Kevin Wamae

Kevin Wamae
University of North Carolina at Chapel Hill | UNC · Institute for Global Health and Infectious Diseases (School of Medicine)

Doctor of Philosophy

About

33
Publications
2,413
Reads
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94
Citations
Citations since 2016
33 Research Items
94 Citations
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2016201720182019202020212022010203040
Additional affiliations
January 2021 - present
University of North Carolina at Chapel Hill
Position
  • PostDoc Position
November 2019 - October 2020
KEMRI-Wellcome Trust Research Programme
Position
  • PostDoc Position
Description
  • Malaria is a major disease burden in sub-Saharan Africa, resulting in millions of clinical episodes and thousands of deaths annually. I work on characterizing the genetic epidemiology of Plasmodium falciparum (the malaria-causing parasite), to determine what impact malaria interventions have on the parasite, such as the development of antimalarial drug-resistance. Such information help to tailor-make interventions that are effective against malaria.
Education
November 2015 - October 2019
The Open University (UK)
Field of study
  • Molecular Biology and Bioinformatics
September 2011 - December 2014
University of Nairobi
Field of study
  • Biotechnology and Bioinformatics
January 2007 - December 2010
Makerere University
Field of study
  • Biomedical Sciences

Publications

Publications (33)
Article
Full-text available
Background High levels of genetic diversity are common characteristics of Plasmodium falciparum parasite populations in high malaria transmission regions. There has been a decline in malaria transmission intensity over 12 years of surveillance in the community in Kilifi, Kenya. This study sought to investigate whether there was a corresponding redu...
Article
Full-text available
Introduction Genotyping Plasmodium falciparum sub-populations in malaria infections is an important aspect of malaria molecular epidemiology to understand within-host diversity and the frequency of drug resistance markers. Methods We characterised P. falciparum genetic diversity in asymptomatic infections and subsequent first-febrile infections us...
Article
Full-text available
Antimalarial therapeutic efficacy studies are routinely conducted in malaria-endemic countries to assess the effectiveness of antimalarial treatment strategies. Targeted amplicon deep sequencing (TADS) uniquely identifies and quantifies genetically distinct parasites within an infection. In this study, TADS Plasmodium falciparum apical membrane ant...
Article
Full-text available
Molecular surveillance of Plasmodium falciparum parasites is important to track emerging and new mutations and trends in established mutations and should serve as an early warning system for antimalarial resistance. Dried blood spots were obtained from a Plasmodium falciparum malaria survey in school children conducted across eight counties in west...
Article
Full-text available
Background: The COVID-19 pandemic relies on real-time polymerase chain reaction (qRT-PCR) for the detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), to facilitate roll-out of patient care and infection control measures. There are several qRT-PCR assays with little evidence on their comparability. We report alterations to the...
Article
Full-text available
Background: There are limited studies in Africa describing the epidemiology, clinical characteristics and serostatus of individuals tested for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We tested routine samples from the Coastal part of Kenya between 17 th March 2020 and 30 th June 2021. Methods: SARS-CoV-2 infections i...
Article
Full-text available
Background The invasion of the red blood cells by Plasmodium falciparum merozoites involves the interplay of several proteins that are also targets for vaccine development. The proteins Pf Rh5- Pf Ripr- Pf CyRPA- Pf p113 assemble into a complex at the apical end of the merozoite and are together essential for erythrocyte invasion. They have also be...
Article
Full-text available
Artemisinin resistance (AR) emerged in South East Asia 13 years ago and the identification of the resistance conferring molecular marker, Plasmodium falciparum Kelch 13 (Pfk13), 7 years ago has provided an invaluable tool for monitoring AR in malaria endemic countries. Molecular Pfk13 surveillance revealed the resistance foci in the Greater Mekong...
Article
Full-text available
Busseola fusca (Fuller) (Lepidoptera: Noctuidae), the maize stalk borer, is a widespread crop pest in sub-Saharan Africa that has been the focus of biological research and intensive management strategies. Here, we present a comprehensive annotated transcriptome of B. fusca (originally collected in the Western Province of Kenya) based on ten pooled...
Article
Full-text available
Background: The global COVID-19 outbreak relies on a quantitative real-time polymerase chain reaction (qRT-PCR) for the detection of severe acute respiratory syndrome coronavirus (SARS-CoV-2), to facilitate the roll-out of patient care and infection control measures. There are several qRT-PCR assays with little evidence on their comparability. We r...
Article
Full-text available
Malaria is still a significant public health burden in the tropics. Infection with malaria causing parasites results in a wide range of clinical disease presentations, from severe to uncomplicated or mild, and in the poorly understood asymptomatic infections. The complexity of asymptomatic infections is due to the intricate interplay between factor...
Article
Full-text available
Antimalarial drug resistance is a substantial impediment to malaria control. The spread of resistance has been described using genetic markers which are important epidemiological tools. We carried out a temporal analysis of changes in allele frequencies of 12 drug resistance markers over two decades of changing antimalarial drug policy in Kenya. We...
Data
PCR conditions and primer sequences for the gene regions analysed in the six erythrocyte and merozoite genes.
Data
Summary of whole genome Tajima's D values >1 from 3 previously published African parasite populations.
Article
Full-text available
Erythrocyte surface proteins have been identified as receptors of Plasmodium falciparum merozoite proteins. The ligand-receptor interactions enable the parasite to invade human erythrocytes, initiating the clinical symptoms of malaria. These interactions are likely to have had an evolutionary impact on the genes that encode the ligand and receptor...
Article
Full-text available
Background: Plasmodium falciparum infections lead to febrile illness unless the host has sufficient immunity, in which case infection may cause no immediate symptoms (i.e. "asymptomatic parasitaemia"). Previous studies are conflicting on the role of asymptomatic parasitemia in determining the risk of developing febrile malaria. Methods: We monit...
Article
Full-text available
The leading circumsporozoite protein (CSP) based malaria vaccine, RTS,S, though promising, has shown limited efficacy in field studies. There is therefore, still a need to identify other malaria vaccine targets. Merozoite antigens are potential vaccine candidates, since naturally acquired antibodies generated against them inhibit erythrocyte invasi...

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Projects

Project (1)
Project
Malaria is a life-threatening disease caused by parasites that are transmitted to people through the bites of infected female Anopheles mosquitoes. It is preventable and curable. The objective of this project is to use statistical modelling, molecular techniques and immunological techniques to study the complexity of parasite populations so as to inform malaria intervention efforts.