Kjersti E. Lovberg

Norwegian Veterinary Institute · Department of Chemistry and Toxicology

Boronates bind reversibly to vic-diols, a common structural feature of algal toxins. This boronate–diol interaction can be exploited for selective toxin clean-up and concentration. Boric acid gel (BAG) solid phase extraction (SPE) was recently shown to eliminate interferences and matrix effects in LC-MS analyses of azaspiracids (AZAs) in mussel ext...

Microcystins (MCs) are toxic cyclic peptides produced by cyanobacteria worldwide. Due to their hepatotoxicity, it is important to have cheap, rapid and reliable methods for analysis available to protect livestock and people from acute or chronic exposure. A multihapten approach was used to raise new polyclonal antibodies, with the aim of developing...

Immunoaffinity columns are widely used in sample preparation for the analysis of mycotoxins. However, despite the availability of antibodies with broad specificity to many families of algal toxins, little use has been made of these antibodies in immunoaffinity columns. We have developed immunoaffinity columns using antibodies originally produced fo...

Microcystins (MCs) are a group of hepatotoxic heptapeptides that inhibit protein phosphatases, and are produced by many species of cyanobacteria. Most MCs contain the unusual β-amino acid “Adda5 ”, γ-linked D-Glu6, and N-methyldehydroalanine (Mdha 7) at positions 5–7, with more than 250 different microcystin congeners having been reported in the li...

This file contains color photographs of fractions from the three boric acid gel columns, a molecular model of a tetrahedral phenylboronate complex with a 22-desmethyl-23-hydroxyazaspiracid, tabulated amounts of azaspiracid analogues from the fractionated IRNO LRM extract, and graphs showing matrix effects for LC–MS analysis of AZA2 and AZA3 in spik...

Azaspiracids (AZAs) belong to a family of more than 50 polyether toxins originating from marine dinoflagellates such as Azadinium spinosum. All of the AZAs reported thus far contain a 21,22-dihydroxy group. Boric acid gel (BAG) can bind selectively to compounds containing vic-diols or α-hydroxycarboxylic acids via formation of reversible boronate c...

Brown crabs (Cancer Pagurus) were held in tanks and dosed with toxin-free blue mussel homogenate spiked with either pure azaspiracid-1 (AZA1) in 2013 or the cyclic imine pinnatoxin G (PnTxG) in 2014. The crabs were humanely sacrificed periodically up to two weeks after dosing, dissected, and analysed for toxins and metabolites by LC-HRMS. A selecti...

Azaspiracids (AZAs) are a group of biotoxins that cause food poisoning in humans. These toxins are produced by small marine dinoflagellates such as Azadinium spinosum, and accumulate in shellfish. Ovine polyclonal antibodies were produced and used to develop an ELISA for quantitating AZAs in shellfish, algal cells, and culture supernatants. Immuniz...

Wild-caught marine fish are potentially carrying parasites. Larvae of the nematode Anisakis simplex (herring or whale worm) occur in almost all commercially exploited fish stocks in temperate seas. The presence of A. simplex in fish and fish products is not only an economic concern but represents a significant consumer health risk. Anisakiasis, hum...

Microcystins (MCs) are a group of biotoxins (>150) produced by cyanobacteria, with a worldwide distribution. MCs are hepatotoxic, and acute exposure causes severe liver damage in humans and animals. Rapid and cheap methods of analysis are therefore required to protect people and livestock, especially in developing countries. To include as many MCs...

Microcystin-ELISA method description

Supporting information for "Multi-hapten approach leading to a sensitive ELISA with broad cross-reactivity to microcystins and nodularin"

This study focused on a flock of Dala sheep with recurrent cases of chronic copper (Cu) poisoning. The seasonal variation in hepatic Cu concentration was followed in individual sheep with repeated liver biopsies, four times per year, in two consecutive years. Thirty-six ewes were included, yielding a total of 279 biopsies. Cu concentrations were me...

Microcystins (MCs) are a group of biotoxins produced by cyanobacteria from fresh water, with a worldwide distribution in lakes and water reservoirs. MCs are hepatotoxic and acute exposure causes severe liver damage in humans and animals. Rapid and cheap methods of analysis are therefore required to protect people and livestock, especially in develo...

Wild-caught marine fish is potentially carrying parasites. Larvae of the nematode Anisakis simplex (herring or whale worm) occur in virtually all commercially exploited fish stocks in temperate seas. The Anisakis life cycle is complex involving planktonic crustaceans, fish and marine mammals. In fish, the larvae are mainly situated in the visceral...

Celery is acknowledged as a major food allergen in Europe, and mandatory labeling for preprocessed foods has been implemented. However, no methods for the specific detection of celery protein in foods have been published. In the present study, a sandwich celery ELISA using polyclonal anticelery antibodies for capture and detection was developed and...

Sesame (Sesame indicum) has been included into the EU’s list of food allergens for which labelling on pre-packed food products is mandatory. The prevalence of sesame allergy has increased during the last decade. Both sesame seeds and sesame oil have been shown to elicit anaphylactic shock. Threshold doses of respectively 30 mg sesame seeds and 1 ml...

Sesame (Sesame indicum) is considered a potent food allergen because both sesame seeds and sesame oil can elicit anaphylactic reactions in patients with sesame allergy. Threshold doses of respectively 30 mg and 1 ml have been determined in clinical studies. Sesame seeds and products thereof are included in the EU’s list of allergens for which label...

Detection of allergenic proteins in foods is important to ensure safe products for allergic consumers, to help food manufacturers control production and to enable the authorities to assess implementation of food laws. Analytical methods consist of two separate procedures, i.e. the extraction of proteins from the food matrix and the qualitative or q...

Hen's egg white protein is a major cause of food allergy, and a considerable number of countries have introduced labeling directions for processed food products. To control compliance with these regulations, analytical assays for the detection of egg in manufactured foods have been developed. In this study, we have tested the performance of 3 comme...

Hen’s egg is a major food allergen and can cause acute allergic reactions in sensitised persons. The global prevalence of allergy to egg has been calculated at 1.6% in children aged 2.5 years (Eggesbo et al.) Many countries have introduced mandatory labelling of egg in food products. The egg white has a stronger allergic potential than the egg yolk...

Hen’s egg is a major food allergen and can cause acute allergic reactions in sensitised persons. The global prevalence of allergy to egg has been calculated at 1.6% in children aged 2.5 years (Eggesbo et al.) Many countries have introduced mandatory labelling of egg in food products. The egg white has a stronger allergic potential than the egg yolk...

With the aim of lowering the detection limit for casein in foods, three competitive assays are described: direct time-resolved fluoroimmunoassay (TR-FIA), using europium-conjugated antibody, indirect TR-FIA, using biotinylated antibody with europium-conjugated streptavidin and ELISA, using a HRP-conjugated secondary antibody. Food samples (instant...

Aims: Cow’s milk allergy may give severe reactions in infants and adults. The allergenicity and antigenicity of cow’s milk proteins can be altered during food processing and by in vitro digestion and hydrolysis. We have determined the peptic digestion patterns of four caseins in five milk types commercially available in Norway under conditions mimi...