Konstantinos Poulas

Konstantinos Poulas
University of Patras | UP · Department of Pharmacy

Associate Professor

About

198
Publications
54,015
Reads
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4,076
Citations
Citations since 2017
103 Research Items
3049 Citations
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20172018201920202021202220230200400600800
20172018201920202021202220230200400600800
Additional affiliations
January 2003 - present
University of Patras
January 1999 - December 2010
Institut Pasteur international
May 1994 - December 2000
Institut Pasteur International Network
Position
  • PhD Student

Publications

Publications (198)
Article
Full-text available
Background: Although great progress has been made over the past 2 years in the scientific understanding of the biology, epidemiology, and pathogenesis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), case morbidity and fatality rates remain a great concern and continue to challenge the healthcare resources worldwide as novel varian...
Poster
Full-text available
Effect of grape stems extract on behavior and biochemical markers in adult male mice. Korina Atsopardi1,2,3, Dimitrios E. Providas1, Panagiota Giannakopoulou3, Alexandros Voutsinas-Kandilioros1, Christina Dimitriou3, Demetrios Kouretas4, Marigoula Margarity2, Konstantinos Poulas1 1 Laboratory of Molecular Biology and Immunology, Department of Phar...
Poster
Full-text available
Nicotine effects on anxiety behavior and brain biochemical markers in adult male mice and in rat hippocampal slices: patch clamp recondings Authors: Ioannis Plastourgos1,2, Korina Atsopardi1,3, Giuseppe Talani4, Luca Trincas2, Andrea Mastio2, Elisabetta Batzu2 , Konstantinos Poulas3, Enrico Sanna2*, Marigoula Margarity1 1Laboratory of Human and...
Article
The aim of the study was to investigate the impact of the COVID‐19 lockdown on children's and adolescents' mental health in Greece during the lockdown of spring 2020. A cross‐sectional survey of 1232 Greek parents was conducted in spring 2020. 35.1% of parents reported that their children's psychological health was significantly affected. Parental...
Article
Cigarette smoking is associated with impairment of repair mechanisms necessary for vascular endothelium homeostasis. Reducing the exposure to smoke toxicants may result in the mitigation of the harmful effect on the endothelium and cardiovascular disease development. Previous investigations evaluated in vitro the effect of electronic cigarette (EC)...
Article
Electronic nicotine delivery systems (ENDS) can reduce the health risks associated with chronic smoke exposure, and their potential benefits are the subject of intense scientific debate. The international "Replica Study Group" replicated independently three relevant studies from the tobacco industry on the cytotoxic and inflammatory effects of ciga...
Preprint
Full-text available
Inter-laboratory comparison is widely used to ensure quality control among laboratories. In in vitro toxicology studies for tobacco harm reduction (THR), exposure system performance and laboratory proficiency along with product smoke and aerosol stream are tested for variability to assess accuracy. Here we aim to test a novel inter-laboratory setup...
Preprint
Full-text available
Cigarette smoking is associated with impairment of repair mechanisms necessary for vascular endothelium homeostasis. Reducing the exposure to smoke toxicants may result in the mitigation of the harmful effect on the endothelium and cardiovascular disease development. Previous investigations performed by the tobacco industries evaluated in vitro the...
Article
Full-text available
Electronic nicotine delivery systems (ENDS) may reduce health risks associated with chronic exposure to smoke and their potential benefits have been the matter of intense scientific debate. We aimed to replicate three published studies on cytotoxic and inflammatory effects of cigarette smoke and ENDS aerosol in an independent multi-center ring stud...
Article
Full-text available
Soon after the beginning of the severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) pandemic in December, 2019, numerous research teams, assisted by vast capital investments, achieved vaccine development in a fraction of time. However, almost 8 months following the initiation of the European vaccination programme, the need for prospective...
Article
Background Myasthenia gravis (MG) is a rare autoimmune disorder affecting the neuromuscular junction (NMJ). Here, we investigate the genetic architecture of MG via a genome-wide association study (GWAS) of the largest MG data set analysed to date. Methods We performed GWAS meta-analysis integrating three different data sets (total of 1401 cases an...
Article
Importance: SARS coronavirus 2 (SARS­­­-CoV-2) is spread mainly through airborne transmission and colonizes the human upper respiratory tract. It causes coronavirus disease 2019 (COVID-19), which has major therapeutic challenges as there are no treatments to prevent the infection from spreading or the development of the disease in a severe form. Ob...
Article
A featured article in the BMJ, authored by journalists from the Investigative Desk, criticize research about COVID-19 and the cholinergic system by confusing it with tobacco harm reduction and non-pharmaceutical nicotine products. Their criticism is not based on an assessment of the studies content, analysis and presentation, but on allegations abo...
Article
A featured article in the BMJ, authored by journalists from the Investigative Desk, criticize research about COVID-19 and the cholinergic system by confusing it with tobacco harm reduction and non-pharmaceutical nicotine products. Their criticism is not based on an assessment of the studies content but on allegations about conflicts. I present the...
Article
A featured article in the BMJ, authored by journalists from the Investigative Desk, criticize research about COVID-19 and the cholinergic system by confusing it with tobacco harm reduction and non-pharmaceutical nicotine products. Their criticism is not based on an assessment of the studies content but on allegations about conflicts. I present the...
Preprint
Cigarette smoking is the leading cause of preventable deaths worldwide. Electronic Nicotine Delivery Systems (ENDS) may reduce health risks associated with chronic exposure to smoke and their potential benefits have been the matter of intense scientific debate. Here we replicated three key published studies from the Tobacco Industry on cytotoxic an...
Article
Full-text available
Gnomoniopsis castaneae is the cause of the chestnut brown rot but has been also regarded as an important mortality factor for the chestnut gall wasp Dryocosmus kuriphilus. The question to whether G. castaneae could serve as a natural biocontrol agent against insect pests is investigated in the present study. We used three serious insect pests as ex...
Preprint
Full-text available
Efficacious and safe vaccines are needed to contain the COVID-19 pandemic. mRNA and viral vector vaccines are authorized for use in many countries. Cases of thrombosis and/or thrombocytopenia are some of the serious adverse events reported so far. Here we discuss some of these events and try to identify the causative factor of these phenomena.
Article
Full-text available
The COVID-19 pandemic has had an unprecedented and devastating impact on public health, society and economics around the world. As a result, the development of vaccines to protect individuals from symptomatic COVID-19 infections has represented the only feasible health tool to combat the spread of the disease. However, at the same time the developm...
Preprint
Full-text available
Cannabidiol (CBD) is a major active component of the Cannabis plant (Cannabis Sativa L.), which unlike tetrahydrocannabinol (THC) is devoid of euphoria-inducing properties. Broadly, CBD has demonstrated anxiolytic-like, anti-inflammatory and immunomodulatory effects. Concanavalin A (ConA) is a lectin found in the jack bean (Canavalia ensiformis) an...
Article
The innate immune cells play an important role in handling early infections, and can eliminate them completely up to a certain threshold. Beyond that threshold they take up their role in “The Resolution of Inflammation”. The recognition of the SARS-CoV-2 antigen triggers an eicosanoid storm and initiates a robust inflammatory response. This establi...
Article
The innate immune cells play an important role in handling early infections, and can eliminate them completely up to a certain threshold. Beyond that threshold they take up their role in “The Resolution of Inflammation”. The recognition of the SARS-CoV-2 antigen triggers an eicosanoid storm and initiates a robust inflammatory response. This establi...
Article
Full-text available
The human immune system has a generic response strategy to viral infections that begins with local inflammation at the infected site along with the initiation of a primary innate immune response. Beyond a certain threshold, innate immune cells cannot handle the infection, and they call upon adaptive immunity. The innate cells then take up their rol...
Article
Full-text available
The human immune system has a generic response strategy to viral infections that begins with local inflammation at the infected site along with the initiation of a primary innate immune response. Beyond a certain threshold, innate immune cells cannot handle the infection, and they call upon adaptive immunity. The innate cells then take up their rol...
Article
Full-text available
https://www.sciencedirect.com/science/article/pii/S2214750021000469 Abstract Alcohol consumption is associated with multiple diseases and might contribute to vulnerability to SARS-CoV-2 infection. It can also catalyze exacerbations of mental and organic illnesses and predispose to behaviors with an increased risk of infection, severity of disease b...
Article
Full-text available
The outbreak of the coronavirus disease 2019 (COVID‐19) has gathered 1 year of scientific/clinical information. This informational asset should be thoroughly and wisely used in the coming year colliding in a global task force to control this infection. Epidemiology of this infection shows that the available estimates of SARS‐CoV‐2 infection prevale...
Preprint
Full-text available
Objectives The coronavirus disease 2019 (COVID-19) pandemic and mitigation measures based on social distancing are expected to have serious adverse effects on mental health. This cross-sectional study aimed to examine self-reported changes in the mental health status of Greek adults. Study design The current study is a primary research conducted o...
Article
Full-text available
The common grape vine, Vitis vinifera, is a widely known plant with commercial and pharmacological value. The plant hosts a variety of microorganisms known as endophytes that can live within the tissues of the plant for a considerable time period, or even their whole life cycle. The fungus Beauveria bassiana is a well-studied endophyte which can co...
Article
Full-text available
Background: Colposcopy has a key role in the diagnostic work-up and management of abnormal cervical cytology, but it might generate negative feelings of mainly anxiety and pain to the patients undergoing such examination. These feelings are interrelated, with the anxiety fueling the painful sensations. The aim of our study was to investigate the ef...
Article
Full-text available
SARS-CoV-2 is the coronavirus that originated in Wuhan in December 2019 and has spread globally. Studies have shown that smokers are less likely to be diagnosed with or be hospitalized for COVID-19 but, once hospitalized, have higher odds for an adverse outcome. We have previously presented the potential interaction between SARS-CoV-2 Spike glycopr...
Article
Full-text available
Background There is a lot of debate about the effects of smoking on COVID-19. A recent fixed-effects meta-analysis found smoking to be associated with disease severity among hospitalized patients, but other studies report an unusually low prevalence of smoking among hospitalized patients. The purpose of this study was to expand the analysis by calc...
Article
Full-text available
SARS-CoV-2 infection was announced as a pandemic in March 2020. Since then, several scientists have focused on the low prevalence of smokers among hospitalized COVID-19 patients. These findings led to our hypothesis that the Nicotinic Cholinergic System (NCS) plays a crucial role in the manifestation of COVID-19 and its severe symptoms. Molecular m...
Article
Numerous molecular biological experiments performed throughout the world require the detection or quantification of a protein of interest. Western blotting is one of the most popular techniques used for this purpose and offers quantitative information with the aid of specialized software. However, its dependence on the picture that is captured, and...
Article
Numerous molecular biological experiments performed throughout the world require the detection or quantification of a protein of interest. Western blotting is one of the most popular techniques used for this purpose and offers quantitative information with the aid of specialized software. However, its dependence on the picture that is captured, and...
Article
Full-text available
COVID-19 pandemic mitigation strategies are mainly based on social distancing measures and healthcare system reinforcement. However, many countries in Europe and elsewhere implemented strict, horizontal lockdowns because of extensive viral spread in the community which challenges the capacity of the healthcare systems. However, strict lockdowns hav...
Preprint
Full-text available
Background Myasthenia Gravis (MG) is a rare autoimmune disorder affecting the neuromuscular junction. Here, we investigate the genetic architecture of MG performing a genomewide association study (GWAS) of the largest MG dataset analyzed to date. Methods We integrated GWAS from three different datasets (1,401 cases, 3,508 controls) and performed M...
Preprint
Full-text available
Background Mental health effects of the COVID-19 pandemic and the subsequent lockdown measures are expected to be profound. Aim The aim of the present study was to investigate the impact of the pandemic and the lockdown on children’s and adolescents’ mental health in Greece. Methods A cross-sectional survey of 1,232 Greek parents of children and...
Article
Full-text available
The ubiquitin pathway required for most proteins' targeted degradation involves three classes of enzymes: E1-activating enzyme, E2-conjugating enzyme, and E3-ligases. The human Ark2C is the single known E3 ligase that adopts an alternative, Ub-dependent mechanism for the activation of Ub transfer in the pathway. Its RING domain binds both E2-Ub and...
Article
Full-text available
SStored product pests can be detrimental to agricultural produce. As much as chemical pesticides are effective control agents, they involve several environmental and health risks. Within the framework of studies on alternative pest management methods, interest has focused on a plethora of plants whose extracts have demonstrated promising action as...
Article
Full-text available
The present study investigates the interaction between cannabidiol (CBD) oil and three biopesticides: Azatin and two baculovirus formulations (Madex and Helicovex), both separately and in combination, in order to investigate their interaction against adults of four major coleopteran stored-product pests: Sitophilus zeamais (Coleoptera: Curculionida...
Article
Full-text available
Electronic cigarettes are constantly gaining ground as they are considered less harmful than conventional cigarettes, and there is also the perception that they may serve as a potential smoking cessation tool. Although the acute effects of electronic cigarette use have been extensively studied, the long-term potential adverse effects on human healt...
Article
Full-text available
SARS-CoV-2 is the coronavirus that originated in Wuhan in December 2019 and has spread globally. The observation of a low prevalence of smokers among hospitalized COVID-19 patients has led to the development of a hypothesis that nicotine could have protective effects by enhancing the cholinergic anti-inflammatory pathway. Based on clinical data and...
Article
Full-text available
Wound healing constitutes an essential process for all organisms and involves a sequence of three phases. The disruption or elongation of any of these phases can lead to a chronic or non-healing wound. Electrical stimulation accelerates wound healing by mimicking the current that is generated in the skin after any injury. Here, we sought to identif...
Article
Full-text available
While SARS-CoV-2 uses Angiotensin Converting Enzyme 2 (ACE2) as the receptor for cell 17 entry, it is important to examine for other potential interactions between the virus and other cell 18 receptors. Based on the clinical observation of low prevalence of smoking among hospitalized 19 COVID-19 patients, we examined and identified a "toxin-like" a...
Article
Full-text available
Chemical storage pest control is interlinked with many challenges such as environmental pollution and toxicity to humans and animals. Alternative tools are thus being increasingly researched and applied to supplement and/or substitute old-fashioned chemical means. Entomotoxic proteins, such as the lectins of leguminous seeds, have been shown to be...
Article
Full-text available
Background There is insufficient information about risk factors for COVID-19 diagnosis and adverse outcomes from low and middle-income countries (LMICs). Objectives We estimated the association between patients’ characteristics and COVID-19 diagnosis, hospitalisation and adverse outcome in Mexico. Methods This retrospective case series used a pub...
Article
Full-text available
Objective: Globally, human papillomavirus (HPV) is one of the most common sexually transmitted diseases in both men and women. The objective of this study was to investigate the knowledge, attitudes, and behaviour of educators and health professionals regarding the prevention of HPV infection in Greece. The analysis of the results shows that most...
Article
Full-text available
Background: The purpose of this study was to examine the prevalence and effects of current smoking on adverse outcomes among hospitalized COVID-19 patients. Methods: A systematic review of the literature (PubMed) identified 18 (from a total of 1398) relevant studies. Pooled current smoking prevalence was compared with the gender-adjusted and gen...
Preprint
Full-text available
Background: There is insufficient information about risk factors for COVID-19 diagnosis and adverse outcomes from low and middle-income countries (LMICs). Objectives: We estimated the association between patients characteristics and COVID-19 diagnosis, hospitalization and adverse outcome in Mexico. Methods: This retrospective case series used a pub...
Article
According to numerous recent publications, the COVID-19 patients have lymphopenia, higher infection-related biomarkers and several elevated inflammatory cytokines (i.e. tumor necrosis factor (TNF)-α, interleukin IL-2R and IL-6). The total number of B cells, T cells and NK cells are significantly decreased. RNA viruses, SARS-CoV-2 included, hit the...
Preprint
Full-text available
While SARS-CoV-2 uses angiotensin converting enzyme 2 (ACE2) as the receptor for cell entry, it is important to examine for other potential interactions between the virus and other cell receptors. Based on the clinical observation of low smoking prevalence among hospitalized COVID-19 patients, we recently identified a "toxin-like" amino acid (aa) s...
Article
Full-text available
Fungi are eukaryotic microorganisms with many forms and certain demands about the soil they colonize, which reveal the conditions that prevail therein. Although they can often kill pests without damaging plants or humans, they are not widely used as pesticides as they are not easy to handle and require much time before they act. Nevertheless, insec...
Preprint
Full-text available
Smoking is a risk factor for respiratory infections and there is reasonable concern that it may affect COVID-19 susceptibility and severity. Recent studies have focused on the interaction between smoking (and nicotine) and ACE2 expression, suggesting that ACE2 up-regulation could contribute to enhanced viral cell entry. However, case series have sh...
Article
Full-text available
Corona Virus Disease 2019 (COVID-19) is a disease caused by a novel coronavirus, SARS-CoV-2. On 23 March, we presented for the first time evidence of a low prevalence of smoking among hospitalized patients with COVID-19 in China. On 3 April, we were the first to establish the hypothesis that nicotine may be beneficial for COVID-19 patients and shou...
Article
Corona Virus Disease 2019 (COVID-19) is a disease caused by a novel coronavirus, SARS-CoV-2. On 23 March, we presented for the first time evidence of a low prevalence of smoking among hospitalized patients with COVID-19 in China. On 3 April, we were the first to establish the hypothesis that nicotine may be beneficial for COVID-19 patients and shou...
Article
Full-text available
According to numerous recent publications, the COVID-19 patients have lymphopenia, higher infection-related biomarkers and several elevated inflammatory cytokines (i.e. tumor necrosis factor (TNF)-α, interleukin IL-2R and IL-6). The total number of B cells, T cells and NK cells are significantly decreased. RNA viruses, SARS-CoV-2 included, hit the...
Article
Background/aim: The winemaking procedure results in the generation of stems, a by-product that is harmful to the environment. Concomitantly, stems are rich in polyphenols and, hence, they are putatively beneficial for human health. Materials and methods: In this study, the grape stem extracts derived from three native Greek vine varieties, namel...
Article
Full-text available
The number and popularity of electronic nicotine delivery systems (ENDS) and especially e-cigarettes (e-cigs) have been increasing in the last decade. Although ENDS owe their popularity to excluding the harmful chemicals that are present in tobacco smoke, there is a debate whether they are safe, regulated, and as harmless as they are assumed to be...
Poster
Full-text available
Cannabidiol (CBD) is a major active component of the Cannabis plant, which has demonstrated anxiolytic-like, antidepressant-like, neuroprotective and anti-inflammatory properties and unlike tetrahydrocannabinol (THC), is devoid of euphoria-inducing properties. Concanavalin A (ConA) is a lectin found in the jack bean (Canavalia ensiformis) and has b...
Article
Full-text available
A sensitive analytical method was developed and validated for the quantification of cotinine in mouse plasma after smoking exposure of 0.5, 1.0 and 1.5 commercially available cigarettes, using Liquid chromatography tandem mass spectrometry. The method was validated over a linear concentration range of 0.075‐20.0 ng/mL with the R2 value being higher...