Khaled Khleifat

• Professor of Microbiology, Al-Ahliyya Amman University
• Professor (Full) at Mutah University

The effect of transnitrosation intermediate between S-nitroso-N-acetylcysteine (NACysNO) and cysteine on the growth of vgb-bearing Enterobacter aerogenes was investigated using three parameters: the ratio of the specific growth rates, the inhibition zone, and alpha-amylase synthesis for the culture exposed to stressors to that of the same stressor-...

Escherichia coli alphaDH5 harboring Vitreoscilla hemoglobin gene, vgb (Escherichia:pUC8:16), and control strains (parental and vgb-lacking transformed strains, E. coli:pUC9) were employed for studying the potential ability of copper uptake. The strains were grown on a minimal medium, 5XM9, that contained different carbon (mannitol, fructose, lactos...

To evaluate the effects of bacterial haemoglobin on bacterial growth and alpha-amylase formation under different aeration conditions. Enterobacter aerogenes was transformed with the gene encoding Vitreoscilla (bacterial) haemoglobin, vgb. The growth kinetics and ability to synthesize alpha-amylase enzyme were investigated in this transformed Entero...

Pseudomonas aeruginosa was isolated from infected burn patients and characterized by standard biochemical tests. The in vitro copper uptake was compared between this isolated pathogenic strain and two non-pathogenic control strains of Gram positive bacteria Bacillus thuringiensis strain Israelis as well as Gram negative bacteria Enterobacter aeroge...

Ewingella americana was grown in batch cultures using a phenol-containing M9 minimal medium. Phenol was found to be the sole source for carbon and energy. Phenol was found to inhibit the growth rate with a maximum concentration of 1100 ppm, beyond which no growth occurred. The Haldane model was used to predict the specific growth rate-concentration...

In the original publication [...]

Silver nanoparticles (AgNPs) are spherical particles with a number of specific and unique physical (such as surface plasmon resonance, high electrical conductivity and thermal stability) as well as chemical (including antimicrobial activity, catalytic efficiency and the ability to form conjugates with biomolecules) properties. These properties allo...

Silver nanoparticles (AgNPs) are spherical particles with a number of specific and unique physical (such as surface plasmon resonance, high electrical conductivity and thermal stability) as well as chemical (including antimicrobial activity, catalytic efficiency and the ability to form conjugates with biomolecules) properties. These properties allo...

This study aimed to evaluate the effectiveness of Cynomorium coccineum extract on several quorum sensing dependent mechanisms, including biofilm formation, the production of virulence factors, and the production of autoinducers in Pseudomonas aeruginosa. The impact on biofilm formation has been evaluated using a crystal violet assay and visualized...

Introduction Given the increasing frequency of drug-resistant bacteria and the limited progress in developing new antibiotics, it is necessary to explore new methods of combating microbial infections. Nanoparticles, particularly silver nanoparticles (Ag-NPs), have shown exceptional antibacterial characteristics; however, elevated concentrations of...

Background: Red blood cells go through a number of metabolic changes throughout the course of a 0-35 day storage period at 1 to 6oC. The sodium/potassium pump is immobilized, resulting in a drop in intracellular potassium with an increase in cytoplasmic sodium levels, a fall in glucose levels, acidosis as a result of low pH levels, and other altera...

Numerous marine sponge bioactive compounds exhibit diverse pharmacological properties. This study investigated the ethyl acetate extract of Amphimedon chloros for its effects on breast cancer cells (MDA-MB-231), focusing on the expression of key genes involved in apoptosis and cell cycle regulation, including Bax, Cyclin D, caspase 3, p21, C-myc, a...

This study intends to investigate the potential use of mmBCFA, which includes 10 MTD, 12 MTD, 13 MTD, and 14 MTD, as a treatment option for cancer. This is due to the close correlation between metabolic dysregulation and the mmBCFA presence. We looked at how mmBCFA affected several cancer cell lines, such as those from the pancreas, colon, and brea...

Healing Burn wound Antioxidant a b s t r a c t Anchusa azurea one of the medicinal plants that has been traditionally used for treat burn wounds. However, the traditional claim that A.azurea can hasten burn wound healing has not been supported by scientific studies. This experiment used a male Wistar rats model to investigate the activity of A. azu...

Background: Both vitamin D and Fe micronutrient deficiencies are common in Jordan, but the association between them is unclear. Iron and vitamin D deficiencies are common disorders that cause a variety of health issues in the population. Objectives: The present study sought to elucidate the potential association between Vitamin D status and iron de...

Hashimoto’s thyroiditis (HT) is an autoimmune disorder characterized by elevated thyroid-stimulating hormone (TSH) levels. This research investigates the complex interaction between HT and cardiovascular risk in adult Jordanian non-pregnant women aged 20-50. Through a study involving 50 HT subjects and 40 healthy subjects, the levels of lipoprotein...

This study intends to investigate the potential use of mmBCFA, which includes 10 MTD, 12 MTD, 13 MTD, and 14 MTD, as a treatment option for cancer. This is due to the close correlation between metabolic dysregulation and the mmBCFA presence. We looked at how mmBCFA affected several cancer cell lines, such as those from the pancreas, colon, and brea...

Anchusa azurea one of the medicinal plants that has been traditionally used for treat burn wounds. However, the traditional claim that A.azurea can hasten burn wound healing has not been supported by scientific studies. This experiment used a male Wistar rats model to investigate the activity of A. azurea aerial parts methanolic extract in burn wou...

The emergence of drug-resistant microorganisms presents a substantial global public health threat. The increase in pathogens resistant to commonly prescribed antibiotics underscores the urgent requirement to explore alternative treatment strategies. This study adopts a novel approach by harnessing natural resources, specifically essential oils (EO)...

Introduction: Medicinal plants are considered an important source of human health because of their therapeutic capabilities in treating various diseases. This study aimed to evaluate the phytochemical profile, antioxidant, and cytotoxic activities of the methanolic extracts of three wild medicinal plants (Ruta graveolens, Peganum harmala and Citrul...

Alkaptonuria is a rare genetic disease caused by defect in the phenylalanine and tyrosine metabolism due to a deficiency in homogentisate 1,2-dioxygenase enzyme. The formation of ochronotic brownish-black pigment is the hallmark of the disease and at later stage AKU patients complain of recurrent renal calculi formation. In this report, we describe...

discusses the development of rutin‐loaded nanoliposomes and their anti‐colorectal cancer activity against human carcinoma cells (HT‐29). The study characterizes the nanoliposomes using the thin‐film hydration method and analyzes their size, charge, and polydispersity index. The encapsulation efficiency and drug loading ability of rutin at different...

Monosodium glutamate (MSG) is used in food-additives, and the Food and Drug Administration has placed it under intense scrutiny following several reports that it causes glutamate neurotoxicity. Ashwagandha (ASH) roots are traditionally used for memory enhancement. This study aimed to evaluate the nootropic activity of ASH as well as its therapeutic...

Chronic wounds have become a major concern for healthcare systems, as they have been related to diabetic foot ulcers, venous leg ulcers and pressure ulcers. Oleuropein is an active compound that is extracted from olive leaves and it has the ability to reduce injury to tissues owing to its antioxidant effect, hence improving wound healing. The poor...

Context: Echinococcus granulosus (E. granulosus) is a significant zoonotic agent in veterinary and medicinal fields. Surgery and benzimidazole drugs are used as treatments but have significant drawbacks. Therefore, there is growing interest in ethnomedical approaches to investigating the scolicidal properties of plants traditionally as anthelmintic...

Diabetes is one of the most common chronic diseases worldwide. This study aims to evaluate the hypoglycemic and hypolipidemic effects of Crataegus aronia (C. aronia) ethanolic extract and Crataegus aronia phytosome (C. aronia-p) in streptozotocin (STZ)-induced diabetic rats. The phytochemical content of C. aronia was identified. The C. aronia ethan...

The synthesis of reliable biological nanomaterials is a crucial area of study in nanotechnology. In this study, Emericella dentata was employed for the biosynthesis of AgNPs, which were then combined with synthesized biochar, a porous structure created through biomass pyrolysis. The synergistic effects of AgNPs and biochar were evaluated through th...

Abstract: The synthesis of reliable biological nanomaterials is a crucial area of study in nanotechnology. In this study, Emericella dentata was employed for the biosynthesis of AgNPs, which were then combined with synthesized biochar, a porous structure created through biomass pyrolysis. The synergistic effects of AgNPs and biochar were evaluated...

Background/Objective Alzheimer's disease (AD) is mainly characterized by amnesia that affects millions of people worldwide. This study aims to explore the effectiveness capacities of bee venom (BV) for the enhancement of the memory process in a rat model with amnesia-like AD. Methods The study protocol contains two successive phases, nootropic and...

This study aimed to explore the preventive effect of the methanolic extracts of the seeds of Citrullus colocynthis, and the aerial parts of Ruta graveolens and Peganum harmala on the development of secondary infection of Echinococcus granulosus in vivo. The viable protoscolices were subjected to 40 mg/mL of methanolic extracts of the three plants f...

This study aimed to see how effective Globulari. arabica and Malva. slyvestries-based cream formulations were at healing scald burn wounds in rats. Depending on ointment preparations of 1%, 5%, and 10% w/w were created. For comparison, an ointment base and a regular burn cream composed soframycine were utilized. Rats introduced a burn by solidifyin...

Silver nanoparticles (AgNPs) have been shown to be able to inhibit bacterial growth while presenting a negligible threat to the development of bacterial resistance and the toxicity of conventional silver compounds to human cells. Various concentrations of R. graveolens MetOH extract, both by itself and in combination with AgNPs made by Aspergillus...

Alzheimer's disease is regarded as a common neurodegenerative disease that may lead to dementia and the loss of memory. We report here the nootropic and anti-amnesic effects of both peppermint and rosemary oils using a rat model of scopolamine-induced amnesia-like AD. Rats were administered orally with two doses (50 and 100 mg/kg) of each single oi...

Background: Silver nanoparticles (AgNPs) are attractive substrates for new medicinal treatments. Biochar is pyrolyzed biomass. Its porous architecture allows it to hold and gather minuscule particles, through which nanoparticles can accumulate in its porous structure. This study examined AgNPs’ antibacterial and anticancer properties alone and comb...

Medicinal plants represent an essential source of active biological compounds. The current study examined the methanol extract of V. iphionoides for its antibacterial, antioxidant, and antiinflammatory activities. The antibacterial activity was evaluated against six bacterial strains (Staphylococcus xyloses, Escherichia coli, Klebsiella oxytoca, En...

Background: The aim of the current study is to determine whether there is an association between alkaptonuria (AKU) and urinary tract infection (UTI) by exploring the bacterial quality of the urinary tract, as most of the patients with AKU present with frequent occurrence of UT symptoms such as incomplete emptying of urinary bladder, dysuria and n...

Background: This study sought to determine whether (1) evidence is available of interactions between anodal tDCS and oscillated tDCS stimulation patterns to increase the power of endogenous brain oscillations and (2) the frequency matching the applied anodal otDCS's frequency and the brain's dominant intrinsic frequency influence power shifting du...

Background: This study sought to determine whether (1) evidence is available of interactions between anodal tDCS and oscillated tDCS stimulation patterns to increase the power of endogenous brain oscillations and (2) the frequency matching the applied anodal otDCS's frequency and the brain's dominant intrinsic frequency influence power shifting dur...

Resistance to antibiotics is a significant public health issue in preventing infectious diseases. This study was designed to evaluate the antibacterial activity of eucalyptol, γ-terpinene, p-cymol and punicalagin against Methicillin (Oxacillin) resistant strains. The synergistic effect of these compounds with cefotaxime against Staphylococcus aureu...

Sex hormones have a large influence on the structure and function of vaginal microbiota. A delicate balance between the vaginal microbiota and the host is one of the most important health reasons for women. The purpose of this study was to see if there were any links between female sex hormone levels and vaginal microbial diversity. A total of 100...

Phenol is a major worry pollutant resulting from industrialized manufacturing and chemical reactions. The growth kinetics and biodegradation of phenol were initially investigated using C. flaccumfaciens, a recently identified plant growth stimulating bacterium. Based on the Haldane inhibition model, Haldane's growth kinetics inhibition coefficient...

Background and Objectives: Nanoscience is one of the most important branches of modern science, which deals with the knowledge, structure, and properties of nanoparticles. This study aimed to investigate the ability of an airborne fungus (Aspergillus flavus) to synthesize silver nanoparticles (AgNPs) and to test the antibacterial activity of the sy...

Purpose: The current study aimed to explore the in vivo therapeutic effects of the methanolic extracts of Citrullus colocynthis, Ruta graveolens, and Peganum harmala against hydatid cyst secondary infection. Methods: Aerial parts of P. harmala and R. graveolens, including leaves and stems, and seeds of C. colocynthis were collected and extracted...

Background One of the primary health concerns for diabetes individuals is wounds. The used drugs have several side effects, urging the need for new natural sources for therapeutics. Materials and methods This study was designed to estimate the wound healing potential of the methanolic extract of Globularia arabica and Malva sylvestris leaves and R...

Microbial antibiotic resistance is rapidly increasing as a result of overuse or misuse of antibiotics, as well as a lack of new, effective antibiotics. Alternative antimicrobial treatments, such as nanoparticles, and their potential for stronger synergetic effect when paired with other active chemicals, could be a viable option. This study is prepa...

Introduction: A cost-effective and ecologically friendly method of generating silver nanoparticles (AgNPs) includes pathways that utilize a variety of biological sources to decrease metal ions. This study was designed to synthesize AgNPs using a fungus strain Aspergillus flavus and evaluate its antibacterial activities alone or in combination with...

One of the most important roles for nanotechnology concerns is the development of optimizable experimental protocols for nanomaterials synthesis. The formation of silver nanoparticles (AgNPs) was supported by Rhizopus stolonifer MR11, which was isolated from olive oil mill soil samples. The ability of R. stolonifer MR11 to biosynthesize silver nano...

Background: Ephedra foeminea is known in Jordan as Alanda and traditionally. It is used to treat respiratory symptoms such as asthma and skin rashes as an infusion in boiling water. The purpose of this study was to determine the antidiabetic property of Ephedra foeminea aqueous extract in streptozotocin-induced diabetic rats. Methods: The aqueou...

Echinococcosis is a common and endemic disease that affects both humans and animals. In this study, the in vitro activities of methanolic extracts of Ruta graveolens, Peganum harmala aerial parts, and Citrullus colocynthis seeds against protoscolosis and isolated bacterial strains from hydatid cysts were assessed using disc diffusion methods and Mi...

Phenol is a severe pollutant that harms the environment and, potentially, human health. This study aimed to investigate the biodegradability of phenol by the plant growth-promoting bacterium R. nepotum. That included studying the growth kinetics and the effects of growth conditions such as incubation temperature, pH, and the use of different substr...

Echinococcosis is a common and endemic disease that affects both humans and animals. In this study, the in vitro activities of methanolic extracts of Ruta graveolens , Peganum harmala aerial parts, and Citrullus colocynthis seeds against protoscolosis and isolated bacterial strains from hydatid cysts were assessed using disc diffusion methos and Mi...

One of the main organic pollutants that could result from industrial products and chemical transformations is phenol. In the current study, the kinetics of Serratia odorifera, which was isolated from arable soil, was studied by growing it on broth minimal medium spiked with phenol as only carbon source and energy. The newly isolated plant growth-pr...

One of the main organic pollutants that could result from industrial products and chemical transformations is phenol. In the current study, the kinetics of Serratia odorifera, which was isolated from arable soil, was studied by growing it on broth minimal medium spiked with phenol as only carbon source and energy. The newly isolated plant growth-pr...

Introduction: Fungi are one of the main approaches for synthesis of metallic nanoparticles (NPs), which can have medical and biotechnological applications such as their role in anti-bacterial, anti-cancer and various industrial activities. Objective: The current research focused on the biosynthesis of silver nanoparticles (AgNPs) using airborne fun...

Phenol is one of the main pollutants that have a serious impact on the environment and can even be very critical to human health. The biodegradation of phenol can be considered an increasingly important pollution control process. In this study, the degradation of phenol by Bacillus simplex was investigated for the first time under different growth...

Phenol is one of the main pollutants that have a serious impact on the environment and can even be very critical to human health. The biodegradation of phenol can be considered an increasingly important pollution control process. In this study, the degradation of phenol by Bacillus simplex was investigated for the first time under different growth...

Phenol is one of the main pollutants that have a serious impact on the environment and can even be very critical to human health. The biodegradation of phenol can be considered an increasingly important pollution control process. In this study, the degradation of phenol by Pseudomonas fredriksbergsis was investigated for the first time under differ...

Phenol is one of the most important environmental pollutants that are found in sewage and industrial water from which agricultural lands are irrigated. In this study Curtobacterium flaccumfaciens bacterium was isolated in pure culture from phenol-uncontaminated farmland soil. Its ability to biodegrade phenol was examined. C. flaccumfaciens demonstr...

The Jordanian endemic medicinal plant, Cupressus sempervirens was obtained from Dhana Natural Reserve, Al-Tafilah, Jordan to investigate its antibacterial and antioxidant activities. The procuring of essential oil was made by processing of dry leaves of C. sempervirens using steam-distillation method giving 0.26% (w/w) yield. The analysis of obtain...

Background and aims: Branched chain amino acids (BCAAs) can be tightly connected to metabolism syndrome (MetS) which can be counted as a metabolic indicator in the case of insulin resistance (IR). The aim of this study was to assess the potential role of these acids under oxidative stress. Material and Methods: the in vitro antioxidant activity of...

The scolicidal agents used to inactivate the protoscoleces through the surgery of hydatid cyst still produce detrimental side effects. The aim of this study was to find out the in vitro scolicidal effects achievable by Ruta graveolens methanol extract against the hydatid cysts protoscoleces. The cytotoxic activity of this plant also was evaluated a...

The common scolicidal agents used for the immobilization of protoscolices of hydatid cyst are mostly associated with side effects. The purpose of the current study was to investigate the methanol extracts of Ruta graveolens, Peganum harmala L. and seeds of Citrullus colocynthis L effects on hydatid cysts. The protoscolices were collected from hydat...

The medical usage of honey has become popular as an alternative and complementary treatment in recent time. This study was aimed at determining the inhibitory effect of honey and propolis against various species of Gram-positive and Gram-negative bacteria. The antibacterial activity was assessed using the disc diffusion and macro-dilution methods....

Flavonoids and branched chain amino acids (BCAAs) have been recognized as attractive agents for research due to their role in diabetes and cancer. The present study investigates the antiglycation effect of BCAAs and flavonoid compounds and their cytotoxic effect on different colorectal cancer cell lines individually and in combination. This study w...

The plants, Althaea officinalis, Tilia cordata and Psidium guaja have been used traditionally to treat respiratory infection symptoms. Flowers of A. officinalis and leaves of T. cordata and P. guaja have been used to treat cough, sore throat, catarrh, oral and pharyngeal mucosa irritation. Therefore, this study was designed to examine the antibacte...

Juniperus phoenicea from five natural populations in southern Jordan were collected and their genetic variations using inter simple sequence repeats (ISSR) were studied. The hydro-distilled essential oils (EOs) from three geographical regions were characterized by their chemical composition and antibacterial activity. ISSR data analysis for the two...

In this study, the regulation of ascorbate peroxidase (APX) specific activity, anthocyanin, carotenoid, hydrogen peroxide, lipid peroxidation, and protein levels in cress leaves in response to different abiotic stresses were investigated. The total APX specific activity was significantly elevated after 9 days of drought treatment, short-term (2 h)...

Many medicinal herbs are widely used in traditional folk medicine in most countries of the world, including Jordan, to treat many human diseases including microbial infections, diabetes and cancer. This study was conducted to evaluate the anti-cancer and antibacterial effects of essential oil (EO) of Varthemia Iphionoides, as well as the synergisti...

Plant growth regulators (PGRs) were involved in several types of abiotic stress responses by means of improving seed germination and modifying the growth and development of medicinally important Lepidium sativum via alleviating the negative effects of abiotic stresses. Therefore, the present research was carried out to investigate the effects of ex...

Bacterium Aeromonas hydrophila (A. hydrophila) was isolated from the Petra Wastewater Treatment Plant effluent in southern Jordan. It was identified by using morphological and biochemical characteristics. A. hydrophila was found to be able of using chlorobenzoate compounds as carbon and energy source. These capabilities were with different biodegra...

Centaurea damascena a member of Asteraceae family was investigated for its aerial part essential oil composition and antibacterial activities. A total of 35 Components were characterized with the presence of oxygenated terpenes that represents more than 86 % of the total essential oil components. The composition was dominated by 11.45 % fokienol, 8...

The family Asteraceae include large number of Centaurea species which have been applied in folk medicine. One of the family Asteraceae members is the Centaurea damascena which authentically been tested for its antibacterial and antioxidant activity as well as its toxicity. The aims of the study were to determine the antimicrobial and antioxidant ac...

Centaurea damascena a member of Asteraceae family was investigated for its aerial part essential oil composition and antibacterial activities. A total of 35 Components were characterized with the presence of oxygenated terpenes that represents more than 86 % of the total essential oil components. The composition was dominated by 11.45 % fokienol, 8...

Purpose: To carry out eco-friendly biosynthesis of fungi-derived silver nanoparticles (AgNPs) and investigate their antibacterial synergies with essential oils (EOs) of Asteriscus graveolens (Forssk.) Less. and Cupressus sempervirens. Methods: Biosynthesis of AgNPs was carried out using a cell-free filtrate of Tritirachium oryzae. The biosynthesize...

The main object of this investigation was to shed light on the information on the level of Cu uptake by Pseudomonas aeruginosa that has been previously isolated from patients using Gram-negative E. aerogenes ans a Gram-positive Bacillus thuringiensis as a control for Cu uptake measurements. Cu uptake data showed that maximum Cu uptake was obtained...

The main object of this investigation was to shed light on the information on the level of Cu uptake by Pseudomonas aeruginosa that has been previously isolated from patients using Gram-negative E. aerogenes ans a Gram-positive Bacillus thuringiensis as a control for Cu uptake measurements. Cu uptake data showed that maximum Cu uptake was obtained...

Recently, there has been an increased attention towards the phenomena of climate change in the Arab countries, after experiencing historical and sudden changes in weather (Lonergan, and Kavanagh, 1991). Some of the Arab countries have launched action plans to reduce the effects of desertification (UNEP, 1997). Intergovernmental Panel on Climate Cha...

The microbial community of semi-arid bare soil of Al-Ghweir Agricultural Research Station (GARS) from Jordan was identified and classified for their plant growth functions. This work is a part of an international research project under the frame of ERANETMED called (SUPREME). It aimed to find out a suitable mitigation measure for confronting the cl...

Antibactrial activity of Asteriscus graveolens methanolic extract and its synergy effect with fungal mediated silver nanoparticles (AgNPs) against some enteric bacterial human pathogen was conducted. Silver nanoparticles were synthesized by the fungal strain namely Tritirachium oryzae W5H as reported early. In this study, MICs of AgNPs against E. a...

The family Asteraceae include large number of Centaurea species which have been applied in folk medicine. One of the family Asteraceae members is the Centaurea damascena which authentically been tested for its antibacterial and antioxidant activity as well as its toxicity. The aims of the study were to determine the antimicrobial and antioxidant ac...

It is worth mentioning that the high output of different physiological responses under the expression of vgb, may have a considerable effect on the enzyme productivity, dairy industry, heavy metal uptake, biodegradation of different organic pollutants and other applications. The expression of bacterial haemoglobin is useful in lessening the load of...

Antibactrial activity of Asteriscus graveolens methanolic extract and its synergy effect with fungal mediated silver nanoparticles (AgNPs) against some enteric bacterial human pathogen was conducted. Silver nanoparticles were synthesized by the fungal strain namely Tritirachium oryzae W5H as reported early. In this study, MICs of AgNPs against E. a...

The family Asteraceae include large number of Centaurea species which have been applied in folk medicine. One of the family Asteraceae members is the Centaurea damascena which authentically been tested for its antibacterial and antioxidant activity as well as its toxicity. The aims of the study were to determine the antimicrobial and antioxidant ac...

It is worth mentioning that the high output of different physiological responses under the expression of vgb, may have a considerable effect on the enzyme productivity, dairy industry, heavy metal uptake, biodegradation of different organic pollutants and other applications. The expression of bacterial haemoglobin is useful in lessening the load of...