Kelsey Derricks Sack

Kelsey Derricks Sack
Boston University | BU · Molecular and Translational Medicine

MD/PhD

About

23
Publications
1,465
Reads
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168
Citations
Introduction
Skills and Expertise
Additional affiliations
June 2017 - present
Beth Israel Deaconess Medical Center
Position
  • Internal Medicine Resident
August 2010 - May 2017
Boston University
Position
  • PhD Student
November 2007 - August 2010
Boston University
Position
  • Senior Project/Student Researcher

Publications

Publications (23)
Article
Full-text available
Endothelial dysfunction accompanies the microvascular thrombosis commonly observed in severe COVID-19. Constitutively, the endothelial surface is anticoagulant, a property maintained at least in part via signaling through the Tie2 receptor. During inflammation, the Tie2 antagonist angiopoietin-2 (Angpt-2) is released from endothelial cells and inhi...
Preprint
Profound endothelial dysfunction accompanies the microvascular thrombosis commonly observed in severe COVID-19. In the quiescent state, the endothelial surface is anticoagulant, a property maintained at least in part via constitutive signaling through the Tie2 receptor. During inflammation, the Tie2 antagonist angiopoietin-2 (Angpt-2) is released f...
Article
Background: Optimal management of acute pulmonary embolism requires expertise offered by multiple subspecialties. As such, pulmonary embolism response teams (PERT) have increased in prevalence, but the institutional consequences of a PERT are unclear. Methods: We compared all patients that presented to our institution with an acute pulmonary emb...
Article
Lethal features of sepsis and acute respiratory distress syndrome (ARDS) relate to the health of small blood vessels. For example, alveolar infiltration with proteinaceous fluid is often driven by breach of the microvascular barrier. Spontaneous thrombus formation within inflamed microvessels exacerbates organ ischemia, and in its final stages, eru...
Article
Full-text available
Epithelial wound healing requires the coordination of cells to migrate as a unit over the basement membrane after injury. To understand the process of this coordinated movement, it is critical to study the dynamics of cell-cell communication. We developed a method to characterize the injury-induced sustained Ca²⁺ mobilizations that travel between c...
Data
Association of P2X7 and pannexin1 protein in epithelial cells. HCLE cells were cultured until confluent, and cross-linking was performed with formaldehyde in situ, as previously described [12]. Each crosslinked experimental sample (labeled “CL”) and its corresponding control were heated at two different temperature settings: 65°C (to maintain cross...
Data
Sustained Ca2+ oscillations detected after scratch-wounding. Confluent cells were preincubated with 5 μM of Fluo3-AM for 30 minutes. Cells were scratch-wounded and imaged for 2 hours in an environmental chamber mounted on a Zeiss 880 confocal microscope (10x). Images were taken every 3 seconds, with the movie at 25 fps. Scale bar = 60 μm. (AVI)
Data
Sustained Ca2+ oscillations induced by UTP. Confluent HCLE cells were preincubated with 5μM of Fluo3-AM for 30 minutes. Cells were stimulated with 25 μM UTP and imaged for 45 minutes in an environmental chamber mounted on a Zeiss 880 confocal microscope (20x). Images were taken every 3 seconds, with the movie at 25 fps. Scale Bar = 50 μm. (AVI)
Data
Ca2+ mobilizations in organ culture. Mouse corneas were preincubated with 15 μM Fluo3-AM for 30 minutes and CellMask Deep Red Plasma membrane stain at recommended concentration for 5 minutes. Cells were scratch-wounded and imaged for at least 15 mins in an environmental chamber mounted on a Zeiss 880 confocal microscope with AIRYSCAN Fast Module (2...
Data
Representative kymograph of cells at least 2 cell rows away from the wound edge. Compared to the kymographs made from cells at the wound edge (LE), the Ca2+ response showed less intensity. Brackets on the left and each horizontal line represent activity of a single cell (n = 7). (TIF)
Data
Sustained Ca2+ oscillations induced by BzATP stimulation. Confluent HCLE cells were preincubated with 5 μM of Fluo3-AM for 30 minutes. Cells were stimulated with 25 μM BzATP and imaged for 45 minutes in an environmental chamber mounted on a Zeiss 880 confocal microscope (20x). Images were taken every 3 seconds, with the movie at 25 fps. Scale Bar =...
Data
Pannexin scrambled peptide does not inhibit rate of wound closure. Confluent cells were treated with 100 μM Scrambled Panx control peptide for an hour before being preincubated with 5 μM Fluo3-AM for 30 minutes. Cells were scratch-wounded and imaged for 16 hours in an environmental chamber mounted on a Zeiss 880 confocal microscope (20x). Images we...
Data
Ca2+ mobilizations and cell shape. Confluent HCLE cells were preincubated with 5 μM Fluo3-AM for 30 minutes and CellMask Deep Red Plasma membrane stain at recommended concentration for 5 minutes. Cells were scratch-wounded and imaged for 45 minutes in an environmental chamber mounted on a Zeiss 880 confocal microscope (40x oil). Images were taken e...
Data
10Panx significantly attenuates wound closure rate. Confluent HCLE cells were treated with 100 μM 10Panx inhibitory peptide for an hour before being preincubated with 5 μM Fluo3-AM for 30 minutes. Cells were scratch-wounded and imaged for 16 hours in an environmental chamber mounted on a Zeiss 880 confocal microscope (20x). Images were taken every...
Preprint
Full-text available
Epithelial wound healing requires the coordination of cells to migrate as a unit over the basement membrane after injury. To understand the process of this coordinated movement, it is critical to study the dynamics of cell-cell communication. We developed a method to characterize the injury-induced sustained Ca2+ mobilizations that travel between c...
Article
Full-text available
Vascular endothelial growth factor A (VEGF) drives endothelial cell maintenance and angiogenesis. Endothelial cell behavior is altered by the stiffness of the substrate the cells are attached to suggesting that VEGF activity might be influenced by the mechanical cellular environment. We hypothesized that extracellular matrix (ECM) stiffness modifie...
Article
Full-text available
Vascular disease and its associated complications are the number one cause of death in the Western world. Both extracellular matrix stiffening and dysfunctional endothelial cells contribute to vascular disease. We examined endothelial cell calcium signaling in response to VEGF as a function of extracellular matrix stiffness. We developed a new anal...
Article
Full-text available
The process of wound healing must be tightly regulated to achieve successful restoration of injured tissue. Previously, we demonstrated that when injury to corneal epithelium occurs, nucleotides and neuronal factors are released to the extracellular milieu, generating a Ca(2+) wave from the origin of the wound to neighboring cells. In the present s...
Article
Full-text available
Extracellular matrix remodeling is a continuous process that is critical to maintaining tissue homeostasis, and alterations in this process have been implicated in chronic diseases such as atherosclerosis, lung fibrosis, and emphysema. Collagen and elastin are subject to ascorbate-dependent hydroxylation. While this post-translational modification...
Article
Full-text available
A broad range of cells are subjected to irregular time varying mechanical stimuli within the body, particularly in the respiratory and circulatory systems. Mechanical stretch is an important factor in determining cell function; however, the effects of variable stretch remain unexplored. In order to investigate the effects of variable stretch, we de...

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