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Konstantinos Mitsakakis

Konstantinos Mitsakakis
Hahn-Schickard-Gesellschaft für angewandte Forschung e.V.

PhD

About

47
Publications
4,798
Reads
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719
Citations
Additional affiliations
January 2016 - present
Hahn-Schickard-Gesellschaft für angewandte Forschung eV
Position
  • Business Development Manager
September 2012 - December 2015
Hahn-Schickard-Gesellschaft für angewandte Forschung eV
Position
  • Project Manager
September 2011 - August 2012
University of Freibug
Position
  • PostDoc Position
Description
  • Fellowship provided by the Alexander von Humboldt Foundation
Education
February 2006 - December 2010
Unversity of Crete
Field of study
  • Materials Science & Technology
October 2003 - December 2005
Aristotle University of Thessaloniki
Field of study
  • Interdisciplinary Master Programme on Nanoscience & Nanotechnology
October 1999 - June 2003
University of Crete, Greece
Field of study
  • Physics

Publications

Publications (47)
Article
Full-text available
In this paper, we present the ImmunoDisk, a fully automated sample-to-answer centrifugal microfluidic cartridge, integrating a heterogeneous, wash-free, magnetic- and fluorescent bead-based immunoassay (bound-free phase detection immunoassay/BFPD-IA). The BFPD-IA allows the implementation of a simple fluidic structure, where the assay incubation, b...
Article
Full-text available
Periodontitis and dental caries are two major bacterially induced, non-communicable diseases that cause the deterioration of oral health, with implications in patients' general health. Early, precise diagnosis and personalized monitoring are essential for the efficient prevention and management of these diseases. Here, we present a disk-shaped micr...
Article
Full-text available
This study investigated the potential of salivary bacterial and protein markers for evaluating the disease status in healthy individuals or patients with gingivitis or caries. Saliva samples from caries- and gingivitis-free individuals (n = 18), patients with gingivitis (n = 17), or patients with deep caries lesions (n = 38) were collected and anal...
Article
Full-text available
Oral health is important not only due to the diseases emerging in the oral cavity but also due to the direct relation to systemic health. Thus, early and accurate characterization of the oral health status is of utmost importance. There are several salivary biomarkers as candidates for gingivitis and periodontitis, which are major oral health threa...
Article
Full-text available
Background In this work, a platform was developed and tested to allow to detect a variety of candidate viral, bacterial and parasitic pathogens, for acute fever of unknown origin. The platform is based on a centrifugal microfluidic cartridge, the LabDisk (“FeverDisk” for the specific application), which integrates all necessary reagents for sample-...
Article
Full-text available
We present a simple and fast one-step heterogeneous immunoassay, with performance characteristics that can enable easy and versatile adaptation to miniaturized, automated point-of-care systems. This novel analytical method uses magnetic and fluorescent beads as capture and detection agents respectively. Its main feature is the measurement of the fl...
Article
Periodontitis and dental caries are two major bacterially induced, non-communicable diseases that cause the deterioration of oral health, with implications in patients’ general health. Early, precise diagnosis and personalized monitoring are essential for the efficient prevention and management of these diseases. Here, we present a disk-shaped micr...
Article
Full-text available
Antibiotic resistance poses a global threat, which is being acknowledged at several levels, including research, clinical implementation, regulation, as well as by the World Health Organization. In the field of oral health, however, the issue of antibiotic resistances, as well as of accurate diagnosis, is underrepresented. Oral diseases in general w...
Article
Full-text available
Oral health is maintained by a healthy microbiome, which can be monitored by state-of-the art diagnostics. Therefore, this study evaluated the presence and quantity of ten oral disease-associated taxa (P. gingivalis, T. forsythia, T. denticola, F. nucleatum, C. rectus, P. intermedia, A. actinomycetemcomitans, S. mutans, S. sobrinus, oral associated...
Article
Respiratory Tract Infections (RTIs) are among the top reasons for visiting a General Practitioner (GP) and the main cause of unnecessary antibiotic prescriptions. Reducing inappropriate use is essential to decrease antibiotic resistance and adverse events. The goal of the Eurostars project "Respiotic" is to develop a new point-of-care (POC) platfor...
Article
We present the RespiDisk enabling the fully automated and multiplex point-of-care (POC) detection of (currently) up to 19 respiratory tract infection (RTI) pathogens from a single sample based on reverse transcriptase polymerase chain reaction (RT-PCR). RespiDisk comprises a RTI-specific implementation of the centrifugal microfluidic LabDisk platfo...
Article
Effective mosquito monitoring relies on the accurate identification and characterization of the target population. Since this process requires specialist knowledge and equipment that is not widely available, automated field-deployable systems are highly desirable. We present a centrifugal microfluidic cartridge, the VectorDisk, which integrates Taq...
Article
Full-text available
Saliva offers many advantages for point-of-care (PoC) diagnostic applications due to non-invasive, easy, and cost-effective methods of collection. However, the complex matrix with its non-Newtonian behavior and high viscosity poses handling challenges. Several tedious and long pre-analytic steps, incompatible with PoC use, are required to liquefy a...
Conference Paper
We present three concepts for centrifugal microfluidics reducing ethanol carry-over in magnetic bead-based nucleic acid (NA) extraction. Ethanol carry-over is critical regarding inhibition of downstream NA amplification. We identified two possible carry-over pathways: Liquid co-transport within bead-clusters and vapor diffusion. For the first time,...
Article
Full-text available
The emergence and spread of antimicrobial resistance is one of the major global issues currently threatening the health and wealth of nations, with effective guidelines and intervention strategies urgently required. Such guidelines and interventions should ideally be targeted at individuals, communities, and nations, requiring international coordin...
Article
Most patients with acute infectious diseases develop fever, which is frequently a reason to visit health facilities in resource-limited settings. The symptomatic overlap between febrile diseases impedes their diagnosis on clinical grounds. Therefore, the World Health Organization promotes an integrated management of febrile illness. Along this line...
Article
Full-text available
Antimicrobial susceptibility testing (AST) technologies help to accelerate the initiation of targeted antimicrobial therapy for patients with infections and could potentially extend the lifespan of current narrow-spectrum antimicrobials. Although conceptually new and rapid AST technologies have been described, including new phenotyping methods, dig...
Article
Full-text available
General practitioners stand at the front line of healthcare provision and have a pivotal role in the fight against increasing antibiotic resistance. In this respect, targeted antibiotic prescribing by general practitioners would help reduce the unnecessary use of antibiotics, leading to reduced treatment failures, fewer side-effects for patients an...
Article
Centrifugal microfluidics enables the rapid execution of complex blood sample analyses in a fully automated manner at the point of care. However, during blood sample addition, the cartridge is at rest and centrifugal forces are not present to allocate the sample in a controlled way. We present a versatile approach for the user-friendly, well-contro...
Article
Full-text available
Monitoring malaria prevalence in humans, as well as vector populations, for the presence of Plasmodium, is an integral component of effective malaria control, and eventually, elimination. In the field of human diagnostics, a major challenge is the ability to define, precisely, the causative agent of fever, thereby differentiating among several cand...
Article
In centrifugal microfluidics, dead volumes in valves downstream of mixing chambers can hardly be avoided. These dead volumes are excluded from mixing processes and hence cause a concentration gradient. Here we present a new bubble mixing concept which avoids such dead volumes. The mixing concept employs heating to create a temperature change rate (...
Conference Paper
We report the successful implementation of twelve assays for detecting fever-causing pathogens on a centrifugal microfluidic LabDisk platform (FeverDisk). The panel was selected to diagnose tropical regions’ most prevalent endemic and epidemic fever-causing diseases. The FeverDisk was used by local scientific staff in two clinical settings (Dakar,...
Poster
Centrifugal microfluidics offer the possibility to rapidly analyze blood samples at the point-of-care (POC). However, during blood sample addition the cartridge is at rest and centrifugal forces cannot be used to allocate the sample in a controlled manner. This may represent a risk to the user’s safety, as well as the test result. We present a vers...
Article
We present a fully automated centrifugal microfluidic method for particle based protein immunoassays. Stick-pack technology is employed for pre-storage and release of liquid reagents. Quantitative layout of centrifugo-pneumatic particle handling, including timed valving, switching and pumping is assisted by network simulations. The automation is ex...
Conference Paper
Centrifugal microfluidics enable fully automated multiplex nucleic acid testing. In such applications, all reagents must be pre-stored. We present a solution for a generic problem in centrifugal microfluidics that occurs when a solid reagent must be homogeneously rehydrated prior to an aliquoting step. Because the liquid in the downstream channel d...
Conference Paper
Introduction Point-of-care tests (POCT) enable fast diagnosis of infectious diseases especially in resource-poor settings, where they are typically operated by minimally-trained personnel and replace missing clinical infrastructure [1]. Problems regarding user- and patient safety and the system’s performance can arise during the sample intake due t...
Article
Global healthcare systems are struggling with the enormous burden associated with infectious diseases, as well as the incessant rise of antimicrobial resistance. In order to adequately address these issues, there is an urgent need for rapid and accurate infectious disease diagnostics. The H2020 project DIAGORAS aims at diagnosing oral and respirato...
Article
Full-text available
Malaria is a life-threatening disease that caused more than 400,000 deaths in sub-Saharan Africa in 2015. Mass prevention of the disease is best achieved by vector control which heavily relies on the use of insecticides. Monitoring mosquito vector populations is an integral component of control programs and a prerequisite for effective intervention...
Article
Full-text available
Global healthcare systems are struggling with the enormous burden associated with infectious diseases, as well as the incessant rise of antimicrobial resistance. In order to adequately address these issues, there is an urgent need for rapid and accurate infectious disease diagnostics. The H2020 project DIAGORAS aims at diagnosing oral and respirato...
Conference Paper
We demonstrate for the first time the highly precise and fully automated manufacturing of tubular-shaped composite-foil pouches for the pre-storage of liquid reagents in lab-on-a-chip disposables. Those stick-packs are miniaturized and easy to handle packages and enable: i) the protection of reagents from external influences (e.g. irradiation, vapo...
Conference Paper
The Lab-on-a-Chip Design & Foundry Service offers the rapid development of point-of-care systems, which automate diagnostic assays delivered by the customer. The automation is done on a centrifugal microfluidic lab-on-a-chip platform, the LabDisk. The customer profits from the utilization of unit operations in the development process. Those unit op...
Article
Full-text available
Surface acoustic wave sensors with integrated microfluidics for multi-sample sensing have been implemented in this work towards the quantitative correlation of the acoustic signal with the molecular weight of surface bound proteins investigating different interaction/binding conditions. The results are presented for: (i) four different biotinylated...
Article
There have been considerable advances in the field of nanotechnology-based biosensors and diagnostics (NBBD) during the last two decades. These include the production of nanomaterials (NMs), employing them for new biosensing and diagnostic applications, their extensive characterization for in vitro and in vivo applications, and toxicity analysis. A...
Article
Full-text available
The present work demonstrates for the first time patterning of a ready-to-use biosensor with several different biomolecules using Dip-Pen Nanolithography (DPN) for the development of a procedure towards more rapid and efficient multi-sample detection. The biosensor platform used is based on a Surface Acoustic Wave (SAW) device integrated with a par...
Article
This work describes the application of a newly emerged biosensing configuration incorporating a Surface Acoustic Wave device integrated with a multi-channel microfluidic module for the rapid and efficient analysis of cardiac markers. The examined cardiac markers of creatine kinase MB (CK-MB), cardiac reactive protein (CRP), D-dimer and pregnancy-as...
Article
The current work describes a novel setup for multi-sample biomolecular analysis. It is based on the assembly of a dual acoustic device chip with a four-channel microfluidic module, forming an array of eight available domains for experiments. Initially, multiple detection was demonstrated via the specific interaction of neutravidin with four differe...
Article
We have studied the formation of histone Hv1-DNA complexes using an acoustic biosensor and AFM imaging. Our results show that DNA and histone molecules aggregate into amorphous accumulations which form a compact rigid layer on the sensor's surface. By measuring changes in the acoustic wave amplitude, it was possible to titrate surface bound DNA wit...
Conference Paper
This work describes the design and fabrication of a novel approach to achieve multi-sample sensing based on a single Surface Acoustic Wave (SAW) device. This is achieved with the integration of a multi-channel microfluidic module, which divides a SAW device into N discrete sub-areas, each of them capable of hosting an individual experiment. The fab...
Article
DNA bending plays a significant role in many biological processes, such as gene regulation, DNA replication, and chromosomal packing. Understanding how such processes take place and how they can, in turn, be regulated by artificial agents for individual oriented therapies is of importance to both biology and medicine. In this work, we describe the...
Article
This work focuses on the development of a multi-analyte biosensor, based on a Surface Acoustic Wave (SAW) device. The novelty of the concept lies in the way of achieving multiplicity: instead of the “traditional” way of a sensor element array, multiplicity is induced by compartmentalization of a single sensor, achieved via microfluidics (“microflui...

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Projects

Projects (3)
Archived project
Disc-shaped point-of-care platform for infectious disease diagnosis https://www.youtube.com/watch?v=UvcZwOXTRuk&feature=youtu.be%20
Project
Chair/bedside diagnosis of oral and respiratory tract infections, and identification of antibiotic resistances for personalised monitoring and treatment (www.diagoras.eu)
Project
Automated diagnostic platform, data management system and innovative communication tool, for improving the impact of malaria vector control interventions. http://www.dmc-malvec.eu/