Keagile Bati

Keagile Bati
Botswana International University of Science and Technology | BIUST · Biology and Biotechnology

MSc: Biological Sciences and Biotechnology

About

6
Publications
3,491
Reads
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21
Citations
Introduction
Keagile Bati currently works at the Biology and Biotechnology, Botswana International University of Science and Technology. Their most recent publication is 'The Inhibitory Effect of An Ethanol Extract of Sida rhombifolia Leaves on Key Carbohydrate Hydrolyzing Enzymes.'.
Additional affiliations
November 2018 - present
Botswana International University of Science and Technology
Position
  • Research and Teaching Assistant
Description
  • Conducting tutorial sessions and laboratory practical demonstrations for undergraduate classes. Also, conduct experiments like enzyme kinetics, plant extracts efficacy testing, DNA/RNA extraction, protein studies and disease animal models.
July 2017 - July 2018
African Society for Laboratory Medicine, ASLM
Position
  • Research Assistant
Description
  • Assisting in clinical trial of dried blood spot (DBS) test in HIV viral load determination. The project was a partnership between ASLM, Centres for Disease Control and Prevention (CDC) and the Ministry of Health Botswana.
Education
November 2018 - October 2020
Botswana International University of Science and Technology
Field of study
  • Biological Sciences and Biotechnology (specialization: Biochemistry and Molecular Physiology)
August 2012 - May 2017
University of Botswana
Field of study
  • Biological Sciences and Chemistry

Publications

Publications (6)
Article
Full-text available
Several medicinal plants have anti-diabetic properties and can be considered as an alternative therapy because of their actions which are like those of conventional antidiabetic drugs. In this study, we investigated Myrothamnus flabellifolius (MF) ethanol: water (70:30) extract for its possible anti-diabetic potential in streptozotocin-high energy...
Conference Paper
Full-text available
Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia due to absolute or relative insulin deficiency and action. With the continued search for antidiabetic drugs, PPARs agonists have gained attention as target for drug development. Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear receptor sup...
Article
Full-text available
Aim: The study was conducted to screen the ethanol extract of Sida rhombifolia (ESR) leaves for its phytochemical constituents and elucidate some of its possible mechanism of action in lowering hyperglycemia. Methodology: The ethanol ESR was prepared and its effect on carbohydrate hydrolyzing enzymes (α-amylase and α-glucosidase) both in vitro and...
Article
Full-text available
Objectives This study aimed to evaluate the hypoglycemic effects of an ethanol extract of Cassia abbreviata (ECA) bark and the possible mechanisms of its action in diabetic albino rats. Methods ECA was prepared by soaking the powdered plant material in 70% ethanol. It was filtered and made solvent-free by evaporation on a rotary evaporator. Type 2...
Article
Full-text available
Contamination of vegetables with toxic metals is one of the most important contributing factors to ill health throughout the world, more so because vegetables are considered essential for human health and their consumption is highly recommended by health authorities. The aim of this study was to determine the concentrations of selected essential el...

Questions

Questions (5)
Question
Alpha glucosidases are implicated in the management of postprandial glycemia in type 2 diabetic subjects, however, in most cases we use the crude acetone powder of the intestines or crude from fresh rat intestines. I therefore wish to isolate and purify the enzymes like sucrase and maltase from the crude rat intestines, therefore i will appreciate any protocol or paper that has done the same or anything related to that.
Thank you!
Question
When conducting OGTT to determine the potential hypoglycemic effect of a drug, which is more scientifically correct?
A. Giving the animal the drug first then glucose solution latter. OR
B. Giving the animal glucose solution first and then the drug latter. OR
C. Combing the drug and glucose solution and giving the animal one time?

Network

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Projects

Projects (2)
Project
Euclea natalensis is used traditionally for the management of diabetes, therefore, our project aims to explore and determine its efficacy on diabetic rats induced by a high energy diet and low dose Streptozotozin. The project entails experiments such as oral glucose tolerance test (OGTT), Glucose uptake studies, enzyme inhibition and kinetics, cytotoxicity and genotoxicity studies, westernblot studies for GLUT4 protein and RT-PCR for gene expression analysis. Phytochemistry and isolation and identification of bioactive compounds will also be conducted and lastly molecular docking and molecular dynamics studies done.
Archived project
The study seeks to compile a scientific evidence on the status of heavy metal contamination of the Environmental spheres (air, water, soil, sediments) and the Biota (plants and animals) in Botswana for the period 2000 - 2017. The review will serve as guide to policy making and research for both academics and public health. Public health risk due to exposure to heavy metals will be computed based on daily metal intake and target hazard quotient.