Krasavin Eugene

Krasavin Eugene
Joint Institute for Nuclear Research | jinr ОИЯИ · Laboratory of Radiation Biology

Dr. Sci., Professor

About

181
Publications
14,215
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1,281
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Introduction
Skills and Expertise
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June 2005 - October 2015
Joint Institute for Nuclear Research
Position
  • Managing Director

Publications

Publications (181)
Patent
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Способ повышения частоты образования двунитевых разрывов ДНК в клетках человека при действии ионизирующих излучений в условиях влияния радиомодификаторов, отличающийся тем, что в качестве ингибиторов репарации ДНК перед облучением применяют комбинацию из официнальных препаратов 1-β-D-арабинофуранозилцитозина (цитарабина) и гидроксимочевины
Article
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The aim of this research was to study behavioral reactions and morphological changes in the brain of adult female Sprague Dawley rats after exposure to 170 MeV and 70 MeV protons and gamma radiation (60Co) at a dose of 1 Gy. The analysis of the behavioral reactions in the T-maze showed that exposure to ionizing radiation with different LETs led to...
Article
Full-text available
Di-glycosylated adenines act as glycosyl donors in the intermolecular trans-glycosylation of pyrimidine nucleobases under proton beam irradiation conditions. Formamide and chondrite meteorite NWA 1465 increased the yield and the selectivity of the reaction. The glycosyl transfer process was highly regioselective in yielding canonical N 1-pyrimidine...
Article
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In a study on primates (Macaca mulatta), neurobiological and radiobiological effects have been studied of the synchronous combined action of 7-day antiorthostatic hypokinesia and exposure of the monkeys' head first to γ-rays during 24 h and then to accelerated ¹²C ions. The neurobiological effects were evaluated by the cognitive functions which mod...
Article
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The radiobiological effects of accelerated ions with high charge and high energy (HZE) on mammalian cells and their propagation in time are still not sufficiently explained and attract great deal of attention. This work aims to compare the immediate and delayed effects with emphasis on the latter. As shown by our group, the dependence of mutant fra...
Article
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High-LET (Linear Energy Transfer) particle irradiation as being provided from heavy ion accelerator facilities has an increasing impact on bio-medical research and cancer treatment. Nevertheless, there are a lot of open questions concerning the understanding of damaging mechanisms and repair processes within the light of radio-sensitivity and thus,...
Book
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The results of thorough bacterialpaleontological study of the Orgueil meteorite are presented in this illustrated Atlas of microfossils (both prokaryotic and eukaryotic) found within freshly fractured interior surfaces of the meteorite. More than 60 images obtained with Scanning Electron Microscope are presented.
Article
Formation and structural modification of oxygenated polycyclic aromatic hydrocarbons (oxyPAHs) by UV irradiation on minerals have recently been proposed as a possible channel of PAH transformation in astrochemical and prebiotic scenarios of possible relevance for the origin of life. Herein, we demonstrate that high energy proton beam irradiation in...
Article
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Sphingomyelins (SMs) are a class of relevant bioactive molecules that act as key modulators of different cellular processes, such as growth arrest, exosome formation, and the inflammatory response influenced by many environmental conditions, leading to pyroptosis, a form of programmed cell death due to Caspase-1 involvement. To study liver pyroptos...
Article
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This study is based on our already published experimental data (Kowalska et al. in Radiat Environ Biophys 58:99–108, 2019) and is devoted to modeling of chromosome aberrations in human lymphocytes induced by 22.1 MeV/u ¹¹B ions, 199 MeV/u ¹²C ions, 150 MeV and spread-out Bragg peak (SOBP) proton beams as well as by ⁶⁰Co γ rays. The curvature of the...
Article
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In the present work, we have adopted the premature chromosome condensation (PCC) technique to study the initial DNA damage in order to distinguish between the biological and physical components of the dose-effect curves. We have studied G 2 -chromatid breaks in human lymphocytes, isochromatid-type breaks as well as chromatid exchanges at two differ...
Article
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Studies on the relationship between reactive oxygen species (ROS)/manganese superoxide dismutase (MnSOD) and sphingomyelinase (SMase) are controversial. It has been demonstrated that SMase increases the intracellular ROS level and induces gene expression for MnSOD protein. On the other hand, some authors showed that ROS modulate the activation of S...
Article
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The abiotic phosphorylation of nucleosides is a major hurdle in origin‐of‐life studies. We suggest a plausible pathway for the synthesis of adenosine nucleotides from adenosine and NaH2PO4 under radiative conditions mimicking the solar wind in the presence of a meteorite of the aubrite‐type. Hydroxyapatite also performed as a mineral heterogeneous...
Article
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The induction and repair of DNA double-strand breaks in hippocampal formation after exposure to accelerated 78Kr ions with an energy of 2.58 GeV/nucleon has been studied using the immunohistochemical staining of rat-brain paraffin sections with antibodies specific to γH2AX and 53BP1 proteins. Following exposure to heavy ions, complex clusters of do...
Article
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The formation of nucleosides in abiotic conditions is a major hurdle in origin-of-life studies. We have determined the mechanism of a general reaction leading to the one-pot synthesis of ribo- and 2’-deoxy-ribonucleosides from sugars and purine nucleobases under proton irradiation in the presence of a chondrite meteorite. These conditions simulate...
Article
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We investigated induction of chromosome aberrations (CA) in human lymphocytes when exposed to 150 MeV and spread out Bragg peak (SOBP) proton beams, and 199 MeV/u carbon beam which are currently widely used for cancer treatment and simultaneously are important components of cosmic radiation. For a comparison, the boron ions of much lower energy 22...
Article
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The effect of inhibitors of DNA synthesis such as 1-β-D-arabinofuranosyl cytosine (AraC) and hydroxyurea (HU), the antitumor agents used in the clinic, on the formation of double-strand breaks (DSBs) of DNA in human cells during irradiation with protons in the Bragg peak has been studied. It is shown that, in the presence of AraC and HU, the biolog...
Article
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Biological effects of high LET (linear energy transfer) radiation have received increasing attention, particularly in the context of more efficient radiotherapy and space exploration. Efficient cell killing by high LET radiation depends on the physical ability of accelerated particles to generate complex DNA damage, which is largely mediated by LET...
Article
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We report the discovery of frustules of pennate diatoms embedded in a freshly fractured interior surface of the Orgueil CI1 carbonaceous meteorite. Images and element composition by Energy Dispersive X-ray Spectroscopy were obtained with the TESCAN VEGA 3 Scanning Electron Microscope (SEM) in the Astrobiology Sector of the Laboratory of Radiation B...
Article
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DNA double stranded breaks (DSBs) are the most serious type of lesions introduced into chromatin by ionizing radiation. During DSB repair, cells recruit different proteins to the damaged sites in a manner dependent on local chromatin structure, DSB location in the nucleus, and the repair pathway entered. 53BP1 is one of the important players partic...
Article
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The kinetics of formation and elimination of γH2AX/53ВР1 foci induced by accelerated ¹¹B, ²⁰Ne, and ¹⁵N ions with different physical characteristics in human skin fibroblasts have been investigated. The results of the elimination kinetics for radiation-induced γH2AX/53BP1 foci (RIF) reveal that a decrease in the energy and an increase of the linear...
Article
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With the use of the DNA comet assay and immunocytochemical staining, regularities have been studied in the induction and repair of DNA double-strand breaks(DSBs) in human cells after exposure to ⁶⁰Co γ-rays and accelerated heavy ions with different linear energy transfer (LET) in the presence of the DNA repair inhibitors cytosine arabinoside and hy...
Article
The irradiation of brain structures with accelerated heavy charged particles during interplanetary flights or brain tumor therapy raises a number of questions regarding possible neurophysiological disorders in the central nervous system (CNS). Hardly repairable clustered DNA double-strand breaks apparently can have significant influence on the spec...
Article
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Quantification and structural studies of DNA double strand breaks (DSBs) are an essential part of radiobiology because DSBs represent the most serious damage introduced to the DNA molecule by ionizing radiation. Although standard immunofluorescence confocal microscopy has demonstrated its usefulness in a large number of research studies, it lacks t...
Article
The internal structure of an olivine‐rich fragment of the Seymchan meteorite was studied using neutron tomography. The differences in the neutron attenuation coefficients of constituent elements of the studied pallasite and the application of modern mathematical algorithms for a three‐dimensional (3‐D) imaging data analysis allow us to obtain a spa...
Article
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Effects of neuropeptide Semax on behavior reactions and skeletal muscles strength were studied in mice irradiated by protons. Experiments were performed with outbred ICR (CD-I) females, SPF-category. The animals were exposed in the Bragg peak to protons with initial energy 170 MeV and thickness of additional moderator =190 mm. The entry dose was eq...
Article
Chromosome aberrations in human lymphocytes exposed to different doses of particle radiation: 150 MeV and spread out Bragg peak proton beams, 22 MeV/u boron beam and 199 V/u carbon beam were studied. For comparison, an experiment with ⁶⁰Co γ-rays was also performed. We investigated distributions of aberration frequency and the shape of dose–respons...
Article
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Purpose: Our study aimed at evaluating: 1) whether well established variability in radioadaptive response (AR) in various donor blood lymphocytes might be attributed to inter-individual differences in radiosensitivity to different low dose levels; 2) whether AR is reproducibly present over time in the lymphocytes of AR-positive individuals. Experim...
Article
Fundamental research on the harmful effects of ionizing radiation on living cells continues to be of great interest. Recently, priority has been given to the study of high-charge and high-energy (HZE) ions that comprise a substantial part of the galactic cosmic ray (GCR) spectra that would be encountered during long-term space flights. Moreover, pr...
Article
We report that 3',5' cyclic CMP undergoes non-enzymatic di- and trimerization at 20°C under dry conditions upon proton- or UV irradiation. The reaction involves stacking of the cyclic monomers and subsequent polymerization by serial transphosphorylations between the stacked monomers. Proton- and UV-induced oligomerization of 3',5' cyclic CMP demons...
Article
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Model experiments using monkeys (rhesus macaque monkeys) were performed to investigate the neurobiological effects of two components of galactic cosmic radiation, namely, high-energy protons and carbon (¹²C) ions. It has been demonstrated that the irradiation of a monkey’s head with protons at a dose of 3 Gy did not cause any significant changes in...
Article
Model experiments using monkeys (rhesus macaque monkeys) were performed to investigate the neurobiological effects of two components of galactic cosmic radiation, namely, high-energy protons and carbon (12C) ions. It has been demonstrated that the irradiation of a monkey’s head with protons at a dose of 3 Gy did not cause any significant changes in...
Article
The concept of radiation risk to human interplanetary flights is proposed and substantiated. As opposed to traditional approaches, which associate radiation risk to crews mostly with late development of tumor processes in the organism, the notion successful mission outcome probability is introduced. The probability of radiation damage under these c...
Article
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Investigations into the effects of heavy charged particles (HCPs) of galactic space radiation on the central nervous system (CNS) of cosmonauts are crucial for evaluation of radiation risks in exploration class missions. Cytological studies of rat's cerebellum irradiated with carbon 12C heavy ions with the energy of 500 MeV/nucleon were performed a...
Article
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A model of the neutron component of the spacecraft internal radiation fields in the course of a powerful solar proton event was proposed. Field with a small input to the effective dose from protons and photons is generated in the beam of 660 MeV protons on the phasotron at the Joint Institute of Nuclear Researches in Dubna (Russia). The model can b...
Article
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On the phasotron at the Joint Institute of Nuclear Research in Dubna outbred female mice ICR(CD-l) with body mass 26-34 g were irradiated 20, 40 and 60 minutes in the field of secondary neutron radiation by 10 to 30 fractions with mean duration of about 2 min each. Mice were exposed once to 0.5 Gy of y-quanta 60Co and fractionally to 1.0 (0.5 + 0.5...
Article
Full-text available
Formamide (NH2CHO) has been irradiated in condensed phase at 273 K by 11B-boron beams in the presence of powdered meteorites of the chondrite and stony-iron types. Relative to the controls (no radiation or no catalysis), a variegate panel of compounds was observed, including purine and pyrimidine nucleobases (uracil, cytosine, adenine, and guanine)...
Article
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Planning of the deep-space exploration missions raises a number of questions on the radiation protection of astronauts. One of the medical concerns is associated with exposure of a crew to highly energetic particles of galactic cosmic rays. Among many other health disorders, irradiation with these particles has a substantial impact on the central n...
Article
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We studied the effect of carbon ions (12C) with an energy of 500 MeV/nucleon at a dose of 1 Gy on monoamine metabolism in the brains of rats of different ages. Neurochemical parameters that characterize the distribution of noradrenaline (NA), dopamine (DA), serotonin (5-HT), and its metabolites were evaluated during 2 months on days 30 and 90 after...
Article
The modulatory effects of lipid A (Diphosphoryl Lipid A (DLA) and Monophosphoryl Lipid A (MLA)) on apoptosis induction and DNA structure damage (single and double-strand breaks (SSBs and DSBs, respectively)) in peripheral human blood lymphocytes are studied for 60Co gamma-irradiation. It is shown that in the presence of these agents the amount of...
Article
The purpose of the study is to investigate local topological defects in the erythrocyte membranes resulting from the ultraviolet (UV) radiation of blood in vitro. Biological effects in the erythrocytes after exposure to UV radiation at a wavelength of 254 nm are equivalent to those after γ radiation. It has been shown that oxidative processes devel...
Chapter
An extended mathematical model of the UV-induced mutation process in E. coli bacterial cells has been developed. It describes the whole sequence of molecular events involved in nucleotide excision repair of initial damage, replication kinetics and postreplication repair. The model provides ab initio calculation of the number of mismatches as a resu...
Article
Full-text available
A mathematical model of the UV-induced mutation process in Escherichia coli cells with defects in the uvrA and polA genes has been developed. The model describes in detail the reaction kinetics for the excision repair system. The number of mismatches as a result of translesion synthesis is calculated for both wild-type and mutant cells. The effect...
Article
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Outbred CD-1 mice females aged 4 to 4.5-months were investigated in 21-22 hours following total γ-irradiation at 10, 25, 50, 75, 100 and 200 mGy. Loss in bone marrow karyocytes, as well as spleen and thymus mass reductions were significant in the group of animals irradiated at 50 and 200 mGy and less dramatic in mice irradiated at 75 mGy. The orien...
Article
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We investigated the levels of monoamines and their metabolites in certain brain structures of rats at 30 and 90 days after exposure to carbon ions (12C) with an energy of 500 MeV/nucleon. The linear energy transfer and radiation dose were 10.6 keV/µm and 1 Gy, respectively. The concentrations of substances were measured in five structures of the br...
Article
Full-text available
Liquid formamide has been irradiated by high-energy proton beams in the presence of powdered meteorites, and the products of the catalyzed resulting syntheses were analyzed by mass spectrometry. Relative to the controls (no radiation, or no formamide, or no catalyst), an extremely rich, variegate, and prebiotically relevant panel of compounds was o...
Article
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We studied the effects of irradiation with protons at doses of 1 and 2 Gy in the Bragg Peak with an energy of 170 MeV on learning, recall of skills, and concentration of monoamines and their metabolites in the prefrontal cortex, nucleus accumbens, hypothalamus, striatum, and hippocampus of rats. In the T-maze, we performed discriminant learning wit...
Article
We investigated the levels of monoamines and their metabolites in certain brain structures of rats at 30 and 90 days after exposure to carbon ions (12C) with an energy of 500 MeV/nucleon. The linear energy transfer and radiation dose were 10.6 keV/µm and 1 Gy, respectively. The concentrations of substances were measured in five structures of the br...