Kiyoshi Tomiyama

• PhD
• Kanagawa Dental University

Purpose: To investigate the antimicrobial effects of a mouthrinse containing silver nanoparticles (AgNP) on polymicrobial biofilms in vitro. Materials and methods: Polymicrobial biofilms were grown on glass cover slips following the method of Exterkate. Saliva collected from a healthy human was added to McBain medium (including 0.2% sucrose) to...

Purpose: To analyze the effects of surface pre-reacted glass-ionomer (S-PRG) filler eluate on polymicrobial biofilm metabolism and live bacterial count. Methods: Biofilm was formed using glass disks 12 mm in diameter and 150 µm in thickness. Stimulated saliva was diluted 50-fold with buffered McBain 2005 and cultured in anaerobic conditions at 3...

We investigated the remineralization effects of Nanoseal (NS) dentin desensitizer on demineralized root dentin. Baseline lesion specimens prepared from bovine root dentin were immersed in artificial saliva (AS) or deionized water (DW) after treatment with NS or fluoride-free Nanoseal (NS(−)). Treatment and control groups comprised: 1, AS; 2, NS/AS;...

This study investigated the anti-demineralization effects of surface pre-reacted glass-ionomer (S-PRG) filler-containing varnishes. Thirty-five bovine root specimens were divided into five treatment groups, with seven specimens each coated with 1) MI varnish (MIV), 2) F varnish (FV), 3) PRG varnish I (PV), 4) PRG varnish II (with sodium fluoride ad...

Purpose: To analyze changes in pH and bacterial flora with duration of culture and timing of sugar supply using a polymicrobial biofilm model. Methods: The biofilm was prepared using the method of Exterkate et al. Stimulated saliva from an adult was collected on a glass slide and added to unbuffered McBain medium containing 0.2% sucrose and cult...

Purpose: To compare the efficacy of toothpaste containing surface pre-reacted glass-ionomer (S-PRG) filler particles to that of conventional sodium fluoride (NaF) toothpaste for the prevention of dentin demineralization and biofilm regrowth. Methods: Bovine root dentin specimens and glass coverslips were used as biofilm growth substrates. To est...

The purpose of this study was to evaluate the adherence and detachment of bacterial biofilms formed on glass surface modified with 10F2S unmodified cover glasses were imme and theywere cultured to develop PM biofilms. There were separated six experimental groups (n = 6), designated WC: cover glass washing withCPW, WI: modified with 10F2S with CPW,...

We used a polymicrobial (PM) biofilm model to examine associations of bacterial adhesiveness with surface characteristics of various dental materials. Four types of dental materials (apatite pellet, zirconia, ceramic, and composite resin) with rough and mirror surfaces were used. Surface roughness, surface free energy, zeta potential, and colony-fo...

The purpose of this study was to evaluate the adherence and detachment of bacterial biofilms formed on glass surface modified with 10F2S unmodified cover glasses were imme and theywere cultured to develop PM biofilms. There were separated six experimental groups (n = 6), designated WC: cover glass washing withCPW, WI: modified with 10F2S with CPW,...

This study investigated the in vitro anti-demineralization effects of resin-based temporary filling materials containing surface prereacted glass-ionomer (S-PRG) filler on dentin. Bovine root dentin specimens with a 3×3 mm experimental surface were divided into four treatment groups: DuraSeal (DU) as a control, S-PRG filler-free temporary material...

The purpose of this study was to evaluate the antibacterial activity against polymicrobial (PM) biofilms of a condensed tannin extracted from astringent persimmon (PS-M), which is contained in refreshing beverages commercially available in Japan. Salivary PM biofilms were formed anaerobically on glass coverslips for 24 and 72 h and were treated for...

To simulate an oral demineralization environment by multiple species of bacteria by inducing subsurface dentin lesions with a polymicrobial biofilm model. Polymicrobial biofilms consisting of multiple species of bacteria were generated from stimulated saliva using a high-throughput active attachment model. Biofilms were grown on dentin specimens in...

To investigate the in vitro antimicrobial effects of carbamide peroxide (CP) and CP-based home bleaching agents against polymicrobial (PM) biofilms. Using a high-throughput active attachment model, PM biofilms were cultured on glass coverslips by diluting the stimulated saliva of one healthy adult. All experiments were performed anaerobically in Mc...

A fluoride-releasing coating material containing surface pre-reacted glass-ionomer (S-PRG) filler has become commercially available. However, there has been no detailed investigation of its remineralization effects at various tooth surface regions. The remineralization effects of S-PRG filler-containing coating material at different sites of demine...

Objective: Surface pre-reacted glass-ionomer (S-PRG) filler shows ability to release fluoride and other ions (Sr2+, SiO32-, BO33-, Na+, and Al+3). We evaluated the antibacterial effects of an eluate from S-PRG filler on polymicrobial oral biofilm in vitro. Methods: S-PRG filler was suspended in distilled water and the saturated clear supernatant us...

Objective: The objective of this study is to examine detachment of the biofilm formed using the polymicrobial biofilm model1) on the glass surface modified with 1H,1H,2H,2H-henicosafluorododecyl-triisocyanatosilane (10F2S-3I). Method: This study is four experimental groups that unmodified group (C), modified group by treated glass cover slips wit...

Objective: PancilPS-M (Rilis Co., Ltd, Osaka, Japan) is a food addictive containing condensed tannin extracted from astringent persimmon. The purpose of this study was to examine the antibacterial action of Pancil PS-M on the formation of polymicrobial biofilms. Methods: PancilPS-M was dissolved in distilled water, sterilized and serially diluted f...

Objectives: Temporary resinous filling materials are frequently used to seal prepared cavities in indirect restorations. Surface pre-reacted glass-ionomer (S-PRG) filler which releases various kinds of ions including fluoride, strontium, sodium, boron, aluminum and silicate, has been reported to show anti-demineralization and antibacterial effects....

To investigate the laboratory anti-demineralization effect of a novel fluoride-releasing varnish containing surface reaction-type prereacted glass-ionomer (S-PRG) filler. Paired specimens were cut from bovine root dentin. One of each pair was used for the S-PRG group, and the other served as a control (n = 6). A 1 x 3 mm test surface was made on ea...

Objectives: It has been reported that a sealing material (Nanoseal) consisted of nano-sized fluoroaluminosilicate glass particle and 10 % phosphoric acid has an ability to occlude opened dentinal tubules. The aim of this study was to investigate the acid resistibility of dentin with Nanoseal by transversal microradiography. Methods: 24 specimens...

Objectives: The purpose of the study was to establish a microcosm biofilm model resulting in dentin carious lesions utilizing saliva derived microcosm biofilms in vitro. Methods: A high-through-put biofilm model was used based on active attachment of bacteria to surfaces (Exterkate et al, Caries Research 2010). Biofilms were formed on either dent...

Objectives: The increase of root caries in the geriatric population has become a crucial problem in developed countries. S-PRG (Surface reaction type Pre-Reacted Glass-ionomer) filler has fluoride release and recharge properties. The aim of this study was to investigate remineralization effect of PRG barrier coat containing S-PRG filler on deminera...

Objective: This study investigated chlorhexidine treatment on and subsequent recovery of saliva derived microcosm biofilms. Methods: A high-through-put model was used based on active attachment of bacteria to surfaces. Biofilms were formed on either dentine or glass specimens. Experiments were performed an-aerobically in McBain medium, which was re...

The effect of fluoride-containing orthodontic resin bonding systems on acid resistance of adjacent enamel was evaluated using transversal microradiography. Four fluoride-containing orthodontic resin bonding systems: BeautyOrtho Bond (BO), BeautyOrtho Bond+Salivatect (BOS), Kurasper F (KP), Transbond XT (TB) and a resin-modified glass-ionomer (Fuji...

This study evaluated the capability of a novel fluoride-releasing, all-in-one adhesive system on forming inhibition layer (radio-opaque layer) as compared with other adhesive systems. Dentin surface was treated with Imperva bond (IB), FL-BOND (FB), Reactmer bond (RE), or FL-BOND S-1 (FS) (which is a novel system). Untreated specimens were categoriz...

The purpose of this study was to evaluate the durability of tubules occluded with FTLA treatment by toothbrush abrasion test on the applied surface and by measuring fluoride release from the FTLA components. Dentin specimens with simulated hypersensitive surfaces were treated with APF containing tannic acid. After which, the specimens received lant...

To simulate hypersensitive dentin, the smear layer and dentinal plugs of bovine root dentin specimens were removed by immersion in 10% phosphoric acid, polishing with hydroxyapatite particles, and ultrasonic cleansing. The fluoride-tannic acid-lanthanum-apatite (FTLA) group was treated with acidulated phosphate fluoride (APF) containing tannic acid...

われわれはMMP-3 (ストロメライシン) 様活性を持つ59KDのプロテアーゼ (59KDP-ase) を象牙質から抽出したことを報告してきた。この研究の目的は象牙質プロテオグリカン (PG) を抽出し, 59KP-aseによる分解特性を調べることである。PGはウシ象牙質から4MGuHCl (Gl-ext), 0.5MEDTA (E-ext) と4MGuHCL (G2-ext) を用いて連続抽出した。各抽出画分からDEAES ephacel, Superose6とReversed phasedFPLCを用いてPGを精製した。これらPGの分子量はSDS/PAGE電気泳動でGl-extは150～180KDと300KD, E-extは130～150KD, またG2-extは130～150KDと18...

Objectives: Surface reaction type Pre-Reacted Glass-ionomer (S-PRG) powder has the ability to release and recharge fluoride effectively. Therefore it might be expected that when the powder occlude dentinal tubules, the released fluoride will induce mineral deposition in the tubules together with calcium and phosphate from saliva. The aim of this st...