Katharina Landfester

Katharina Landfester
Max Planck Institute for Polymer Research | MPIP · Physical Chemistry of Polymers Group

Prof. Dr.

About

900
Publications
123,550
Reads
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39,240
Citations
Additional affiliations
October 2008 - present
Max-Planck-Institut für Polymerforschung
September 2003 - September 2008
Ulm University
January 1999 - September 2003
Max-Planck-Institut für Kolloid- und Grenzflächenforschung

Publications

Publications (900)
Article
The composition of the protein corona varies depending on several parameters and influences the cellular fate of the nanocarriers. Here, we investigated the influence of three key parameters (surface charge, temperature, and plasma concentration) on the formation and composition of the protein corona of polystyrene nanoparticles and ultimately on t...
Article
Filiform corrosion (FFC) is a degradation affecting coated steel in atmospheric conditions. For industrial application the corrosion detection at the early stage is important to apply the reliable protection. Investigation on the use of nanocontainers for the detection and protection of organic layer is described. Fluorescent dye was used to detect...
Article
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Temperature‐responsive polymers display changes of conformation upon a certain threshold temperature, which involves changes of hydrophobicity. Therefore, this property can be exploited to construct materials showing temperature‐dependent interactions with proteins and cells. Indeed, the amounts and types of proteins in the protein corona after inc...
Article
Nanocapsules are an excellent platform for the delivery of macromolecular payloads such as proteins, nucleic acids or polyprodrugs, since they can both protect the sensitive cargo and target its delivery to the desired site of action. However, the release of macromolecules from nanocapsules remains a challenge due to their restricted diffusion thro...
Article
Triggering the release of encapsulated cargos using mechanical stress acting on a nanocarrier is a strategy with potential applications from drug delivery to self-healing coatings. The mechanically triggered release of encapsulated molecules can be controlled by tuning the mechanical properties of the nanocapsules, which are strongly linked to the...
Article
The nucleic acids staining ability of a series asymmetric monomeric monomethine cyanine dyes prepared by simple and reliable synthetic procedure is demonstrated. The dyes show ratiometric red shift of their longer wavelength absorption maxima. It is in the range 514-522 nm neat in TE buffer, 516-528 nm in the presence of DNA, and 518-524 nm in the...
Article
Water-soluble low molecular weight drugs, such as the synthetic glucocorticoid dexamethasone (DXM), can easily leak out of nanocarriers after encapsulation due to their hydrophilic nature and small size. This can lead to a reduced therapeutic efficacy and therefore to unwanted adverse effects on healthy tissue. Targeting DXM to inflammatory cells o...
Article
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Penetration and emanation of light into tissue are limited by the strong interaction of light with the tissue components, especially oxygenated hemoglobin and white adipose tissue. This limits the possibilities for all-optical minimal invasive sensing. In order to minimize the optical losses of light in and out of the tissue, only a narrow optical...
Preprint
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The major challenge of nanocarrier-based anti-cancer vaccination approaches is the targeted delivery of antigens and immunostimulatory agents to cells of interest, such as specific subtypes of dendritic cells (DCs), in order to induce robust antigen-specific anti-tumor responses. An undirected cell and body distribution of nanocarriers can lead to...
Article
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Dibenzo[hi,st]ovalene (DBOV) has excellent photophysical properties, including strong fluorescence and high ambient stability. Moreover, the optical blinking properties of DBOV have enabled optical super-resolution single-molecule localization microscopy with an imaging resolution beyond the diffraction limit. Various organic and inorganic fluoresc...
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The creation of synthetic polymer nanoobjects with well-defined hierarchical structures is important for a wide range of applications such as nanomaterial synthesis, catalysis, and therapeutics. Inspired by the programmability and precise three-dimensional architectures of biomolecules, here we demonstrate the strategy of fabricating controlled hie...
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Responsive nanogel systems are interesting for the drug delivery of bioactive molecules due to their high stability in aqueous media. The development of nanogels that are able to respond to biochemical cues and compatible with the encapsulation and the release of large and sensitive payloads remains challenging. Here, multistimuli-responsive nanoge...
Article
p class="Abstract"> We describe here a method to synthesize ultrasmall nanocapsules with a diameter of 6 nm, exhibiting a well-defined core-shell morphology. Remarkably, the nanocapules are synthesized in a miniemulsion process without the need of large amounts of surfactant as commonly used in the microemulsion process. Ultrasmall nanocapsules wit...
Article
Antibody-modified drug delivery systems in the nano-range have the ability to overcome current challenges for treating diseases due to their high specificity towards the targeted body region. However, no antibody-bound nanocarrier has been clinically approved to date. This missing clinical approval may be a result of the conjugation strategy that i...
Article
Polymer vesicles that mimic the function of cell membranes can be obtained through the self-assembly of amphiphilic block copolymers. The cell-like characteristics of polymer vesicles, such as the core-shell structure, semi-permeability and tunable surface chemistry make them excellent building blocks for artificial cells. However, the standard pre...
Article
N‐heterocyclic compounds demonstrate wide applications ranging from natural compound production to coordination chemistry. Usually, the synthesis of N‐heterocyclic compounds is conducted under thermal conditions, mostly by Lewis acids or metal‐containing compounds as molecular catalysts. Here, we report a photocatalytic route for aryl azoline forma...
Article
The current spraying of agrochemicals is unselective and ineffective, consuming a high amount of fungicides, which endangers the environment and human health. Cellulose-based nanocarriers (NCs) are a promising tool in sustainable agriculture and suitable vehicles for stimuli-responsive release of agrochemicals to target cellulase-segregating fungi,...
Article
We describe here a method to synthesize ultrasmall nanocapsules with a diameter of 6 nm, exhibiting a well-defined core-shell morphology. Remarkably, the nanocapules are synthesized in a miniemulsion process without the need of large amounts of surfactant as commonly used in the microemulsion process. Ultrasmall nanocapsules with an oil core and a...
Article
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Polymers containing sulfur centers with high oxidation states in the main chain, polysulfoxide and polysulfone, display desirable properties such as thermomechanical and chemical stability. To circumvent their challenging direct synthesis, methods based on the oxidation of a parent polysulfide have been developed but are plagued by uncontrolled rea...
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Cellular, inter‐organismal and cross kingdom communication via extracellular vesicles (EVs) is intensively studied in basic science with high expectation for a large variety of bio‐technological applications. EVs intrinsically possess many attributes of a drug delivery vehicle. Beyond the implications for basic cell biology, academic and industrial...
Article
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Spraying of agrochemicals (pesticides, fertilizers) causes environmental pollution on a million‐ton scale. A sustainable alternative is target‐specific, on‐demand drug delivery by polymeric nanocarriers. Trunk injections of aqueous nanocarrier dispersions can overcome the biological size barriers of roots and leaves and allow distributing the nanoc...
Article
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Polymeric vesicles are excellent building blocks of synthetic compartmentalized systems such as protocells and artificial organelles. In such applications, the efficient encapsulation of materials into the vesicles is an essential requirement. However, common encapsulation techniques can be time-consuming, demand special equipment or have limited e...
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Optical imaging probes have played a major role in detecting and monitoring a variety of diseases. In particular, nonlinear optical imaging probes, such as second harmonic generating (SHG) nanoprobes, hold great promise as clinical contrast agents, as they can be imaged with little background signal and unmatched long-term photostability. As their...
Article
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Supraparticles are large clusters of much smaller colloidal particles. Controlling the shape and anisotropy of supraparticles can enhance their functionality, enabling applications in fields such as optics, magnetics, and medicine. The evaporation of self-lubricating colloidal ouzo droplets is an easy and efficient strategy to create supraparticles...
Article
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For nanocarriers with low protein affinity, we show that the interaction of nanocarriers with cells is mainly affected by the density, the molecular weight, and the conformation of polyethylene glycol (PEG) chains bound to the nanocarrier surface. We achieve a reduction of nonspecific uptake of ovalbumin nanocarriers by dendritic cells using densel...
Article
Nanoreactors offer a great platform for the onsite generation of functional products. However, the production of the desired compound is often limited by either the availability of the reagents or their diffusion across the nanoreactor shell. To overcome this issue, we synthesized self-sustaining nanoreactors carrying the required reagents with the...
Article
Hair follicles (HFs) are important drug delivery targets for the therapy of miscellaneous skin diseases and for skin antisepsis. Furthermore, HFs significantly contribute to drug delivery of topically applied substances. Nanoparticulate systems are excellently suited for follicular drug delivery as they entail the opportunity of directed drug trans...
Article
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Liposomal formulations are used to improve the safety and cellular absorption of conventional drugs by limiting their interaction with phagocytes. The uptake behaviour of these nanocarriers is affected by the blood composition, accordingly the presence of an anticoagulant in the blood could have a critical impact on the efficiency of the nanomedici...
Article
Colloidal nanoparticles (NPs) have attracted significant attention due to their unique physicochemical properties suitable for diagnosing and treating different human diseases. Nevertheless, the successful implementation of NPs in medicine demands a proper understanding of their interactions with the different proteins found in biological fluids. O...
Article
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Understanding the behavior of nanoparticles upon contact with a physiological environment is of urgent need in order to improve their properties for a successful therapeutic application. Most commonly, the interaction of nanoparticles with plasma proteins are studied under in vitro conditions. However, this has been shown to not reflect the complex...
Article
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The Right Combination: An artificial enzyme combination is presented that continuously produces NAD⁺ from oxygen and water and a tiny amount of pyruvate, without additional substrates and generating byproducts. Three enzymes are covalently encapsulated into a substrate‐permeable silica nanoreactor by a mild fluoride‐catalyzed sol–gel process. NAD⁺...
Article
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Nicotinamide adenine dinucleotide (NAD) is an essential coenzyme for numerous biocatalytic pathways. While in nature, NAD + is continuously regenerated from NADH by enzymes, all synthetic NAD + regeneration strategies require a continuous supply of reagents and generate byproducts, making these strategies unattractive. In contrast, we present an ar...
Article
Synthesis and crystallization of polyethylene with precisely positioned thiophene groups were used to produce polymer crystals with a conductive surface.
Article
Large scale solar energy storage in form of E-Z photoisomerisable organic materials requires avoiding of application of rare earths metals at any stage of the process used. Here we report...
Article
The development of enzyme modules by coupling several enzymes in confinement is of paramount importance to artificial biological reaction systems for efficient enzymatic reactions. Silica nanocapsules are ideal candidates for loading enzymes. Aqueous core silica nanocapsules have relatively been rarely reported due to the crux of difficulty in form...
Article
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The versatile design of stimuli‐responsive microparticles embedding valuable biomolecules has great potential in a variety of engineering fields, such as sensors, actuators, drug delivery, and catalysis. Here, results are reported on thermoresponsive core–gap–shell (TCGS) microcapsules made of poly(N‐isopropylacrylamide) (PNIPAm), which encapsulate...
Article
Hydrated inorganic salts are phase change materials (PCMs) with promising thermal energy storage capacity. However, their application is commonly restricted because of problems of phase segregation, incongruent melting, cycling instability, or supercooling. This work presents an encapsulation method of hydrophilic PCMs that allows for the incorpora...
Article
The function of an organic solar cell relies on making a contact surface between a donor and acceptor material. For efficient conversion of solar energy, this heterojunction must be maximized. Nanoparticulate systems already have a large surface-to-volume ratio per se. We increase the area of the heterojunction even further. Based on the miniemulsi...
Article
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Two-photon polymerization stereolithographic three-dimensional (3D) printing is used for manufacturing a variety of structures ranging from microdevices to refractive optics. Incorporation of nanoparticles in 3D printing offers huge potential to create even more functional nanocomposite structures. However, this is difficult to achieve since the ag...
Article
Heterogene Katalyse In ihrer Zuschrift auf S. 18526 beschreiben K. A. I. Zhang et al. die Synthese größenkontrollierter Nanopartikel aus kovalenten Triazingerüsten in Siliciumdioxid‐Kapseln.
Article
Heterogeneous Catalysis In their Communication on page 18368, K. A. I. Zhang et al. describe the synthesis of size‐controllable covalent triazine framework nanoparticles through a confined polymerization strategy within silica capsules.
Article
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Interleukin-2 (IL-2) is a T cell growth factor particularly required in regulatory T cell maintenance and memory T cell responses. High-dose IL-2 treatment was the first FDA-approved immunotherapy for cancer, while low-dose IL-2 administration has shown promise in allograft rejection and autoimmune and inflammatory diseases. However, its pleiotropi...
Article
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Opsonization of nanocarriers is one of the most important biological barriers for controlled drug delivery. The typical way to prevent such unspecific protein adsorption and thus fast clearance by the immune system is the covalent modification of drug delivery vehicles with poly(ethylene glycol) (PEG), so-called PEGylation. Recently, polyphosphoest...
Article
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Herein, we report the synthesis of carbohydrate and glycodendron structures for dendritic cell targeting, which were subsequently bound to hydroxyethyl starch (HES) nanocapsules prepared by the inverse miniemulsion technique. The uptake of the carbohydrate-functionalized HES nanocapsules into immature human dendritic cells (hDCs) revealed a strong...
Article
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T-cells are a type of lymphocyte (a subtype of white blood cells) that play a central role in cell-mediated immunity. Currently, adoptive T-cell immunotherapy is being developed to destroy cancer cells. In this therapy, T-cells are harvested from a patient's blood. After several weeks of growth in culture, tumor-specific T-cells can be reinfused in...
Article
Aim: For vaccines the combination between an antigen and adjuvants are both crucially important to trigger an effective immune response in dendritic cells. Innovative adjuvants like resiquimod or muramyldipeptide have their target protein inside the cell. Materials & methods: Up/downregulation and proteome expression was investigated for the adjuva...
Article
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Strategies for corrosion protection are required to prolong the life-span of metallic structures used by the construction, aerospace, and transport industries. Currently, there are no coatings that can provide at the same time information about the corrosion status of the coated metal and protect the metal against corrosive species and mechanical d...
Article
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We study the effect of entanglements on the glass transition of high molecular weight polymers, by the comparison of single-chain nanoparticles (SCNPs) and equilibrated melts of high-molecular weight polystyrene of identical molecular weight. SCNPs were prepared by electrospraying technique and characterized using scanning electron microscopy and a...
Article
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Synthetic assembly within living cells represents an innovative way to explore purely chemical tools that can direct and control cellular behaviour. We use a simple and modular platform that is broadly accessible and yet incorporates highly intricate molecular recognition, immolative and rearrangement chemistry. Short bi-modular peptide sequences u...
Article
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Introduction: Adoptive T-cell immunotherapy emerged as a powerful and promising cancer therapy, as the problem regarding the immuno-reaction between different donors and recipients can be avoided. However, this approach is challenging. After long cultivation and expansion under laboratory media conditions, T-cells are losing their viability and fu...
Cover Page
The use of miniemulsions containing chemical precursors in the disperse phase is a versatile method to produce nanoparticles and nanostructures of different chemical nature, including not only polymers, but also a variety of inorganic materials. The focus is on materials in which nanostructures of metal oxides are synthesized in processes that invo...
Article
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Construction of defined nanostructures is a key for enhancing the efficiency of heterogeneous catalytic materials. However, for metal‐free, organic conjugated polymer‐based photocatalysts, due to their mostly amorphous nature or specific synthetic routes, synthesis of defined nanostructures is still highly challenging. Here, we report the formation...
Article
Full-text available
Size‐controllable covalent triazine framework nanoparticles were synthesized through a confined polymerization strategy within silica capsules. They show a manifold increase in photocatalytic activity compared to the bulk system. Abstract For metal‐free, organic conjugated polymer‐based photocatalysts, synthesis of defined nanostructures is still...
Article
Controlling the degradation rates of polymers is crucial for their application in tissue engineering or to achieve degradation of the polymers in the wastewater purification. As hydrophobic polyesters often exhibit very slow degradation rates, we report here increased biodegradation rates of poly(globalide-co-ε-caprolactone) copolymers (PGlCL) prod...
Article
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The anticancer efficacy of photodynamic therapy (PDT) is limited due to the hypoxic feature of the solid tumors. Herein, we report synergistic PDT/chemotherapy with integrated tandem Fenton reactions mediated by ovalbumin encapsulation for improved in vivo anticancer therapy via an enhanced reactive oxygen species (ROS) generation mechanism. The bi...
Article
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Synergistic photodynamic/chemotherapy is reported with integrated tandem Fenton reactions enabled by ovalbumin co‐encapsulation. In vivo anticancer therapy is improved via an enhanced reactive oxygen species (ROS) generation mechanism. Abstract The anticancer efficacy of photodynamic therapy (PDT) is limited due to the hypoxic features of solid tu...
Article
A protein coat, termed the protein corona, assembles around the nanocarriers´ surface once it gets in contact with a biological environment. We show that the media used for the washing of protein corona can be crucial. This is true for the downstream analysis as well as for the pre-coating used in in vitro or in vivo. This has been widely overlooke...
Article
Amphiphilic polyphenylene dendrimers (PPDs) with distinct lipophilic and postively or negatively charged surface groups were adsorbed onto liposomes and their impact on protein adsorption in the blood plasma was studied....
Article
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Lignin-based nano- and microcarriers are a promising biodegradable drug delivery platform inside of plants. Many wood-decaying fungi are capable of degrading the wood component lignin by segregated lignases. These fungi are responsible for severe financial damage in agriculture and many of these plant diseases cannot be treated today. However, enzy...
Article
Full-text available
Bio-orthogonal reactions have become an essential tool to prepare biomaterials, for example, in the synthesis of nanocarriers bio-orthogonal chemistry allows circumventing common obstacles related to the encapsulation of delicate payload or the occurrence of uncontrolled side reactions, which significantly limit the range of potential payloads to e...