Kiwamu Tanaka

Kiwamu Tanaka
Washington State University | WSU · Department of Plant Pathology

Ph.D.

About

77
Publications
21,656
Reads
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3,375
Citations
Citations since 2017
35 Research Items
2416 Citations
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20172018201920202021202220230100200300400500
20172018201920202021202220230100200300400500
20172018201920202021202220230100200300400500
Introduction
Kiwamu Tanaka currently works at the Department of Plant Pathology, Washington State University, where he is mainly teaching molecular genetics in plant-pathogen interaction.
Additional affiliations
July 2020 - present
Washington State University
Position
  • Professor (Associate)
June 2014 - June 2020
Washington State University
Position
  • Professor (Assistant)
October 2011 - May 2014
University of Missouri
Position
  • Researcher
Education
April 2002 - September 2005
Kagoshima University
Field of study
April 2000 - March 2002
Kagoshima University
Field of study
April 1996 - March 2000
Kagoshima University
Field of study

Publications

Publications (77)
Article
Danger signals trigger immune responses upon perception by a complex surveillance system. Such signals can originate from the infectious nonself or the damaged self, the latter termed damage-associated molecular patterns (DAMPs). Here, we apply Matzinger’s danger model to plant innate immunity to discuss the adaptive advantages of DAMPs and their i...
Article
Full-text available
Calcium and reactive oxygen species (ROS) are two of the earliest second messengers in response to environmental stresses in plants. The rise and sequestration of these messengers in the cytosol and apoplast are formed by various channels, transporters, and enzymes that are required for proper defense responses. It remains unclear how calcium and R...
Article
Because symptoms of plant diseases are often ambiguous for identifying the causal pathogen, chemical applications and cultural managements are occasionally applied against the wrong target and/or at the wrong time. Some diseases are difficult to diagnose due to latent or absent characteristic symptoms. For example, diseases caused by soil-borne pat...
Article
Full-text available
Plant pathogens degrade cell wall through secreted polygalacturonases (PGs) during infection. Plants counteract the PGs by producing PG-inhibiting proteins (PGIPs) for protection, reversibly binding fungal PGs, and mitigating their hydrolytic activities. To date, how fungal pathogens specifically overcome PGIP inhibition is unknown. Here, we report...
Article
Full-text available
Extracellular ATP (eATP) is a purinergic signal recognized by plasma membrane-localized transmembrane receptors, such as P2X or P2Y receptors found in mammals, and the P2K receptors found in plants. In mammals, eATP and purinoceptors are the basis of intercellular signaling used to regulate diverse processes including neuronal signaling, apoptosis,...
Article
Full-text available
Timely detection of plant pathogens enables a suitable, effective control strategy to be implemented. Many potato growers adjust their management strategies based on disease diagnosis. However, in some cases disease diagnosis comes too late to manage within a growing season. Rapid and accurate technologies are required for early pathogen detection.
Article
Full-text available
Calcium (Ca2+) signaling in plant cells is an essential and early event during plant-microbe interactions. The recognition of microbe-derived molecules activates Ca2+ channels or Ca2+ pumps that trigger a transient increase in Ca2+ in the cytoplasm. The Ca2+ binding proteins (such as CBL, CPK, CaM, and CML), known as Ca2+ sensors, relay the Ca2+ si...
Article
Calcium (Ca²⁺) signaling regulates salicylic acid (SA)-mediated immune response through calmodulin-meditated transcriptional activators, AtSRs/CAMTAs, but its mechanism is not fully understood. Here, we report an AtSR1/CAMTA3-mediated regulatory mechanism involving the expression of the SA receptor, NPR1. Results indicate that the transcriptional e...
Article
The role of small secreted peptides in plant defense responses to viruses has been seldom investigated. Here, we report a role for potato (Solanum tuberosum) PIP1, a gene predicted to encode a member of the PAMP-induced peptide (PIP) family, in the response of potato to potato virus Y infection (PVY). We show that exogenous application of synthetic...
Preprint
Full-text available
Calcium signaling regulates salicylic acid (SA)-mediated immune response through calmodulin-meditated transcriptional activators, AtSRs/CAMTAs, but its mechanism is not fully understood. Here, we report an AtSR1/CAMTA3-mediated regulatory mechanism involving the expression of the SA receptor, NPR1. Transcriptional expression of NPR1 increased in kn...
Article
Full-text available
Plants encrypt the perception of different pathogenic stimuli into specific intracellular calcium (Ca2+) signatures and subsequently decrypt the signatures into appropriate downstream responses through various Ca2+ sensors. Two microbe-associated molecular patterns (MAMPs), bacterial flg22 and fungal chitin, and one damage-associated molecular patt...
Article
Full-text available
The purinoceptor P2K1/DORN1 recognizes extracellular ATP, a damage-associated molecular pattern (DAMP) released upon cellular disruption by wounding and necrosis, which in turn, boost plant innate immunity. P2K1 is known to confer plant resistance to foliar biotrophic, hemi-biotrophic, and necrotrophic pathogens. However, until now, no information...
Article
Full-text available
The first layer of plant immunity is deployed by recognition of pathogen-associated molecule patterns (PAMPs) and induction of early stress responses. Flagellin is the major protein component of the flagellum. Flagellin-derived peptide fragments, such as Flg22, a short active peptide derived from the highly conserved part of the N-terminal region,...
Article
Full-text available
The amplification of specific nucleic acid sequences with high specificity and sensitivity is an essential technique for pathogen detection. Recombinase polymerase amplification (RPA) is a rapid isothermal amplification method. Here, we demonstrate the end-point and real-time detection of Spongospora subterranea f. sp. subterranea (Sss) using RPA a...
Article
Full-text available
Extracellular ATP is perceived by the purinoceptor P2K1, leading to induction of defense response in plants. Previously, we described the transcriptomic response to extracellular ATP in wild-type Arabidopsis seedlings and mutants of classical defense hormone signaling pathways (Jewell et al., 2019, Plant Physiol. 179: 1144–58), in which extracellul...
Chapter
Extracellular ATP functions as an important signaling molecule in both plants and animals. In plants, ATP is released in the extracellular region of cells in response to environmental perturbations, such as herbivory, cellular damage, or other abiotic and biotic stimuli, which is then perceived by the purinoceptor P2K1 as a damaged-self signal for...
Article
Second messengers are cellular chemicals that act as “language codes”, allowing cells to pass outside information to the cell interior. The cells then respond through triggering downstream reactions, including transcriptional reprograming to affect appropriate adaptive responses. The spatiotemporal patterning of these stimuli-induced signal changes...
Article
Full-text available
ATP is not only an essential metabolite of cellular biochemistry but also acts as a signal in the extracellular milieu. In plants, extracellular ATP is monitored by the purinergic receptor P2K1. Recent studies have revealed that extracellular ATP acts as a damage-associated molecular pattern in plants, and its signaling through P2K1 is important fo...
Article
Full-text available
Over the last decades, medical research has utilized DNA altering procedures in cancer treatments with the objective of killing cells or suppressing cell proliferation. Simultaneous research related to enhancing disease resistance in plants reported that alterations in DNA can enhance defense responses. These two opposite perspectives have in commo...
Article
Full-text available
Calcium signal plays a fundamental role in plant immune responses. Various calcium sensors have been characterized to relay and/or decode pathogen-induced calcium signals. Autoimmune mutants are helpful resources for the dissection of plant immune systems which have allowed researchers to access in-depth mechanisms of defense signaling. Recent disc...
Article
Full-text available
On-site diagnosis of plant diseases can be a useful tool for growers for timely decisions enabling the earlier implementation of disease management strategies that reduce the impact of the disease. Presently in many diagnostic laboratories, the polymerase chain reaction (PCR), particularly real-time PCR, is considered the most sensitive and accurat...
Article
Full-text available
Damage-associated molecular patterns (DAMPs), such as extracellular ATP, act as danger signals in response to biotic and abiotic stresses. Extracellular ATP is perceived by a plant purinoceptor, P2 receptor kinase 1 (P2K1), inducing downstream signaling for defense responses. How ATP induces these defense responses has not been well studied. A rece...
Article
Full-text available
Damaged cells send various signals to stimulate defense responses. Recent identification and genetic studies of the plant purinoceptor, P2K1 (also known as DORN1), have demonstrated that extracellular ATP is a signal involved in plant stress responses, including wounding, perhaps to evoke plant defense. However, it remains largely unknown how extra...
Article
Plant recognition of invading organisms occurs through identification of foreign molecules associated with attackers and of self-derived, damage-associated molecules. Perception of these molecules activates signaling processes including dynamic changes in ion balance, production of second messengers such as reactive oxygen species and nitric oxide,...
Article
Full-text available
Phytoalexins are antimicrobial substance synthesized in plants upon pathogen infection. Pisatin (Pisum sativum phytoalexin) is the major phytoalexin in pea, while it is also a valuable indicator of plant defense response. Pisatin can be quantitated in various methods from classical organic chemistry to Mass-spectrometry analysis. Here we describe a...
Article
Full-text available
Salicylic acid (SA) has been reported to induce plant defense responses. The transcriptions of defense genes that are responsible for a given plant’s resistance to an array of plant pathogens are activated in a process called non-host resistance. Biotic signals capable of carrying out the activation of pathogenesis-related (PR) genes in pea tissue...
Poster
Full-text available
On-site diagnosis of plant disease is pivotal for growers as timely decisions regarding early-stage treatment are often crucial and can thereby reduce secondary spread of disease in the field. Polymerase chain reaction (PCR) is currently the most sensitive and accurate method for plant pathogen detection. However, standard PCR requires expensive la...
Article
Full-text available
The defense gene activations that provide non-host disease resistance in plants are signaled by various elicitors. A number of the fungal-derived signal such as chitosan and DNase are uniquely able to promote transcription through direct effects on the DNA of pea chromatin at sites of defense gene transcription. Other biological signals are contain...
Article
Full-text available
A quantitative and robust bioassay to assess plant defense response is important for studies of disease resistance and also for the early identification of disease during pre- or non-symptomatic phases. An increase in extracellular pH is known to be an early defense response in plants. In this study, we demonstrate extracellular alkalinization as a...
Article
Full-text available
Fungi that cause cereal rust diseases (genus Puccinia) are important pathogens of wheat globally. Upon infection the fungus secretes a number of effector proteins. Although a large repository of putative effectors have been predicted using bioinformatic pipelines, the lack of available high-throughput effector screening systems has limited function...
Article
Full-text available
DORN1 (also known as P2K1) is a plant receptor for extracellular ATP, which belongs to a large gene family of legume-type (L-type) lectin receptor kinases. Extracellular ATP binds to DORN1 with strong affinity through its lectin domain, and the binding triggers a variety of intracellular activities in response to biotic and abiotic stresses. Howeve...
Data
A typical design of grid coverage in 3D spaces for docking experiments of DORN1 and ATP. (A) The 3D grid space covered the DORN1 model entirely for free docking experiments, i.e. allowing ATP targets to any potential pocket on the DORN model. (B) The grid box stationed on the predicted binding site made of 4 defined loops A-D. The grid box and dock...
Data
Graphical representation of the template binding site for GalNAc (brown) and FD (light blue) ligands. (A) Ribbon and (B) molecular surface representations of the template?s binding sites for GalNAc ligand in the crystal complex (brown) and the docking model (light blue). (C) Ribbon and (D) molecular surface representations of the template?s binding...
Data
Fold prediction results of DORN1 L-type lectin domain using the sequence-structure homology recognition method. (DOCX)
Data
Search statistics of binding sites for DORN1 using sequence and structure-based prediction tools. (DOCX)
Data
Number of rotatable bonds, hydrogen bonds, hydrophobic interactions and binding affinities of DORN1 and ligands. (DOCX)
Data
Graphical representations of the DORN1 model optimization steps and quality evaluation in term of energy. (A) The atomic distance variation in terms of root mean square deviation (RMSD) between corresponding aligned atoms in angstrom scale generated during 20ps (picoseconds) of molecular dynamic simulation. (B) Total potential energy of the model s...
Data
Stereo-chemical quality of DORN initial and refined models, and the best template structure 3IPV-chain A. (DOCX)
Data
Ramachandran plots of the DORN1 model structure validation. (A) Graphical representation of alpha-carbon geometry (phi-?, psi-?) and beta-carbon deviation values of the DORN1 model before refinement (B) Graphical representation of alpha-carbon geometry (phi-?, psi-?) and beta-carbon deviation values of the DORN1 model after refinement. Black dots d...
Data
Structural alignment of DORN1 and 14 top rank legume lectins. ARC1 (Arcelin 1)-Phaseolus vulgaris (PDB code: 1AVB), DBL-Dolichos biflorus (1BJQ), MAL-Maackia amurensis (1DBN), WBA2-Dolichos tetragonolobus (1F9K), DLEC2-Phaseolus vulgaris (1FAT), ECL-Erythrina crista-galli (1GZ9), GSI-B4-Griffonia simplicifolia (1HQL), LE1-Glycine max (1SBF), WBAI-D...
Data
In silico binding affinity (-kcal/mol) of different ligands to DORN1 and the template DBL. Binding affinity values of nucleotides and sugars to (A) the DORN1 model and (B) the template crystal model (DBL) obtained from target docking experiments. Lac: lactose, Gal: galactose, FD: Forssman disaccharide (GalNAc(?1?3)GalNAc), A2G: N-Acetyl Galactosami...
Chapter
Ectoapyrases are nucleotide hydrolyzing enzymes composed of a transmembrane domain and an extracellular catalytic domain. These enzymes are thought to control the extracellular ATP concentration, which is important for plant growth, development, and response to stress. Recent studies have clearly shown that the enzymatic activity of ectoapyrase is...
Article
Full-text available
Although lipo-chitooligosaccharides (LCOs) are important signal molecules for plant-symbiont interactions, a number of reports suggest that LCOs can directly impact plant growth and development, separate from any role in plant symbioses. In order to investigate this more closely, maize and Setaria seedlings were treated with LCO and their growth wa...
Article
Full-text available
Pea pod endocarp suppresses the growth of an inappropriate fungus or non-pathogen by generating a "non-host resistance response" that completely suppresses growth of the challenging fungus within 6 h. Most of the components of this resistance response including pisatin production can be elicited by an extensive number of both biotic and abiotic ind...
Article
Full-text available
Chitin is a fungal microbe-associated molecular pattern recognized in Arabidopsis by a lysin motif receptor kinase (LYK), AtCERK1. Previous research suggested that AtCERK1 is the major chitin receptor and mediates chitin-induced signaling through homodimerization and phosphorylation. However, the reported chitin binding affinity of AtCERK1 is quite...
Article
Full-text available
ATP, the universal energy currency of all organisms, is released into the extracellular matrix and serves as a signal among cells, where it is referred to as an extracellular ATP. Although a signalling role for extracellular ATP has been well studied in mammals over the last 40 years, investigations of such a role in plants are at an early stage. R...
Article
Chitin is the second most abundant polysaccharide in nature, found in crustacean shells, insect exoskeletons and fungal cell walls. The action of chitin and chitin derivatives on plants has become a very interesting story of late. Chitin is a β1-4-linked polymer of N-acetyl-d-glucosamine (GlcNAc). In this unmodified form, chitooligosaccharides (deg...
Article
Full-text available
As sessile organisms, plants have evolved effective mechanisms to protect themselves from environmental stresses. Damaged (i.e., wounded) plants recognize a variety of endogenous molecules as danger signals, referred to as damage-associated molecular patterns (DAMPs). ATP is among the molecules that are released by cell damage, and recent evidence...
Article
Because of their sessile nature, plants have developed a number of sophisticated signaling systems to adapt to environmental changes. Previous research has shown that extracellular ATP is an important signaling molecule used by plants and functions in a variety of processes, including growth, development, and stress responses. Recently, DORN1 was i...
Article
Full-text available
Extracellular adenosine 5′-triphosphate (ATP) is an essential signaling molecule that is perceived in mammals by plasma membrane P2-type purinoceptors. Similar ATP receptors do not exist in plants, although extracellular ATP has been shown to play critical roles in plant growth, development, and stress responses. Here, we identify an ATP-insensitiv...
Article
Full-text available
Legumes (Fabaceae) establish a symbiotic relationship with nitrogen fixing soil bacteria (rhizobia) to overcome nitrogen source limitation. Single root hair epidermal cells serve as the entry point for bacteria to infect the host root leading to development of a new organ, the nodule, which the bacteria colonize. In the present study, the putative...
Article
Full-text available
Stealth Nod Factor Recognition Legumes' symbiotic interaction with nitrogen fixing bacteria supplies the plant with nitrogen. Many important crop plants, however, cannot establish these symbioses and, thus, agriculture depends on externally applied fertilizers. Surprisingly, Liang et al. (p. 1384 , published online 5 September) found that several n...
Article
Full-text available
Heterotrimeric GTP-binding proteins (G-proteins) and G-protein-coupled receptors are important signaling components in eukaryotes. In plants, the G-proteins are involved in diverse physiological processes, some of which are exerted via changes in the level of cytosolic free calcium concentration ([Ca(2+)]cyt). Various techniques have been developed...
Article
Full-text available
Recent research findings clearly indicate that lysin motif (LysM)-containing cell surface receptors are involved in the recognition of specific oligosaccharide elicitors (chitin and peptidoglycan), which trigger an innate immunity response in plants. These receptors are either LysM-containing receptor-like kinases (LYKs) or LysM-containing receptor...
Article
Full-text available
Root hairs are single hair-forming cells on roots that function to increase root surface area, enhancing water and nutrient uptake. In leguminous plants, root hairs also play a critical role as the site of infection by symbiotic nitrogen fixing rhizobia, leading to the formation of a novel organ, the nodule. The initial steps in the rhizobia-root h...
Article
Full-text available
Chitin is commonly found in fungal cell walls and is one of the well-studied microbe/pathogen-associated molecular patterns. Previous studies showed that lysin motif (LysM)-containing proteins are essential for plant recognition of chitin, leading to the activation of plant innate immunity. In Arabidopsis (Arabidopsis thaliana), the LYK1/CERK1 (for...
Presentation
Nitrogen fixation in legume root nodules is very important for their agricultural performance and is beneficial to the environment. Apyrases are divalent cation-dependent, tri- and di-nucleotide phosphatases that are ubiquitously found in eukaryotes and prokaryotes. We are studying the soybean ecto-apyrase GS52, which is expressed rapidly upon rhiz...
Article
Full-text available
The expression of DWARF4 (DWF4), which encodes a C-22 hydroxylase, is crucial for brassinosteroid (BR) biosynthesis and for the feedback control of endogenous BR levels. To advance our knowledge of BRs, we examined the effects of different plant hormones on DWF4 transcription in Arabidopsis thaliana. Semi-quantitative reverse-transcriptase PCR show...
Article
Full-text available
Root nodulation is regulated by a variety of mechanisms. Ecto-apyrase is an enzyme proposed to control the concentration of extracellular nucleotides. Transgenic expression of the soybean GS52 ecto-apyrase was shown to stimulate nodulation. However, mutation of the enzyme to disrupt enzymatic activity prevented this effect. Therefore, the data sugg...
Article
Full-text available
Nitrogen is an essential nutrient for plant growth. In the Rhizobium-legume symbiosis, root nodules are the sites of bacterial nitrogen fixation, in which atmospheric nitrogen is converted into a form that plants can utilize. While recent studies suggested an important role for the soybean (Glycine max) ecto-apyrase GS52 in rhizobial root hair infe...
Data
Full-text available
Extracellular ATP is essential for thigmotropic growth control during obstacle avoidance in plant roots, in which a G protein complex is involved in a time-dependent reset of ATP release. In this issue, Tanaka et al. (pages 601–608) describe how extracellular ATP signaling is involved in various processes in plant growth and development. Cover imag...