Kenji Hashimoto

Kenji Hashimoto
Tokyo University of Science | TUS · Department of Applied Biological Science

About

57
Publications
8,269
Reads
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3,710
Citations
Citations since 2016
19 Research Items
2631 Citations
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
Additional affiliations
October 2005 - March 2014
University of Münster
Position
  • PostDoc Position

Publications

Publications (57)
Article
Excessive Na⁺ in soils inhibits plant growth. Here, we report that Na⁺ stress triggers primary calcium signals specifically in a cell group within the root differentiation zone, thus forming a “sodium-sensing niche” in Arabidopsis. The amplitude of this primary calcium signal and the speed of the resulting Ca²⁺ wave dose-dependently increase with r...
Chapter
Reactive oxygen species (ROS) produced by plant NADPH oxidases, respiratory burst oxidase homologs (RBOHs), play key roles in biotic and abiotic stress responses and development in plants. While properly controlled amounts of ROS function as signaling molecules, excessive accumulation of ROS can cause undesirable side effects due to their ability t...
Article
Full-text available
The two-pore channel (TPC) family is widely conserved in eukaryotes. Many vascular plants, including Arabidopsis and rice, possess a single TPC gene which functions as a slow vacuolar (SV) channel—voltage-dependent cation-permeable channel located in the vacuolar membrane (tonoplast). On the other hand, a liverwort Marchantia polymorpha genome enco...
Article
Full-text available
Sex determination is a central process for sexual reproduction and is often regulated by a sex determinant encoded on a sex chromosome. Rules that govern the evolution of sex chromosomes via specialization and degeneration following the evolution of a sex determinant have been well studied in diploid organisms. However, distinct predictions apply t...
Article
Full-text available
Inhibitors of human two-pore channels (TPC1 and TPC2), i.e., verapamil, tetrandrine, and NED-19, are promising medicines used in treatment of serious diseases. In the present study, the impact of these substances on action potentials (APs) and vacuolar channel activity was examined in the aquatic characean algae Nitellopsis obtusa and in the terres...
Article
Recent development of stimulated Raman scattering (SRS) microscopy allows for label-free biological imaging with chemical specificity based on molecular-vibrational signatures. In particular, hyperspectral SRS imaging can acquire molecular-vibrational spectrum at each pixel, allowing us not only to investigate the spectral difference of various bio...
Article
Full-text available
We developed a near-infrared laser-induced surface deformation (NIR-LISD) microscope and applied it to the dynamic relaxation measurements of a living plant surface at single-cell level. In the microscope, the surface deformation is induced by a NIR laser beam, then the changes in intensity of probe beam reflected from the surface are measured. The...
Article
Full-text available
Arbuscular mycorrhizal fungi and rhizobia association with plants are two of the most successful plant-microbe associations that allow the assimilation of P and N by plants, respectively. These mutualistic interactions require a molecular dialogue, i.e., legume roots exude flavonoids or strigolactones which induce the Nod factors or Myc factors syn...
Article
A near-infrared laser-induced surface deformation (NIR-LISD) microscope is developed and is applied to the dynamic viscoelastic measurements of the surface of a living plant cell. In the microscope, the deformation of the surface is induced by an NIR laser beam, and then the change in intensity of the probe beam reflected from the surface reflects...
Article
Inter-cellular and inter-kingdom signaling systems of various levels of complexity regulate pathogenic and mutualistic interactions between bacteria, parasites, and fungi and animal and plant hosts. Inter-kingdom interactions between mutualistic bacteria such as rhizobia and legumes during nodulation and between fungi and plants during mycorrhizal...
Article
Abscisic acid (ABA) regulates growth‐ and developmental processes in response to limiting water conditions. ABA functions through a core signaling pathway consisting of PYR1/PYL/RCAR ABA receptors, type 2C protein phosphatases (PP2Cs) and SnRK2‐type protein kinases. Other signaling modules might converge with ABA signals through the modulation of c...
Article
RBOHF from Arabidopsis thaliana represents a multifunctional NADPH oxidase regulating biotic and abiotic stress tolerance, developmental processes and guard cell aperture. The molecular components and mechanisms determining RBOHF activity remain to be elucidated. Here we combined protein interaction studies, biochemical and genetic approaches with...
Article
Full-text available
Root hairs (RH) are tip growing polarized cells aiding the uptake of nutrients and water into plants. RH differentiation involves the interplay of various hormones and second messengers. Tightly regulated production of reactive oxygen species by the NADPH oxidase RBOHC crucially functions in RH differentiation and Ca2+ -dependent phosphorylation ha...
Article
Full-text available
N-myristoylation and S-acylation promote protein membrane association, allowing regulation of membrane proteins. However, how widespread this targeting mechanism is in plant signaling processes remains unknown. Through bioinformatics analyses, we determined that among plant protein kinase families, the occurrence of motifs indicative for dual lipid...
Preprint
Full-text available
The phytohormone abscisic acid (ABA) regulates various growth- and developmental processes in response to limiting water conditions. ABA functions through an established signaling pathway consisting of PYR1/PYL/RCAR ABA receptors that inhibit group A type 2C protein phosphatases (PP2Cs) in an ABA-dependent manner. Inhibition of PP2Cs enables the ac...
Article
Ca2+ signals are transient, hence, upon a stimulus-induced increase in cytosolic Ca2+ concentration, cells have to re-establish resting Ca2+ levels. Ca2+ extrusion is operated by a wealth of transporters, such as Ca2+ pumps and Ca2+/H+ antiporters, which often require a rise in Ca2+ concentration to be activated. Here, we report a regulatory fine-t...
Article
Significance Reactive oxygen species (ROS) production is induced by multiple environmental stresses in various organisms. In plants, ROS transduce local and systemic signaling for adaptation and tolerance to these stresses. Here we show that red clover necrotic mosaic virus (RCNMV), a plant positive-strand RNA [(+)RNA] virus, hijacks the host’s ROS...
Article
Full-text available
Living organisms sense and respond to changes in nutrient availability to cope with diverse environmental conditions. Nitrate (NO3 (-)) is the main source of nitrogen for plants and is a major component in fertilizer. Unraveling the molecular basis of nitrate sensing and regulation of nitrate uptake should enable the development of strategies to in...
Article
Full-text available
Ca2+ signatures are central to developmental processes and adaptive responses in plants. However, high-resolution studies of Ca2+ dynamics using genetically encoded Ca2+ indicators (GECIs) such as Yellow Cameleon (YC) proteins have so far not been conducted in important model crops such as rice (Oryza sativa).We conducted a comparative study of 35S...
Article
Under drought stress, abscisic acid (ABA) triggers closure of leaf cell pores called stomata, which are formed by two specialized cells called guard cells in plant epidermis. Two pathways downstream of ABA stimulate phosphorylation of the S-type anion channels SLAC1 (slow anion channel associated 1) and SLAH3 (SLAC1 homolog 3), which causes these c...
Article
Full-text available
Stimulus specific accumulation of second messengers like reactive oxygen species (ROS) and Ca(2+) are central to many signaling and regulation processes in plants. However, mechanisms that govern the reciprocal interrelation of Ca(2+) and ROS signaling are only beginning to emerge. NADPH oxidases of the respiratory burst oxidase homologue (RBOH) fa...
Article
Full-text available
Calcineurin B-like proteins (CBLs) represent a family of calcium sensor proteins that interact with a group of serine/threonine kinases designated as CBL-interacting protein kinases (CIPKs). CBL-CIPK complexes are crucially involved in relaying plant responses to many environmental signals and in regulating ion fluxes. However, the biochemical char...
Article
Temporally and spatially defined calcium signatures are integral parts of numerous signalling pathways. Monitoring calcium dynamics with high spatial and temporal resolution is therefore critically important to understand how this ubiquitous second messenger can control diverse cellular responses. Yellow cameleons (YCs) are fluorescence resonance e...
Article
Ca(2+) is a crucial second messenger that is involved in mediating responses to various biotic and abiotic environmental cues and in the regulation of many developmental processes in plants. Intracellular Ca(2+) signals are realized by spatially and temporally defined changes in Ca(2+) concentration that represent stimulus-specific Ca(2+) signature...
Article
Full-text available
Potassium (K(+)) channel function is fundamental to many physiological processes. However, components and mechanisms regulating the activity of plant K(+) channels remain poorly understood. Here, we show that the calcium (Ca(2+)) sensor CBL4 together with the interacting protein kinase CIPK6 modulates the activity and plasma membrane (PM) targeting...
Article
Fluorescent tagging of proteins and confocal imaging techniques have become methods of choice in analysing the distributions and dynamic characteristics of proteins at the subcellular level. In common use are a number of strategies for transient expression that greatly reduce the preparation time in advance of imaging, but their applications are li...
Article
Ca(2+) signals are core transducers and regulators in many adaptation and developmental processes of plants. Ca(2+) signals are represented by stimulus-specific signatures that result from the concerted action of channels, pumps, and carriers that shape temporally and spatially defined Ca(2+) elevations. Cellular Ca(2+) signals are decoded and tran...
Article
The specificity of intracellular signaling and developmental patterning in biological systems relies on selective interactions between different proteins in specific cellular compartments. The identification of such protein-protein interactions is essential for unraveling complex signaling and regulatory networks. Recently, bimolecular fluorescence...
Article
Full-text available
In conjunction with Ca2� signaling, the subcellular localization of Ca2� channels in various plant cells has been under intense scrutiny. The present study focused on the subcellular localization of OsTPC1, a putative voltage-dependent calcium channel in rice. A newly developed antibody to the linker domain of OsTPC1 was shown to bind this protein...
Article
We isolated a cDNA (OsTPC1) from rice that was homologous to AtTPC1, a putative voltage-dependent Ca(2+) channel (VDCC) gene of Arabidopsis thaliana. The hydropathy profile of its deduced amino acid sequence showed significant structural features of the alpha 1-subunit of animal VDCCs. Functional analysis using a heterologous yeast expression syste...
Article
The electric control of cellular functions via Ca2+ was formerly suggested. From this viewpoint, the involvement of a Ca2+ channel was studied using bovine fetal arterial endothelial (BFAE) cells in which P2X4, an ATP-operated and fluid shear stress sensitive Ca2+ channel, exists predominantly. An electric stimulus (sine wave, 10 Hz, 10 VPP, 30 s)...

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