Konstantin Purtov

Konstantin Purtov
Russian Academy of Sciences | RAS · Institute of Biophysics

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28
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708
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Publications

Publications (28)
Article
The marine polychaete Chaetopterus variopedatus (Renier) (family Chaetopteridae) is a cosmopolitan species complex, consisting of distinct populations/ subspecies. The worms release glowing (460 nm) clouds of mucus when disturbed, and their parapodia often glow brightly. Currently, it is still unclear how exactly the bioluminescence system of these...
Article
Full-text available
A key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. The properties of fungal luciferase and their relationship with its structure are interesting both for improving autoluminescent systems already created on its basis and for creating new ones. Therefore, it is extremely important...
Article
The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein i...
Article
This is the first study to obtain a high-purity luciferase from the fungus Neonothopanus nambi biomass that is suitable for subsequent sequencing.
Article
Full-text available
The structure of fungal oxyluciferin was determined, the enzymatic bioluminescence reaction under substrate saturation conditions with discrete monitoring of formed products was conducted, and the structures of the end products of the reaction were established. On the basis of these studies, the scheme of oxyluciferin degradation to the end product...
Article
Full-text available
By determining the components involved in the bioluminescence process in luminous and nonluminous organs of the honey fungus Armillaria mellea, we have established causes of partial luminescence of this fungus. The complete set of enzymes and substrates required for bioluminescence is formed only in the mycelium and only under the conditions of fre...
Article
This study investigates the possibility of using a detonation nanodiamond as a targeted drug-delivery vehicle. Supramolecular complexes composed of nanodiamond particles and address protein molecules (nanodiamond-IgGI125 and RAM-nanodiamond-BSAI125) have been obtained. In vitro experiments have revealed that these complexes are stable and exhibit h...
Patent
SUBSTANCE: invention relates to instrument making. Working medium contains optically transparent dispersion medium in which carbon nanoparticles are dispersed, where the carbon nanoparticles used are detonation synthesis nanodiamonds having high colloidal stability in amount of 1.0-3.0 wt %, and the dispersion medium used is distilled water. The me...
Article
Full-text available
The nonlinear scattering of light under the conditions of optical limiting of nanosecond pulsed laser radiation at a wavelength of 1064 nm in a nanodiamond (ND) hydrosol has been experimentally studied. Superstable hydrosols were obtained from detonation NDs with a modified surface. Using an improved scheme of z scanning, it is shown that a decreas...
Article
Full-text available
Surface of detonation nanodiamonds was functionalized for the covalent attachment of immunoglobulin, and simultaneously bovine serum albumin and Rabbit Anti-Mouse Antibody. The nanodiamond-IgG(I125) and RAM-nanodiamond-BSA(I125) complexes are stable in blood serum and the immobilized proteins retain their biological activity. It was shown that the...
Article
Colloidal stability is one of the critical factors for the use of nanodiamonds as potential enterosorbents. Although nanodiamonds are believed to be a promising enterosorbent, colloidal stability in hydrosols of raw polydispersed nanodiamonds produced by detonation is typically low. Surface modification and fractionation significantly improves coll...
Article
Nanodiamonds synthesized by detonation have been found not to immobilize the ring form of pUC19 plasmid DNA. Linear pUC19 molecules with blunt ends, prepared by restriction of the initial ring form of pUC19 DNA, and linear 0.25–10 kb DNA fragments are adsorbed on nanodiamonds. The amount of adsorbed linear DNA molecules depends on the size of the m...
Article
For this study, we selected the reaction of coupled oxidation of phenols and their derivatives with 4-aminoantipyrine (1-phenyl-2,3-dimethyl-4-aminopyrazolone; 4-AAP) in the presence of hexacyanoferrate (III) [ K 3 Fe(CN) 6 ] at pH 10 ± 0.2 [5], which forms the basis of the photometric method for detecting the environmental pollution with phenols a...
Article
This paper discusses the prospects for using modified detonation nanodiamonds with novel properties as a new promising material for the development of nanotechnologies intended for biological and medical purposes. The authors report that nanodiamonds synthesized by detonation can be used to further purify commercial protein preparations and that en...
Article
The contamination of feedstuff and foodstuff with toxins of microscopic fungi, in particular, aflatoxins, presents a danger to animal and human health [1], and neutralization of the adverse effect of these compounds is a topical problem. One of the ways to solve this problem is application of various sorbents binding mycotoxins and thereby decreasi...
Article
New high-efficiency and rather simple method for protein separation and purification in solution is created, which is based on principles of coordination and ligand-exchange interactions of bivalent metal ions with protein molecules. The method includes protein precipitation via formation of protein-metal chelate complex at adding bivalent metal io...
Article
Using site directed mutagenesis, the conservative residue Cys158 of recombinant apoobelin was substituted for serine (C158S, S-mutant) or alanine (C158A, A-mutant). These point mutations resulted in significant changes in the apoobelin structure accompanied by slowing of photoprotein complex formation, decrease of its stability, and changing of its...
Article
The work reviews examples of complex nanostructures made up of radically different in their chemical nature and physical properties nanoparticles produced by explosive synthesis and protein molecules as "binding" agents.

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