Karol Wróblewski

UNIVERSITY OF RZESZÓW · Department of Experimental and Clinical Pharmacology

Citation: Wróblewski, K.; Szultka-Młyńska, M.; Courtney, R.J.; Buszewski, B.; Tutka, P. Development and Validation of LC-MS/MS Method for Determination of Cytisine in Human Serum and Saliva. Int. J. Mol. Sci. 2023, 24, 15364. Abstract: Cytisine (CYT) is a quinolizidine alkaloid used for nicotine addiction treatment. Recent clinical trial data regar...

Carbamazepine (CBZ) is an anticonvulsant drug, widely used also in various disorders like neuropathic pain, bipolar affective disorder, resistant schizophrenia, and trigeminal neuralgia. Appropriate analytical procedures are necessary to monitor, detect and quantify CBZ in pharmaceutical formulations and biological samples. The search for new metho...

Vortioxetine (VOR) is a new antidepressant drug used to treat major depressive disorder. In this work, a novel, simple, rapid, accurate, precise, selective, stability-indicating, and fully validated high-performance liquid chromatography method with diode array detection (HPLC-DAD) was developed to determine VOR in bulk and pharmaceutical formulati...

Quinolizidine alkaloids exhibit various forms of biological activity. A lot of them were found in the Leguminosae family, including Laburnum and Genista. The aim of the study was the optimization of a chromatographic system for the analysis of cytisine and N-methylcytisine in various plant extracts as well as an investigation of the cytotoxic activ...

Background: Determination of psychotropic drugs in clinical study is significant, and the establishment of methodologies for these drugs in biological matrices is essential for patients’ safety. The search for new methods for their detection is one of the most important challenges of modern scientific research. The methods for analyzing of psychotr...

Retention, separation selectivity and system efficiency of selected basic psychotropic drugs (clozapine, aripiprazole, vortioxetine and zolpidem) and drug metabolites (desmethylclozapine, clozapine N-oxide and dehydroaripiprazole) on Hydro RP, Phenyl-Hexyl and Polar RP columns were studied. Mobile phases containing methanol or acetonitrile as organ...

Background: Identification and quantitative determination of cytisine, especially in biological samples and pharmaceutical formulations, is still a difficult analytical task. Cytisine is an alkaloid with a small and very polar molecule. For this reason, it is very weakly retained on reversed phase (RP) stationary phases, such as commonly used alkyl...

Sorbents used in chromatographic analysis of active compounds are usually silica-based materials with ligands of different origin. Unfortunately residual silanols play also role in mechanisms of analytes’ separation. It often causes analytical problems because generates incorrect peak shapes and poor system efficiency and influences negatively sele...

A simple and sensitive high-performance liquid chromatographic (HPLC) method with diode array detection (DAD) is described for the quantification of some psychotropic drugs: Fluoxetine, Sertraline, Alprazolam, Perphenazine, Zolpidem and Hydroxyzine in human serum fortified samples. The test compounds were extracted from human serum by SPE using C18...

The chromatographic behavior of some basic and acidic drugs was studied on C18, Phenyl-Hexyl and Polar RP columns with methanol or acetonitrile as organic modifiers of aqueous mobile phases containing addition of diethylamine. Diethylamine plays a double function of silanol blocker reagent in analysis of basic drugs and ion-pair reagent in analysis...

Three independent reversed phase high-performance liquid chromatography (HPLC) procedures with diode array detection (DAD) for the analysis of carbamazepine (CBZ), topiramate (TPM), and valproic acid (VPA) have been developed in order to determine drug penetration of the blood–brain barrier. Determination of CBZ was performed on C18 column with mob...

Vortioxetine is a new drug against major depressive disorder with high affinity for a range of different serotonergic targets in the central nervous system. Therapeutic drug monitoring is an important tool for the clinical management of patients receiving a pharmacotherapy, particularly in psychiatry. For this reason, determination of drug concentr...

A high performance liquid chromatography (HPLC) method for simultaneous analysis of venlafaxine and its major metabolite O-desmethylvenlafaxine and vilazodone and its methabolite M10 have been developed and validated. Chromatography was performed on the Phenyl-Hexyl column with mobile phase containing methanol, acetate buffer at pH 3.5 and diethyla...

A simple, rapid and sensitive HPLC-DAD method has been developed and validated for the simultaneous determination of seven psychotropic drugs (risperidone, citalopram, clozapine,quetiapine, levomepromazine, perazine and aripiprazole) in human serum or salivasamples.The chromatographic analyses were performed on a XSELECT CSH Phenyl-Hexyl column wit...

Retention parameters of psychotropic drug standards were determined on different columns, i.e., Octadecyl silica, Phenyl, Phenyl-Hexyl, Polar Reverse Phase, Pentafluorophenyl, and Cyanopropyl using aqueous eluent systems containing methanol or acetonitrile as organic modifiers, acetate buffer at pH 3.5 and addition of silanol blocker − diethylamine...

Ion exchange chromatography, an alternative to reversed-phase (RP) chromatography, is described in this paper. We aimed to obtain optimal conditions for the separation of basic drugs because silica-based RP stationary phases show silanol effect and make the analysis of basic analytes hardly possible. The retention, separation selectivity, symmetry...

Selected psychotropic drug standards have been chromatographed on RP18, CN and diol layers with a variety of aqueous and nonaqueous mobile phases. The effect of buffers at acidic or basic pH, acetic acid, ammonia and diethylamine (DEA) in aqueous mobile phases on retention, efficiency and peak symmetry was examined. Improved peak symmetry and separ...

Retention parameters of psychotropic drug standards were determined on different stationary phases: octadecyl silica, polar octadecyl silica, cyanopropyl silica and phenyl-hexyl silica using aqueous eluent systems containing acetonitrile, methanol or mixture of acetonitrile and methanol as organic modifiers; acetic buffer at pH 3.5 and diethylamine...

Psychotropic drugs: desipramine, olanzapine and mitrazapine were chromatographed on cyanopropyl-silica and Diol-silica thin layers using various nonaqueous and aqueous eluents. The best results were obtained with addition of ammonia to nonaqueous eluent on both adsorbents. On the basis of the optimization, systems for extraction from human plasma a...

A general equation for determination of relative position, R g p, of solutes chromatographed under conditions of stepwise gradient elution with one void volume of mobile phase in reverse-phase thin-layer chromatography has been applied to separation of ten component dye mixture. A satisfactory agreement between calculated (by computer program) and...