Keshavkant Sahu

Keshavkant Sahu
Pt. Ravishankar Shukla University · School of Studies in Bio-Technology

Doctor of Philosophy (Bioscience)

About

87
Publications
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1,153
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November 2012 - November 2016
Pt. Ravishankar Shukla University
Position
  • Professor

Publications

Publications (87)
Article
CONTEXT : Being sessile, it is impossible for the plants to evade from the unfavourable environmental conditions prevailing due to various abiotic stresses like heat, salinity, drought, flood, heavy metals, and high radiance among many others. These abiotic stresses disrupt plant growth and limit crop productivity to a large extent globally. Crop p...
Article
In this investigation, a novel electrochemically active Gram positive bacterium was isolated from the biofilm of a dual chambered microbial fuel cell (MFC) flooded with dairy wastewater (DWW), and was annotated as Enterococcus faecalis DWW1 following the 16 s rRNA sequencing. The dye-reduction based electron-transfer activity (DREAM) assay was used...
Article
Nitric oxide (NO) and hydrogen sulfide (H2S) are two versatile gaseous molecules which play myriad roles in the growth and development of plants. They play an important role in signal transduction process in plants exposed to various environmental stresses. Signal transduction and various antioxidant strategies are vital for the management of abiot...
Article
Modernization leads to frequent and remarkable alterations in the environmental factors which consequently affect the global agricultural productivity. Environmental factors can be broadly categorized in to biotic and abiotic components. Biotic factors, which are represented by pests, bacteria, viruses, fungi, nematodes, etc., pose a severe threat...
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Strigolactones (SLs) are carotenoid-derived molecules, which regulate various developmental and adaptation processes in plants. These are engaged in different aspects of growth such as development of root, leaf senescence, shoot branching, etc. Plants grown under nutrient-deficient conditions enhance SL production that facilitates root architecture...
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Approximately 20% of plant species, including Madhuca latifolia Roxb., produce recalcitrant seeds that show sensitivity towards desiccation and, not amenable to conventional seed storage techniques. This sensitivity is attributed to the poor expression of structural, biochemical and molecular mechanisms responsible for tolerance to desiccation in o...
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Metal-based nanoparticles (NPs) have recently been accomplished a great attention worldwide, in various sectors including agriculture due to their beneficial impacts in plant growth, development and stress tolerance. However, it shows dose dependent response and may vary with type of metal and synthesis procedure followed. Among many, silver nanopa...
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The ability of melatonin to regulate number of physiological and biochemical processes under different environmental stresses has been widely studied in plants. So, this investigation was done to study the protective roles of melatonin on Cicer arietinum L. grown under arsenic stress. Subjecting Cicer arietinum L. seeds to arsenic stress caused sig...
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A variety of synthesized hazardous substances are unethically regularly released in the natural environments by industrial, agricultural and several other profit making sectors. These xenobiotics possess unique features like longer persistence, extended half life, and get accumulate in the environment due to their chemical structure. Being a recalc...
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One of the important goals of sustainable living is to preserve the natural environment, and to minimize the harmful effects of man-made contaminants on ecosystems. Contaminants are the substances that are nondegradable, relatively recalcitrant, insoluble in water, nonpermeable to microbial cells, and are detrimental toward both lower and higher cl...
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With the rising inadequacy in arable land and water resources, the progress of agriculture sector is only possible by upsurging the resources use efficacy following least damage to agroecology via effective exploitation of modern technologies. Amid these, nanotechnology has the potential to revolutionize agricultural systems, biomedicine, environme...
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Nanotechnology is a broad term used to portray technologies functioning on nanoscales for their exploitation in real-world applications. The release of metal nanoparticles, specifically silver nanoparticles (AgNPs), in the natural environment has raised concerns about their distinguished effects on living organisms, including plants. Recent studies...
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This article reports the cryopreservation of apical meristems of Chlorophytum borivilianum, a tropical and IUCN critically endangered species. Initially, in vitro cultured shoots were pre-adapted on 12% (w/v) sucrose for 2-months and were found appropriate stock material for further experimentations. Furthermore, the preculture of meristems excised...
Article
Aluminum (Al) is considered as a potential limiting factor for plant growth in acidic environment. At lower concentration, Al promotes plant growth by facilitating the phosphorous availability, while, at higher concentration, it causes rhizotoxicity by inhibiting the nutrient transportation system. Cellular membrane is identified as the first site...
Article
Modern agricultural efforts are now in search of an efficient, eco-friendly and sustainable approach for enhanced crop production. Nearly 50–60% of seeds lost occurs due to improper technical handling. Seed deterioration manifests itself as reduction in the rate of germination and growth with increased susceptibility to biotic and abiotic stresses....
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Since the onset of the industrial revolution, metalloids are an exceptional class of toxicants that adversely affects plant growth and productivity when present in high concentrations in the agricultural soil. Various transporters are accountable for the entry and distribution of different elements inside the plant. Due to similar structural proper...
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Phytoremediation is a form of bioremediation which deals with the enormous potential of plants for degrading or immobilizing contaminants in soil and groundwater. Anthropogenic activities release huge amounts of chemicals, wastes, and noxious gases into the environment, which alter the physical, chemical, and biological properties of ecosystem, wor...
Chapter
A variety of synthesized hazardous substances are unethically and regularly released into the natural environments by industrial, agricultural, and several other profit-making sectors. These xenobiotics possess unique features such as longer persistence, extended half-life, and accumulation in the environment due to their chemical structure. Becaus...
Chapter
Environmental contamination by explosives like TNT, RDX, HMX, etc., due to their manufacturing, testing and disposal is becoming a matter of immense interest, worldwide. Most explosives are extremely hazardous to biological systems because of their toxicity and mutagenicity. Degradation of such contaminants by naturally occurring plants is often a...
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The effects of aluminium (Al)-induced alterations on elongating radicles of Cicer arietinum L. were studied in relation to growth and biochemical markers of oxidative stress. Elongating radicles (c. 1 mm) were treated with 0-3 mM aluminium chloride (pH 4.5) for seven days at room temperature (26 ± 2°C). The results indicated that exposure to Al sig...
Article
The scientific and technological applications of one of the nanomaterials viz.; carbon dot (C-dots), having extraordinary properties, is becoming an emerging and ongoing research area in recent times. In the present study, we have evaluated the effectiveness of C-dots in reducing arsenic (As) toxicity by analyzing physiological, biochemical and mol...
Chapter
Heavy metal/metalloid (HM) contamination issues are becoming progressively more widespread worldwide, which are witnessed in various locations like foundries, mining industries, smelters, vehicular emissions, coal burning power plants, and agricultural sectors. Heavy metal/metalloid occurs naturally in the Earth’s crust, but man-made sources and va...
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Metal/metalloid (HM) toxicity/stress has become a worldwide menace due to the rising accretions in water, soil, and air which lead to detrimental effects in plants. The general consequences of HM toxicity include oxidative injury which causes polypeptide oxidation, lipid peroxidation, enzyme inactivation, DNA mutilation, and/or alteration of other...
Article
Heavy metal pollution and acid rain are serious global environmental issues. The combined pollution of these two contaminants has become a new environmental issue where both stresses occur simultaneously. These abiotic stresses alter the plant growth and development, various metabolic activities and agricultural yield. However, studies on combined...
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Arsenic (As) resistant indigenous bacteria with discrete minimum inhibitory concentration values for arsenate [As(V)] and arsenite [As(III)] were isolated from the paddy fields of different regions of Chhattisgarh, India, following enrichment culture technique. Evaluation of the plant growth promoting (PGP) properties of the isolates revealed that...
Article
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Fluoride (F), anion of fluorine which is naturally present in soil and water, behaves as toxic inorganic pollutant even at lower concentration and needs immediate attention. Its interaction with flora, fauna and other forms of life, such as microbes, adversely affect various physiochemical parameters by interfering with several metabolic pathways....
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Arsenic (As)-toxicity is recognized as one of the major environmental problems, affecting productivity of crops worldwide, thereby threatening sustainable agriculture and food security. Progression in nanotechnology and its impacts have brought up concerns about the application of engineered nanoparticles (NPs) in various sectors of the economy, in...
Article
Arsenic (As)-toxicity is recognized as one of the major environmental problems, affecting productivity of crops worldwide, thereby threatening sustainable agriculture and food security. Progression in nanotechnology and its impacts have brought up concerns about the application of engineered nanoparticles (NPs) in various sectors of the economy, in...
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In recent era, due to climate change/ global warming plants are constantly stumbling upon inauspicious growth conditions. Maintenance of transitory or constantly high temperature in the surrounding environment results into alterations in plants at physiological, biochemical and molecular levels, which in turn influences growth, development and yiel...
Article
Enhanced cellular damage during desiccation is considered to be one of the key factors limiting vigour and viability of seeds. The uncontrolled accumulation of reactive oxygen species and resultant damaging reactions like oxidation of lipids and DNA in desiccating Madhuca latifolia seeds were well characterized previously. However, hydrolytic and/o...
Article
Nanoparticles (NPs) and nano-technological applications in varied economic sectors including agriculture, accomplished great attention in last decades, worldwide. In several instances, NPs has been applied as pre-treatment or toxicant scavenging agent for promoting seed germination/ plant growth responses or for abiotic stress amelioration. In orde...
Article
A total of forty‐five morphologically distinct arsenic (As) resistant bacterial strains were isolated from the soils of different regions of Chhattisgarh, India. The minimum inhibitory concentration (MIC) values of these isolates varied widely in the range of 100‐500 mM for arsenate (As(V)) and 15‐30 mM for arsenite (As(III)). Out of forty‐five, th...
Article
Lead and acid rain are important abiotic stress factors that limit the growth, development, metabolic activity and yield of the crops. Melatonin (MT; an indoleamine molecule), glutathione (GSH; free thiol tripeptide) and thiourea (TU; non physiological thiol based ROS scavenger) have been known to mediate several physiological, biochemical and mole...
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Abstract The storage of recalcitrant seeds represents a challenge globally, threatening the germplasm conservation of many economically important tropical and sub-tropical species. This study investigated whether inhibitors of reactive oxygen species (ROS), diphenyleneiodonium (DPI) and dimethyl thiourea (DMTU), and hydrated storage can enhance the...
Article
Dimethylthiourea is an important plant growth regulator that mediates various physiological and metabolic processes of the plants. In the present study, role of dimethylthiourea in conferring arsenic stress tolerance to Cajanus cajan L. was investigated. Exposure to arsenic resulted in oxidative damage as evidenced by decreased germination percenta...
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Being regulators of growth, both spermidine (Spd) and melatonin (Mel) are involved actively in the modulation of abiotic stress responses of plants. Hence, the present study was aimed to scrutinize the possible involvements of Spd and Mel in alleviation of fluoride ion (F⁻)-induced injuries in Cajanus cajan L. Seeds of C. cajan L. were exposed to 1...
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Understanding ill effects of simultaneous existence of various abiotic stresses, commonly observed due to various anthropogenic activities and global climate change these days, over plants growth, metabolic activity and yield responses are important for continued agricultural productivity and food security. In the present study, seedlings of Fenugr...
Article
Arsenic (As) is a toxic metalloid, severely affecting the normal functioning of plants, animals, and human beings. Nitric oxide (NO) and dimethylthiourea (DMTU) are two potential compounds play key roles in enhancing tolerance against a variety of abiotic stresses in plants. In the present study, ameliorating abilities of both NO and DMTU against A...
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Arsenic (As), a non-essential metalloid and severely toxic to all the living organisms exists mainly in two inorganic forms arsenate (AsV) and arsenite (AsIII). Arsenic is known to cause deleterious impacts on growth and metabolism of plants chiefly via slowing down the cell division and elongation, increased formation of reactive oxygen species (R...
Article
Diosgenin, one of the steroidal sapogenin, is a chief bioactive compound, commercially known for its pharmaceutical application to treat sexual dysfunction. The biosynthesis of plant secondary metabolites in vitro cultures is usually manipulated by different elicitors that lead to higher production than non-elicited cultures. In the present investi...
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Arsenic (As)-toxicity is a major constraint for crop production. The present study was intended to examine the comparative ameliorative effects of diphenyleneiodonium (DPI), 24-epibrassinolide (EBL) and proline (Pro) on As-stress in Glycine max L. Seeds of Glycine max L. were subjected to As (100 μM) singly, and together with DPI (10 μM), EBL (0.5...
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Key message Storage longevity of two contrasting recalcitrant seeded species was studied relative to oxidative metabolism and DNA damage under ambient storage (25 ± 2 °C, 50 ± 2% relative humidity). Abstract The present study was aimed to investigate the ageing-induced amendments in two recalcitrant seeded species, Madhuca latifolia and Shorea rob...
Chapter
Arsenic (As) pollution, particularly in soil and water, is a very prominent environmental issue which seriously threatens plant growth, development, and productivity. Since As is ubiquitous in the natural environment, microorganisms have developed mechanisms to resist the toxic effects of this metalloid. A large number of microorganisms, viz. Acine...
Chapter
Arsenic (As) is a naturally occurring toxic metalloid, ubiquitously present in the environment. It enters the environment from both geogenic and anthropogenic sources. Arsenic accumulates to different edible tissues and thereby enters into food chain. Arsenate and arsenite are two main phyto-available forms of As and are popularly reported to cause...
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Germinated Pennisetum typhoideum seeds were grown under phytotoxic amount of nickel (Ni) and its combinations with aspirin (Asp) and/or glycinebetaine (GB). The results revealed that exposure to Ni caused reduced growth and membrane stability index of P. typhoideum, which were correlated with the accumulated Ni and reactive oxygen species. Oxidativ...
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Increased anthropogenic environmental pollution, one of the serious threats associated with rapid industrial/ economic development leads to enhanced release of SOX and NOX in the troposphere which later combined with moisture and results in acid rain (AR). Recurrence of AR leads an array of alterations in plants that includes inhibited seed germina...
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Loss of viability in desiccation-sensitive seeds of Madhuca latifolia (Roxb.) J. F. Macbr., an important multipurpose tropical tree, was correlated with seed water content (WC). WC declined from 0.59 to 0.19 g g⁻¹ fresh mass, 35 days after harvest from mother plant, at ambient conditions (temperature 25 ± 2 °C, relative humidity 50 ± 2%). The desic...
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Excess of fluoride (F) in irrigation water is a serious threat inducing variety of deleterious impacts in plants via over-production of active oxygen species (AOS), modifying antioxidant enzymes and nucleic acid. Present study was aimed to monitor protective functions of glycinebetaine (GB) against F-toxicity in Cajanus cajan L. GB is an osmolyte,...
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Proline, 24-epibrassinolide and diphenylene iodonium are few of the novel antioxidant molecules, involved in growth regulation and abiotic stress tolerance of plants. However, these are scarcely explored in relation to their role in arsenic stress tolerance. Therefore, present study was designed to investigate the involvement of proline, 24-epibras...
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Activities of soil enzymes are closely associated with its fertility. Extensive agricultural practices firmly based on unethical pouring of agrochemicals, which adversely affects soil microflora, and interferes with their associated enzymatic activities. Therefore, current study was aimed to monitor single and combined impacts of insecticides chlor...
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Full-text available
Presence of the toxic metalloid, " arsenic (As) " is ubiquitous in the environment especially in the soil and water. Its excess availability in the soil retards growth and metabolism of plants via (a) slowing down the cell division/elongation, (b) overproduction of reactive oxygen species (ROS), (c) modulation of antiox-idant enzymes, and (d) alter...
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This study was undertaken to scrutinize efficacy of salicylic acid (SA) and/or sodium nitroprusside [SNP, source of nitric oxide (NO)] to mitigate injury symptoms of saline stress in Pisum sativum L. Exposure to sodium chloride (NaCl) was found to be injurious to germinating P. sativum L. (var. Shubhra IM-9101) and a direct correlation between seve...
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This study was undertaken to scrutinize efficacy of salicylic acid (SA) and/or sodium nitroprusside [SNP, source of nitric oxide (NO)] to mitigate injury symptoms of saline stress in Pisum sativum L. Exposure to sodium chloride (NaCl) was found to be injurious to germinating P. sativum L. (var. Shubhra IM-9101) and a direct correlation between seve...
Article
Full-text available
The fluoride ion (F) is widespread in the environment and toxic for all living organisms. Prolonged exposure to F leads to physiological, biochemical, and molecular modifications in plants. F is known as a potential metabolic inhibitor, interfering with the overall responses of plants including seed germination, growth and productivity, biomass acc...
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To investigate the physiological and metabolic attributes of arsenic (As) stress tolerance conferred by exogenous salicylic acid (SA), Glycine max L. (variety JS 335) seeds were aseptically germinated over filter paper moistened with SA (500 μM) and/or10 and 100 μM As (Sodium arsenite was used). On 2nd and 5th days of germination, the growing radic...
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In recent decades acid rain (AR) has emerged as a matter of the serious environmental threats, hence is of global concern. It has been described as one of the peril coupled with the processes of industrialization and more recently climate change. Intensified air pollution and its adverse effects on natural vegetation have popularly been reported al...
Article
Full-text available
Fluoride (F) is widespread in the natural environment and toxic for all living organisms including plants. It exists in the soil, water, air and also in plants with varying concentrations. Excessive availability of F (permissible limit 1.5 ppm, WHO) leads to contamination in water, soil and detrimental impacts in plants, and is a serious health haz...
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Soil microbial community plays a significant role in soil ecosystem.Soil microorganisms are the key activators of various biogeochemical cycles of the nature and play an affirmative role in maintaining fertility and health of the soil by releasing soil enzymes. These are also helpful in decomposition of organic residues, circulation of nutrients, f...
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Seed development comprises a series of events involving cell division/histo-differentiation, reserves deposition and desiccation. Development of Madhuca (Madhuca latifolia Roxb.) seed has been divided into eight consecutive stages; 10, 18, 26, 34, 42, 50, 58, 66 days after fertilization (DAF). Seed colour was finally found to turn dark-brown from i...
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Genus Chlorophytum Ker Gawl secures its position chiefly as commercial plants with a wide range of applications, right from pharmaceutical to ornamental, and with a promising economical return also. Few species of this genus are now enlisted under “threatened plant category” due to rash harvesting from its wild habitat, as it is utilized extensivel...
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Arsenic (As) is one of the highly toxic metalloids distributed ubiquitously in nature. Two inorganic forms of As are present abundantly: arsenite (AsIII) and arsenate (AsV), former being 100 times more toxic than latter. Arsenic is a well known inducer of reactive oxygen species (ROS) in crop plants either directly during conversion of AsV to AsIII...
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A simple, in vitro multiplication and mid-term conservation system was developed for Chlorophytum borivilianum Sant. et Fernand, which is documented as a critically endangered herb on the International Union for Conservation of Nature and Natural Resources red list. In vitro shoot multiplication was achieved by culture on Murashige and Skoog medium...
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Reactive oxygen species (ROS) play key roles in seed germination and seedling establishment and was shown to perform both beneficial and damaging roles, depending upon concentration. At low level, it has been shown to boost plant growth, while high existence is injurious. In view to investigate the accumulation and consequent functions of ROS durin...
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Full-text available
Reactive oxygen species play key roles in seed germination and seedling establishment and was shown to perform both beneficial and injurious functions, depending upon concentration. At low amount, it has been shown to boost plant growth, while high existence is injurious. In view to investigate the accumulation and consequent functions of ROS durin...
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Full-text available
Protein extraction for two-dimensional electrophoresis from tissues of recalcitrant species is quite problematic and challenging due to the low protein content and high abundance of contaminants. Proteomics in Shorea robusta is scarcely conducted due to the non-availability of suitable protein preparation procedure. To establish an effective protei...
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Orthodox seed serves as easily accessible model to study desiccation-sensitivity in plant tissues because once they undergo germination, they become sensitive to desiccation imposed injuries. In the proposed study, effects of rate of drying on the viability, electrolyte leakage, superoxide accumulation, lipid-protein oxidation and antioxidant enzym...
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Seeds of Shorea robusta (sal) are recalcitrant owing to its high desiccation sensitivity. Germinability in sal seed was lost rapidly from 100 to 0 % within 8 days. Protein oxidation examined separately in axis and cotyledon of ageing sal seeds by monitoring the levels of carbonyls, hydroperoxide, malondialdehyde and 4-hydroxy-2-nonenal adducts with...