Konstantin Andreev

Konstantin Andreev
St. Jude Children's Research Hospital · Department of Infectious Diseases

Ph.D. in Biology

About

23
Publications
6,581
Reads
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262
Citations
Citations since 2017
16 Research Items
248 Citations
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Introduction
I am passionate about combining molecular biology with physics experimental approaches to address fundamental biological questions. The scope of my research interest is focused on: • membrane biophysics • peptide immunotherapy • drug discovery and targeted delivery • molecular structure of ultrathin films at the air-liquid interface.
Additional affiliations
November 2017 - December 2021
Howard Hughes Medical Institute
Position
  • Postdoctoral Research Associate
November 2017 - December 2021
Northwestern University
Position
  • Postdoctoral Research Fellow
September 2010 - April 2017
Illinois Institute of Technology
Position
  • Research Assistant

Publications

Publications (23)
Cover Page
Full-text available
The cover art depicts the arrangement of surfactant components at the alveolar surface in lungs. Vesicles of pulmonary surfactant in the subphase prevent interfacial monolayers from collapse by expanding the ordered phase. The phospholipid‒cholesterol distribution displays a hexagonal unit cell revealed by X-ray diffraction. Cover design conceived...
Article
When compressed by the shrinking alveolar surface area during exhalation, films of pulmonary surfactant in situ reduce surface tension to levels at which surfactant monolayers collapse from the surface in vitro. Vesicles of pulmonary surfactant added below these monolayers slow collapse. X-ray scattering here determined the structural changes induc...
Preprint
Full-text available
When compressed by the shrinking alveolar surface area during exhalation, films of pulmonary surfactant in situ reduce surface tension to levels, at which surfactant monolayers collapse from the surface in vitro . Vesicles of pulmonary surfactant added below these monolayers slow collapse. X-ray scattering here determined the structural changes tha...
Conference Paper
Full-text available
Pulmonary surfactant is the mixture of lipids and proteins that lowers surface tension in the lungs. The material forms a thin film on the aqueous layer that lines the alveolar air-sacks. When compressed by the shrinking alveolar surface area during exhalation, the surfactant film achieves surface tensions < 5 mN/m. The molecular arrangement of the...
Article
Full-text available
Background Gangliosides are an essential component of eukaryotic plasma membranes implicated in multiple physiological processes. Little is known about molecular mechanisms underlying the distribution and functions of membrane gangliosides. The overwhelmingly complex organization of glycocalyx impedes the structural analysis on cell surface and the...
Article
Full-text available
Gram-negative bacteria are protected from their environment by an outer membrane that is primarily composed of lipopolysaccharides (LPS). Under stress, pathogenic serotypes of Salmonella enterica remodel their LPS through the PhoPQ two-component regulatory system that increases resistance to both conventional antibiotics and antimicrobial peptides...
Article
Full-text available
Synthetic polymers mimicking antimicrobial peptides have drawn considerable interest as potential therapeutics. N‐substituted glycines, or peptoids, are recognized by their in vivo stability and ease of synthesis. Peptoids are thought to act primarily on the negatively charged lipids that are abundant in bacterial cell membranes. A mechanistic unde...
Conference Paper
Full-text available
Cancer is one of the most serious threats to global public health. Aside from the surgical treatment, radiation and immunotherapy, chemotherapeutic approaches remain at the frontline of curative and palliative care for oncology diseases. Due to the current limitations of conventional chemotherapies, identifying potential targets for novel anticance...
Conference Paper
Full-text available
The outermost surface of Gram-negative bacteria is almost entirely composed of lipopolysaccharides. These lipids are targeted by host defense peptides as an innate immune response. Pathogenic serotypes of Salmonella enterica possess the two-component regulatory system, PhoPQ, that modifies lipopolysaccharides in response to the presence of host def...
Conference Paper
Full-text available
Hydrophobic interactions govern specificity for natural antimicrobial peptides. No such relationship has been established for synthetic peptoids that mimic antimicrobial peptides. Peptoid macrocycles synthesized with five different aromatic groups are investigated by minimum inhibitory and hemolytic concentration assays, epifluorescence microscopy,...
Thesis
Full-text available
Nature is a major source of inspiration for drug design. Bacteria are developing resistance towards conventional antibiotics. Utilizing antimicrobial peptides (AMPs) – an essential component of innate immune system, as therapeutic agents, may be a viable alternative. Unfortunately, there are a number of serious hurdles on the way towards clinical a...
Article
Full-text available
The peptidomimetic approach has emerged as a powerful tool for overcoming the inherent limitations of natural antimicrobial peptides, where the therapeutic potential can be improved by increasing selectivity and bioavailability. Restraining conformational flexibility of a molecule may reduce the entropy loss upon its binding to the membrane. Experi...
Article
Full-text available
Small hydrophilic antibiotics traverse the outer membrane of Gram-negative bacteria through porin channels. Large lipophilic agents traverse the outer membrane through its bilayer, containing a majority of lipopolysaccharides in its outer leaflet. Genes controlled by the two-component regulatory system PhoPQ modify lipopolysaccharides. We isolate l...
Article
Full-text available
A promising class of potential new antibiotics are the antimicrobial peptides or their synthetic mimics. Herein we assess the effect of the type of cationic side chain (i.e., guanidino vs. amino groups) on the membrane perturbing mechanism of antimicrobial α-peptide-β-peptoid chimeras. Two separate Langmuir monolayers composed of 1,2-dipalmitoyl-sn...
Conference Paper
Full-text available
In this investigation, three complimentary experimental techniques including atomic force microscopy (AFM), X-ray reflectivity (XR), and epiflourescence microscopy (EFM) were employed to determine the mechanism of action of the antimicrobial cyclic peptoid ML2-6 on model mammalian and bacteria membrane systems. Mammalian and bacterial membranes wer...
Conference Paper
Full-text available
Pulmonary surfactant (PS) is the complex mixture of lipids and proteins that forms a thin film on the liquid layer that lines the alveolar air-sacks of the lungs. When compressed by the decreasing alveolar surface area during exhalation, the surfactant films reduce surface tension to exceptionally low levels. This behavior in situ contrasts with th...
Conference Paper
Full-text available
Non-natural oligomeric mimics of antimicrobial peptides (AMPs) can be designed to display chemical moieties analogous to the active side chains of natural peptides, while their abiotic backbone provides protection from proteolytic degradation. N-substituted glycine oligomers (peptoids) are an outstanding example of potential anti-infectious agents...
Conference Paper
Full-text available
Synthetic compounds mimicking the structure of natural antimicrobial peptides (AMPs) have a great promise as potential anti-infectious agents due to their stability towards enzymatic degradation, high antibiotic efficiency, and broad adjustability of physicochemical properties. Recently we have demonstrated that antimicrobial activity of AMP synthe...

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Projects

Projects (4)
Project
Identify molecular targets for novel anticancer peptidomimeitcs
Project
Establish the structure-function relationships in pulmonary surfactant films
Project
Reveal the effect by lipopolysaccharide (LPS) modifications through PhoPQ regulatory system on Salmonella outer membrane permeability