Katarzyna Glowacka

Katarzyna Glowacka
University of Nebraska at Lincoln | NU · Department of Biochemistry

PhD

About

63
Publications
12,899
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,793
Citations
Additional affiliations
August 2018 - present
University of Nebraska at Lincoln
Position
  • Professor (Assistant)
November 2017 - August 2018
University of Illinois, Urbana-Champaign
Position
  • Researcher
July 2014 - October 2017
University of Illinois, Urbana-Champaign
Position
  • PostDoc Position
Description
  • Understanding low-temperature limits of Miscanthus and its photosynthetic variation and basis
Education
July 2003 - July 2008
Adam Mickiewicz University
Field of study
  • Biology
October 2001 - July 2003
Adam Mickiewicz University
Field of study
  • Experimental Biology
October 1998 - July 2001
Adam Mickiewicz University
Field of study
  • Biology

Publications

Publications (63)
Article
Water deficit currently acts as one of the largest limiting factors for agricultural productivity worldwide. Additionally, limitation by water scarcity is projected to continue in the future with the further onset of effects of global climate change. As a result, it is critical to develop or breed for crops that have increased water-use efficiency...
Preprint
Full-text available
Arabidopsis thaliana has a wide elevational range and much of its diversity may be associated with local adaptation to elevation. We took a multi-regional view of the genomics and physiology of elevational adaptation in Arabidopsis, with >200 ecotypes, including 17 newly collected from Africa. We measured plant responses to potential high elevation...
Article
Full-text available
Little is known about the interactive effects of exogenous nitric oxide (NO) and abscisic acid (ABA) on nitrogen (N) metabolism and related changes at molecular and biochemical levels under drought stress. The present study highlights the independent and combined effect of NO and ABA (grouped as “nitrate agonists”) on expression profiles of represe...
Article
Full-text available
Miscanthus is a perennial wild grass that is of global importance for paper production, roofing, horticultural plantings, and an emerging highly productive temperate biomass crop. We report a chromosome-scale assembly of the paleotetraploid M. sinensis genome, providing a resource for Miscanthus that links its chromosomes to the related diploid Sor...
Article
Full-text available
Miscanthus is a perennial grass with potential for lignocellulosic ethanol production. To ensure its utility for this purpose, breeding efforts should focus on increasing genetic diversity of the nothospecies Miscanthus × giganteus (M×g) beyond the single clone used in many programs. Germplasm from the corresponding parental species M. sinensis (Ms...
Article
Miscanthus is a perennial wild grass that is of global importance for paper production, roofing, horticultural plantings, and an emerging highly productive temperate biomass crop. We report a chromosome-scale assembly of the paleotetraploid M. sinensis genome, providing a resource for Miscanthus that links its chromosomes to the related diploid Sor...
Article
Full-text available
Improving photosynthetic efficiency is widely regarded as a major route to achieving much-needed yield gains in crop plants. In plants with C3 photosynthesis, increasing the diffusion conductance for CO2 transfer from substomatal cavity to chloroplast stroma (gm) could help to improve the efficiencies of CO2 assimilation and photosynthetic water us...
Article
Full-text available
To breed improved biomass cultivars of Miscanthus ×giganteus, it will be necessary to select the highest‐yielding and best‐adapted genotypes of its parental species, M. sinensis and M. sacchariflorus. We phenotyped a diverse clonally‐propagated panel of 569 M. sinensis and nine natural diploid M. ×giganteus at one subtropical (Zhuji, China) and fiv...
Article
Full-text available
Overwintering ability is an important selection criterion for Miscanthus breeding in temperate regions. Insufficient overwintering ability of the currently leading Miscanthus biomass cultivar, M. ×giganteus (M×g) ‘1993‐1780’, in regions where average annual minimum temperatures are ‐26.1 °C (USDA hardiness zone 5) or lower poses a pressing need to...
Article
Full-text available
To improve the efficiency of breeding of Miscanthus for biomass yield, there is a need to develop genomics‐assisted selection for this long‐lived perennial crop by relating genotype to phenotype and breeding value across a broad range of environments. We present the first genome‐wide association (GWA) and genomic prediction study of Miscanthus that...
Article
Full-text available
Prediction of stomatal conductance is a key element to relate and scale up leaf-level gas exchange processes to canopy, ecosystem and land surface models. The empirical models that are typically employed for this purpose are simple and elegant formulations which relate stomatal conductance on a leaf area basis to the net rate of CO2 assimilation, h...
Poster
Full-text available
The aim of this work is to engineer energycane and Miscanthus to produce an abundance of natural oil that can be converted into biodiesel, biojet fuel, and bioproducts. Abstract: The Miscanthus x giganteus has proved a highly productive and cold tolerant C4 biomass feedstock. As a sterile interspecific hybrid, it lacks genetic variation. Bioenginee...
Article
Full-text available
Insufficient water availability for crop production is a mounting barrier to achieving the 70% increase in food production that will be needed by 2050. One solution is to develop crops that require less water per unit mass of production. Water vapor transpires from leaves through stomata, which also facilitate the influx of CO2during photosynthetic...
Article
Full-text available
Spring drought can adversely affect the productivity of barley (Hordeum vulgare L.) by reducing the yield. Because seed osmopriming can enhance crop productivity, we examined the potential of CaCl2 treatment to improve drought tolerance in spring barley. Initially, we applied the priming procedure (5, 50, and 500 mM) to caryopses and assessed its e...
Article
Full-text available
Crop leaves in full sunlight dissipate damaging excess absorbed light energy as heat. When sunlit leaves are shaded by clouds or other leaves, this protective dissipation continues for many minutes and reduces photosynthesis. Calculations have shown that this could cost field crops up to 20% of their potential yield. Here, we describe the bioengine...
Article
Full-text available
Stable transformation of plants is a powerful tool for hypothesis testing. A rapid and reliable evaluation method of the transgenic allele for copy number and homozygosity is vital in analyzing these transformations. Here the suitability of Southern blot analysis, thermal asymmetric interlaced (TAIL-)PCR, quantitative (q)PCR and digital droplet (dd...
Article
Full-text available
sThe goal of this study was to investigate if chilling tolerance of C4 photosynthesis in Miscanthus can be transferred to sugarcane by hybridization. Net leaf CO2 uptake (Asat) and the maximum operating efficiency of photosystem II (ФPSII) were measured in warm conditions (25 °C/20 °C), and then during and following a chilling treatment of 10 °C/5...
Article
Full-text available
A clone of the hybrid perennial C4 grass Miscanthus × giganteus (Mxg) is known for achieving exceptionally high rates of leaf CO2 uptake during chilling. This is a requisite of success in the early spring, as is the ability of the leaves to survive occasional frosts. The aim of this study was to search for genotypes with greater potential than Mxg...
Article
Full-text available
The goal of this study was to identify cold-tolerant genotypes within two species of Miscanthus related to the exceptionally chilling-tolerant C4 biomass crop accession: M. ×giganteus ‘Illinois’ (Mxg) as well as in other Mxg genotypes. The ratio of leaf elongation at 10 °C/5 °C to that at 25 °C/25 °C was used to identify initially the 13 most promi...
Article
Changes in physiological parameters (relative water content (RWC), biomass, water use efficiency (WUE), net photosynthetic yield (PN) and quantum yield of PSII (Fv/Fm)), in proline and sugar content, and expression profile of genes reported to be associated with the barley response to water deficit, including LEA genes, NHX1, Hsdr4, BLT101 and gene...
Article
Full-text available
Background and Aims Miscanthus is a perennial C4 grass that is a leading potential feedstock crop for the emerging bioenergy industry in North America, Europe and China. However, only a single, sterile genotype of M. × giganteus (M×g), a nothospecies derived from diploid M. sinensis (Msi) and tetraploid M. sacchariflorus (Msa), is currently availab...
Article
Miscanthus ×giganteus (Mxg) is an important bioenergy feedstock crop, however genetic diversity among legacy cultivars may be severely constrained. Only one introduction from Japan to Denmark of this sterile, triploid, vegetatively propagated crop was recorded in the 1930's. We sought to determine if the Mxg cultivars in North America were all syno...
Article
Full-text available
The influence of gas exchange parameters on yields of six Miscanthus clones were evaluated in two locations over three years. Five out of six genotypes had significant increases in the photosynthetic rate and intrinsic water use efficiency between the third and first or third and second years, while intercellular CO2 concentration and stomatal cond...
Conference Paper
Miscanthus ×giganteus (Mxg) is a high-biomass producing perennial grass that is a hybrid between M. sinensis (Msi) and M. sacchariflorus (Msa). Numerous clones of sterile triploid Mxg have been named in Europe and North America, but it is unclear how much genetic diversity is present among them. Mxg was introduced to Denmark from Japan in 1935 and...
Article
Full-text available
Miscanthus spp. is a valuable energy grass with a high biomass production, making it a natural source for first- and second-generation fuels. Therefore, the aim of this paper was to investigate a methodology for the haploid production of diploid Miscanthus sinensis Andersson by anther culture. In three experiments the effect of genotype, induction...
Article
Full-text available
Miscanthus (subtribe Saccharinae, tribe Andropogoneae, family Poaceae) is a genus of temperate perennial C4 grasses whose high biomass production makes it, along with its close relatives sugarcane and sorghum, attractive as a biofuel feedstock. The base chromosome number of Miscanthus (x = 19) is different from that of other Saccharinae and approxi...
Data
Table S3. GoldenGate OPA containing the probe details for every marker, as provided by Illumina.
Data
Figure S2. Different amplicon profiles seen in the fragment analysis of SSR markers. The length of the amplicon, in bp, is shown on the horizontal axis and the fluorescence intensity on the vertical axis. Several profiles show "stutter peaks" that are associated with a main peak. These are not counted as distinct marker states.
Data
Table S5. Markers listed in the order of occurrence on the combined maximum likelihood linkage map, with linkage group and map position in cM.
Data
Full-text available
Figure S5. Linearity of genetic maps for Sorghum bicolor and Miscanthus sinensis. Markers with unique placement in sorghum were assigned map positions by interpolation relative to the map of Mace et al. 2009 [30].
Data
Table S1. Phenotypes of the M.sinensis 'Grosse Fontaine' and M.sinensis 'Undine' parents, as measured in mature plants grown in the greenhouse (Figure 1 panels C).
Data
Fasta file of the M.sinensis RNAseq assembly.
Data
Full-text available
Figure S6. Sequence alignment of two independent regions of Miscanthus PPDK paralogs 1 and 2 illustrating indels and SNV used as molecular markers to place C4-PPDK on the linkage map. A) 129 base pairs of sequence from PPDK paralog 1. The G/A single nucleotide polymorphism was converted into the CAPS marker, EBI 847. B) 129 base pairs of PPDK paral...
Data
Table S4. File containing the primer details for all the SSRs.
Data
Table S2. RNA sequencing and assembly data table.
Data
Figure S1. Distribution of sorghum gene models (top stripe, green), Grosse Fontaine and Undine RNAseq reads (middle stripe, depth in log scale Blue-Orange-Green), and genotyped SNVs (bottom stripe, red) along the ten Sorghum bicolor chromosomes. Each chromosome shown to scale (length in Mb shown to left).
Data
Table S7. Details of revised linkage groups 16 and 17 including two C4-PPDK markers.
Data
Full-text available
Figure S3. Colinearity dot plots of the two mapping methods, maximum likelihood and regression (Kosambi).
Data
Full-text available
Figure S4. Colinearity dot plots of the Grosse Fontaine maps versus the Undine maps made using the maximum likelihood (top) and regression algorithms (bottom).
Data
Table S6. Genotype scores and marker statistics including segregation distortion for markers and mapping statistics for the integrated map shown in Figure 3.
Article
This study presents the results of investigations of variation, genotype × year interactions and genotype × year × location interactions for the yield and morphological traits of several selected clones of energy grasses of the genus Miscanthus. The analyses were performed on the best clones of selected hybrid plants, which were obtained within the...
Article
Grasses from the genus Miscanthus are becoming a key renewable raw material for energy production. The aim of this study was to find a suitable treatment combination that would effectively induce polyploids using in vitro shoots propagated from Miscanthus grass species. The experiments had a three-factorial arrangement (genotype, colchicine concent...
Article
Full-text available
Several grass species of the genus Miscanthus are considered to be outstanding candidates for a sustainable production of biomass to generate renewable energy. The purpose of this study was to investigate the effects of genotype, the developmental stage of the explant donor inflorescence and the induction medium on the success rate of micropropagat...
Article
Full-text available
Grasses from the genus Miscanthus have several characteristics that make them very favourable crops for efficient, low input, multifunctional and environmentally friendly biomass production. This study is aimed to improve a polyploidisation method to effectively induce polyploids in Miscanthus sinensis and Miscanthus x giganteus. Colchicine was app...
Article
Full-text available
Miscanthus sinensis is a promising species for biomass production. Influences of genetic and nongenetic factors on androgenesis induction efficiency were investigated. This is the first report on successful induction of pollen-derived callus in M. sinensis. The callus yield was strongly affected by genotype. A beneficial influence of cold pretreatm...
Article
The purpose of this study was to find a suitable treatment combination that would effectively induce polyploids in the grass species from the genus Miscanthus. In the experiments, the combinations of two concentrations of colchicine, three options of supplementing the colchicine solution with additional substances and three exposure times were test...
Article
The study presents the results of genetic analysis of selected morphological and physical stem characteristics determining lodging resistance in doubled haploid (DH) lines of spring barley produced from F1 hybrids of the six-rowed variety Klimek with the two-rowed variety Maresi. The DH lines, the initial forms, and F1 and F2 hybrids were tested in...

Projects

Project (1)
Project
The RIPE project is investigating six unique approaches to improving photosynthesis in order to boost crop yields to the necessary new extremes. We’ve started by engineering improved photosynthetic efficiency in silico, and are working to determine how molecular changes are affecting the process, and will later be confirming increased yields in the field environment.