Kingsley Badu

Kingsley Badu
Kwame Nkrumah University Of Science and Technology | KNUST · Department of Theoretical and Applied Biology

PhD Biological Sciences (Immuno - biology)
We are studying transmission of Malaria, Cutaneous leishmaniasis and Trypanosomiasis www.vectorgenes.com

About

63
Publications
19,436
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542
Citations
Citations since 2017
41 Research Items
396 Citations
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Introduction
I am looking at the transcript and the expression profiles ookinete and sporozoite staged invasion proteins (the so called glideosome) of Plasmodium falciparum (in the case of malaria) to elucidate their functional interactions in the invasion process. My goal is to identify biomarkers of infectious bites capable of assessing the human-parasite-vector interactions in order to develop sero-surveillance tools for estimating transmission intensity and transmission blocking vaccines
Additional affiliations
May 2013 - December 2015
Garden City University College
Position
  • Lecturer
Description
  • Teaching and research in Cell Biology, Molecular diagnostics, Medical entomology, microbiology and Immunological defense mechanisms
February 2012 - present
Noguchi Memorial Institute for Medical Research
Position
  • Post-Doctoral Research Fellowship
Description
  • Developing innovative sero-epidemiological tools for malaria transmission intensity estimation
October 2010 - November 2011
University of California, Irvine
Position
  • Researcher
Education
September 2003 - June 2007
Kwame Nkrumah University Of Science and Technology
Field of study
  • Clinical Microbiology

Publications

Publications (63)
Article
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A new database of the Entomological Inoculation Rate (EIR) is used to directly link the risk of infectious mosquito bites to climate in Sub-Saharan Africa. Applying a statistical mixed model framework to high-quality monthly EIR measurements collected from field campaigns in Sub-Saharan Africa, we analyzed the impact of rainfall and temperature sea...
Article
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In this work, we have developed a paper-based microfluidic device capable of remote biofluid collection followed by an analysis of the dried clinical samples using a miniature mass spectrometer. We have evaluated a portable mass spectrometer as a possible surveillance platform by analyzing the clinical malaria samples (whole blood) collected from G...
Article
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Coronavirus disease 2019 (COVID-19) contact tracing and malaria reactive case detection (RACD) are effective strategies for disease control. The emergence of the COVID-19 pandemic and the global attention COVID-19 has received in the recent past and present has hampered malaria control efforts. Among these are difficulties in finding and treating m...
Article
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Monkeypox is an emerging zoonotic disease caused by the monkeypox virus, which is an infectious agent belonging to the genus Orthopoxvirus. Currently, commencing from the end of April 2022, an outbreak of monkeypox is ongoing, with more than 43,000 cases reported as of 23 August 2022, involving 99 countries and territories across all the six World...
Preprint
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Monkeypox is an emerging zoonotic disease caused by the monkeypox virus, which is an infectious agent belonging to the family of Poxviruses. Currently, commencing from the end of April 2022, an outbreak of monkeypox is ongoing, with more than 25,000 cases being reported as of August 3, 2022, involving 90 countries and territories across all the six...
Article
Rapid diagnostic tests (RDTs) are a key tool for the diagnosis of malaria infections among clinical and subclinical individuals. Low-density infections, and deletions of the P . falciparum hrp2 / 3 genes (encoding the HRP2 and HRP3 proteins detected by many RDTs) present challenges for RDT-based diagnosis. The novel Rapigen Biocredit three-band Pla...
Article
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Most rapid diagnostic tests for Plasmodium falciparum malaria target the Histidine-Rich Proteins 2 and 3 (HRP2, HRP3). Deletions of the hrp2 and hrp3 genes result in false negative tests and are a threat for malaria control. A novel assay for molecular surveillance of hrp2/hrp3 deletions was developed based on droplet digital PCR (ddPCR). The assay...
Article
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Nanomedicine strategies were first adapted and successfully translated to clinical application for diseases, such as cancer and diabetes. These strategies would no doubt benefit unmet diseases needs as in the case of leishmaniasis. The latter causes skin sores in the cutaneous form and affects internal organs in the visceral form. Treatment of cuta...
Preprint
Full-text available
Rapid diagnostic tests (RDTs) are a key tool for the diagnosis of malaria infections among febrile and subclinical individuals. Low-density infections, and deletions of the P. falciparum hrp2 / 3 genes (encoding for the HRP2 and HRP3 proteins detected by many RDTs) present challenges for RDT-based diagnosis. The novel Rapigen Biocredit three-band P...
Preprint
Full-text available
Background: The World Health Organization recommends parasitological confirmation of all suspected malaria cases by microscopy or rapid diagnostic tests (RDTs) before treatment. These conventional tools are widely used for point-of-care diagnosis in spite of their poor sensitivity at low parasite density. Several studies have compared the diagnosti...
Article
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In Burundi, malaria infection has been increasing in the last decade despite efforts to increase access to health services, and several intervention programs. The use of heterogeneous data can help to build predictive models of malaria cases. We built predictive frameworks: the generalized linear model (GLM), and artificial neural network (ANN), to...
Preprint
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Background: Leishmaniasis is a vector borne disease caused by an intracellular protozoan parasite. The presence of secondary bacterial infections in cutaneous leishmaniasis wounds exacerbate lesion development and could lead to delay in the healing process. Little is also known about the different bacteria species co-infecting leishmaniasis wounds...
Article
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The novel coronavirus disease 2019 (COVID-19) is one of the biggest public health crises globally. Although Africa did not display the worst-case scenario compared to other continents, fears were still at its peak since Africa was already suffering from a heavy load of other life-threatening infectious diseases such as HIV/AIDS and malaria. Other f...
Article
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Introduction Recent surge of Anopheles resistance to major classes of World Health Organization (WHO)-approved insecticides globally necessitates the need for information about local malaria vector populations. It is believed that insecticide efficacy loss may lead to operational failure of control interventions and an increase in malaria infection...
Preprint
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Malaria in pregnancy remains a major problem of public health concern in Sub-Saharan Africa due to its endemicity and the diverse consequences on both the mother and the baby. Much attention, therefore, is needed to fully understand the epidemiology of the disease and to mitigate the devastating outcomes. The present study aimed at investigating ma...
Preprint
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The African Animal Trypanosomiasis (nagana) is caused by several species of trypanosome species which results in significant clinical diseases. This consequently leads to huge economic losses. We carried out a cross-sectional survey to investigate the composition of vectors, parasite diversity and intensities in two districts in the Eastern region...
Preprint
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Malaria is a major public health concern in Burundi. The infection has been increasing in the last decade despite efforts to increase access to health services, and the deployment of several intervention programs. The use of different data sources can help to build predictive models of malaria cases in different sub-populations. We built predictive...
Preprint
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Background: The most commonly used Plasmodium falciparum rapid diagnostic tests target the Histidine-Rich Proteins 2 and 3 (HRP2, HRP3). An increasing number of countries report parasites that carry hpr2 and/or hrp3 gene deletions, resulting in false negative test results. Molecular surveillance of hrp2 and hrp3 deletions is crucial but adequate pr...
Article
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Coronavirus disease 2019 (COVID-19), a severe acute respiratory syndrome caused by SARS-CoV-2 has been declared a global pandemic by the World Health Organization (WHO) in March 2020. As of 21st April 2021, the disease had affected more than 143 million people with more than 3million deaths worldwide. Urgent effective strategies are required to con...
Article
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The evolving nature of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has necessitated periodic revisions of COVID-19 patient treatment and discharge guidelines. Since the identification of the first COVID-19 cases in November 2019, the World Health Organization (WHO) has played a crucial role in tackling the country-level pandemi...
Chapter
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As countries move from intense malaria transmission to low transmission there will be a demand for more sensitive tools and approaches in tracking malaria transmission dynamics. Surveillance tools that are sensitive in tracking real time infectious bites as well as infectious reservoir will be preferred to counting number of cases in the hospital o...
Article
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This paper aims at provoking broad-based dialogues and debates on ways and means of securing Africa’s health sovereignty. It argues that health sovereignty is about the realization of specific national constitutional and policy objectives on citizens’ access to and enjoyment of good health, resilient to COVID-19 and related disease pandemics. The p...
Article
This cross-sectional study seeks to investigate the bacterial contamination of poultry products (eggs) produced from selected farms and sold within the major markets in the New Juaben Municipality in the Eastern region of Ghana. A total of 100 eggs were sampled randomly from October to December, 2018 from five poultry farms and five retail markets...
Article
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The unforeseeable global crisis of the spread of coronavirus disease 2019 (COVID-19) has caused almost all affected countries to adopt a range of protective measures as recommended by the World Health Organization. However, the speed, type and level of adoption of these protective measures have been remarkably different. Social distancing and quara...
Article
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Background: We aimed at investigating the impact of malaria on the haematological parameters of residents from different demographic settlements in the Ashanti Region of Ghana. Malaria parasites trigger changes in certain haematological parameters, which may result in a number of clinical manifestations. Differences in demographic settlements, such...
Article
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This study compares the bacteriological and sensory characteristics of' fufu' processed using grinding machines and traditional method at Ayigya, a suburb of Kumasi between December 2018 and February, 2019. Samples were collected from three different sites for fufu grinding machine and three sampling areas for traditional method using mortar and pe...
Article
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Background: Toxoplasma gondii is an obligate, intracellular, apicomplexan parasite that causes toxoplasmosis. Although the global prevalence of toxoplasmosis has been estimated to be approximately 30%, there is limited seroprevalence data in Ghana, with a dearth of information on the impact of T. gondii on haematological parameters in exposed perso...
Article
Full-text available
Background: We aimed at investigating the impact of malaria on the haematological parameters of residents from different demographic settlements in the Ashanti Region of Ghana. Malaria parasites trigger changes in certain haematological parameters, which may result in a number of clinical manifestations. Differences in demographic settlements, such...
Article
Full-text available
The study measured and compared quality parameters such as pH, moisture content, ash content and hydrogen peroxide concentration of three types of honey from different localities in Ghana. Using the agar well diffusion, antimicrobial potential of natural honey from two different sources as well as an adulterated honey were determined against Staphy...
Preprint
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Background: COVID-19 continues to wreak havoc in different countries across the world, claiming thousands of lives, increasing morbidity and disrupting lifestyles. The global scientific community is in urgent need of relevant evidence, to understand the challenges and knowledge gaps, as well as the opportunities to contain the spread of the virus....
Article
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Background: COVID-19 continues to wreak havoc in different countries across the world, claiming thousands of lives, increasing morbidity and disrupting lifestyles. The global scientific community is in urgent need of relevant evidence, to understand the challenges and knowledge gaps, as well as the opportunities to contain the spread of the virus....
Article
Background:Toxoplasma gondii is an obligate, intracellular, apicomplexan parasite that causes toxoplasmosis. Although the global prevalence of toxoplasmosis has been estimated to be approximately 30%, there is limited seroprevalence data in Ghana, with a dearth of information on the impact of T. gondii on haematological parameters in exposed person...
Article
Full-text available
Background: This study aimed at investigating haematological changes in malaria patients across different demographic settlements. Malaria parasites trigger changes in certain haematological parameters, which may result in a number of clinical manifestations. Differences in demographic settlements, such as rural, peri-urban and urban settlements, m...
Article
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Background As malaria transmission intensity declines, the heterogeneity in infectious burden becomes pronounced. There is thus the need for more sensitive tools to identify micro-geographic areas of higher risk for targeted interventions. We sought to evaluate several immunogenic peptides of P. falciparum, secreted ookinete and sporozoite proteins...
Article
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Background Over recent years, there has been an increase in the use of a histidine-rich protein 2(HRP-2)-based rapid diagnostic test (RDT) in the diagnosis of malaria. Accurate and prompt diagnosis of malaria will help reduce parasite reservoir and reduce malaria transmission. However, the underdiagnosis of malaria due to low parasite density hinde...
Article
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ABSTRACT Background: Intestinal parasites, especially coccidian parasites, cause gastrointestinal symptoms such as severe diarrhoea which increases morbidity and mortality rates in people living with Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS),particularly in Sub-Saharan Africa. We examined the prevalence of intestina...
Article
Onchocerciasis is one of the neglected tropical diseases caused by Onchocerca volvulus. Ivermectin is known to be effective in the treatment of onchocerciasis because it suppresses the production of microfilariae by the adult female worms for a few months following treatment thus reducing transmission. In this study, a deterministic model is develo...
Article
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Background: Scarce studies have addressed hematological differences of malaria in urban and rural regions. Methods: Full or complete blood cell counts from 46 and 75 individuals (age range from < 1 to 92 years) with uncomplicated malaria infection living in urban (Accra) and rural (Dodowa) Ghana, respectively, were assessed. Sickle cell trait and p...
Article
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Ebola virus is a severe, frequently fatal illness, with a case fatality rate up to 90%. The outbreak of the disease has been acknowledged by World Health Organization as Public Health Emergency of International Concern. The threat of Ebola in West Africa is still a major setback to the socioeconomic development. Optimal control theory is applied to...
Article
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Plasmodium falciparum infection can cause microvascular dysfunction, cerebral encephalopathy and death if untreated. We have previously shown that high concentrations of free heme, and C-X-C motif chemokine 10 (CXCL10) in sera of malaria patients induce apoptosis in microvascular endothelial and neuronal cells contributing to vascular dysfunction,...
Article
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Plasmodium falciparum infection can cause microvascular dysfunction, cerebral encephalopathy and death if untreated. We have previously shown that high concentrations of free heme, and C-X-C motif chemokine 10 (CXCL10) in sera of malaria patients induce apoptosis in microvascular endothelial and neuronal cells contributing to vascular dysfunction,...
Article
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Seroepidemiology provides robust estimates for tracking malaria transmission when intensity is low and useful when there is no baseline entomological data. Serological evidence of exposure to malaria vectors and parasite contribute to our understanding of the risk pathogen transmission, and facilitates implementation of targeted interventions. Ab t...
Article
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Malaria and HIV/AIDS are the two most common infections in sub-Sahara Africa. There are hypotheses and study reports on the possible association between these two infections, hence the prevalence and outcome of their co-infection in an endemic population will be important in defining healthcare strategies. A cross sectional study was carried out at...
Article
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Following studies on the transmission of Onchocerca volvulus (Leuckart) by Simulium sanctipauli Vajime & Dunbar (Diptera, Simuliidae) in Upper Denkyira District in Ghana in 2001 and 2002 (Kutin et al., Med Vet Ent 18:167-173, 2004), further assessments were carried out in 2006 and 2013/2014 to determine whether transmission parameters had changed s...
Conference Paper
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Assessments of the transmission of Onchocerca volvulus by Simulium sanctipauli in the Upper Denkyira District, Ghana, and the disappearance of the vector R. Garms1, K. Badu2, E. Owusu Dabo3, S. Baffour-Awuah3, 7, O. Adjei4, A.Y. Debrah3, 5, M. Nagel3, N.K. Biritwum6, L. Gankpala8, R.J. Post9 and T.F. Kruppa1, 3 1Bernhard Nocht Institute for Tro...
Article
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Malaria represents a major public health problem in Africa. In the East African highlands, the high-altitude areas were previously considered too cold to support vector population and parasite transmission, rendering the region particularly prone to epidemic malaria due to the lack of protective immunity of the population. Since the 1980's, frequen...
Conference Paper
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Human biting rates and mosquito infection rates vary across space and time. Mosquito populations also vary temporally, forced by environmental variables such as rainfall, temperature, and humidity. These sources of heterogeneity in the distribution of mosquito populations generate variability in the risk of human infection. Assessment of exposure t...
Article
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Background. Malaria transmission is heterogeneous. Villages close to each other may have very different transmission characteristics. The presence and abundance of malaria vectors is governed by local ecology and microclimate. Knowledge of the dynamics of transmission is important for planning and evaluation of malaria control strategies. This stud...
Article
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Abstract: Background: Changes in temperature, rainfall and relative humidity due to climate change are expected to directly influence malaria transmission dynamics, by modifying the behavior and geographical distribution of malaria vectors while shortening the sporogonic cycle of the parasite in the vector. These changes will have varying effects...
Article
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Background: The existing metrics of malaria transmission are limited in sensitivity under low transmission intensity. Robust surveillance systems are needed as interventions to monitor reduced transmission and prevention of rapid reintroduction. Serological tools based on antibody responses to parasite and vector antigens are potential tools for t...
Article
Full-text available
Following claims that some plants have antimicrobial activities against infectious microbes, the in vitro antimicrobial activities of different solvent fractions of ethanolic extract of Cryptolepis sanguinolenta were evaluated against eight standard bacteria and clinical isolates. The solvent partitioning protocol involving ethanol, petroleum ether...
Article
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Assessment of malaria endemicity at different altitudes and transmission intensities, in the era of dwindling vector densities in the highlands, will provide valuable information for malaria control and surveillance. Measurement of serum anti-malarial antibodies is a useful marker of malaria exposure that indicates long-term transmission potential....
Article
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Entomological Survey of Malaria Vectors within the Kumasi Metropolitan Area—A Study of Three Communities: Emena, Atonsu and Akropong Abstract: This study describes the bio-ecology of local Anopheles vectors, their abundance, type and vectoral competence in three communities around Kumasi Metropolis. The study sites were selected based on bio-ecolo...
Article
Full-text available
Background: The existing metrics of malaria transmission are limited in sensitivity under low transmission intensity. Robust surveillance systems are needed as interventions to monitor reduced transmission and prevention of rapid reintroduction. Serological tools based on antibody responses to parasite and vector antigens are potential tools for tr...
Conference Paper
Full-text available
MICRO-GEOGRAPHIC HETEROGENEITIES IN EXPOSURE TO ANOPHELES GAMBIAE SALIVARY GLAND PROTEIN IN DIFFERENT MALARIA ENDEMICITIES IN THE WESTERN KENYA HIGHLANDS Kingsley Badu1, Joram Siangla2, Franck Remoue3, John Ong’echa4, Guofa Zhou5, Andrew K. Githeko6, Guiyun Yan5 1Kenya Medical Research Institute, Kisumu, Kenya, 2Walter Reed Project, United States A...
Article
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Anopheles gambiae is the predominant malaria vector species in Ghana, western Africa, with a strong local presence of Anopheles melas Theobald along the southern coast. We studied the biting behavior of these two species of the Anopheles gambiae complex inland and at the coast in Ghana, with special attention to the local peoples' preference for ou...