Kenneth J Shea

Kenneth J Shea
University of California, Irvine | UCI ·  Department of Chemistry

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447
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Publications

Publications (447)
Article
Porous membranes have been used for many applications, including separations in biotechnology, the food industry, water purification, and even energy storage devices. The benefit of polymers of intrinsic microporosity (PIMs) is their consistently sized nanopore channels. Inherent functionalities of the PIM structure not only create these channels b...
Article
Full-text available
Sepsis is a life-threatening condition caused by the extreme release of inflammatory mediators into the blood in response to infection (e.g., bacterial infection, COVID-19), resulting in the dysfunction of multiple organs. Currently, there is no direct treatment for sepsis. Here we report an abiotic hydrogel nanoparticle (HNP) as a potential therap...
Article
Full-text available
This study analyzes the swelling behavior of native, unmodified, spherically uniform, monodisperse poly(lactic-co-glycolic acid) (PLGA) microparticles in a robust high-throughput manner. This work contributes to the complex narrative of PLGA microparticle behavior and release mechanisms by complementing and extending previously reported studies on...
Article
We report a metal free synthetic hydrogel copolymer with affinity and selectivity for His6-tagged peptides and proteins. Small libraries of copolymers incorporating charged and hydrophobic functional groups were screened by an iterative process for His6 peptide affinity. The monomer selection was guided by interactions found in the crystal structur...
Article
In this paper, the soft thermosensitive photonic crystals can be immobilized by reversible temperature-triggered in-situ sol-gel transition above phase transition temperature (Tp), which may be a significant advance in the related field. Specifically, a library of thermosensitive poly(N-isopropylacrylamide)/poly(acrylic acid) (PNIPAm/PAA) interpene...
Article
Peptide-polymer complementary pairs can provide useful tools for isolating, organizing, and separating biomacromolecules. We describe a procedure for selecting a high affinity complementary peptide-polymer nanoparticle (NP) pair using phage display. A hydrogel copolymer nanoparticle containing a statistical distribution of negatively charged and hy...
Preprint
Full-text available
Sepsis is a life-threatening condition caused by the extreme release of inflammatory mediators into the blood in response to infection (e.g., bacterial infection, COVID-19), resulting in the dysfunction of multiple organs. Currently, there is no direct treatment for sepsis. Here we report an abiotic hydrogel nanoparticle (HNP) as a potential therap...
Article
Full-text available
Synthetic polymer hydrogel nanoparticles (NPs) were developed to function as abiotic affinity reagents for fibrinogen. These NPs were made using both temperature-sensitive N-isopropyl acrylamide (NIPAm) and L-amino acid monomers. Five kinds of L-amino acids were acryloylated to obtain functional monomers: L-phenylalanine (Phe) and L-leucine (Leu) w...
Article
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Linear aliphatic oligoesters derived from δ-valerolactone (VL) and ε-caprolactone (CL) were synthesised by ring-opening polymerisation (ROP) using 1-docosanol (C22H45OH, C22OH) and aluminum isopropoxide [Al(OⁱPr)3] as initiator and catalyst, respectively. Monodisperse species such as monomers, dimers, trimers, and tetramers with terminal groups α-h...
Article
It is significant to detect lysozyme both in fundamental research and in clinical practice, since its concentration in body fluid is associated with many diseases. In this study, a new kind of sensor for rapid screening the lysozyme was fabricated based on the two-dimensional (2D) polystyrene colloidal crystal array together with the optimized hydr...
Article
Konjac tofu is a traditional Chinese food whose main ingredient is the water-soluble polysaccharide from konjac tubers-konjac glucomannan (KGM). Herein we report an interpenetrating double network polymeric film by crosslinking KGM and polyvinyl alcohol (PVA) with glutaraldehyde under acidic conditions. PVA was introduced into the hydrogel system a...
Article
We describe a process for engineering a synthetic polymer nanoparticle (NP) that functions as an effective, broad-spectrum metalloproteinase inhibitor. Inhibition is achieved by incorporating three functional elements in the NP; a group that interacts with the catalytic zinc ion, functionality that enhances affinity to the substrate-binding pocket...
Article
The affinity of a synthetic polymer nanoparticle (NP) to a target biomacromolecule is determined by the association and dissociation rate constants (kon, koff) of the interaction. The individual rates and their sensitivity to local environmental influences are important factors for the on demand capture and release a target biomacromolecule. Positi...
Article
A wearable silk fibroin (SF) / cellulose composite is reported. It is structural dyed and functionalized by embedding three dimensional (3D) or two dimensional (2D) poly (methyl methacrylate) (PMMA) and polystyrene (PS) nano colloidal arrays to form opal and inverse opal silk methylcellulose photonic crystal films (SMPCF). The brilliant color of SM...
Article
Incorporation of metal nanoparticles into metal-organic framework (MOF) nanosheets have been deemed as a promising heterogeneous catalyst. We report the synthesis of ultra-thin two-dimensional MOF nanosheets for loading Pd nanoparticles...
Article
Full-text available
Stimuli responsive polymers are an efficient means of targeted therapy. Compared to conventional agents, they increase bioavailability and efficacy. In particular, polymer hydrogel nanoparticles (NPs) can be designed to respond when exposed to a specific environmental stimulus such as pH or temperature. However, targeting a specific metabolite as t...
Article
Protein affinity reagents (PARs), frequently antibodies, are essential tools for basic research, diagnostics, separations and for clinical applications. However, there is growing concern about the reproducibility, quality and cost of recombinant and animal-derived antibodies. This has prompted the development of alternatives that could offer econom...
Article
Full-text available
Envenomings by snakebites constitute a serious and challenging global health issue. The mainstay in the therapy of snakebite envenomings is the parenteral administration of animal-derived antivenoms. Significantly, antivenoms are only partially effective in the control of local tissue damage. A novel approach to mitigate the progression of local ti...
Data
NP selectivity experiment for Bungarus fasciatus venom (blue) and Naja haje venom (red) using 1% (w/w) venom in 25% human serum. (TIF)
Data
NP selectivity experiment for Naja mossambica venom (red) using 1% (w/w) venom in 25% human serum. (TIF)
Data
NP selectivity experiment for Bungarus caeruleus venom (red) using 1% (w/w) venom in 25% human serum. (TIF)
Data
Gel bands selected for trypsin digestion and LC-MS/MS proteomics analysis for Bungarus caeruleus selectivity experiment. (TIF)
Data
Gel bands selected for trypsin digestion and LC-MS/MS proteomics analysis for Dendroaspis polylepis selectivity experiment. (TIF)
Data
Macroscopic assessment of the inhibition of dermonecrotic activity of N. nigricollis venom by NPs. Venom and NPs were mixed at a NP: venom ratio of 5.0 (w/w) and incubated for 30 min at room temperature. Controls included venom incubated with saline solution (venom) and NPs incubated with saline solution (nanoparticles). Then, aliquots of each mixt...
Data
Identification of toxins in Naja mossambica venom experiment using digested SDS-PAGE gel bands depicted in S7 Fig. (TIF)
Data
Light micrographs of sections from heart (A), liver (B), kidney (C) and skeletal muscle (D) from mice injected with nanoparticles. Mice (18–20 g body weight) were injected with either 100 μL of nanoparticles in saline solution (5.5 mg/mL) by the intravenous (i.v.) route in the caudal vein, or intramuscularly (i.m.) in the right gastrocnemius. Mice...
Data
NP selectivity experiment for Naja nivea venom (blue) and Naja melanoleuca venom (red) using 1% (w/w) venom in 25% human serum. (TIF)
Data
NP selectivity experiment for Naja sputatrix venom (red) using 1% (w/w) venom in 25% human serum. (TIF)
Data
NP selectivity experiment for Dendroaspis polylepis venom (blue) using 1% (w/w) venom in 25% human serum. (TIF)
Data
Gel bands selected for trypsin digestion and LC-MS/MS proteomics analysis for Naja mossambica selectivity experiment. (TIF)
Data
The cell viability of L6 cells incubated with Naja nigricollis venom (1–30 μg/mL, n = 4–5) in the presence (black square) and absence (black circle) of 80 μg/mL nanoparticle, respectively. (TIF)
Data
Identification of toxins found in Bungarus caeruleus experiment using digested SDS-PAGE gel bands depicted in S8 Fig. (TIF)
Data
Gel bands selected for trypsin digestion and LC-MS/MS proteomic analysis for Naja nigricollis selectivity experiment. (TIF)
Data
Identification of toxins found in D. polylepsis experiment using digested SDS-PAGE gel bands depicted in S9 Fig. (TIF)
Data
The excel files for each set of MS-MS experiments. Note the tabs on the bottom of the files, which correspond to the gel band (peptide summary and protein summary—2 tabs per gel band). In every instance the uniprot database corresponding to the snake species of interest was included in the search. However, the total database used in the search vari...
Data
Identification of toxins found in Naja nigricollis experiment using digested SDS-PAGE gel bands depicted in S10 Fig. (TIF)
Article
We report a novel strategy for creating abiotic Bacillus thuringiensis (Bt) protein affinity ligands by biomimicry of the recognition process that takes place between Bt Cry1Ab/Ac proteins and insect receptor cadherin-like Bt-R1 proteins. Guided by this strategy, a library of synthetic polymer nanoparticles (NPs) was prepared and screened for bindi...
Article
Full-text available
Traditional artificial opals are assembled from silica or polystylene colloidals which have poor swellability and a lower response to stimuli. A novel three-dimensional photonic crystal array sensor which has a high stability and desired structural colour was fabricated from the self assembly of nano hydrogel colloids. The nano hydrogel colloids we...
Article
A series of redox- and photo-responsive copolymers, poly(2-(methacryloyloxy)ethyl ferrocenecarboxylate-co-4-methacryloylamino-4′-nitroazobenzene) (P(FcEMA-co-MAAzo)s) were synthesized using free radical polymerization. The synthesized copolymers were characterized by ¹H NMR, FT-IR, gel permeation chromatography (GPC) and thermogravimetric analysis...
Article
Redox/temperature responsive poly(N-isopropylacrylamide/N-hydroxyethylacrylamide) (P(NIPA/HEAA)) nonionic nanogels (NP-BAC) crosslinked with N,N′-Bis(acryloyl)cystamine (BAC) have been prepared. An analogous nanogel incorporating N,N′-methylenebisacrylamine (BIS), a reduction-insensitive nanogel (NP-BIS), was chosen as a control nanogel. Compared t...
Article
In this work, we demonstrated a nano-decorated porous impedance electrode sensor for efficient capture, rapid killing and ultrasensitive detection of bacteria. The multi-functional sensor was prepared by a facile sonochemical method via in situ deposition of antibacterial prickly Zn-CuO nanoparticles and graphene oxide (GO) nanosheets on a Ni porou...
Article
Full-text available
Diblock copolymers of poly(methylene-b-ε-caprolactone) (PM-b-PCL) were synthesized in two steps: a) polyhomologation, to obtain following oxidative cleavage of the carbon-boron, (PM, block1), an α-hydroxyl-ω-methyl polymethylene (PMOH, CH3−[CH2]m−OH). Following transfer reaction to the ring-opening polymerization catalyst and b) ring-opening polyme...
Article
Three-dimensional photonic crystal sensors are attractive platforms for autonomous chemical sensing and colorimetric bioassays. At present, the photonic crystal sensors with inverse opal structure were extensively studied, which swells or shrinks in response to the analytes. However, the fabrication of inverse opal sensors still remains a major cha...
Article
Protein affinity reagents are widely used in basic research, diagnostics and separations and for clinical applications, the most common of which are antibodies. However, they often suffer from high cost, and difficulties in their development, production and storage. Here we show that a synthetic polymer nanoparticle (NP) can be engineered to have m...
Article
Biochemical diversity of venom extracts often occurs within a small number of shared protein families. Developing a sequestrant capable of broad-spectrum neutralization across various protein isoforms within these protein families is a necessary step in creating broad-spectrum antivenom. Using di-rected synthetic evolution to optimize a nanoparticl...
Article
Full-text available
Specific cell adhesion and osteogenicity are both crucial factors for the long-term success of titanium implants. In this work, two mussel-derived bioactive peptides were designed to one-step dual-biofunctionalization of titanium implants via robust catechol/TiO2 coordinative interactions. The highly biomimetic peptides capped with integrin-targete...
Article
A facile controlled-release nanogels delivery system has been developed by using hydroxypropyl methylcellulose (HPMC) hybrid nanogels as encapsulation shell materials, which were synthesized by surfactant-free polymerization in aqueous solution. The effects of reaction time and cross-linker concentration on the size of the nanogels have been studie...
Article
Poly(1,4-butanediol dimethacrylate) microcapsules containing dementholized peppermint oil (DPO) were prepared by interfacial free-radical polymerization. The influence of the composition of the core material on the morphology of the fragrance microcapsules was investigated by scanning electron microscopy (SEM). Response surface methodology (RSM) wa...
Article
The self-assembled fabrication of large-area opal photonic crystals (PCs) that are free of cracks has remained a challenge which greatly limits the practicality of such a structure in optical and electronic applications. We report a new route in this paper for fabricating centimeter scale crack-free opal PC films in which the latex spheres are boun...
Article
The specific binding and uptake of protein molecules to individual hydrogel nanoparticles is measured with real-time single-nanoparticle surface plasmon resonance imaging (SPRI) microscopy. Nanoparticles that adsorb onto chemically modified gold thin films interact with traveling surface plasmon polaritons and create individual point diffraction pa...
Article
The insertion of long hydrocarbon chains between ester groups in polyester allows for introduction and modulation of crystallinity, which can result in enhanced performance approaching high-density polyethylene. We report a convenient synthesis of building blocks for long-chain aliphatic polyesters via an aqueous C1 polymerization. The macromonomer...
Article
Nanomaterials, when introduced into a complex, protein-rich environment, rapidly acquire a protein corona. The type and amount of proteins that constitute the corona depend significantly on the synthetic identity of the nanomaterial. For example, hydrogel nanoparticles (NPs) such as poly(N-isopropylacrylamide) (NIPAm) have little affinity for plasm...
Article
A pH-responsive bovine serum albumin molecularly imprinted poly(ionic liquids) was prepared on the surface of multiwall carbon nanotubes via a sol-gel route. The specific binding from shape selectivity was significantly strengthened by controlling the non-specific binding by adjusting the incubation pH value to 9.9. The use of ionic liquids as stab...
Article
Hydrophobic interactions often dominate the associative forces between biomacromolecules. A synthetic affinity reagent must be able to exploit and optimize these interactions. We describe synthesis of abiotic affinity reagents that sequester biomacromolecules with lipid-like domains. NIPAm based copolymer nanoparticles (NPs) containing C4-C8 hydrop...
Article
Designed polymer hydrogel nanoparticles (NPs) capable of facilitating resolubilization and refolding of an aggregated protein, positively charged lysozyme, are prepared. NPs designed to interact strongly with denatured lysozyme and relatively weakly with native lysozyme, facilitated resolubilization and refolding of aggregated lysozyme. Such NPs co...
Article
Full-text available
Molecularly imprinted polymers (MIPs) are highly cross-linked polymers with high binding capacity and selectivity to the target molecules. MIPs become increasingly important because of the potential applications in drug delivery, purification and separation. In spite of the tremendous progress that has been made in the molecular imprinting field, m...
Article
In this paper, a pseudo-ternary phase diagram was drawn according to the phase separation theory to decide if O/W capsules can be formed by using poly(ethylene glycol dimethacrylate) as shell material and fragrance dementholized peppermint oil as model core material. Within a certain ratio range of reaction reagents, the nanocapsule was first synth...
Article
A new kind of phase change material n-octadecane microcapsules containing peppermint fragrance scent has been synthesized through the interfacial polymerization of isophorone diisocyanate (IPDI) and hexamethylenediamine (HMDA). The average size, morphology, and chemical components of the microcapsules were characterized by laser particle size analy...
Article
The sensitivity of long persistent phosphor materials (LPP) to moisture greatly limits their applications especially in humid environments, which cause the hydrolysis of LPP and shorten their lifetime. In this paper, a facile, environmentally friendly and low-cost method was developed to prevent the infiltration of water or moisture to the LPP by d...
Article
Following its resolution by diastereomeric complexation, 5,5′,6,6′-tetrahydroxy-3,3,3′,3′-tetramethyl-1,1′-spirobisindane (TTSBI) was used to synthesize a chiral ladder polymer, (+)-PIM-CN. (+)-PIM-COOH was also synthesized by the acid hydrolysis of (+)-PIM-CN. Following characterization, both (+)-PIM-CN and (+)-PIM-COOH were solvent cast directly...
Article
Following its resolution by diastereomeric complexation, 5,5′,6,6′-tetrahydroxy-3,3,3′,3′-tetramethyl-1,1′-spirobisindane (TTSBI) was used to synthesize a chiral ladder polymer, (+)-PIM-CN. (+)-PIM-COOH was also synthesized by the acid hydrolysis of (+)-PIM-CN. Following characterization, both (+)-PIM-CN and (+)-PIM-COOH were solvent cast directly...
Article
Toxins delivered by envenomation, secreted by microorganisms, or unintentionally ingested can pose an immediate threat to life. Rapid intervention coupled with the appropriate antidote is required to mitigate the threat. Many antidotes are biological products and their cost, methods of production, potential for eliciting immunogenic responses, the...
Article
We report the first synthesis of a gradient methylidene-ethylidene copolymer via a living C1 polymerization. The copolymer has a similar chemical structure as the corresponding ethylene-propylene copolymer. To achieve this goal a new and convenient source of the ethylide monomer, diethylsulfoxonium ethylide, was developed for the introduction of th...
Article
Full-text available
Solid phase extraction (SPE) is widely used in many different areas, such as environmental, biological, and food analysis, where cleaning and pre-concentration of samples are key steps in the analytical protocol. New materials have significant impact on the development of solid phase extraction. In this paper, mono-dispersed molecularly imprinted h...
Article
This paper describes how changes in the refractive index of single hydrogel nanoparticles (HNPs) detected with near-infrared surface plasmon resonance microscopy (SPRM) can be used to monitor the uptake of therapeutic compounds for potential drug delivery applications. As a first example, SPRM is used to measure the specific uptake of the bioactive...
Article
Synthetic polymer nanoparticles (NPs) with intrinsic affinity for target biomacromolecules hold great promise in the development of novel tools for biological and biomedical research. We recently reported the design and synthesis of abiotic, synthetic polymer NPs with high intrinsic affinity for a peptide toxin melittin. The NP was selected by scre...
Patent
Full-text available
The present invention relates to compositions and methods for synthesis of organic-silica hybrid microparticles and nanoparticles. In particular, the present invention provides compositions and methods for particle size control during synthesis of organic-silica hybrid microparticles and nanoparticles.
Article
Herein is described an application of the acyl nitroso type 2 intramolecular Diels-Alder reaction as a key step in the progress towards the total synthesis of the Stemona alkaloid (-)-Stenine. The chiral cycloaddition precursor is rapidly assembled from a cyclohexene intermediate synthesized through an asymmetric, organocatalyzed Robinson annulatio...
Article
Full-text available
In this paper, a library of multi-functional linear poly-N-isopropylacrylamide (pNIPAm) polymers having a range of molecular weights and functional groups were synthesized and their interaction with the hemolytic peptide, melittin, was examined. The linear pNIPAm (LPs) containing both tert-butyl group and carboxylic acids bound with the peptide by...
Article
Full-text available
Single pores in resistive-pulse technique have been successfully used for the detection of cells, viruses, particles, and even molecules such as DNA and proteins. We have investigated application of pores with undulating opening diameter for the detection of particles and characterization of their physical and mechanical properties including size,...