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Kotamballi N Chidambara Murthy

Kotamballi N Chidambara Murthy
Neuberg Anand Academy of Laboratory Medicine-NAALM

M.Pharma, PhD, Dip R&D. MAMS

About

97
Publications
41,154
Reads
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5,552
Citations
Additional affiliations
September 2012 - May 2014
Gokula Education Foundation (Medical)
Position
  • Head, Research Complementary and Alternate Medicine
October 2006 - June 2011
Vegetable and Fruit Improvement Centre
Position
  • PostDoc Position
Description
  • Worked mainly on establishing cell biology and molecular lab for screening health promoting activity of Fruits and vegetables. Studies over 50 molecules and extracts for their cancer prevention, radical scavenging and other activities.
January 2001 - September 2006
Central Food Technological Research Institute
Position
  • Sr.Research Fellow

Publications

Publications (97)
Article
Full-text available
Introduction Although wound refers to simple cut in the skin, most wounds don't heal because of the various local and systemic factors that lead to its complexity and chronicity. Thus, prior understanding of the status of the wound is necessary and methods that can differentiate between the healing and non-healing wounds at a much earlier stage is...
Article
Full-text available
Although the word wound sounds like a simple injury to tissue, individual's health status and other inherent factors may make it very complicated. Hence, wound healing has gained major attention in the healthcare. The biology wound healing is precise and highly programmed, through phases of hemostasis, inflammation, proliferation and remodeling. Cu...
Article
Full-text available
Characterizing the diverse melon cultivars for nutrition aids in crop improvement and promoting a healthy diet. Here, we used in vitro assays to characterize the nutritional qualities and health-beneficial effects of 30 melon (Cucumis melo L.) genotypes, including 10 improved cultivars, 16 landraces, and 4 wild types collected from different parts...
Article
Full-text available
Non-digestible oligosaccharides (NDOs) from dietary sources have the potential as prebiotics for neuroprotection. Globally, diverse populations suffering from one or the other forms of neurodegenerative disorders are on the rise, and NDOs have the potential as supportive complementary therapeutic options against these oxidative-linked disorders. El...
Article
Full-text available
Fibroblasts and myofibroblasts play a myriad of important roles in human tissue function, especially in wound repair and healing. Among all cells, fibroblasts are group of cells that decide the status of wound as they maintain tissue homeostasis. Currently, the increase in the deleterious effects of chronic wound and their morbidity rate has necess...
Article
In our recent study, we have demonstrated certain limonoids isolated from citrus seeds have induced apoptosis in human pancreatic (Panc-28) cells. In the current study, limonin, nomilin and limonexic acid (LNA) were investigated for possible mode of cytotoxicity in cultured pancreatic cancer (Panc-28) cells. All three limonoids inhibited Panc-28 ce...
Chapter
Melons of the Cucurbitaceous family are known to mankind from time immemorial and are crops of both economic and nutritional importance. Melons can be broadly classified as those which can be consumed as fresh fruits (chandalak, momordica, indicus, ameri, cantalupensis, reticulates, inodorus, casaba, ibericus, makuwa and chinensis), used as vegetab...
Chapter
Grapefruit is a natural cross between pomelo and orange and its origin dates back to the 18th century. It is known for large fruit size, thick peel, and typical citrus fruit taste with bitter tinges, which is due to the high content of naringin, limonoids, and furanocoumarins. Fruit flesh color ranges from white, yellow, orange, to red, which is du...
Article
Fruits and vegetables have long been studied for their potential roles in reducing certain cancer risks. Herein, lyophilized BetaSweet carrots were extracted with hexane, methanol, and water and their chemical constituents were identified by UPLC-QTOF-HR-MS. Methanol extracts exhibited significantly higher radical scavenging activity compared with...
Article
The current protocol of cancer management includes surgery, radiotherapy and chemotherapy. However, these modalities have significant adverse effects and affect the quality of life. Further intensification of treatment is hindered as maximal toxicity levels are reached impeding improvement. Hence researchers are in the quest for adjunctive naturall...
Chapter
Full-text available
Curcumin, (1E,6E)-1,7-Bis (4-hydroxy-3-methoxyphenyl) hepta-1,6-diene-3,5-dione, has potential anti-cancer and other health-promoting activities. Despite its promising pharmacological properties, therapeutic applications of curcumin have been impeded by its limited bioavailability due to its low aqueous solubility at acidic and physiological pH, lo...
Article
Background: Naturally available extracts have gained importance as adjunctive pharmacologic modalities. The earthworm coelomic fluid (ECF) has shown promising in vitro cytotoxic effect in hepatocellular, colorectal, and breast cancers. Few in vitro studies have been performed pertaining to oral cancer. Objective: The objective of this study was to...
Article
Full-text available
Objective: The primary goal of this study was to convert a natural catechin-rich extract into nanoparticles by using a biodegradable and non-toxic polymer Eudragit L 100 to address the various biopharmaceutical problems of catechin. Methods: Nanoparticles were prepared by emulsion solvent evaporation technique using Eudragit L 100 in increasing con...
Article
Full-text available
Objective: The primary goal of this study was to convert a natural catechin-rich extract into nanoparticles by using a biodegradable and non-toxic polymer Eudragit L 100 to address the various biopharmaceutical problems of catechin.Methods: Nanoparticles were prepared by emulsion solvent evaporation technique using Eudragit L 100 in increasing conc...
Preprint
Full-text available
Objective: The aim of the study was to develop sustained release nanocapsules of catechin rich extract (CRE) using Eudragit L 100 as a polymer by emulsion solvent evaporation technique with a major focus on enhancing its bioavailability. Methods: CRE and the polymer were subjected for physical compatibility studies using Fourier transform infrared...
Article
Full-text available
Objective: The aim of the study was to develop sustained release nanocapsules of catechin rich extract (CRE) using Eudragit L 100 as a polymer by emulsion solvent evaporation technique with a major focus on enhancing its bioavailability. Methods: CRE and the polymer were subjected for physical compatibility studies using Fourier transform infrared...
Article
In recent times, naturally occurring substances such as earthworm extracts have been used successfully as an antimicrobial and anti-inflammatory agent in wound healing. It has also shown promising antitumor activity in cervical and gastric cancer. The aim of this systematic review is to analyze the anticancer potentials of earthworm extracts. Sever...
Article
Introduction: The earthworm coelomic fluid (ECF) has shown proven antiproliferative effect against breast, liver, gastrointestinal, and brain cancer, but it is least explored in oral cancer. The present in vitro study is an attempt to investigate the antiproliferative activity of ECF on oral cancer cell line squamous cell carcinoma (SCC)-9. Mater...
Article
Full-text available
Catechin is a phytochemical largely present in Acacia catechu which is known for its biological potential, but its utilization is greatly limited by its low bioavailability. In the present research work catechin rich extract has been chosen for converting it into nanoformulation by emulsion solvent evaporation technique with minimized dosing employ...
Article
Catechin is a phytochemical largely present in Acacia catechu which is known for its biological potential, but its utilization is greatly limited by its low bioavailability. In the present research work catechin rich extract has been chosen for converting it into nanoformulation by emulsion solvent evaporation technique with minimized dosing employ...
Article
To improve bioavailability and enhance colon cancer prevention ability of curcumin, whey protein was used to nanoencapsulate at three different ratios such as 70:30, 50:50 and 35:65 for the first time. The drug loading, entrapment efficiency and structural changes of curcumin was confirmed by quantitative NMR spectroscopy. The nanoparticles prepare...
Article
Obacunone and obacunone glucoside (OG) are naturally occurring triterpenoids commonly found in citrus and other plants of the Rutaceae family. The current study reports the mechanism of cytotoxicity of citrus-derived obacunone and OG on human androgen-dependent prostate cancer LNCaP cells. Both limonoids exhibited time- and dose-dependent inhibitio...
Article
Conventional wisdom suggests that consumption of plant-based foods including, fruits, vegetables, whole grains, cereals, nuts, marine foods (rich in longchain ω-3 fatty acids) are key factors in maintaining healthy lifestyles and helping to combat the risk of chronic disease. Consequently there has been increased interest in research, development a...
Article
The focus of higher education has witnessed a paradigm shift from mere addressing the needs of literacy to cater to wider global challenges. With success of opportunity based learning, it is clear that higher education should prepare an individual with outcome based learning, focusing on cognitive, affective and psychomotor skills. In this regard,...
Article
Objective: The aim of the study was to develop sustained release nanocapsules of catechin rich extract (CRE) using Eudragit L 100 as a polymer by emulsion solvent evaporation technique with a major focus on enhancing its bioavailability. Methods: CRE and the polymer were subjected for physical compatibility studies using Fourier transform infrared...
Article
Full-text available
Catechin is known for its diverse health benefits and poor bioavailability. In the present work catechin rich extract (CRE) has been chosen to develop sustained released nanoparticlesby solvent evaporation technique for enhancing bioavailability. Eudragit L 100 is used as a polymer for formulating CRE into nanoparticles. CRE and polymer were subjec...
Preprint
Full-text available
Catechin is known for its diverse health benefits and poor bioavailability. In the present work catechin rich extract (CRE) has been chosen to develop sustained released nanoparticlesby solvent evaporation technique for enhancing bioavailability. Eudragit L 100 is used as a polymer for formulating CRE into nanoparticles. CRE and polymer were subjec...
Preprint
Full-text available
The aim of the study was to develop and characterize nanoparticles of catechin rich extract (CRE) by solvent evaporation technique. Catechin is a phytochemical having multitude health benefits such as antioxidant, antimicrobial, anti-proliferative, anti-inflammation and other disease preventive properties. In spite of its excellent biological poten...
Article
In the current study, we examined the ability of limonoids, including limonin, limonin glucoside (LG) and curcumin, to inhibit proliferation of human colon cancer (SW480) cells. Additionally, we studied the effect of combining these two classes of natural compounds on inhibition of proliferation and the possible mode of cytotoxicity. The SW480 cell...
Article
Full-text available
Volatile oil from Palestine sweet lime (PSL) was analyzed for their chemical constituents. A total of 24 compounds were identified in hydro-distilled oil by GC–MS with electron impact mode using Zebron (ZB)-5 and ZB wax columns. The PSL volatile oil included 13 monoterpenes, 9 sesquiterpenes, one acyclic ester and one straight chain hydrocarbon. Fo...
Article
For the first time, three coumarins were isolated from the hexane extract of limes (Citrus aurantifolia) and purified by flash chromatography. The structures were identified by NMR (1D, 2D) and mass spectral analyses as 5-geranyloxy-7-methoxycoumarin, limettin, and isopimpinellin. These compounds inhibited human colon cancer (SW-480) cell prolifera...
Article
A review. Traditionally plants were used as medicine and food for providing key health benefits, including prevention of a no. of infectious, chronic, and acute diseases. One of the advantages of plant-derived compds. is their higher margin of safety and their multitude of benefits in prevention of chronic disease and assocd. symptoms; this activit...
Chapter
Although pigments of biological origin are intensely researched for their market potentials and health benefits, their stability, from the maturity status of the source material to their processing and products into which the pigments are incorporated and the storage of those products, is shrouded with many problems. Both extrinsic and intrinsic fa...
Chapter
Diabetes mellitus is a group of metabolic disorders characterized by elevated sugar levels in blood, caused by defects in insulin secretion and further signaling cascades. Among the approaches to overcome diabetes, diet plays a key role in the maintenance of blood sugar level, in the hyper-glycosylation of biomolecules associated with diverse metab...
Article
To identify the chemical constituents of volatile oil from blood orange (Citrus sinensis (L) Osbeck) and understand the possible mechanisms of inhibition of colon cancer cell proliferation. Volatile oil was obtained from blood oranges by hydro-distillation. Nineteen compounds were identified by GC-MS and d-limonene was found to be the major compone...
Article
Texas A&M University, College Station, TX 77845–2119. Poncirus trifoliata (syn. Citrus trifoliata), is a member of Rutaceae family. Fruits of Poncirus trifoliata (trifoliata orange) are well known for traditional medicines in Asia especially for treating allergic diseases, inflammation, ulcers, anti HIV-1 and hepatoxicity. In the present study, lyo...
Article
Fresh Nagami kumquats (Fortunella margarita) were subjected to hydrodistillation using a Clevenger-type apparatus to obtain volatile oil. The chemical composition of the volatile oil was analyzed by GC-MS using Rtx-5 Sil MS and DB Wax columns. A total of 25 volatile compounds were identified by mass spectra, retention index, and comparison with kno...
Article
In the current paper, berberine, an isoquinoline alkaloid, was tested for its chemopreventive potential in colon cancer (SW480) cells. Berberine inhibited proliferation of colon cancer cells in a dose- and time-dependent manner. Interestingly, this compound exhibited minimum toxicity in normal cells at 200 μM. Berberine arrested SW480 cell cycle at...
Article
A number of studies in the recent years have evaluated the anti-proliferative activity of flavonoids. Although certain studies investigated the structure–activity based on the phenotypic assays, no study has correlated the flavonoids structure with the ability to alter gene/protein expression. Present study was focused to understand the structure–f...
Article
Dietary monoterpenes are non-nutritive volatile principles found in citrus and other regularly consumed plant constituents. Monoterpenes are gaining significant importance as major ingredients of functional food and constituents of prophylactic formulations due to their ability to stabilize the symptoms of chronic diseases including cancer. Among t...
Article
Lyophilised nagami kumquat (Fortunella margarita) powder was extracted with five different solvents. Dried extracts of EtOAc and MeOH–water (4:1, v/v) had the highest and lowest total phenolics, respectively, by Folin–Ciocalteu method. LC–MS analysis confirmed the presence of different levels of rutin, narirutin, poncirin, apigenin 8-C-rutinoside a...
Article
Full-text available
The lower incidences of many chronic diseases such as cancer and cardiovascular related ailments are associated with consumption of vegetables rich dietary regimes and this has been substantiated through numerous in vitro, pre-clinical and clinical investigations. Members of cruciferous family are cultivated and widely consumed universally as a par...
Article
A rapid and versatile HPLC-UV method for the separation and quantification of l-citrulline from watermelon was developed for the first time. Freeze dried juice and rind of watermelon were extracted with acidic methanol and analysed by HPLC using 0.03mM phosphoric acid as a mobile phase. The separation was performed using Zorbax SB-Aq, Synergi Hydro...
Article
The study was aimed to purify obacunone and obacunone glucoside (OG) from seeds of Marsh White grapefruit and understand the mode of cytotoxicity of limonoids on colon cancer (SW480) cells. Both limonoids were purified using chromatographic techniques. The structures and purity of limonoids were confirmed by NMR and HPLC analysis, respectively. Oba...
Article
Obacunone is one of the oxygenated triterpenoids found in rutaceae family. It has been demonstrated for various biological activities including inhibition of cancer cells proliferation and tumor. The current study is an attempt to understand the mode of cytotoxicity of obacunone using cultured pancreatic cancer (MDA Panc-28) cells. Obacunone exhibi...
Article
The current study was an attempt to elucidate the mechanism of human colon cancer cell proliferation inhibition by limonin and limonin glucoside (LG) isolated from seeds of Citrus reticulata. The structures of purified compounds were confirmed by NMR and quantified using HPLC. These compounds of more than 95% purity were subjected to proliferation...
Article
In the current study, we have isolated and purified five putative compounds from lime (Citrus aurantifolia Swingle) seeds using Soxhlet extraction and chromatographic separation. The purity and structures of the isolated compounds were identified by TLC, HPLC and mass spectra as limonin, limonexic acid (LNA), isolimonexic acid (ILNA), β-sitosterol...
Article
Lime (Citrus aurantifolia Swingle) is one of the major citrus fruits and widely consumed, but there is limited evidence about its health-promoting properties. Hence, an investigation was conducted to understand the chemopreventive effects of lime juice on pancreatic cancer cells and the possible mechanism for induction of apoptosis using Panc-28 ce...
Article
Mom's conventional wisdom of eating fruits and vegetables to lead a healthy life has evolved with scientific, fact-finding research during the past four decades due to advances in science of "Foods for Health". Epidemiological and prospective studies have demonstrated the vital role of fruits, vegetables, and nuts in reducing the risk of cancer and...
Article
Phytochemicals present in citrus fruits, such as triterpenoids and related bitter compounds were of great concern to citrus industries during last decade due to their unpleasant taste. These same compounds are gaining importance during recent years, due to their proven benefits in prevention of cancer and other chronic diseases. Both limonin and it...
Article
Fruits of Citrus aurantifolia were subjected to hydro-distillation using Clevenger type apparatus to obtain volatile oil. Chemical composition of volatile oil was analysed by GC–MS. Twenty-two compounds representing more than 89.5% of the volatile oil were identified. d-limonene (30.13%) and d-dihydrocarvone (30.47%) were found to be the major comp...