Khalid Y. Al-sharafa

Mu’tah University · Biology department

The beneficial application of silver nanoparticles and biostimulants to increase crop yield and quality is a long-term strategy to achieve desired agricultural productions that are resilient to various biotic and abiotic challenges. This project aimed to evaluate the individual effects of silver nanoparticles (AgNPs), Ascophyllum nodosum (SEW), and...

The study investigated prolonged drought stress effects on phytohormone and polyamine levels in cress leaves, elucidating plant responses to harsh environments. The study found that various phytohormones, including 12-oxophytodienoic acid (OPDA), Jasmonic acid (JA), Jasmonyl-Isoleucine (JA-Ile), Abscisic acid (ABA), and salicylic acid (SA), increas...

Efficacy of Sterculia diversifolia Leaf Extracts: Volatile Compounds, Antioxidant and Anti-Inflammatory Activity, and Green Synthesis of Potential Antibacterial Silver Nanoparticles

The plants, Althaea officinalis, Tilia cordata and Psidium guaja have been used traditionally to treat respiratory infection symptoms. Flowers of A. officinalis and leaves of T. cordata and P. guaja have been used to treat cough, sore throat, catarrh, oral and pharyngeal mucosa irritation. Therefore, this study was designed to examine the antibacte...

Juniperus phoenicea from five natural populations in southern Jordan were collected and their genetic variations using inter simple sequence repeats (ISSR) were studied. The hydro-distilled essential oils (EOs) from three geographical regions were characterized by their chemical composition and antibacterial activity. ISSR data analysis for the two...

In this study, the regulation of ascorbate peroxidase (APX) specific activity, anthocyanin, carotenoid, hydrogen peroxide, lipid peroxidation, and protein levels in cress leaves in response to different abiotic stresses were investigated. The total APX specific activity was significantly elevated after 9 days of drought treatment, short-term (2 h)...

Plant growth regulators (PGRs) were involved in several types of abiotic stress responses by means of improving seed germination and modifying the growth and development of medicinally important Lepidium sativum via alleviating the negative effects of abiotic stresses. Therefore, the present research was carried out to investigate the effects of ex...

Abstract: Brevibacterium spp.that was already isolated from wastewater treatment plant of Petra City, Jordan. While 1 and 2 mM of 3,4-dichloro benzoic acid (3,4-DCBA) were used, the biodegradation rate was 14.5 and 21.25 µM/h, respectively, and the remaining was 58% for both. The remaining ratio to the quantity of 3,4-DCBA used was taken in conside...

Brevibacterium spp.that was already isolated from wastewater treatment plant of Petra City, Jordan. While 1 and 2 mM of 3,4-dichloro benzoic acid (3,4-DCBA) were used, the biodegradation rate was 14.5 and 21.25 µM/h, respectively, and the remaining was 58% for both. The remaining ratio to the quantity of 3,4-DCBA used was taken in consideration, as...

Centaurea damascena a member of Asteraceae family was investigated for its aerial part essential oil composition and antibacterial activities. A total of 35 Components were characterized with the presence of oxygenated terpenes that represents more than 86 % of the total essential oil components. The composition was dominated by 11.45 % fokienol, 8...

Centaurea damascena a member of Asteraceae family was investigated for its aerial part essential oil composition and antibacterial activities. A total of 35 Components were characterized with the presence of oxygenated terpenes that represents more than 86 % of the total essential oil components. The composition was dominated by 11.45 % fokienol, 8...

Purpose: To carry out eco-friendly biosynthesis of fungi-derived silver nanoparticles (AgNPs) and investigate their antibacterial synergies with essential oils (EOs) of Asteriscus graveolens (Forssk.) Less. and Cupressus sempervirens. Methods: Biosynthesis of AgNPs was carried out using a cell-free filtrate of Tritirachium oryzae. The biosynthesize...

It is worth mentioning that the high output of different physiological responses under the expression of vgb, may have a considerable effect on the enzyme productivity, dairy industry, heavy metal uptake, biodegradation of different organic pollutants and other applications. The expression of bacterial haemoglobin is useful in lessening the load of...

Description A major focus of nanotechnology concerns is the expansion of the optimization of nanomaterials in purity, size and dispersity. Method In the current work, a two-step AgNP synthesis process was optimized at the mycelia-DI water suspension and AgNP formation reaction levels. Results Biomass filtrate from the fungal strain Tritirachium o...

Centaurea damascena a member of Asteraceae family was investigated for its aerial part essential oil composition and antibacterial activities. A total of 35 components were characterized with the presence of oxygenated terpenes that represents more than 86 % of the total essential oil components. The composition was dominated by 11.45 % fokienol, 8...

The substrate range of 2,4-dinitrotoluene (DNT) dioxygenase was investigated by measuring substrate-dependent O2 uptake and maximum growth (expressed in A600) on substrate-containing minimal medium. The control for each strain had no added particular substrate. The following aromatic compounds: catechol, α-naphthalene acetic acid, β-dimethylaminobe...

The extracellular lipase produced by Acinetobacter sp. K5b4 was purified to homogeneity using ultrafiltration (cutoff 30 KDa) followed by gel filtration chromatography on Sephadex G-50. The enzyme was purified to homogeneity with an apparent molecular mass of 133 KDa by SDS-PAGE. This purification resulted on 10.24 fold with 18.3% recovery. The Km...

Crop yield and quality are limited by different biotic and abiotic factors. This study focused on the ability of phenylalanine ammonia-lyase, ascorbate peroxidase and polyphenol oxidase to impart resistance to mineral deficiencies. Tomato plants (Lycopersicon esculentum L.) were grown for 48, 72 and 96 hr with different specific deficiencies of min...

Alkhsabah et al.: Effects of abiotic factors on internal homeostasis of Mentha spicata leaves-2537-APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH 16(3):2537-2564. Abstract. Mentha spicata plants were subjected to various abiotic stresses in order to evaluate the triggering of signals and the phenolic compound levels of leaves. Total phenolic compound l...

The study examined photosynthetic efficiency of two barley landraces (cvs. Arabi Abiad and Arabi Aswad) through a prompt fluorescence technique under influence of 14 different abiotic stress factors. The difference in the behavior of photosynthetic parameters under the same stress factor in–between cv. Arabi Abiad and cv. Arabi Aswad indicated diff...

Background and objective: The patients with Diabetes Mellitus (DM) have malfunction in bladder which prompt urine accumulation in its pool which serves a decent situation to the microbes to be develop and cause Urinary Tract Infection (UTI). The UTI is the most infectious disease that affects both males and females. This study was designed to dete...

The detection of hydrogen peroxide, chlorophyll pigments, anthocynin, carotenoids, total phenolic compounds and lipid peroxidation levels as potential stress signaling molecules in tomato (Lycopersicon esculentum Mill.) leaves in response to specific mineral deficiency were studied. The stress signaling molecules were measured in the plant leaves a...

Very little has been published on the essential oil composition of Ruta graveolens worldwide. Herein, we report on the essential oil composition of Ruta graveolens growing in Jordan. The essential oil was isolated using hydrodistillation from the aerial parts of Ruta graveolens L and a chemical composition analysis was conducted using Gas Chromatog...

Endophytic fungus isolated from Pistacia atlantica Desf. which associated with leaves necrotic spotting was identified using nuclear ribosomal DNA internal transcribed spacer regions (ITS1-5.8S rDNA-ITS2) as Ulocladium sp. VL204 a fungus member of Pleosporaceae family.

High light acclimation depends on retrograde control of nuclear gene expression. Retrograde regulation uses multiple signalling pathways and thus exploits signal patterns. To maximally challenge the acclimation system, Arabidopsis thaliana plants were either adapted to 8 (low light (L-light)) or 80 µmol quanta m(-2) s(-1) (normal light (N-light)) a...

Regulation of the expression of nuclear genes encoding chloroplast proteins allows for metabolic adjustment in response to changing environmental conditions. This regulation is linked to retrograde signals that transmit information on the metabolic state of the chloroplast to the nucleus. Transcripts of several APETALA2/ETHYLENE RESPONSE FACTOR tra...

Chloroplasts are equipped with a nuclear-encoded antioxidant defence system the components of which are usually expressed at high transcript and activity levels. To significantly challenge the chloroplast antioxidant system, Arabidopsis thaliana plants, acclimated to extremely low light slightly above the light compensation point or to normal growt...

A successful attempt was made to isolate linear alkylbenzene sulfonate (LAS)-degrading bacteria from soil irrigated with wastewater. The isolated bacteria were able to use LAS as sole carbon and energy source. Maximum growth rates on LAS reached only 0.27 h(-1). 16S-rRNA sequencing and fatty-acid analysis placed the bacteria in the genus Enterobact...

Using the two plasmids, pUC8:16 and pUC9 in the cell grown on different carbon and nitrogen sources afforded comparable levels of copper uptake by Escherichia coli . Previously, no such investigations have been made, particularly using bacterial hemoglobin (VHb), carbon and nitrogen sources. It is clear from these results that the type of either ca...

At fixed concentration (0.5%), lactose and galactose acted as inducers while glucose and other tested carbon sugars showed repression effects on β-galactosidase production in Enterobacter aerogenes strain. The expression of Vitreoscilla hemoglobin gene (vgb) in this bacterial strain managed to overcome the repression effects as well as improving th...