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Katerina Grigoriadou

Katerina Grigoriadou
Institue of Plant Breeding and Genitic Resourses, HAO-DEMETER, Greece · Tissue Culture Laboratory / Laboratory of Conservation and Evaluation of Native Plants

About

52
Publications
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Introduction
Dr. Katerina Grigoriadou is specialized in plant propagation (in vitro and conventional methods). She works on projects related new product development and cost reduction in cultivation of MAPs and fruits. Hers current research interest focuses on in vitro production of MAPs secondary metabolites of greek native species.

Publications

Publications (52)
Article
Full-text available
Alkanna tinctoria Tausch. (Boraginaceae), commonly known as alkanet or dyers’ bugloss/alkanet, is a perennial plant rich in naphthoquinone enantiomers, such as Alkannin and Shikonin (A/S), which possess a wide range of pharmaceutical properties and are used as cosmetics, food additives, and natural dyes. This plant is mostly exploited from the wild...
Article
Full-text available
The processing of medicinal and aromatic plants (MAPs) results in the production of a significant amount of plant by-products; herbal material of inferior quality and/or unusable plant parts that are not commercially exploitable. An extensive study of Greek native species was performed toward the production of innovative bioactive products using as...
Article
Full-text available
Kefalograviera is a well-known hard Greek cheese. The aim of this study was to determine how milk produced from ewes fed omega-3-enriched diets could influence the microbiota as well as the chemical composition of Kefalograviera cheese. At the start of the trial, 30 dairy ewes (Lesvos and Chios crossbreed) were selected and fed a conventional diet,...
Article
Full-text available
The traditionally edible aerial parts of rock samphire (Crithmum maritimum L.) could be a valuable functional food or feed ingredient due to their high antioxidant capacity, ascorbic acid content, and rich content in secondary metabolites such as phenolics and flavonoids. The first objective of this study was to evaluate eighteen genotypes derived...
Article
Full-text available
Introduction Bioactive constituents of medicinal-aromatic plants used as feed additives may affect the metabolic profile and oxidative stability of hen eggs. Objectives To determine the effects of dietary supplementation with a mixture of dried oregano, thyme, sideritis tea and chamomile on laying hen performance, egg quality parameters, and oxida...
Article
Full-text available
Two Gram-negative, aerobic, rod-shaped and yellow-orange pigmented bacterial strains (LMG 31523T and LMG 31524) were isolated from roots of wild-growing Alkanna tinctoria plants collected near Thessaloniki, Greece. Analysis of their 16S rRNA gene sequences revealed that they form a separate cluster related to the genus Roseomonas. A comparative who...
Article
Full-text available
The continual search for new attractive ornamentals and innovative natural medicinal products is the main focus of floricultural and pharmaceutical industries worldwide. Aiming to introduce two new Cretan endemic species in the commercial trade, Acantholimon androsaceum (Jaub. & Spach) Boiss. and Limonium chersonesum Erben & Brullo (Plumbaginaceae)...
Article
Full-text available
Human needs and concomitant commercial trade to date trigger the demand of new ornamental plants and new natural medicinal products. The current study includes preliminary seed germination trials and presents the development of effective vegetative propagation protocols for Lomelosia minoana subsp. minoana (Dipsacaceae) and Scutellaria hirta (Lamia...
Article
Full-text available
Alkannin and shikonin (A/S) are enantiomeric naphthoquinones produced in the roots of certain plants from the Boraginaceae family such as Lithospermum spp. and Alkanna spp. They possess antimicrobial, anti-tumoral and wound healing properties. The production of secondary metabolites by Alkanna tinctoria might be influenced by its endomicrobiome. To...
Conference Paper
Recently, novel dietary strategies are under examination, aiming to improve the sensory characteristic, as well as the potential health benefits of animal products, such as cheeses produced by sheep milk. This study examined the possible changes in the chemical composition and fatty acid profile of Greek kefalograviera cheese, caused by the partial...
Preprint
Full-text available
Conservation and sustainable exploitation of threatened endemic plants with medicinal and/or horticultural/ornamental value can be achieved through the development of effective propagation protocols. After unveiling the bioclimatic preferences of Carlina diae (Asteraceae) with geographic information systems (GIS), four propagation trials were condu...
Conference Paper
Soybean and its by-product soybean meal are arguably the most commonly proteinateous feed ingredients used in farm animals. Nevertheless, these feed ingredients are produced in Europe in very low quantities; therefore, large amounts are imported annually, which necessitate high economical cost and environmental impact. It is possible that novel fee...
Article
Full-text available
EIP-AGRI Focus Group Plant-based medicinal and cosmetic products https://ec.europa.eu/eip/agriculture/en/focus-groups/diversification-opportunities-through-plant-based
Technical Report
Full-text available
EIP-AGRI Focus Group Plant-based medicinal and cosmetic products https://ec.europa.eu/eip/agriculture/en/focus-groups/diversification-opportunities-through-plant-based
Article
Full-text available
During 2019, the EIP-AGRI Focus Group on Plant-based Medicinal and Cosmetic Products has analysed the key factors that influence farmers and foresters to enter the Medicinal and Aromatic Plant (MAP) sector. The main question was: ‘How to create diversification opportunities for farmers through innovative value chains of plant-based medicinal and co...
Article
Full-text available
Salvia sclarea L. is valuable for its essential oils and other perfumery products used in the food and pharmaceutical industries. The aim of this study was to develop a rapid micropropagation protocol and cultivate S. sclarea in northern Greece for essential oil and sclareol production. The initial plant material used was S. sclarea seeds (biotype...
Article
Full-text available
Worldwide, many medicinal and aromatic plants (MAPs) are still collected from the wild and only a small fraction of them are exclusively sourced from cultivation. This practice when performed non-sustainably threatens species and populations. Micropropagation of MAPs is a powerful tool to conserve rare, threatened, and valuable MAPs, and to massive...
Article
Thymus sibthorpii Benth. (Lamiaceae), with accession number 01,1796-22, is a biotype of native Greek thyme with ascending stems and potential use as a new medicinal-aromatic crop and ornamental plant. An efficient and reliable protocol for in vitro clonal propagation of T. sibthorpii from nodes and meristem tip explants was developed. Shoot prolife...
Article
Full-text available
Maintaining the genotype through asexual propagation is necessary for species with conservation priority (rare and threatened). Silene fabaria subsp. domokina (Caryophyllaceae) is a range-restricted Greek endemic of ornamental value. In autumn, softwood top cuttings (2-3 cm) were cut from mother plants kept inside the greenhouse and their base was...
Conference Paper
Μελετήθηκε η ποιότητα και συντηρησιμότητα μήλων ‘Πιλαφά Ντελίσιους’ από 4 εμπορικούς μηλεώνες με διαφορετικά χαρακτηριστικά της περιοχής Τεγέας Τριπόλεως: μηλεώνα βιολογικό με ώριμα δέντρα (Δ), μηλεώνα ώριμο συμβατικό (Σ) (και τα δύο διαμορφωμένα σε κύπελλο και υποκείμενο σπορόφυτο), μηλεώνα εντατικό 8 ετών σε υποκείμενο ΜΜ106 (Κ) και μηλεώνα εντατ...
Article
Full-text available
In order to develop conservation protocols for Campanula incurva, the geographical information systems (GIS) were used to unveil its ecological requirements; this facilitated the selection of substrates and of appropriate temperatures for cultivation and guided propagation experiments and acclimatization. Seed germination was tested under (i) dark,...
Article
In vitro mass propagation was achieved for four diverse genotypes of the wild carnation Dianthus petraeus, originating from four different geographical areas in Greece. Adventitious shoot proliferation was induced on Murashige and Skoog (MS) medium supplemented with 0.5, 1, and 2 mg l-1 of TDZ in combination with 0, 0.1 and 0.5 mg l-1 of NAA, using...
Article
Full-text available
A Geographical Information Systems (GIS)-facilitated approach for the in vitro propagation and ex situ conservation of the conservation priority species Achillea occulta is presented. To realize the species’ ecological requirements, the coordinates of the original habitat were linked with thematic layers derived from digital databases in a GIS envi...
Article
Full-text available
The effects of six basal media on in vitro shoot proliferation of the greek grapevines Vitis vinifera L. cv. ‘Malagouzia’ and ‘Xinomavro’ were investigated. Galzy and Zlenco proved to be the most effective for ‘Malagouzia’ and ‘Xinomavro’, respectively. If only BA was present in the medium, shoot development was poor and the plantlets were chloroti...
Article
Full-text available
The Balkan Botanic Garden of Kroussia (BBGK) is dedicated to the ex situ conservation of native plants of Greece and the Balkans. The BBGK has formulated a conservation strategy for the collection of wild plant material for propagation, prioritizing mainly the endemic, rare, endangered, threatened and vulnerable plants of Europe found in different...
Article
Full-text available
SUMMARY Grapevine leafroll-associated virus 1 (GLRaV-1) and Grapevine rupestris stem pitting-associated virus (GRSPaV) were eradicated from Vitis vinifera L. cv. Agiorgitiko by combining in vitro thermotherapy and tissue culture. GRSPaV is known to be quite recalcitrant to elimination, whereas GLRaV-1 is more easily knocked out. In this study, the...
Article
Full-text available
Crithmum maritimum (Apiaceae), a perennial halophyte native in Greece, could be used as an alternative culture at problematic soils. It presents significant economical potentials as its essential oils are in high demand from the medicinal and cosmetic industry. The response of the species on invitro conditions was studied. MS proved to be the most...
Article
Full-text available
Plant material originating in wild populations, collected from various areas of Greece, has been maintained at the Laboratory of Conservation and Evaluation of Native and Floricultural Species. In the frame of the conservation program of the Balkan Botanic Garden of Kroussia (BBGK) the original collections count more than 1700 accession numbers of...
Chapter
Full-text available
This study outlines a pioneer initiative concerning the native plant conservation that is considered as one of the most important plant refuges in Europe. The Balkan Botanic Garden of Kroussia has designed a system built on people, infrastructure and a strategy of seven hierarchical and complementary policies. To formulate and implement this strate...
Article
Full-text available
Micropropagation of olive has not yet been very successful. Shoot proliferation rate is generally low and cultivar dependent; the formation of adventitious roots in many micropropagated olive cultivars is difficult and the percentage of post-transplanting losses high. The use of liquid medium in a novel temporary immersion system has increased prol...
Article
Full-text available
Among the activities of the laboratory of Floriculture of the National Agriculture Research Foundation (NAGREF) are the collection, conservation and study of native Mediterranean and Balkan species. These are materialized through the newly established Balkan Botanic Garden of Kroussia (BBGK), situated in the area of central Macedonia, Greece. In th...
Chapter
Full-text available
A novel temporary immersion system (TIS), designed at the laboratory of VITRO HELLAS S.A., was used for the liquid culture of olive microshoots during their proliferation phase. The results were compared with those obtained in agar solidified medium, which was used as control, and with other bioreactor systems including the LifeReactor , liquid cul...
Article
Full-text available
The Balkan Botanic Garden situated at Kroussia mountains and the Balkan Horticultural Research Institute in Thermi/Thessaloniki aspire to become a major scientific and educational center for the Mediterranean and Balkan Flora. Until now, the Garden's physical infrastructure has been established, the national living plant collection has been initiat...
Article
Full-text available
Microshoots of the Greek olive cv. Chondrolia Chalkidikis were cultured in vitro in WPM supplemented with 4 μM zeatin. The effect of various auxin concentrations (IBA, NAA) on root induction was studied. Rooting percentage up to 70% was achieved by the combination of 12 μM IBA + 3 μM NAA although abscission of shoot tips and leaves was common. The...
Article
Full-text available
Microcuttings of strawberry tree (Arbutus unedo L.) were produced in WPM supplemented with MS vitamins, sucrose 20 g l-1, agar 5 g l -1 and 22 μM BA. Experimentation related to in vitro rooting was carried out in glass jars containing 70 ml of WPM supplemented with either IBA (0, 2.5, 5, 10 μM) or IAA (0, 10, 20 μM). Highest percentages of rooting...
Conference Paper
Full-text available
Potato minitubers and Lagerstroemia indica (grape myrtle) microshoots produced in vitro by a bioreactor system by Osmotek Ltd. (Israel) were compared by qualitative and qualitative means to respective ones produced conventionally in glass culture jars. 'Spunta' potato single node segments were cultured for 12 weeks, in complete darkness, in both li...
Article
Full-text available
Actively growing shoots of potted greenhouse-grown strawberry tree (Arbutus unedo L.) were initially sterilised and established in basal woody plant medium containing 11.1 μM BA. Optimum shoot proliferation was achieved on a basal WPM containing MS vitamins, sucrose, agar and 22.2 μM BA. Microshoots rooted successfully in basal in vitro medium cont...
Article
The effect of basal media (Woody Plant Medium (WPM), Quoirin and Lepoivre (QL) and Olive Medium (OM)) and of various concentrations of cytokinins (6-benzyladenine (BA), zeatin, 6-(,-dimethylallyl-amino) purine (2iP)), solely or in combinations with each other and with gibberellic acid (GA3), on in vitro shoot proliferation of the greek olive cultiv...
Article
Full-text available
In vitro production of multiple shoots, in vitro and ex vitro rooting and acclimatization were examined. Approximately 3 shootlets/explant were achieved after 4 weeks of culture in MS basic salts supplemented with 3μM BAP, 0.3μM GA3, 0.05μM NAA, 20% sucrose (w/v) and 0.5% (w/v) agar. The addition of 5.4μM of NAA and 5.0 μM IBA in MS basic medium re...
Article
The effect of culture media (Murashige & Skoog, MS; 1/2 MS; woody plant medium, WPM; and Quoirin & Lepoivre, QL salts with MS vitamins), pH (5.2-7), Ca and benzyladenine (BA, 0-10 μM) concentration, and combination of BA with gibberellic acid (GA3) on shoot proliferation and shoot tip necrosis was studied in shoot cultures of pear cvs 'William's' a...
Article
Full-text available
Large scale, commercial production of potato minitubers, cvs Spunta, Jaerla and Kennebec, has been carried out in VITRO HELLAS S.A. since 1995, according to protocols based on in vitro methods. Apical meristems were cultured on MS medium supplemented with 1 μM IBA, 20 g/l sucrose and 6 g/l agar (pH 5.8). Subculture was carried out every 15 days. Mu...

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Projects

Project (1)
Project
-Technological development within three Hellenic Agricultural Organization ELGO-Demeter Institutes (Institute of Plant Breeding and Genetic Resources, Animal Research Institute and Soil and Water Resources Institute) on next generation omics infrastructure -Exploitation of animal and plant genetic resources -New infrastructures