Katarzyna Szot-Karpińska

• PhD
• Research Associate at Polish Academy of Sciences

The sponge-like hydrogenated titania nanotubes (S-TiO2-NTs) modified with M13 bacteriophages were investigated for bacterial detection. The electrodes used in this study were fabricated via anodisation and calcination in a hydrogen atmosphere. Electrochemical methods were used to study the capacitive and Faradaic currents of the S-TiO2-NTs electrod...

M13 bacteriophages (in short, phages) have an intrinsic ability to infect Escherichia coli bacteria but can be engineered to exhibit specific peptides on their surface which enables them to selectively bind to diverse targets. Therefore, such phages can be used as a sensing element in a biosensor. Consequently, our aim is to assess the effectivenes...

Point-of-care testing (POCT) devices play a crucial role as tools for disease diagnostics, and the integration of biorecognition elements with electronic components into these devices widens their functionalities and facilitates the development of complex quantitative assays. Unfortunately, biosensors that exploit large conventional IgG antibodies...

Point-of-care testing (POCT) devices play a crucial role as tools for disease diagnostics. The integration of biorecognition elements with electronic components into these devices widens their functionalities and facilitates the development of complex quantitative assays. Unfortunately, biosensors that exploit large conventional IgG antibodies to c...

We investigate the interactions between C-reactive protein (CRP) and new CRP-binding peptide materials using experimental (biological and physicochemical) methods with the support of theoretical simulations (computational modeling analysis). Three specific CRP-binding peptides (P2, P3, and P9) derived from an M13 bacteriophage have been identified...

Phage-derived affinity peptides have become widespread thanks to their easy selection via phage display. Interactions between a target protein and its specific peptide are similar to those between antibodies and antigens. The strength of these non-covalent complexes may be described by the dissociation constant (Kd). In this paper, protein-specific...

Phage-derived affinity peptides have become widespread thanks to their easy selection via phage display. Interactions between a target protein and its specific peptide are similar to those between antibodies and antigens. The strength of these non-covalent complexes may be described by the dissociation constant (Kd). In this paper, protein-specific...

C-reactive protein (CRP) is an inflammation biomarker that should be quantified accurately during infections and healing processes. Nanobodies are good candidates to replace conventional antibodies in immunodiagnostics due to their inexpensive production, simple engineering, and the possibility to obtain higher binder density on capture surfaces. S...

Recently, bacteriophage particles have started to be applied as a new biomaterial for developing sensing platforms. They can be used as both a recognition element or/and as building blocks, template/scaffold. In this paper, we studied a bacteriophage selected through phage-display technology. The chosen bacteriophage acted as a building block for c...

Bacteriophages are interesting entities on the border of biology and chemistry. In nature, they are bacteria parasites, while, after genetic manipulation, they gain new properties, e.g., selectively binding proteins. Owing to this, they may be applied as recognition elements in biosensors. Combining bacteriophages with different transducers can the...

In this work we discuss a new label-free biosensing device based on indium tin oxide (ITO) overlaid section of a multimode optical fiber fused silica core. The sensor has been used to optical measurements also simultaneously interrogated electrochemically (EC). Due to optimized thickness and optical properties of ITO film, a lossy-mode resonance (L...

Bacteriophage material (M13, wild-type) deposited as a film onto a poly-ethylene-terephthalate (PET) substrate (6 microm thick with a 20 microm diameter laser-drilled microhole) has been investigated for ion conductivity and ionic current rectification effects for potential applications in membranes. The M13 aggregate membrane forms under acidic co...

In this work we discussed a label-free biosensing application of long-period gratings (LPGs) optimized in refractive index (RI) sensitivity by deposition of thin tantalum oxide (TaO x ) overlays. Comparing to other thin film and materials already applied for maximizing the RI sensitivity, TaO x offers good chemical and mechanical stability during i...

The constant search for new materials applicable in electrochemical sensors and electronic devices is encouraging scientists to turn to interdisciplinary research. Particularly interesting in this context is the combination of biology with chemistry and material sciences. Recently, bacteriophages (viruses that infect bacteria) have been increasingl...

Indium-tin-oxide-coated long-period fiber grating was used simultaneously as a working electrode and an optical probe sensitive to refractive index changes in combined opto-electrochemical setup. The optical response of the sensor was strongly dependent on the applied potential.

Carbon nanomaterials have aroused substantial interest in various research fields. [...]

With the advent of nanotechnology, carbon nanomaterials such as carbon nanofibers (CNF) have aroused substantial interest in various research fields including energy storage and sensing. Further improvement of their properties might be achieved via application of viral particles such as bacteriophages. In this report we present a filamentous M13 ba...

Over the last several years bacteriophages have appeared as an interesting addition to electrochemical devices, both as recognition elements and as scaffolds and templates for novel electrode materials. Their natural ability for selective binding to specific analytes and the versatility offered by genetic modification and selection through phage di...

Most recent application of carbon nanoparticles in electroanalysis are rewieved. After brief introduction on a variety of carbon nanomaterials, the methods of functionalisation or doping of carbon nanoparticles are presented. Next the techniques of their immobilization on the electrode surface are reviewed with special emphasis on layer-by-layer de...

The electrocatalytic oxidation of neurotransmitters on the electrodes modified with oppositely charged carbon nanoparticles has been investigated. These nanoparticles were deposited at the electrode from the aqueous suspensions via a layer-by-layer method. The electrocatalytic response was evaluated by cyclic voltammetry, differential pulse voltamm...

Herein, we report a colorimetric immunosensor for T7 bacteriophage based on gold nanoparticles modified with covalently bonded anti-T7 antibodies. The new immunosensor allows for a fast, simple and selective detection of T7 virus. T7 virions form immunological complexes with the antibody modified gold nanoparticles which causes them to aggregate. T...

We report on the development of a nanocarbon based anode for sensing of ascorbic acid (AA). The oxidation of AA on this anode occurs at a quite low overpotential which enables the anode to be connected to a biocathode to form an ascorbic acid/O2 biofuel cell that functions as a self-powered biosensor. In conjunction with a Prussian blue electrochro...

Hydrogenases are the key enzymes for hydrogen metabolism in many microorganisms. Due to the high efficiency and specificity they developed for H2 oxidation, they have been recently used as biocatalysts for an efficient H2/O2 biofuel cell. In this work we explore new properties of an O2-, CO- and T degrees-resistant hydrogenase. Enzyme immobilizatio...

Carbon nanoparticulate film electrodes were prepared by alternative immersion of indium tin oxide plates into suspension of positively and negatively charged carbon nanoparticles. Their average thickness is in the range of 30–300 nm as determined by atomic force microscopy and depends on the number of immersion and withdrawal steps. After adsorptio...

The effect of linker of three-dimensional, hydrophilic-carbon-nanoparticle film-electrodes prepared by layer-by-layer method on redox probe accumulation and bioelectrocatalytic dioxygen reduction was studied and compared for two different electrode scaffolds. The linker in both of these scaffolds was based on the same ionic liquid sol–gel precursor...

Single-step electrodeposition of nanocomposite silicate materials based on hydrophilic carbon nanoparticles (CNPs) and a hydrophobic sol-gel precursor with the help of an electrode|organic-phase|aqueous-electrolyte three-phase junction is presented. A tin-doped indium oxide electrode is immersed into a cell filled with two immiscible liquids. The u...

Carbon ceramic electrodes were prepared by sol–gel processing of a hydrophobic precursor – methyltrimethoxysilane (MTMOS) – together with dispersed graphite microparticles according to a literature procedure. Bilirubin oxidase (BOx) was adsorbed on this electrode from buffer solution and this process was followed by atomic force microscopy (AFM). T...

Electrodes modified with sol-gel encapsulated laccase (isolated from Cerrenaunicolor) exhibiting mediated or mediatorless bioelectrocatalytic dioxygen reduction activity were inspected using confocal laser scanning microscopy, atomic force microscopy and scanning electrochemical microscopy. Potential-driven leaching of the redox mediator 2,2'-azino...

Thin silicate films with immobilised enzymes catalysing dioxygen reduction, i.e. laccase and bilirubin oxidase (BOD), were deposited on glass and poly(methyl 2-methylpropenoate) (Plexiglas) surfaces in a sol-gel process by sol drop evaporation. Scanning electrochemical microscopy (SECM) images and approach curves were recorded using hexacyanoferrat...

Three dimensional carbon film electrodes were prepared from oppositely charged carbon nanoparticles (ca. 9 to 18nm diameter) by a layer-by-layer approach. This was done by alternative immersion of indium tin oxide plates into suspension of positively and negatively charged particles. A stable film is formed already after single immersion and withdr...

Raman spectroscopy studies of oxidation of 3,5-dimethoxy-4- hydroxybenzaldehydrazine (syringaldazine) encapsulated in silica and composite carbon electrodes were performed. The presence of reduced and oxidized forms of syringaldazine present at various potentials was determined using Raman spectroscopy. The structure of the oxidized form was discus...

Laccase from Cerrena unicolor was adsorbed on hydrophilic carbon nanoparticles (diameter = ca. 7.8 nm) modified with phenyl sulfonate groups and immobilized on an ITO electrode surface in a sol–gel processed silicate film. As shown by scanning electron and atomic force microscopies, the nanoparticles are evenly distributed on the electrode surface...

Using one step procedure we have prepared electrodes using single-walled carbon nanotubes non-covalently functionalised with 1,3,6,8-pyrenetetrasulfonate or 1-pyrenesulfonate immobilised together with the enzyme laccase (from Cerrena unicolor) in a silicate sol–gel matrix. Both electrodes exhibit mediatorless electrocatalytic activity towards dioxy...

Multiwalled carbon nanotubes were entrapped in sol-gel processed hydrophilic silicate thin film on tin-doped indium oxide support. Microscopic images show that the nanotubes form large agglomerates of largely separated nanotubes covered by silicate film. The measurements of capacitive current prove that approximately 10% of them remain electrochemi...

Multilayer hydrophilic carbon nanoparticles modified electrodes are prepared by a layer-by-layer method combined with sol–gel processing. A tin doped indium oxide substrate is alternatively immersed in sulfonated carbon nanoparticles solution and a sol containing the ionic liquid precursor 1-methyl-3-(3-trimethoxysilylpropyl)imidazolium bis(trifluo...

A hydrophilic carbon nanoparticle–sol-gel electrode with good electrical conductivity within the sol-gel matrix is prepared. Sulfonated carbon nanoparticles with high hydrophilicity and of 10–20nm diameter (Emperor 2000) are co-deposited onto tin-doped indium oxide substrates employing a sol-gel technique. The resulting carbon nanoparticle-sol-gel...

The tin-doped indium oxide (ITO) electrode served as a suitable support for the mediator 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) and extracellular laccase from Cerrena unicolor encapsulated in sol–gel processed thin hydrophilic silicate/polyallylamine film. For some experiments the electrode was premodified with tin-doped indium oxide na...

Tin-doped indium oxide (ITO) nanoparticles (ca. 20 nm in diameter) were deposited onto electrode surfaces employing a simple immersion, withdrawal and solvent evaporation method. The resulting ITO films are irregularly packed and highly porous and they promote electron transport. When water insoluble redox liquids are immobilized within the porous...