Kiomars Rouhrazi

To assess the diversity of Rhizobium (Agrobacterium) species and strains, associated with crown gall disease of grapevine (Vitis vinifera) in the major grapevine growing areas of northern Iran, 105 strains of the suspected bacterium were isolated from tumors of the crown and vines collected from vineyards in the East and West Azarbayjan and Ardabil...

Identification and genetic diversity of 44 strains from three pathovars of Xanthomonas arboricola isolated from peach, plum, hazelnut, and walnut in Iran were assessed based on phenotypic features, rep-PCR (ERIC-, BOX-, and rep-PCR) analysis, and sequencing of 16S rRNA, ropD, and gyrB genes. All strains were Gram-negative and produced yellow pigmen...

Background and Objectives The bacterial rot of pepper, onion, and potato is one of the most crucial diseases in the East and West Azerbaijan provinces of Iran. Therefore, accurate identification of the causes of these diseases and the study of their genetic diversity can be beneficial in preventing their occurrence. In the present study, in additio...

Stem and pith necrosis symptoms on tomato and midrib rot symptoms on lettuce were observed in West and East Azarbayjan of Iran. Infected organs of the plants were collected and bacterial strains were isolated on NA medium. Biochemical and physiological properties such as LOPAT test, fluorescent pigment production and utilization of carbon source, d...

garlic soft rot

The diversity and phylogeny of 30 rhizobia isolated from nodules of faba bean plants grown on 5 geographic regions located in the East Azerbaijan province of Iran were examined using rep-PCR fingerprinting, sequence analysis of 16S rRNA accompanied with nodC genes. Based on cluster analysis of rep-PCR fingerprints, faba bean rhizobia isolates were...

Garlic, a commonly grown herb in the garden, is one of the most important and valuable products in Iran with a cultivation area of about 3000 ha. However garlic is also prone to several soil-born diseases. In 2014, a soft rot disease was observed on the bulb base of garlic in Hamadan province of Iran. Bacterial colonies were isolated from symptomat...

From 33 Iranian fluorescent Pseudomonas isolates originating from symptomatic tissues of peach (Prunus persica), plum (Prunus domestica), sweet (Prunus avium) and sour cherry (Prunus cerasus), 27 were identified as Pseudomonas syringae using LOPAT tests. Further characterization of those isolates by GATTa and L‐lactate utilization tests and the det...

Genetic diversity and population structure among 29 isolates of Ascochyta rabiei (AR) obtained from diseased chickpea plants in six different geographical origins in Iran was characterized by MAT and rep-PCR (BOX/ERIC/REP) markers. Both mating types were found in all six populations, and the frequencies of mating types were variable between populat...

In 2012, a leaf spot disease on walnut seedlings was observed in Hamedan province of Iran. The spots were necrotic with yellow halos. Symptomatic samples were collected and suspected bacterial agent was isolated on nutrient agar medium. Phenotypic characteristics such as production of fluorescent pigment on KB medium, LOPAT test and utilization of...

Chickpea (Cicer arietinum), a cool season grain legume grown on a surface of 11 million ha with worldwide production of about 9 million tons, is cultivated in more than 45 countries throughout the world including Iran where it is grown mostly in rainfed areas and on marginal lands in the province of West Azarbayjan. Ascochyta blight is perhaps the...

The phylogenetic diversity of twenty nine rhizobial strains nodulating Phaseolus vulgaris in Iran was analysed on the basis of their core and symbiotic genes. These strains displayed five 16S rRNA-RFLP patterns and belong to eight ERIC-PCR clusters. The phylogenetic analyses of 16S rRNA, recA and atpD core genes allowed the identification of severa...

Forty-five isolates from root nodules of chickpea (Cicer arietinum L.) growing in the western and northwestern regions of Iran were characterized by phenotypic properties, repetitive element sequence-based (rep)-PCR, and 16S rRNA sequences. Based on phenotypic features, the isolates could be divided into three main groups at 92 % similarity. Nodula...

The aim of this study was characterized Pectobacterium carotovorum subsp. carotovorum (Pcc) the causal pathogen of watermelon soft rot disease in Iran. Of fifty bacterial isolates with white grey and convex colonies on nutrient agar obtained from symptomatic watermelon, ten isolates were selected for further tests. Pathogenicity tests results showe...

Iran is the 4th largest producer of watermelon (Citrullus lanatus) in the world. In 2012 a disease characterized by water-soaked lesions and soft rot was observed on mature and immature fruits of watermelon cv. Crimson sweet. Fruit samples with transpicuous symptoms were transferred to laboratory and bacterial colonies were isolated from these on n...

Northern Iran has one of the largest and most diverse populations of cultivated crucifers in Iran. Symptoms of black rot disease were observed in 40 % of fields. To assess the genetic diversity of Xanthomonas campestris pv. campestris (Xcc) strains, associated with black rot disease, 40 strains were isolated from infected samples of crucifers such...

In 2009–2010, crown tumours were collected from walnut (Juglans regia L.) trees in northern Iran. Gram-negative, rod shaped and aerobic bacteria with circular, convex and white-coloured colonies on potato dextrose agar plus CaCO3 medium were isolated from galls. In pathogenicity tests, tomato seedlings were inoculated with all strains and tumours...

Crown gall is amongst the most important diseases of grapevine (Vitis vinifera) in many areas where grapevine is grown worldwide. To assess the diversity of Rhizobium species and strains, associated with the crown gall disease of grapevine in the major grapevine growing areas of northern Iran, 105 strains of the suspected bacterium were isolated fr...

Since 2008, a disease consisting of brown to black angular to irregular spots, 2-4 mm in diameter and surrounded by a chlorotic halo, was observed in northern Iran on the leaves of sleeping hibiscus (Malvaviscus penduliflorus), a widely cultivated ornamental shrub. Bacterial colonies were isolated from symptomatic leaves on nutrient agar containing...

To assess the diversity of Rhizobium (Agrobacterium) species and strains, associated with crown gall disease of grapevine (Vitis vinifera) in the major grapevine growing areas of northern Iran, 105 strains of the suspected bacterium were isolated from tumors of the crown and vines collected from vineyards in the East and West Azarbayjan and Ardabil...

Crown gall of grapevine is an economically important disease in all areas where grapes are grown, but it is particularly severe in regions with cold climates. The purpose of this study was twofold: (1) to compare available Agrobacterium primer sets and identify a more reliable PCR analysis that can detect tumorigenic and non-pathogenic A. vitis and...

Crown gall of grapevine is amongst the most important diseases in many areas where grapevine are grown world wide. The severity of the disease and the losses inflicted are considerably higher under temperate to cold climates. Identification of the inciting species and the genetic structures of the population one of utmost importance for the implame...

To assess the genetic diversity of Rhizobium(Agrobacterium) strains inciting tumors on crowns and roots of various host species, 25 strains were isolated from galls on grapevine(Vitis vinifera), Benjamin's Fig(Ficus benjamina) and walnut (Juglans regia) in East Azarbaijan, Golestan and Mazandaran provinces in 2009-2010. The heterogeneity of the str...