Katarzyna Retzer

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Verified

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• Dr.nat.techn.
• PI at BOKU University

Plant growth and productivity are orchestrated by a network of signaling cascades involved in balancing responses to perceived environmental changes with resource availability. Vascular plants are divided into the shoot, an aboveground organ where sugar is synthesized, and the underground located root. Continuous growth requires the generation of e...

Arabidopsis PIN2 protein directs transport of the phytohormone auxin from the root tip into the root elongation zone. Variation in hormone transport, which depends on a delicate interplay between PIN2 sorting to and from polar plasma membrane domains, determines root growth. By employing a constitutively degraded version of PIN2, we identify brassi...

SnRK1 (Sucrose non-fermenting-1-Related Kinase 1) is a master regulator of cellular energy homeostasis in plants, coordinating developmental and metabolic responses under environmental and internal stress conditions. Here, we demonstrate that its catalytic subunit, KIN10, orchestrates organ-specific growth-defense programs in Arabidopsis thaliana t...

Root growth directionality is critical for plant survival, optimizing anchorage and resource acquisition. While the role of hormonal signaling in root gravitropism is well established, the contribution of metabolic status remains less understood. Here, we investigate the function of the catalytic SnRK1 subunit KIN10 in integrating carbon availabili...

Root phenotyping is a challenging task that would require monitoring root growth in soil under dark conditions to mimic natural conditions, while allowing the shoot to grow in light. Most existing methods involve exposing the roots to light, which substantially alters their growth and function. In this paper, we present an improved imaging system t...

Roots and microbiomes enhance nutrient uptake, drought resilience, and disease resistance, improving agricultural sustainability. The BarleyMicroBreed project studies 568 barley varieties to identify root traits that recruit beneficial microbes. Non-invasive imaging tools enable real-time observation of root growth and root-microbe interactions. Tr...

Plant development is under the morphogenic control of auxin. In addition to biosynthesis and metabolism, auxin concentration gradients are maintained by directional intercellular transport via PIN-FORMED (PIN) auxin efflux carriers. Although the structure-function properties of PINs have been described, it is still unclear whether individual member...

The need for increasing for crop productivity leads to a higher usage of synthetic fertilizers, which has tremendous effects on the environment. Nitrogen (N) is a crucial plant macronutrient, but the production of synthetic N fertilizer and its leakage into aquatic systems represent sources of environmental damage. To reduce the usage of synthetic...

The “Centre for Experimental Plant Biology”, a joint project of the Institute of Experimental Botany of the Czech Academy of Sciences and CEITEC (represented by Mendel and Masaryk Universities), focused on elucidating the mechanisms of plant responses to abiotic and biotic stresses and their combinations at the cellular level, in intact plants duri...

Roots are sensors evolved to simultaneously respond to manifold signals, which allow the plant to survive. Root growth responses, including the modulation of directional root growth, were shown to be differently regulated when the root is exposed to a combination of exogenous stimuli compared to an individual stress trigger. Several studies pointed...

Plants move to acquire energy/resources. Plants require energy/resources to move. As sessile organisms, plants rely on fine-tuning organ movement to ensure their survival and productivity. Even a subtle loss of directional growth orchestration can have a big impact on plant growth, when the plant is impaired to efficiently react to simultaneousl...

Directionality in the intercellular transport of the plant hormone auxin is determined by polar plasma membrane localization of PIN-FORMED (PIN) auxin transport proteins. However, apart from PIN phosphorylation at conserved motifs, no further determinants explicitly controlling polar PIN sorting decisions have been identified. Here we present Arabi...

Plants rely on fine-tuning organ movement to ensure their survival and productivity. Even subtle loss of directional growth orchestration can result in a huge impact when the plant is impaired to adapt to everchanging environment, where it is exposed to manifold exogenous stimuli simultaneously. We present a newly designed chamber to obtain live im...

Intracellular sorting and the abundance of sessile plant plasma membrane proteins are imperative for sensing and responding to environmental inputs. A key determinant for inducing adjustments in protein localization and hence functionality is their reversible covalent modification by the small protein modifier ubiquitin, which is for example respon...

Mechanical responses of individual cells to plant internal and external stimuli modulate organ movement and ensure plant survival as sessile organism in a constantly changing environment. The root is a complex, three-dimensional object, which continuously modifies its growth path. Autonomous and paratonic root movements are both orchestrated by dif...

Phosphatidylinositol 4-kinases (PI4Ks) are the first enzymes that commit phosphatidylinositol into the phosphoinositide pathway. Here, we show that Arabidopsis thaliana seedlings deficient in PI4Kβ1 and β2 have several developmental defects including shorter roots and unfinished cytokinesis. The pi4kβ1β2 double mutant was insensitive to exogenous a...

As sessile organisms, plants rely on fine-tuning organ movement to ensure their survival and productivity. Even a subtle loss of directional growth orchestration can have a big impact on plant growth, when the plant is impaired to efficiently react to simultaneously occurring exogenous stimuli in an everchanging environment. To understand the impa...

Directional root growth control is crucial for plant fitness. The degree of root growth deviation depends on several factors, whereby exogenous growth conditions have a profound impact. The perception of mechanical impedance by wild-type roots results in the modulation of root growth traits, and it is known that gravitropic stimulus influences dist...

Directional root growth is orchestrated depending on manifold exogenous and intrinsic stimuli. I present how to design experimental setups to reduce unnecessary exogenous stimuli while studying the modulation of root growth control. And to which extent root movement is coordinated by shootward auxin flux mediated by AUX1 and PIN2.

The root is the below-ground organ of a plant, and it has evolved multiple signaling pathways that allow adaptation of architecture, growth rate, and direction to an ever-changing environment. Roots grow along the gravitropic vector towards beneficial areas in the soil to provide the plant with proper nutrients to ensure its survival and productivi...

Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators. Combining approaches of genetics, biochemistry and...

Standard lab conditions, which include seedling growth with exposed roots to direct illumination and sugar supplementation to the growth medium change root growth and root trait establishment.

Plant cell properties are defined by its proteome and metabolome, which depend on the genetic background together with environmental conditions. Mechanical responses of individual cells to plant internal and external stimuli modulate organ movement and ensure thereby plant survival as sessile organism in a constantly changing environment. The root...

Plant growth is continuously modulated by endogenous and exogenous stimuli. By no means the only, but well described, signaling molecules produced in plants and distributed through the plant body to orchestrate efficient growth are photosynthates. Light is a potent exogenous stimulus that determines, first, the rate of photosynthesis, but also the...

https://www.youtube.com/watch?v=C-D1BRR1mMM&t=9s https://www.youtube.com/watch?v=64xutAFco74&t=28s https://twitter.com/KatarzynaRetzer #drootsystem The sessile life style of plants forces them to adapt constantly to changing environmental conditions. Establishment of auxin gradients underpin modulation of plant architecture during development...

Together with auxin transport, auxin metabolism is a key determinant of auxin signalling output by plant cells. Enzymatic machinery involved in auxin metabolism is subject to regulation based on numerous inputs, including the concentration of auxin itself. Therefore, experiments characterizing altered auxin availability and subsequent changes in au...

Plant roots are very plastic and can adjust their tissue organization and cell appearance during abiotic stress responses. Previous studies showed that direct root illumination and sugar supplementation mask root growth phenotypes and traits. Sugar and light signaling where further connected to changes in auxin biosynthesis and distribution along t...

Auxin metabolism is, together with auxin transport, a key determinant of auxin signalling output in plant cells, yet details on the underlying mechanisms and factors involved are still largely unknown. Processes involved in the auxin metabolism are subject to regulation based on numerous signals, including auxin concentration itself. Altered auxin...

In over 40 years of research on the cellular uptake of auxin it is somewhat chastening that we have elaborated so little on the original kinetic descriptions of auxin uptake by plant cells made by Rubery and Sheldrake in 1974. Every aspect of that seminal work has been investigated in detail, and the uptake activity they measured is now known to be...

Strigolactones are a particular class of plant metabolites with diverse biological functions starting from the stimulation of parasitic seed germination to phytohormonal activity. The expansion of parasitic weeds in the fields of developing countries is threatening the food supply and calls for simple procedures to combat these weeds. Strigolactone...

The canonical auxin receptor complex mediates gene expression, but it is also necessary for responses far too rapid to be mediated by transcription. An innovative setup that uses advanced microscopy and microfluidics can record auxin-induced changes within 30 seconds during root growth.

Coordination of plant development requires modulation of growth responses that are under control of the phytohormone auxin. PIN-FORMED plasma membrane proteins, involved in intercellular transport of the growth regulator, are key to the transmission of such auxin signals and subject to multilevel surveillance mechanisms, including reversible post-t...

Auxins, a group of plant signalling compounds, ensure proper growth and development of the plant in relation to both external and internal stimuli. Within a plant, auxin is distributed asymmetrically, thus creating local auxin maxima and minima. Such asymmetric auxin distribution underlies many developmental and stress adaptation processes and faci...

Plants are sessile organisms and need to react to environmental changes rapidly to ensure proper development. Tightly controlled protein turnover guarantees that proteins are build up or removed at the right time, at the required place and in appropriate concentrations. This also applies to directed auxin transport, which regulates multitude of...

Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules present in all eukaryotes. In plants, MAPK cascades were shown to regulate cell division, developmental processes, stress responses, and hormone pathways. The subgroup A of Arabidopsis MAPKs consists of AtMPK3, AtMPK6, and AtMPK10. AtMPK3 and AtMPK6 are activ...

Recently established links between the tomato cyclophilin A-type protein DIAGEOTROPICA and the regulation of polar auxin transport provide first mechanistic insights into the function of this enigmatic locus. Copyright © 2015 Elsevier Ltd. All rights reserved.

Polar transport of the phytohormone auxin throughout plants shapes morphogenesis and is subject to stringent and specific control. Here, we identify basic cellular activities connected to translational control of gene expression as sufficient to specify auxin-mediated development. Mutants in subunits of Arabidopsis Elongator, a protein complex modu...

Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules present in all eukaryotes. In plants, MAPK cascades were shown to regulate cell division, developmental processes, stress responses and hormone pathways. The subgroup A of Arabidopsis MAPKs consists of AtMPK3, AtMPK6, and AtMPK10. AtMPK3 and AtMPK6 are activa...

From the very beginnings, attempts to identify mechanisms underlying polar auxin transport in higher plants have been intimately linked to studies on the regulation of plant tropisms. Already in the nineteenth century Charles Darwin came up with a concept, suggesting that a transmissible signal might be involved in controlling directional plant gro...

Key message: We show that DCN1 binds ubiquitin and RUB/NEDD8, associates with cullin, and is functionally conserved. DCN1 activity is required for pollen development transitions and embryogenesis, and for pollen tube growth. Plant proteomes show remarkable plasticity in reaction to environmental challenges and during developmental transitions. Som...

Controlling variations in plasma membrane (PM) protein abundance is of utmost importance for development in higher plants. For modulating PM protein activity, endocytosed proteins can be either cycled between PM and endosomes or sorted for their irreversible inactivation to lysosomes/vacuoles. Cargo ubiquitination triggers vacuolar delivery for deg...

Recent years have provided us with spectacular insights into the biology of the plant hormone auxin, leaving the impression of a highly versatile molecule involved in virtually every aspect of plant development. A combination of genetics, biochemistry, and cell biology has established auxin signaling pathways, leading to the identification of two d...

Plant tropisms are decisively influenced by dynamic adjustments in spatiotemporal distribution of the growth regulators auxin. Polar auxin transport requires activity of PIN-type auxin carrier proteins, with their distribution at the plasma membrane significantly contributing to the directionality of auxin flow. Control of PIN protein distribution...

Cross-talk between plant cells and their surroundings requires tight regulation of information exchange at the plasma membrane (PM), which involves dynamic adjustments of PM protein localization and turnover to modulate signal perception and solute transport at the interface between cells and their surroundings. In animals and fungi, turnover of PM...

Plant proteomes show remarkable plasticity in reaction to environmental challenges and during developmental transitions. Some of this adaptability comes from ubiquitin-mediated protein destruction regulated by cullin-RING E3 ubiquitin ligases (CRLs). CRLs are activated through modification of the cullin subunit with the ubiquitin-like protein RUB/N...