assess safety of this agent.
Eighteen patients with 19 prosthetic vascular grafts were included. Indications...
Disruption of the hepatic endothelial barrier or Kupffer cell function facilitates transplanted cell engraftment in the liver. To determine whether these mechanisms could be activated simultaneously, we studied the effects of monocrotaline, a pyrollizidine alkaloid, with reported toxicity in liver sinusoidal endothelial cells and Kupffer cells. The...
Renal involvement by pancreatitis is uncommon. Pseudocysts in the kidneys carry the potential for massive hemorrhage if they erode the renal vessels or can cause thrombosis due to vascular compression. They are not as benign as simple cysts and need timely management, hence we report a case of renal pseudocyst that simulated as a simple cortical cy...
Expression of the oncofoetal glycoprotein, carcinoembryonic antigen (CEA), has been observed in a number of malignancies and is also being pursued as a target for anti-cancer therapy. This study explored the status of this biochemical entity in the oral squamous cell carcinoma (SCC) in South India caused by extensive chewing habits. Squamous cell c...
Automobiles, construction, festivals, factories, stations, diesel shades, garages and workshops are sources of noise. Vehicles are increasing enormously beyond the carrying capacity of the road. Study was carried out at 12 locations with sound level meter to assess day time and night time noise levels of Lucknow city. In residential areas, noise ra...
Filarial dance in breast lesions is a very rare entity. It is difficult to show the characteristic vigorous movement of the adult worms on static images although pulse wave Doppler and colour power Doppler have been used. Filarial dance on colour Doppler imaging has not been reported earlier to the best of our knowledge.
To compare prospectively the accuracy of positron emission tomography (PET) with leukocytes labeled in vitro with (18)F fluorodeoxyglucose (FDG) versus that of conventional scintigraphy with leukocytes labeled in vitro with (111)In oxine in patients suspected of having infection.
This HIPAA-compliant study had institutional review board approval; i...
The present investigation was undertaken to explore the ulcer-healing property of 1% amiloride ointment on mechanically produced skin ulcers in albino rabbits and at donor site of patients requiring split skin graft. Four skin ulcers measuring 2 X 2 cm(2) (two on either side of the midline) were made 2 cm apart on the pre-shaved back of each anesth...
Radiology plays an important role in the diagnosis of pulmonary tuberculosis. Many medical practitioners
believe in and rely primarily on the chest x-ray for the diagnosis of pulmonary tuberculosis. This study
attempts to evaluate the reliability and validity of chest radiographs. This study was carried out in the
tuberculosis clinic of BPKIHS. 75...
Targeting of cells to specific tissues is critical for cell therapy. To study endothelial cell targeting, we isolated mouse liver sinusoidal endothelial cells (LSEC) and examined cell biodistributions in animals. To identify transplanted LSEC in tissues, we labeled cells metabolically with DiI-conjugated acetylated low density lipoprotein particles...
Barium enema studies are considered essential for the diagnosis of tubular colonic duplication with opacification of two colons being the diagnostic sign. Yet in certain cases, barium enema apparently does not show positive findings. A case is reported in which a barium follow-through study established the complete diagnosis of duplication of colon...
Background: South Asians have a muscle-thin but adipose body phenotype and high rates of obesity-related disease. Adult body composition may be predictable in early life. Objective: Anthropometric indexes of adult body composition were examined in relation to birth size and body mass index (BMI) during childhood. Design: A population-based cohort o...
A prospective study was conducted to assess the role of sonography in ocular diagnosis. Theirty six (36) patients with suspected ocular disease were examined using non-dedicated ultrasound scanners by small part probe. Sonographic (US) findings were correlated with clinical course of disease, surgical or histopathological findings. Amongst 29 patie...
Many diseases are associated with cytokine release after inflammatory infiltration, which perturbs organ function. Radioligands capable of noninvasive tracking to assess the integrity of specific biochemical pathways offer potent ways to establish such perturbing mechanisms.
To demonstrate regulation of hepatobiliary transport in disease, we used (...
The objectives of this study were to investigate (18)F-FDG imaging, using a coincidence detection system, for diagnosing prosthetic joint infection and to compare it with combined (111)In-labeled leukocyte/(99m)Tc-sulfur colloid marrow imaging in patients with failed lower extremity joint replacements.
Fifty-nine patients--with painful, failed, low...
Exophytic adenocarcinoma of the stomach (EAS) is a tumor that has a large extraluminal portion. Exophytic growth of gastric carcinoma is rare (1), and such tumor may be confused with malignant stromal tumor (MST). We report the US findings of adenocarcinoma with an exophytic component and its differentiating features from malignant stromal tumor.
Garlic oil was evaluated for gastroprotective activity against ethanol induced ulcers. Reactive oxygen species are involved in the pathogenesis of these ulcers. The possible involvement of garlic oil in restraining the oxidation process produced in gastric tissue was also investigated. The ulcer index, lipid peroxidation and antioxidant enzyme acti...
Omeprazole is frequently used in patients with cirrhosis of the liver to treat peptic ulcer disease. It is also used for the healing of mucosal lesions after endoscopic sclerotherapy of esophageal varices in cirrhosis and extraheptic portal vein obstruction (EHPVO). This study was carried out with the aim of determining the pharmacokinetics of omep...
Aspirin is widely used as an antiplatelet agent in the primary and secondary prevention of cardiovascular disease. In order to spare prostacyclin formation and reduce gastrointestinal side-effects, very low doses of aspirin have been introduced. However, it remains unclear whether these low doses are equally effective with respect to inhibition of...
Calcium channel antagonists have been shown to have an anticonvulsant activity in a variety of seizure models and also to potentiate the anticonvulsant activity of other standard antiepileptic drugs like carbamazepine, phenytoin and valporoate. A pharmacokinetic interaction may be involved in such potentiation. This cross over single dose study was...
Technetium-99m sestamibi has attracted interest for assessment of the function of P-glycoproteins, which are well expressed in the liver and have roles in biliary transport and the removal of chemotherapeutic drugs. To further examine the cross-reactivity of (99m)Tc-sestamibi for P-glycoprotein family members, we conducted studies in animals. Hepat...
Accurate interpretation of labeled leukocyte images requires knowledge of pulmonary labeled leukocyte uptake: its prevalence and patterns and its correlation with technical, physiologic, and pathologic conditions as well as with other imaging findings. Images obtained shortly after injection of labeled cells are characterized by diffuse pulmonary a...
Kupffer cells are activated shortly after deposition of hepatocytes in liver sinusoids, with clearance of a significant fraction of transplanted cells, especially when cells are entrapped in portal spaces. We determined whether perturbation of Kupffer cells would improve transplanted cell engraftment.
Dipeptidyl peptidase IV-deficient rats were use...
The present study was done to investigate the neurotransmitter mechanisms involved in gabapentin antinociception in healthy albino rats. The formalin test was used to asses antinociception. Gabapentin (10-120 mg/kg s.c.) decreased the pain score in the formalin test. In order to study the putative neurotransmitter mechanisms involved in gabapentin...
To determine whether disruption of the hepatic sinusoidal endothelium will facilitate engraftment of transplanted cells, we treated Fischer 344 (F344) rats lacking dipeptidyl peptidase IV (DPPIV) activity with cyclophosphamide (CP). Electron microscopy showed endothelial injury within 6 hours following CP, and, after 24 and 48 hours, the endotheliu...
To determine if there is a difference in early relapse rates and adrenal suppression between patients receiving an 8-day course of 40 mg/day prednisolone and those receiving an 8-day tapering course of prednisolone.
This was a prospective, randomized, open clinical trial conducted in a tertiary care center. All asthmatic patients with exacerbation...
After transplantation, hepatocytes entering liver sinusoids are engrafted, whereas cells entrapped in portal spaces are cleared. We studied whether hepatic sinusoidal dilatation will increase the entry of transplanted cells in the liver lobule, improve cell engraftment, and decrease microcirculatory perturbations. F344 rat hepatocytes were transpla...
The study was conducted to examine the role of free radicals in Indomethacin induced gastric mucosal injury and to evaluate the gastroprotective effects of melatonin and beta-carotene. Gastric mucosal injury was produced in rats by administering indomethacin 30 mg/kg subcutaneously. Melatonin was administered in three different doses of 5, 10 and 2...
To evaluate the involvement of Ca++ channels in Gabapentin antinociception. In healthy male albino rats formalin (50 microliters, 5%) was injected at the planter surface of the paw. Pain score was calculated. Antinociceptive effect of (10, 30 mg/kg) gabapentin and (3, 10 mg/kg) nifedipine alone and on co-administration of sub analgesic doses was st...
We studied sixty patients (age 2-12 years) of presumed idiopathic seizure dissorders. All the cases presenting with partial seizures generalized, seizures neurological findings, focal paroxysmal discharges and showing electrocephato graphic changes were initially screened with a detailed history, clinical examination; were later subjected to a CT s...
Bacterial infections are common in patients with cirrhosis of liver and are frequently treated with ciprofloxacin. Literature on pharmacokinetics of ciprofloxacin in patients with cirrhosis of the liver is scanty. The present study compared the pharmacokinetics of ciprofloxacin in cirrhotic patients with that in healthy volunteers.
In 20 patients w...
The purpose of this study was to establish whether (99m)Tc-mebrofenin could noninvasively assess liver function in Wilson's disease.
Long-Evans Cinnamon (LEC) rats, which reproduce Wilson's disease with copper toxicosis, and their normal counterparts, Long-Evans Agouti (LEA) rats, were studied. Scintigraphic findings were correlated with biliary me...
Until recently, noise pollution has not been paid adequate attention as air, water and land pollution. In order to assess (predict) the impact of bauxite mine noise on employees health and in and around bauxite mine environment, general noise sources and equipment noise were monitored. All these noise sources were compared with prescribed standard...