Karthik Loganathan

Salem microbes private limited · Synthetic biology

Genome editing technologies have been proven to be an important strategy for drug discovery in the recent years. Among them, clustered regularly interspaced short palindromic repeats (CRISPR) and associated proteins (CRISPR/Cas) is an efficient genome editing tool, which helps mainly in gene regulation and activation of biosynthetic gene clusters (...

Direct cloning of biosynthetic gene clusters (BGCs) from microbial genomes facilitates natural product-based drug discovery. Here, by combining Cas12a and the advanced features of bacterial artificial chromosome library construction, we developed a fast yet efficient in vitro platform for directly capturing large BGCs, named CAT-FISHING (CRISPR/Cas...

The era of nanotechnology has led to the development of the science of miniaturized medicines which has helped the human individual to great extent. The presence of the nanomedicine in the scientific world has steered the human populace to a better life with an advent of precision medicines. In this, the glyconanotechnology has been playing a pivot...

The “postgenomic era” will be dominated by long-read technologies like Oxford Nanopore technology and Pacific Biosystem’s single-molecule real-time sequencing technology. The nanopore technology can produce reads up to 2.3 Mbp length. So technically it can sequence approximately one-third of a Streptomyces genome in a single read unlike other seque...

From time immemorial, natural products (NPs) are a major source of drugs in the pharmaceutical industry ranging from antibiotics, anti-depressants, anti-inflammatory, anti-tumor, and anti-cancer agents to name a few. Actinobacteria and their biosynthetic pathways contribute substantially to these drugs present in the laboratory level, clinical phas...

Actinobacteria are widely distributed in nature, such as marine, soil, and endophytic. Secondary metabolites derived from actinobacteria are valuable for drug discovery and development (R & D). Besides anti-infectious effects, anticancer, immunosuppressive effects, and other effects are displayed by this kind of metabolites. Streptomyces species ar...

Actinobacteria are a potent source of secondary metabolites. These metabolites have unique metabolic activities which make them an important component in the development of a drug. Sesquiterpene belongs to the class of terpenes and are large structurally diverse group of marine natural products with C-15 carbon skeleton. Whole genome sequence infor...

Aim: Coronavirus disease 2019 (COVID-19) caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), attacking mainly on the immune system of a body. This has spread mortality and morbidity all over the world. During this dreadful situation there is an urgent need for the development and rapid dissemination of COVID-19 treatment. Si...

Fungal terpenoids catalyzed by bifunctional terpene synthases (BFTSs) possess interesting bioactive and chemical properties. In this study, an integrated approach of genome mining, heterologous expression, and in vitro enzymatic activity assay was used, and these identified a unique BFTS sub-clade critical to the formation of a 5-15 trans-fused bic...

The research article presents the synthesis, characterization, and biological evaluation of amoxicillin functionalized with AgNPs with low toxicity and good antimicrobial property which can be produced with silver nitrate and amoxicillin drug in an environmentally friendly method. The synthesized nanoparticles were subjected to characterization by...

The marine environment is the most biologically and chemically diverse habitat on Earth, and provides numerous marine-derived products, including enzymes and molecules, for industrial and pharmaceutical applications. Marine biotechnology provides important biological resources from marine habitat conservation to applied science. In recent years, ad...

Nanomedicine is the field of science that deals with organic applications of medicine at the nanoscale level. It primarily addresses finding, anticipating, and treating sickness, as well as using nanotechnology to assist in controlling human frameworks at the cellular level. The nature of nanotechnology allows it to address numerous medical issues...

Two pairs of dibenzospiroketal racemates, (±)-epicospirocin A (1a/1b), (±)-1-epi-epicospirocin A (2a/2b), two (+)-enantiomers of aspermicrones, ent-aspermicrone B (3b) and ent-aspermicrone C (4b), together with two hemiacetal epimeric mixtures, epicospirocin B/1-epi-epicospirocin B (5/6) and epicospirocin C/1-epi-epicospirocin C (7/8) were investig...

Directly cloning of biosynthetic gene clusters (BGCs) from even unculturable microbial genomes revolutionized nature products-based drug discovery. However, it is still very challenging to efficiently cloning, for example, the large (e.g. > 80kb) BGCs, especially for samples with high GC content in Streptomyces. In this study, by combining the adva...

Background Siddha Medicine is a valuable therapeutic choice which is classically used for treating viral respiratory infections, this principle of medicine is proven to contain antiviral compounds. Objective The study is aimed to execute the In Silico computational studies of Phytoconstituents of Siddha Official Formulation Kabasura Kudineer and N...

Nanotechnology has risen as a fascinated research zone, which opens the brilliant entryways of chances that cannot be accomplished in a bulk state. The entrancing universe of nanotechnology shows remarkable nanoparticles with a fascinating properties and applications, which has brought about increasing critical consideration among the analysts. Sin...

Nanoparticles possess fascinating properties and applications, and there has been increasing critical consideration of their use. Because carbon is a component with immaterial cytotoxicity and extensive biocompatibility with different components, carbon nanomaterials have a wide scope of potential uses. Carbon nanodots are a type of carbon nanopart...

Marine microorganisms live in dramatically different environments and have attracted much attention for their structurally unique natural products with potential strong biological activity. Based on the one strain-many compounds (OSMAC) strategy and liquid chromatography mass spectrometry (LC-MS) methods, our continuing efforts on the investigation...

We are reporting the synergistic effects of amoxicillin coated silver nanoparticles, and sodium cholate is used as the stabilizer. Following, the synthesis of AgNPs physicochemical characterization was carried out using UV spectroscopy, XRD, FTIR, FESEM, AFM and NC-AFM analysis. The confirmation of AgNPs was confirmed UV spectral peak at 426 nm, th...

Microbes serve as the most important resource for drug discovery. During our screening for bioactive compounds from our natural products library, a pathogenic fungus, Microdochium majus strain 99049, from wheat was selected for further investigation. A new alkaloid named brocaeloid D (1), together with six previously characterized compounds (2–7) w...

Besides genome editing, CRISPR-Cas12a has recently been used for DNA detection applications with attomolar sensitivity but, to our knowledge, it has not been used for the detection of small molecules. Bacterial allosteric transcription factors (aTFs) have evolved to sense and respond sensitively to a variety of small molecules to benefit bacterial...

Dear Distinguished Professors & Researchers, Greetings from Shri Shakthi Institute of Engineering and Technology (SSIET), Coimbatore, India We would like to inform you with immense pleasure that Department of Biotechnology, SSIET, Coimabtore, India, is hosting a National Level Conference on " MAKE IN INDIA FOR THE BIOTECH WAY FORWARD" in collabo...

Lack of sufficient pure natural compounds hinders further drug developments. The optimization of fermentation conditions is essential to enhance the yield of metabolites. Microbial genome analysis reveals the presence of a large number of cryptic biosynthetic gene clusters, and different strategies are there to trigger these gene pathways for the e...

Sponges and corals are significant sources for marine natural products. They have a pool of novel microorganisms. Due to low cost of gene sequencing, in recent years, several reports are available for novel compounds from sponge- and coral-associated microorganisms. Still, most of the biosynthesis mechanisms are not revealed. There are only few rep...

Sponges and corals are great pool of diverse microbes because they are closely associated with microorganisms that occur either extracellularly or intracellularly. The recent studies have revealed new microbial communities and novel compounds from sponges and corals. Many reports are found toward the availability of antibiotics from the sponge-/cor...

Since the ancient times, microbial diseases remain to be one of the leading causes of mortality worldwide. Historical literature suggests the devastating effects of microbial diseases outbreaks on the socioeconomic of several countries. In such state, discovery and application of vaccines (microbial origin) gave new hope to the mankind to fight mic...

Nonribosomal peptide synthetases (NRPSs) are multi-modular enzymes involved in the biosynthesis of natural products. Bacillamide C was synthesized by Bacillus atrophaeus C89. A nonribosomal peptide synthetase (NRPS) cluster found in the genome of B. atrophaeus C89 was hypothesized to be responsible for the biosynthesis of bacillamide C using alanin...

This book chapter introduces how marine Actinobacteria-inspired nanoparticles are used in the food industry. Food nanotechnology is a zone of rising intrigue, which leads to an entire universe of fresh substantial results for the nourishment and food business. These modules of the nanotechnology application and their functionalities at present led...

The objective of this study was to assess the mosquito controlling potential of aqueous extract of Nerium oleander leaves against the primary and secondary vectors of Japanese encephalitis (Culex tritaeniorhynchus and Cx. gelidus). Phytochemical screening of N. oleander leaves exhibited the presence of saponins, proteins, flavonoids, alkaloids, tan...

Nitrification directly contributes to the ammonia removal in sponges, and it plays an indispensable role in sponge-mediated nitrogen cycle. Previous studies have demonstrated genomic evidences of nitrifying lineages in the sponge Theonella swinhoei. However, little is known about the transcriptional activity of nitrifying community in this sponge....

The present study was carried out to screen the actinobacteria isolated from marine sediments collected from Chennai, Tamilnadu, India for their antibacterial and antioxidant activites. A total of 6 isolates of marine Actinobacteria were isolated and were designated as VSKB 1 to VSKB 6. Out of the six isolates only one isolates VSKB-3 showed activi...

Marine sponges often harbor dense and diverse microbial communities including actinobacteria. To date no comprehensive investigation has been performed on the culturable diversity of the actinomycetes associated with South China Sea sponges. Structurally novel aromatic polyketides were recently discovered from marine sponge-derived Streptomyces and...

This study describes a novel biological route for the biosynthesis of silver oxide nanoparticles utilising the aqueous extract of Callistemon lanceolatus D.C. leaves. Formation of silver oxide nanoparticles was confirmed by UV–visible spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscope–energy dispersive X-ray spectro...

The present study was conducted for the isolation of potential bacteria from the desert soil, their molecular identification and prediction of restriction sites of the potential isolate using the bioinformatics tools. Production of the metabolites was done by inoculating in nutrient broth of pH 8.6. Metabolite was bluish-green in color; it was extr...

The current study was aimed to evaluate phytochemical composition and antioxidant property of red, white and yellow flower yielding varieties of Bougainvillea glabra leaves by various in vitro methods. Antioxidant activity of all the three varieties was analyzed by DPPH radical scavenging assay. Red variety showed highest DPPH radical scavenging ac...

The aim of this study is to analyze the phytochemical composition and antioxidant activity of Ficus elastica (F. elastica). Antioxidant activity of methanolic extract of Ficus elastica leaves was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, total antioxidant activity test, iron chelating activity test and reducing pow...

The study focused on statistical optimization of Nilavembukudineer using RSM and its antibacterial activity. The combined interactive effect of different variables on Nilavembukudineer production was studied by Response Surface Methodology (RSM). The efficiency of the antibacterial compound was tested against clinical isolate of Salmonella typhi. T...

Marine Bacillus species are potent producers of novel enzymes. Marine Bacillus VITRKHB was observed to be efficient for cellulolytic activity. It was employed for the production of extracellular cellulase. Cellulase was partially purified to 1.6-fold in a stepwise manner by ammonium sulfate precipitation, dialysis, and DEAE ion exchange chromatogra...

The present study was focused on screening and characterization of tyrosinase enzyme produced by marine actinobacteria and its application in phenolic compounds removal from aqueous solution. A total of 20 strains were isolated from marine sediment sample and screened for tyrosinase production by using skimmed milk agar medium. Among 20 isolates, t...

Staphyloccus aureus (S. aureus) is a well known human pathogen known to causes a verity of infections in humans. In recent years S. aureus is reported to show drug resistant toward commonly known drugs. Therefore, this study was designed to study the pattern of antibiotic resistance in 50 clinical isolates of S. aureus isolated at Dhanwantri Hospit...

The aim of this present study was to identify the chemical ingredients and evaluations of antibacterial activity of different crude extracts from the locally grown Acacia karoo and Ziziphus mauritiana. The in vitro evaluation of antibacterial activities of different crude extracts was measured by Agar Well Diffusion method. The identification of ch...

Present study is focused on the synthesis of titanium dioxide nanoparticles (TiO2 NPs) from marine actinobacteria and to check their antibiofouling activity against the isolated biofouling bacteria. A total of five actinobacterial strains were isolated from Chennai marine sediments, Tamilnadu, India, which were designated as SV1-SV5. Two biofouling...

The aim of this study was to evaluate the phytochemical composition and antioxidant potential of the Ficus benghalensis (F. benghalensis) by in vitro methods. Methanolic extract of F. benghalensis leaves was evaluated for the presence of carbohydrates, proteins, phenolic compounds, oil and fats, saponins, flavonoids, alkaloids and tannins by using...

Ipomoea fistulosa is an evergreen shrub with heart shaped leaves which is used for the treatment of various diseases in traditional medication. In this study, aqueous extract of I. fistulosa leaves was evaluated for phytochemical composition, antioxidant activity and cytotoxic activity by various in vitro methods. Phytochemical screening of the I....

The present study was focused on isolation, screening, characterization and application of biosurfac-tant producing marine actinobacteria. Twenty actinobac-teria were isolated from marine water sample and were primarily screened for biosurfactant production using hemolytic activity method. Among the 20 isolates, six showed positive result for hemol...

The present study was focused on isolation, screening, characterization and application of biosurfac-tant producing marine actinobacteria. Twenty actinobac-teria were isolated from marine water sample and were primarily screened for biosurfactant production using hemolytic activity method. Among the 20 isolates, six showed positive result for hemol...

The present study was focused on isolation, screening, characterization and application of biosurfactant producing marine actinobacteria. Twenty actinobacteria were isolated from marine water sample and were primarily screened for biosurfactant production using hemolytic activity method. Among the 20 isolates, six showed positive result for hemolyt...

The study was planned to screen the marine actinobacterial extract for the protease inhibitor activity and its anti- Pf activity under in vitro and in vivo conditions. Out of 100 isolates, only 3 isolates exhibited moderate to high protease inhibitor activities on trypsin, chymotrypsin and proteinase K. Based on protease inhibitor activity 3 isolat...

The aim of the present study was to increase the production of antibacterial compound from marine actinobacteria by the effect of chemical mutation as well as optimization of media using Response Surface Methodology (RSM). The efficiency of the antibacterial compound was tested against clinical isolate of Pseudomonas aeruginosa. Mutational effect w...

Elaeocarpus ganitrus Roxb. (E. ganitrus) is an evergreen tree belongs to the family Elaeocarpaceae. E. ganitrus is an important medicinal plant in traditional medication system and mainly used to cure nervous system related disorders. In recent past, E. ganitrus has been extensively studied for its medicinal properties by advance scientific techniq...

The study focused on the combination of marine actinobacterial extract and its mediated zirconium oxide nanoparticles for anti-biofouling application against the isolated biofouling bacteria. Streptomyces sp.VITSDSB was used for the synthesis of zirconium oxide nanoparticles, since it showed the maximum potential for the synthesis of nanoparticles....

The aim of the present study was to isolate microorganism that produces biosurfactant and to optimize its production. Lipase and hemolytic activity were performed as primary screening procedures. Three potential isolates (GMPI to GMP3) were subjected to secondary screening by Drop collapsing method, Oil displacement method and Emulsification index....

Biosurfactant was obtained from Nocardiopsis VITSISB and it was used in cosmetic formulation of toothpaste, replacing Sodium Lauryl Sulphate which is normally used in commercial toothpaste as a surfactant. This biosurfactant toothpaste was qualitatively analyzed by several tests like Spreadability Test, Foaming ability Test, Abrasiveness Test, Brin...

Amylases (EC 3.2.1.1) hydrolyses the α-1,4 glycosidic linkages of starch. Microbes are the most preferred sources of enzymes due to their broad biochemical diversity. In the present study saltpan soils were collected from Ongole, Andhra Pradesh. Twenty bacterial isolates were isolated and screened for amylase production, Four isolates showed amylol...

Present study was focused on purification and activity of amylases of marine eubacteria. Among 23 bacterial isolates from Nicobar Islands, Halobacillus sp amylus was selected and further examined. Enzyme activity was evaluated at different temperatures (25, 30, 35, 40 and 45°C), at varying pH (6.0, 6.5, 7.0, 7.5 and 8.0) and by using various carbon...