• Home
  • Katharina Schaufler
Katharina Schaufler

Katharina Schaufler
Kiel University and University of Greifswald

Professor
Group leader at University of Greifswald and Professor at Kiel University

About

57
Publications
9,325
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,040
Citations
Introduction
My workgroup addresses factors that contribute to the success of antibiotic-resistant bacteria. This does not only include the characterization of resistance but also the investigation of virulence and fitness determinants. With geno- and phenotypic experiments including experimental evolution, functional and phylogenetic genome analysis as well as biofilm formation assays it is our main goal to analyze, to better understand and to fight successful pandemic pathogens. In collaboration with others, we elucidate the occurrence and transmission of antibiotic-resistant bacteria in humans, animals (including animal products) and the environment in a One Health context and push towards identifying innovative bacterial therapeutic targets.
Additional affiliations
November 2020 - present
University of Greifswald
Position
  • Group Leader
January 2019 - October 2020
University of Greifswald
Position
  • Professor
September 2018 - December 2018
University of Greifswald
Position
  • PostDoc Position

Publications

Publications (57)
Article
Screening for biofilm inhibition by purified natural compounds is difficult due to compounds’ chemical diversity and limited commercial availability, combined with time- and cost-intensiveness of the laboratory process. In silico prediction of chemical and biological properties of molecules is a widely used technique when experimental data availabi...
Article
Full-text available
Studies have previously described the occurrence of multidrug-resistant (MDR) Escherichia coli in human and veterinary medical settings, livestock, and, to a lesser extent, in the environment and food. While they mostly analyzed foodborne E. coli regarding phenotypic and sometimes genotypic antibiotic resistance and basic phylogenetic classificatio...
Preprint
Full-text available
Studies have previously described the occurrence of multidrug-resistant (MDR) Escherichia coli in human and veterinary medical settings, livestock, and, to a lesser extent, in the environment and food. While they mostly analyzed foodborne E. coli regarding phenotypic and sometimes genotypic antibiotic resistance and basic phylogenetic classificatio...
Article
Full-text available
The ability of extensively drug-resistant (XDR) Klebsiella pneumoniae to rapidly acquire resistance to novel antibiotics is a global concern. Moreover, Klebsiella clonal lineages that successfully combine resistance and hypervirulence have increasingly occurred during the last years. However, the underlying mechanisms of counteracting fitness costs...
Article
Full-text available
Antimicrobial resistance is an increasing global problem and complicates successful treatments of bacterial infections in animals and humans. We conducted a longitudinal study in Meck‐lenburg‐Western Pomerania to compare the occurrence of ESBL‐producing Escherichia (E.) coli in three conventional and four organic pig farms. ESBL‐ positive E. coli,...
Article
Full-text available
Antimicrobial resistance (AMR) is a serious global health threat and extended-spectrum beta-lactamase (ESBL)-producing Enterobacterales are a major contributor. This study aimed to gain a deeper insight into the AMR burden of wild animals. In total, 1595 fecal samples were collected by two systematic searches in Mecklenburg-Western Pomerania, north...
Article
Full-text available
Significance Carbapenem-resistant Klebsiella pneumoniae belongs to the “critical-priority” tier of bacterial pathogens as identified by the World Health Organization. Emerging “high-risk” lineages are responsible for difficult-to-treat, hospital-acquired infections and outbreaks around the globe. By integrating genomic and epidemiological data for...
Article
Full-text available
The objectives of this study were to ascertain the fecal ESBL/AmpC-E. coli prevalence and to detect risk factors for their occurrence in young pre-weaned calves and their dams on large dairy farms in Germany. From 2018–2019 we investigated 2816 individual fecal samples from pre-weaned dairy calves and their dams, representing seventy-two farms (mea...
Article
Full-text available
Background: Klebsiella pneumoniae causes severe diseases including sepsis, pneumonia and wound infections and is differentiated into hypervirulent (hvKp) and classic (cKp) pathotypes. hvKp isolates are characterized clinically by invasive and multiple site infection and phenotypically in particular through hypermucoviscosity and increased sideroph...
Article
Almost one-third of all proteins require metal ions as an essential component in key biological processes and approximately half of all enzymes are associated with one or more metal ions. The naturally occurring selenium is very toxic at higher levels, but few bacteria can reduce it into the less toxic insoluble elemental selenium. Selenium is requ...
Article
Aim: To verify synergistic effects we investigated the antimicrobial activity of seven phenolic phytochemicals (gallic acid; epicatechin; epigallocatechin gallate; daidzein; genistein; myricetin; 3-hydroxy-6-methoxyflavone) in combination with six antibiotics against multidrug-resistant isolates from the ESKAPE group. Methods and results: To inv...
Article
Full-text available
The emergence of carbapenemase-producing Enterobacteriaceae limits therapeutic options and presents a major public health problem. Resistances to carbapenems are mostly conveyed by metallo-beta-lactamases (MBL) including VIM, which are often encoded on resistance plasmids. We characterized four VIM-positive isolates that were obtained as part of a...
Preprint
Full-text available
A protracted outbreak of New Delhi metallo-beta-lactamase (NDM)-producing carbapenem-resistant Klebsiella pneumoniae , started in Tuscany, Italy, in November 2018 and continued in 2020 and through 2021. To understand the regional emergence and transmission dynamics over time, we collected and sequenced the genomes of 117 extensively drug-resistant,...
Article
Beta glucans are complex glucose polymers well known for their immune modulatory properties. Therefore they are used and advertised in dietary supplements. Unfortunately there is no standardized test system for quality control of such health-related foods. This approach combined wet chemical and enzyme-based quantification methods (e.g. aniline blu...
Article
Full-text available
Minimal inhibitory concentration-based pharmacokinetic/pharmacodynamic (PK/PD) indices are commonly applied to antibiotic dosing optimisation, but their informative value is limited, as they do not account for bacterial growth dynamics over time. We aimed to comprehensively characterise the exposure–effect relationship of levofloxacin against Esche...
Article
Full-text available
Antibiotic-resistant Enterobacteriaceae are regularly detected in livestock. As pathogens, they cause difficult-to-treat infections and, as commensals, they may serve as a source of resistance genes for other bacteria. Slaughterhouses produce significant amounts of wastewater containing antimicrobial-resistant bacteria (AMRB), which are released in...
Article
Full-text available
Multi-drug resistant (MDR), gram-negative Enterobacteriaceae, such as Escherichia coli (E. coli) limit therapeutic options and increase morbidity, mortality, and treatment costs worldwide. They pose a serious burden on healthcare systems, especially in developing countries like Rwanda. Several studies have shown the effects caused by the global spr...
Article
Full-text available
To increase our understanding of bacterial intestinal colonization in animal populations lacking substantial anthropogenic influence we studied the diversity of E. coli in cormorants from the pristine West-Mongolian steppe. E. coli were isolated from individual birds of two cormorant colonies located on small islands in lakes at least 100 km away f...
Article
Aim An outbreak of the extensively drug-resistant (XDR) K. pneumoniae strain ST307 in a cluster of North-German hospitals gave rise to the assumption that the epidemiological success of the strain could be based on an increased tolerance to biocides. Methods The tolerance of the outbreak strain was compared to epidemiologically unrelated clinical...
Article
Full-text available
Background Antibiotic-resistant Klebsiella pneumoniae are a major cause of hospital- and community-acquired infections, including sepsis, liver abscess, and pneumonia, driven mainly by the emergence of successful high-risk clonal lineages. The K. pneumoniae sequence type (ST) 307 lineage has appeared in several different parts of the world after fi...
Article
Full-text available
Abstract Multidrug-resistant gram-negative (MRGN) bacteria are a serious threat to global health. We used genomics to study MRGN obtained from houseflies in a tertiary Rwandan hospital. Our analysis revealed a high abundance of different MRGN including E. coli pathogenic lineage ST131 suggesting the important role of flies in disseminating highly v...
Article
Full-text available
In vitro antiproliferative activities of the 80% methanol extracts of 21 traditionally used Ethiopian medicinal plants were studied using human acute myeloid leukemia (MV4-11) cell line. Among the tested plants, Euphorbia schimperiana Scheele and Gnidia involucrata Steud. ex A. Rich. exhibited significant antiproliferative activity with GI50 values...
Article
Full-text available
Background In addition to the broad dissemination of pathogenic extended-spectrum beta-lactamase (ESBL)-producing Escherichia (E.) coli in human and veterinary medicine and the community, their occurrence in wildlife and the environment is a growing concern. Wild birds in particular often carry clinically relevant ESBL-producing E. coli.Objectives...
Article
Full-text available
Cattle harbor Shiga toxin-producing Escherichia coli (STEC) in their intestinal tract, thereby providing these microorganisms with an ecological niche, but without this colonization leading to any clinical signs. In a preceding study, genotypic characterization of bovine STEC isolates unveiled that their ability to colonize cattle persistently (STE...
Article
Full-text available
Airborne bacteria are a general problem in medical or health care facilities with a high risk for nosocomial infections. Rooms with a continuous airflow, such as operation theaters, are of particular importance due to a possible dissemination and circulation of pathogens including multidrug‐resistant microorganisms. In this regard, a cold atmospher...
Poster
Escherichia coli colonization and infection results in formation of adhesion patterns which in turn contribute to pathotyping of intestinal E. coli. The adhesion patterns of 282 E. coli isolates on different cell lines were determined to establish various adhesion patterns such as diffusely distributed, microcolonies, chains and clumps. Only two st...
Article
From June to October 2019, 17 patients (six infected, 11 colonised) with an extensively drug-resistant (XDR) Klebsiella pneumoniae strain were notified from four Western Pomerania medical facilities. The XDR K. pneumoniae produced carbapenemases NDM-1 and OXA-48, and was only susceptible to chloramphenicol, tigecycline and cefiderocol. Synergistic...
Article
Full-text available
The pathogenic ESBL-producing E. coli lineage ST648 is increasingly reported from multiple origins. Our study of a large and global ST648 collection from multiple hosts (87 whole-genome sequences) combining core and accessory genomics with functional analyses and in vivo experiments suggests that ST648 is a nascent and generalist lineage, lacking c...
Article
Full-text available
Background Extended spectrum beta lactamase (ESBL)-producing extraintestinal pathogenic Escherichia coli infections are of global interest because of their clinical and economic impact. The ESBL resistance genes disseminate through plasmids, and are found in successful global lineages such as ST131 and ST648. The carriage of plasmids has been sugge...
Article
Objectives: Vancomycin-resistant Enterococcus faecium is a leading cause of MDR hospital infection. Two genetically definable populations of E. faecium have been identified: hospital-adapted MDR isolates (clade A) and vancomycin-susceptible commensal strains (clade B). VanN-type vancomycin resistance was identified in two isolates of E. faecium re...
Article
Full-text available
High-risk ESBL-producing Enterobacteriaceae (ESBL-E) have been described in wild birds and rodents worldwide. Rats are of special interest not only due to their indicator role for environmental pollution with multi-resistant bacteria but also as possible infection source. Data on the presence of high-risk ESBL-E in urban wildlife from Africa remain...
Article
Full-text available
The increased presence of clinically relevant multidrug resistant bacteria in natural environments is an emerging challenge for global health care. Little is known regarding the occurrence of extended-spectrum beta-lactamase producing Escherichia coli (ESBL-E. coli) from environmental sentinels in Pakistan. The goal of the current study was to gain...
Article
Full-text available
Objectives: ESBL genes in Escherichia coli are mainly plasmid encoded, although recent studies have also shown chromosomal integration, e.g. in clinical E. coli isolates of ST38. As ESBL-producing E. coli are also found in non-clinical settings, we were interested in determining whether chromosomally integrated ESBL genes occur in ST38 isolates fr...
Article
Full-text available
Biolog Phenotype Microarray (PM) is a technology allowing simultaneous screening of the metabolic behaviour of bacteria under a large number of different conditions. Bacteria may often undergo several cycles of metabolic activity during a Biolog experiment. We introduce a novel algorithm to identify these metabolic cycles in PM experimental data, t...
Data
Sample data. Biolog metabolic signals from E. coli IMT17887, PCV17887 and T17887 tested on plate PM1. Three biological replicates per strain. (ZIP)
Article
Full-text available
The use of whole-genome phylogenetic analysis has revolutionized our understanding of the evolution and spread of many important bacterial pathogens due to the high resolution view it provides. However, the majority of such analyses do not consider the potential role of accessory genes when inferring evolutionary trajectories. Moreover, the recentl...
Article
Plasmid-mediated colistin resistance encoded by themcr-1gene was first described in Enterobacteriaceae isolated from animals, raw meat and humans in China in November 2015[1]. Escherichia coli carrying a transferable colistin resistance plasmid have been reported from different host species and geographic areas. Here we report the first detection o...
Conference Paper
Background: Clinically relevant extended-spectrum beta-lactamase (ESBL)-producing multi-resistant E. coli have been on the rise for years. While, their occurrence was initially restricted to solely a human and veterinary clinical context, recent findings prove their prevalence in extra-clinical settings such as communities, the environment and wild...
Article
Full-text available
Pathogenic ESBL-producing E. coli lineages occur frequently worldwide, not only in a human health context but in animals and the environment, also in settings with low antimicrobial pressures. This study investigated the fitness costs of ESBL-plasmids and their influence on chromosomally encoded features associated with virulence, such as those inv...
Article
Background: International travel contributes to the spread of multidrug-resistant microorganisms including extended spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE). We assessed the proportion of faecal carriers of ESBL-PE among 211 patients with gastrointestinal symptoms who returned to Berlin, Germany, after international travel....
Article
Clinically relevant ESBL-producing multi-resistant Escherichia coli have been on the rise for years. Initially restricted to mostly a clinical context, recent findings prove their prevalence in extra-clinical settings independent of the original occurrence of antimicrobial resistance in the environment. To get further insights into the complex ecol...
Conference Paper
Introduction: Emergence of extended-spectrum beta-lactamase (ESBL)-producing E. coli in wildlife has been recently reported in several parts of the world. It has been hypothesis that wildlife might become infection sources or even reservoirs, contributing to the zoonotic spread of these bacteria. Objectives: With focus on environmental disseminatio...
Article
Full-text available
To contribute to the understanding of multiresistant bacteria, a 'One Health' approach in estimating the rate of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and getting insights into the transmission from clinical settings to the surrounding environment was employed by collecting fecal samples of dogs in a public area. Isolat...
Article
We report the population structure and dynamics of one E. coli population of wild mallard ducks in their natural environment over four winter seasons, following the characterization of 100 isolates each consecutive season. Macrorestriction analysis was used to define isolates variously as multi- or one-year PFGE types. Isolates were characterized g...
Article
Full-text available
To discern the relevance of ST648 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli as a putative new group of multiresistant and extraintestinal pathogenic strains in animals, its frequency, ESBL types, antimicrobial resistance patterns and virulence gene (VG) profiles should be determined and compared with ST131 strains from the sam...
Article
Full-text available
Plasmid-encoded extended-spectrum beta-lactamase (ESBL)-enzymes are frequently produced by Escherichia coli. Several ESBL-plasmids contain genes for toxin-antitoxin (TA) systems, which assure the maintenance of plasmids in bacteria and prevent the cells from "post-segregational killing". These systems limit options to "cure" plasmids of ESBL-wild-t...
Article
Full-text available
Brown rats, Rattus norvegicus, are synanthropic and inhabit urban infrastructures.…

Network

Cited By

Projects

Projects (2)
Project
The aim of this research project is to identify and investigate success features of pandemic, antibiotic-resistant Escherichia coli and Klebsiella pneumoniae in order to develop innovative anti-infectives strategies. By using a wide range of methods that include bioinformatics, microbiology and pharmaceutical biology, we plan transfer our basic research directly into application. In cooperation with internal and external experts and the industry we expect to identify bacterial targets for alternative anti-infectives and to find suitable active compounds. This approach aims to disarm and thus fight infectious, gram-negative pathogens. The spread of these germs is one of the biggest challenges to modern global health.
Project
KEAnI focuses on the development of a technology for the rapid establishment of an antibiogram for nosocomial infections. In cooperation with two SMEs (MEDIPAN GmbH and GA Generic Assays GmbH), the University Hospital Greifswald and the Brandenburg University of Technology Cottbus-Senftenberg, a rapid antibiotic resistance test for sepsis patients will be developed in this project. This enables the direct transfer of research knowledge into therapeutic and economic utilization and application on patients.