Khwannarin Khemsom- Master of Science
- Research Associate at The Ohio State University
Khwannarin Khemsom
- Master of Science
- Research Associate at The Ohio State University
About
5
Publications
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Introduction
Skills and Expertise
Current institution
Publications
Publications (5)
Viruses with single-stranded, positive-sense (+) RNA genomes incur high numbers of errors during replication, thereby creating diversified genome populations from which new, better adapted viral variants can emerge. However, a definitive error rate is known for a relatively few (+) RNA plant viruses, due to challenges to account for perturbations c...
Viruses with single-stranded, positive-sense (+) RNA genomes incur high numbers of errors during replication, thereby creating diversified genome populations from which new, better adapted viral variants can emerge. However, a definitive error rate is known for a relatively few (+) RNA plant viruses, due to challenges to account for perturbations c...
During the translation process, transfer RNA (tRNA) carries amino acids to ribosomes for protein synthesis. Each codon of mRNA is recognized by a specific tRNA, and enzyme-catalysed modifications to tRNA regulate translation. TtcA is a unique tRNA-thiolating enzyme that requires an iron-sulfur ([Fe-S]) cluster to catalyse thiolation of tRNA. In thi...
Questions
Question (1)
I'm a microscopy assistant working in core facilities. I'm going to receive the nerve samples for TEM. The researcher perfused mice with 4% PFA and conducted immunohistochemistry labeling by using DAB staining. Then postfix with 2% PFA/2.5% glutaraldehyde in PBS for 3 hours. Wash the samples 3 times with PBS. The samples in PBS will arrive on Friday and process next week.
I'm curious if it is ok to leave the DAB stained samples in PBS at 4°C for 3 days? I will continue postfix with 2% OsO4 on Tuesday. How long can we leave the sample in PBS? Is the PBS going to affect the DAB stained over time? Any method/protocol suggested would be appreciated.
Best,
Khwannarin
