Katrine BaumannUniversity of Copenhagen · LEO Foundation Skin Immunology Research Center
Katrine Baumann
PhD
Postdoctoral Fellow at the BRIDGE - Translational Excellence Programme, University of Copenhagen || RefLab ApS
About
20
Publications
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Introduction
Skills and Expertise
Publications
Publications (20)
Introduction
Because MRGPRX2 is now recognized as the mast cell receptor for basic secretagogues, there is currently a tremendous interest in whether MRGRPX2 could play an important role in various pruritic dermatoses such as chronic spontaneous urticaria. Therefore, we sought to identify new potent and selective antagonists to pharmacologically ch...
Background
Patients suffering from chronic spontaneous urticaria (CSU) are typically classified as type I or type IIb autoimmune CSU, but further patient stratification is hindered by the lack of biomarkers.
Objectives
We investigated whether the histamine content of individual basophils differ between patient subtypes in CSU to evaluate its poten...
Introduction:
Detection of specific food allergens brought into circulation following ingestion is complicated by the minute amounts present in serum. We therefore aimed to assess the utility of selective removal of IgE against peanut components using ImmunoCAP combined with the basophil histamine release (HR) assay (BHRA) to identify the absorbed...
Skin-barrier restoration following abrasive trauma is facilitated by mediator release from skin-resident cells, a process that has been investigated primarily in mice or simplified human systems with previous studies focusing on a limited number of biomarkers. Here, we demonstrate how early events caused by skin-barrier disruption can be studied in...
Background
Pituitary adenylate cyclase-activating polypeptide-38 (PACAP38) and vasoactive intestinal polypeptide can provoke cluster headache attacks in up to half of cluster headache patients in their active phase. At present, it is unknown whether provoked attacks are mediated via calcitonin gene-related peptide or mast cell activation.
Methods...
Allergy to galactose‐1,3‐alpha‐galactose (alpha‐gal) is a rare IgE‐mediated allergy1, where symptoms mainly occur hours2,3 after ingestion of mammalian innards or meat, e.g. pork, beef, and lamb. Knowledge on absorption rate and size of allergens, i.e. proteins and especially glycoproteins and glycolipids, are sparse, with many variables involved;...
Basophil testing is the most effective single approach for diagnosing type-IIb autoimmune chronic spontaneous urticaria (TIIbaiCSU). A positive basophil test has been linked to long disease duration, higher disease activity, a poor response to antihistamines and omalizumab, and a better response to cyclosporine and fenebrutinib. As of now it is unc...
Severe reactions in IgE‐mediated food‐allergic patients can occur within minutes after ingestion, but the eliciting mechanism(s), linking the amount of allergenic food to the heterogeneity of clinical manifestations, are yet unknown. Quantification of IgE reactive allergenic food in circulation is a prerequisite for understanding the kinetics of th...
Background
Autoimmune chronic spontaneous urticaria (CSU) is due to mast cell (MC)‐activating autoantibodies, which are screened for by the autologous serum skin test (ASST) and basophil tests (BTs). Many CSU patients are positive in only one of these tests. How often this occurs and why is currently unknown.
Objectives
To characterize the prevale...
In development of peptide therapeutics, rodents are commonly-used preclinical models when screening compounds for efficacy endpoints in the early stages of discovery projects. During the screening process, some peptides administered subcutaneously to rodents caused injection site reactions manifesting as localized swelling. Screening by postmortem...
Basophils and mast cells are known for their capability to release both preformed and newly synthesized inflammatory mediators. In this chapter, we describe how to stimulate and detect histamine released from basophils in whole blood, purified basophils, in vitro cultured mast cells, and in situ skin mast cells (the latter by microdialysis), using...
Microdialysis is a well-established technique for sampling of small molecules from the human skin, but larger molecules are more difficult to recover. Consequently, sampling feasibility must be evaluated before microdialysis is used in vivo. This report presents a tool for estimating the recovery of large biomarkers from human skin by microdialysis...
Abstract Skin microdialysis (SMD) is a versatile sampling technique that can be used to recover soluble endogenous and exogenous molecules from the extracellular compartment of human skin. Due to its minimally invasive character, SMD can be applied in both clinical and preclinical settings. Despite being available since the 1990s, the technique has...
Treatment with the TRPV1 agonist, capsaicin, was previously shown to protect against experimental colitis in the severe combined immunodeficiency (SCID) T-cell transfer model. Here, we investigate trpv1 gene expression in lymphoid organs and cells from SCID and BALB/c mice to identify a potential target for the anti-inflammatory effect of capsaicin...
Questions
Questions (3)
Does anyone have any experience regarding reversing the effect of EDTA in whole blood samples (e.g. by addition of calcium ions) prior to basophil activation studies?
Currently, we are washing the blood three times with buffer to get rid of the EDTA, but it would be much easier if we could just add ions to reverse the chelating effects.
Thanks!
I am using OPD tablets from Sigma as a substrate for my standard sandwich ELISA kits with HRP. I stop the reaction using 3M H2SO4 as described in the data sheet and the plate is then measured at 492 nm accordingly. This far I have used a microplate reader with a 630 nm filter as a reference wavelength and subtracted these OD values from the ones obtained at 492 nm. But now I am only able to access an instrument with a 600 nm filter instead of 630 nm. Is that sufficient or should I rather buy a new filter or not use any correction at all?
I am currently doing some studies in which I incubate PBMCs from 4 different donors with a concentration range of a drug for 3 different incubation times.
In other words: PBMCs are purified, 6 different concentrations of the drug (0 / 0.01 / 0.03 / 0.1 / 0.3 / 1 ng/ml) are added and the cells are incubated with the drug for either 3, 24 or 48 hours before I measure cytokine levels.
I would like to test whether the concentration of the drug plays a significant role in the cytokine response and at the same time I would like to know whether the amount of incubation time is a significant factor as well.
Which statistical test should I use? I have considered using a two-way repeated measurement ANOVA but I am not sure whether this makes any sense? I guess both time and concentration would be independent variables whereas the cytokine concentration would be the dependent variable?
I would really appreciate some help, since my statistics skills are really lousy.
