Katja BezekUniversity of Primorska | UP · Faculty of Health Sciences (FVZ)
Katja Bezek
Microbiology, PhD
About
16
Publications
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85
Citations
Citations since 2017
Introduction
Additional affiliations
April 2014 - present
December 2012 - March 2014
Publications
Publications (16)
Introduction
The composition of the human gut microbiota, which can also be regulated by the consumption of probiotics, has a significant impact on host health. The main source of probiotics can be foods such as fermented foods, yogurts, fermented drinks and/or probiotic supplements. While parents play a critical role in ensuring the well-being of...
In an 8-week double-blind study with two parallel groups in which eligible healthy participants were randomly assigned to H. erinaceus supplement group (N=18; 67% F, 33% M), and placebo group (N=14; 67% F, 33% M). A food supplement produced from H. erinaceus fruiting bodies by MycoMedica d.o.o., Slovenia was used in this trial with a 3,44 mg daily...
(1) Background: According to the emergence and spread of antibiotic resistance, there is an urge for new promising substances. The purpose of the study was to test the antioxidant, cytotoxic and antimicrobial properties of the Helichrysum italicum (Roth) G. Don essential oil (EO) and hydrosol. (2) Methods: The antioxidant potential was determined u...
Campylobacter jejuni is an emerging food-borne pathogen that poses a high risk to human health. Knowledge of the strain source can contribute significantly to an understanding of this pathogen, and can lead to improved control measures in the food-processing industry. In this study, slaughterhouse and surface-water isolates of C. jejuni were charac...
The dynamics and diversity of human gut microbiota that can remarkably influence the wellbeing and health of the host are constantly changing through the host's lifetime in response to various factors. The aim of the present study was to determine a set of parameters that could have a major impact on classifying subjects into a single cluster regar...
Here we present a research of bacterial adhesion to two most often used materials in modern kitchens, namely food grade ceramics and Teflon. To test the bacterial adhesion on kitchen worktops Escherichia coli, Pseudomonas aeruginosa and Campylobacter jejuni were used as the most common foodborne contaminants. Contact angle, roughness and streaming...
The new doctoral study Prevention for Health is a modern and innovative study programme in which we aim to develop holistic preventive approaches to the most important public health problems in today's society in Slovenia and around the world. The study combines the fields of kinesiology, nursing, dietetics and physiotherapy in accordance with scie...
Bacterial adhesion to surfaces is a prerequisite for formation of biofilms – multicellular communities embedded in an extracellular medium. Biofilms hamper the removal of bacteria and contribute to their resistance to antibiotics, thus representing a serious problem in medicine and industry. Bacterial adhesion is therefore an important factor in pr...
There is a wide range of factors affecting bacterial adhesion and biofilm formation. However, in both food processing and medical settings, it is very hard to obtain suitably controlled conditions so that the factors that reduce surface colonisation and biofouling can be studied. The aim of this study was to evaluate the effect of glucose concentra...
Thermophilic campylobacters are a major cause of bacterial food-borne diarrhoeal disease. Adherence and biofilm formation are key elements of Campylobacter jejuni persistence in unfavourable environmental conditions. The phytochemical analysis of Euodia ruticarpa fruit ethanol solution extract (EREE) indicated that the major compounds were evodiami...
Questions
Questions (6)
Any references of inducing VBNC state of Staphylococcus aureus and its detection in a time shorter than 30 days?
Thank you!
Has advanced DMEM, supplemented with Glutamax and 5% FBS any effect on the growth of Campylobacter jejuni? What concentration of Gentamicine is usually used for gentamicin adhesion/invasion assay?
Is anyone familiar with the use of PrestoBlue® Cell Viability Reagent for mimum inhibitory concentrationson determination in the case of Campylobacter (bacteria)? Is it suitable?
I need a quickly method for biofilm screening assay for 50 C. jejuni isolates. I started with crystal violet but there are so many critical points of the method and low reproducibility. Does anybody have data for crystal violet method that has worked well?
I would like to have information about; how to prepare inoculum (medium, incubation time); the growth medium for biofilm formation (volumes, incubation time); washing of unbounded cells (medium, volume, how many times); fixation of cells (drying…); staining (volume of CV, %, time); washing (medium, volume, how many times); drying; destaining (solution, %, time); wavelength for absorbance measurement)... Thank you!
I have a question about calculating the Specific Biofilm Formation index (SBF= AB-CW)/G) (Niu and Gilbert, 2004; Naves et al., 2008). Which value of cell suspension after incubation time to use!? If we have culture in 96 well plate, should we measure the optical density of suspension in the same micro titer plate as it was incubated or should we transfer the cell suspension to sterile micro titer plate and measure optical density? And which wave light is the most appropriate?
