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Working in the lab of Dr. Bryan F Singer exploring rat affective state during cocaine and heroin self-administration.
February 2020 - present
- Doctoral tutor
- Doctoral tutor for the modules (past and current): - Psychology of Childhood - Developmental Psychology - Introduction to Neuroscience he role involves : - Hosting team-based learning workshops designed to provide students with an opportunity to discuss material they learned in lectures and independent reading. Students are then encouraged to apply the discussed material to solve real-life problems. - Running assessment clinics to providing students with support relating to their assessments.
September 2018 - present
- I am working in the labs of Prof Aldo Badiani and Dr Bryan F. Singer investigating simultaneous cocaine and heroin self-administration, ultrasonic vocalizations, and behavioural economics and drug preference in rats. Techniques include: - Rat handling - Implantation of double-lumen catheters into the jugular vein of rats - Self-administration experiments - Ultrasonic vocalization recording
October 2017 - February 2018
- Research Assistant
- This position was in the lab of Dr Stephan de la Rosa at the Department of Human Perception, Cognition & Action, Max Planck Institute for Biological Cybernetics, Tuebingen, Germany. The project investigated colour adaptation in the visual periphery. The role involved the recruitment of participants and the collection of psychophysical data.
Rationale Interoception is the signalling, perception, and interpretation of internal physiological states. Many mental disorders associated with changes of interoception, including depressive and anxiety disorders, are treated with selective serotonin reuptake inhibitors (SSRIs). However, the causative link between SSRIs and interoception is not y...
Interoception is the signaling, perception, and interpretation of internal physiological states. A causal link between serotonin and interoception was tested by a within-participant, crossover, placebo-controlled study. Forty-seven healthy human volunteers were tested on and off a 20mg oral dose of the selective serotonin reuptake inhibitor (SSRI)...
I am currently embedding intestine and liver samples into OCT and was wondering if there is any technique to avoiding bubbles appearing in the OCT solution.
I have isolated RNA from 12 mouse samples and analyzed expression of 18S with real time quantitative PCR.
Half of the mice show ideal CP values between 15 - 25 cycles, whereas the other half produce CP values of around 32 cycles.
Electrophoresis analysis shows that all samples have intact RNA, with clear 28S and 18S bands visible. Nanodrop analysis gives good A260/280 ratios between 2.0-2.1 and A260/230 ratios of 2.0-2.2
Does anybody have any suggestions for what could be causing CP values to occur later for only half of the mice tested despite all mice showing the same electrophoresis and nanodrop results?
I am preparing mouse intestinal RNA samples from which I will use RT-PCR to produce cDNA and consequently perform qPCR on this cDNA.
My question is, from these electrophoresis results (attached), is my RNA of good enough quality for qPCR?