Katarína Ražná

Slovak University of Agriculture in Nitra - Slovenska posnohospodarska univerzita v Nitre · Department of Genetics and Plant Breeding (KGŠR)

Lavandula species are one of the most useful aromatic and medicinal plants and have great economic potential. The phytopharmaceutical contribution of the secondary metabolites of the species is unquestionable. Most recent studies have been focusing on the elucidation of the genetic background of secondary metabolite production in lavender species....

Drought susceptible (Aladin, Dagmar) and drought tolerant (Seladon, Venturero) varieties of summer wheat were examined in experiment. Genotypes were tested under In vitro conditions on Murashige-Skoog culture medium with different concentrations (0, 5, 10, 15 and 20 %) of polyethylene glycol to induce dehydration stress conditions. Genomic response...

Aesculus hippocastanum L. ˈBaumaniiˈ (syn.: A. hippocastanum L. ˈFlore Plenoˈ; A. hippocastanum ˈPlenaˈ) is a rarely cultivated cultivar characterized by fully white and sterile flowers, and as a result it does not produce fruit. For identification and characterization of the morphological, molecular, and biochemical parameters of this cultivar, we...

Some seeds of flowering plants can differentiate their seed coat epidermis into the specialized cell layer producing a hydrophilic mucilage with several ecological functions, such as seed hydration, protection, spatial fixation, stimulation of metabolic activity and development of seed. Due to the species- and genotype-dependent variabilities in th...

Lignans, as secondary metabolites synthesized within a phenylpropanoid pathway, play various roles in plants, including their involvement in growth and plant defense processes. The health and nutritional benefits of lignans are unquestionable, and many studies have been devoted to these attributes. Although the regulatory role of miRNAs in the bios...

Gladiolus as economically important flowering plants reflect the wide range of morphological variability of the inflorescences. The aim of current gladiolus breeders is to create interesting new genotypes by different breeding techniques, carrying a variety of colour, size, texture and shapes of flowers and inflorescences. Despite the well-establis...

The significant healing effect of Tilia platyphyllos Scop. and Tilia cordata Mill. flowers are well known. However, the flowers of Tilia tomentosa Moench. are not suitable for harvest due to their toxic effects. To investigate the diverse background of this effect, we applied a functional miRNA-based marker, mannose expression analysis and determin...

Naturally occurring lignans are present in seeds, nuts, cereals, vegetables, and fruits. Lignans play various roles in plants and their multipurpose functions of different organisms including humans is considerable. They are involved in plant defence mechanisms through their antioxidant, antiviral, antibacterial, and antifungal properties. The lign...

Pollen allergies are an important and relevant question in the personal life of millions of people. They are well characterized on the genomic and proteomic level of allergens per se and on medicinal level, but the very few information exists about the natural variability and background of pollen allergen transcription patterns. Here, the variabili...

Recent studies reported a significant contribution of microRNAs (miRNAs) in plant response to elicitors. The aim of our study was to analyse the effect of exogenously applied plant-derived elicitor cobalt-diglycyrrhizinate on Triticum aestivum L. genome based on the functional characterization of stress associated miRNAs. Our results confirmed the...

The aim of this study was to characterize extracts from the leaves of Ginkgo biloba L. from selected Slovakian localities in terms of the content of bioactive constituents, antioxidants and their antimicrobial properties. The results indicated that the content of antioxidants was sample-specific, and this specificity was statistically significant....

RNA interference is a known phenomenon of plant immune responses, involving the regulation of gene expression. The key components triggering the silencing of targeted sequences are double-stranded RNA molecules. The regulation of host–pathogen interactions is controlled by miRNA molecules, which regulate the expression of host resistance genes or t...

Ginkgo biloba L. is characterized by its high level of resistance to climatic conditions, diseases, and pests. In Slovakia, there is a rich collection of genetic resources of ginkgo consisting of 288 trees growing in 103 locations and providing valuable biological material for scientific research. There have been documented 45 trees of ginkgo older...

Inter primer binding site (iPBS) polymorphism was investigated for common ivy ( Hedera helix , L.) to obtain the knowledge on genetic diversity in this species. Actually, a very limited information exists about application of DNA markers in Hedera helix . Natural and planted European populations of ivy were analysed using an iPBS marker 5′ACCTGGCGT...

Wheat is considered to be the world oldest and most widely grown plant. Current genetic and genomic information about wheat is extensive and contains partial knowledge of various types of markers up to the genomic sequence data. Up to date, several different types of DNA markers have been used in the mapping of the wheat genome, that were used to d...

The aim of this thesis was to find the content of rosmarinic acid (RA) in the selected varieties of sage (Salvia officinalis L.): Krajová, Purpurascens, Berggarten, Tricolor, Icterina and Alba. In the monitoring years 2013-2015 and two collection dates was found that the highest RA content was in Tricolor in 2013 (30,084.65 mg*kg-1) collected in th...

Ginkgo biloba, the last extant representative of a lineage of Mesozoic gymnosperms, is one of the few seed plants with an exceptionally long (~300 Myr) evolutionary history free of genome-wide duplications (polyploidy). Despite this genome conservatism, we have recently found a viable spontaneous tetraploid Ginkgo sapling during routine screening o...

Ginkgo biloba, the last extant representative of a lineage of Mesozoic gymnosperms, is one of the few seed plants with an exceptionally long (~300 Myr) evolutionary history free of genome-wide duplications (polyploidy). Despite this genome conservatism, we have recently found a viable spontaneous tetraploid Ginkgo sapling during routine screening o...

Bunium persicum Boiss. seeds have been used for medicinal and nutritional properties such as antioxidant, antihelmetic and antimicrobial activity. The aim of this study was to to tested protective role of cumin extract against abiotic stress by microRNA markers. Secondary also was to evaluate antioxidant activity as well as total polyphenol, flavon...

The subject of the research was to find out the cultural expansion of two species of Taxodium, namely Taxodium ascendens (Brongn.) and Taxodium distichum (L.) L. C. Rich. The occurrence of individuals was recorded on 18 different localities of Slovakia, obtaining the taxonomic-dendrometric characteristics of individual trees. Appropriate methodolog...

The most suitable method of total DNA extraction still remains the crucial step for many plant species, although there are many different protocols and commercial kits for DNA isolation. In this study, five different extraction protocols were analysed to find out the most appropriate method for DNA extraction from Hedera helix L. This species has n...

Milk thistle can be an annual, rarely a biennial medicinal plant. Polyfactorial field experiment was established during the vegetation period of the years 2012–2015. Three evaluated factors were as follows: crop residues – intercrop – fertilization. Milk thistle was integrated to four crop rotation design with following order of crops: 1. common pe...

MicroRNAs represent small non-coding RNAs that play important role in regulating gene expression under various biotic and abiotic stresses as well as in different developmental stages of plants. They are involved in wide variety of biological and metabolic pathways of plants. The research was focused on the potential of selected miRNA-based molecul...

MicroRNAs (miRNAs) are a class of 19 - 24 nucleotide long non-coding RNAs derived from hairpin precursors, regulating various biological, metabolic and developmental processes at the post-transcriptional level. Many of the known miRNAs are evolutionary conserved across diverse plant species and function in the regulatory control of fundamentally im...

The first study of the pattern of dLUTE insertions as detected by PCR length polymorphism technique was performed. In silico approach was used for developing the dLUTE based marker system. Alignment analysis were performed to find a region that is dLUTE specific and primers designed withing this region not corresponding to any of sequences of L-gen...

MicroRNAs (miRNAs) are a class of non-coding RNAs about 20-24 nucleotides long. They play an important role in the gene regulation at the post-transcriptional level. They affect the plant genome response to environmental stress. The miRNA-based molecular markers is type of functional markers reported in very few plants. However, the information con...

RAPD and ISSR markers were used to analyse intra and inter - specific variability of 16 A. caudatus, 18 A. cruentus and 21 A. hypochondriacus accessions. The potential of both approaches was evaluated using three random and three microsatellite primers amplifying in total of 1126 (RAPD), respectively 1013 (ISSR) scorable fragments. Similarity valu...

Flax (Linum usitatissimum L.) is one of the oldest domesticated plants — it was cultivated as early as in ancient Egypt and Samaria 10,000 years ago to serve as a source of fiber and oil, whence it later spread around the world. Compared with other plants, the flax genome consists of a high number of repetitive sequences, middle repetitive sequence...

Corylus avellana is the source of inhalant allergies induced by hazel pollen as well as food allergies induced after ingestion of hazelnuts. In this study, real-time PCR approach was used to analyse expression of hazel pollen allergens on the molecular level. Relative quantity of hazelnut allergens Corylus avellana, L. CorA and Corylus avellana, L....

The increase interest and study of underutilized and neglected crops is actual for the last years. In the study, the restriction polymorphisms of GBSSI and SSSI gene in gamma-irradiated mutant lines of amaranth was performed. To evaluate polymorphisms of restriction sites endonucleases AciI, BsaJI, FatI EcoRI, BamHI, PstI, HpaII and PciI were used....

To determine which of several gamma-irradiated mutants of amaranth Ficha cultivar and K-433 hybrid are most genetically similar to their non-irradiated control genotypes, we performed amplified fragment length polymorphism (AFLP) based analysis. A total of 40 selective primer combinations were used in reported analyses. First analyses of gamma-irra...

ISSR markers were used to analyse gamma-irradiated mutants of amaranth Ficha cultivar and K-433 hybrid; a total of six microsatellite - primed PCR markers were used in reported analyses. First analyses of gamma-irradiated amaranth mutant lines were done used the ISSR. In the study, primers that distinguish the mutant lines from control plant are re...

The aim of the study was to determinate the level of expression of silver birch allergen Betv1 in pollen samples from different Ukraine areas by RT-qPCR SYBR Green assay. Protocol for quantifying the expression of Betv1 allergen was developed when testing of three housekeeping genes-cyclophylin, alpha-tubulin and transcription factor CBF1. Samples...

Maintaining genetic diversity for crop improvement and improving the quality of genetic resource management is both an inevitable part of nowadays maize breeding. ISSR markers brings the potential of finding a marker system to discriminate Iowa Stiff Stalk Synthetic and Iodent Reid populations of Zea mays, L. and on the base on coefficients of gene...

As plums posses wide diversity range, findings of effective marker systems for their differentiation is still actual. IRAP markers derived from Cassandra retrotransposon were applied to distinguish local Slovak plums when compared them to european standart plum cultivars. Cass1primer created totally ten different levels and Cass2 created eighteen d...

Sodium Azide Induced Morphological and Molecular Changes in Persimmon ( Diospyros Lotus L.) Persimmon Diospyros lotus L. is an underutilized species, used as rootstock for Japanese persimmon ( Diospyros kaki Thunb.) in many European countries. It has a potential for ornamental uses due to its diploid genome that suggests an easy mutation induction....

Amaranth is one of the re-discovered crops. Amaranth DNA polymorphism changes caused by γ-radiation in selected amaranth lines -Amaranthus cruenthus L. were observed using genotypes Ficha and Hybrid K-433 (the result of interspecific hybridization of species A. hypochondriacus  A. hybridus). Mutant lines are M8 generation positively selected plant...