Kassandra Groux

Kassandra Groux
École Supérieure de Physique et de Chimie Industrielles | ESPCI · Langevin Institute

Master of Engineering

About

27
Publications
2,649
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137
Citations
Introduction
Imaging of intracellular dynamics of retinal cell cultures with Dynamic Full-Field OCT to model degenerative diseases

Publications

Publications (27)
Article
Full-text available
Retinal degenerative diseases lead to the blindness of millions of people around the world. In case of age-related macular degeneration (AMD), the atrophy of retinal pigment epithelium (RPE) precedes neural dystrophy. But as crucial as understanding both healthy and pathological RPE cell physiology is for those diseases, no current technique allows...
Article
Eye movements are commonly seen as an obstacle to high-resolution ophthalmic imaging. In this context we study the natural axial movements of the in vivo human eye and show that they can be used to modulate the optical phase and retrieve tomographic images via time-domain full-field optical coherence tomography (TD-FF-OCT). This approach opens a pa...
Thesis
This thesis aims to demonstrate the usefulness of static and dynamic Full-Field Optical Coherence Tomography (FFOCT) for retinal imaging, with in vitro (cell cultures in 2D or 3D), ex vivo (retinas dissected from different species) and in vivo (direct imaging of the eye of the patient) samples.By stabilising the three dimensional and timelapse dyna...
Preprint
Eye movements are commonly seen as an obstacle to high-resolution ophthalmic imaging. In this context we study the natural axial movements of the in vivo human eye and show that they can be used to modulate the optical phase and retrieve tomographic images via time-domain full-field optical coherence tomography (TD-FF-OCT). This approach opens a pa...
Preprint
Full-text available
Retinal degenerative diseases lead to the blindness of millions of people around the world. In case of age-related macular degeneration (AMD), the atrophy of retinal pigment epithelium (RPE) precedes neural dystrophy. But as crucial as understanding both healthy and pathological RPE cell physiology is for those diseases, no current technique allows...
Article
Full-text available
We report on a theoretical model for image formation in full-field optical coherence tomography (FFOCT). Because the spatial incoherence of the illumination acts as a virtual confocal pinhole in FFOCT, its imaging performance is equivalent to a scanning time-gated coherent confocal microscope. In agreement with optical experiments enabling a precis...
Preprint
Full-text available
We report on a theoretical model for image formation in full-field optical coherence tomography (FFOCT). Because the spatial incoherence of the illumination acts as a virtual confocal pinhole in FFOCT, its imaging performance is equivalent to a scanning time-gated coherent confocal microscope. In agreement with optical experiments enabling a precis...
Article
Full-text available
The highest three-dimensional (3D) resolution possible in in vivo retinal imaging is achieved by combining optical coherence tomography (OCT) and adaptive optics. However, this combination brings important limitations, such as small field-of-view and complex, cumbersome systems , preventing so far the translation of this technology from the researc...
Article
Full-text available
Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal organoids. Coloured images with an endogenous contr...
Article
Full-text available
Allying high-resolution with a large field-of-view (FOV) is of great importance in the fields of biology and medicine, but it is particularly challenging when imaging non-flat living samples such as the human retina. Indeed, high-resolution is normally achieved with adaptive optics (AO) and scanning methods, which considerably reduce the useful FOV...
Preprint
Full-text available
The highest three-dimensional (3D) resolution possible in in-vivo retinal imaging is achieved by combining optical coherence tomography (OCT) and adaptive optics (AO). However, this combination brings important limitations, such as small field-of-view and complex, cumbersome systems, preventing so far the translation of this technology from the res...
Conference Paper
Dynamic FFOCT allows us to see the sub-cellular motion of biological samples. We are able to follow the evolution in the same plane of biological samples for hours thanks to an autofocus procedure. RPE cells are involved in the integrity of retina and vision. We performed a linear scratch assay in RPE cell culture with a surgical scalpel blade, ind...
Conference Paper
We present a filtering procedure based on singular value decomposition to remove artifacts arising from sample motion during dynamic full field OCT acquisitions. The presented method succeeded in removing artifacts created by environmental noise from data acquired in a clinical setting, including in vivo data. Moreover, we report on a new method ba...
Conference Paper
To achieve 3D high-cellular resolution, a great effort, in the past years, was made to develop Adaptive Optics (AO)-OCT systems. However, such systems require quite complex, expensive and cumbersome hardware, making clinical transfer challenging. Moreover, conventional AO correction is limited to the retina isoplanatic patch (about 1deg), reducing...
Conference Paper
Full-text available
To achieve 3D high-cellular resolution, a great effort, in the past years, was made to develop Adaptive Optics (AO)-OCT systems. However, such systems require quite complex, expensive and cumbersome hardware, making clinical transfer challenging. Recently, we demonstrated that the use of spatially incoherent illumination in Time-Domain Full-Field (...
Preprint
Full-text available
Allying high-resolution with a large field-of-view (FOV) is of great importance in the fields of biology and medicine, but particularly challenging when imaging non-flat living samples such as the human retina. Indeed, high-resolution is normally achieved with adaptive optics (AO) and scanning methods, which considerably reduce the useful FOV and i...
Article
Full-text available
Time-domain full-field OCT (FF-OCT) represents an imaging modality capable of recording high-speed en-face sections of a sample at a given depth. One of the biggest challenges to transfer this technique to image in-vivo human retina is the presence of continuous involuntary head and eye axial motion during image acquisition. In this paper, we demon...
Preprint
Full-text available
Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue, but lacks functional information. We present dynamic full-field optical coherence tomography to image living human induced pluripotent stem cell-derived retinal organoids non-invasively. Colored images with an endogenous contrast linked to...
Article
Full-text available
We describe recent technological progress in multimodal en face full-field optical coherence tomography that has allowed detection of slow and fast dynamic processes in the eye. We show that by combining static, dynamic and fluorescence contrasts we can achieve label-free high-resolution imaging of the retina and anterior eye with temporal resoluti...

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Projects

Projects (2)
Project
Image live organoids, cell cultures and fresh excised tissues. Goal: bring D-FFOCT in vivo for retinal imaging with cellular resolution.
Project
3D high-resolution retinal imaging is achieved with SD-AO-OCT, a point-by-point scanning imaging modality. It comes with drawbacks as increased complexity, cumbersome, long acquisition time and high cost, making it hardly transferable into clinics. The objective of this project is to use the fact that FF-OCT is a plane-by-plane imaging modality presenting a low sensitivity to low-order ocular aberrations in order to achieve 3D high-resolution retinal imaging with a simple and compact optical design and ready for clinical use.